Biostimulants to mitigate heat stress in soybean seed germination

Authors

DOI:

https://doi.org/10.5902/2179460X87738

Keywords:

Abiotic stress, Trichoderma, Plant growth promoter

Abstract

This study addresses the impact of high temperatures on soybean seed germination and proposes the use of biostimulants as a strategy to mitigate heat stress. The experiment adopted a completely randomized design, in a 4 x 3 bifactorial scheme (four combinations of biostimulants x three different temperatures). Parameters were analyzed to assess the physiological quality of the seeds, including the germination test, first count, germination speed index, root and shoot length, as well as the dry mass of the seedlings. The results indicated that biostimulants played an important role in promoting the initial germination of soybean seeds, even under different heat stress conditions. Both the isolated application of the AllRefresh® biostimulant and its association with Trichoderma harzianum demonstrated significant beneficial effects. These findings highlight the promising efficacy of biostimulants in improving the resistance of soybean seeds to heat stress, as well as contributing to the practical understanding of the application of these products, offering valuable insights for optimizing germination and initial seedling growth in challenging environments. The strategic use of biostimulants or plant stimulants can be a sustainable and effective tool to boost soybean productivity, especially in adverse temperature scenarios.

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Author Biographies

Kássia Silveira Crivellaro, Universidade Federal de Santa Maria

Master’s degree in Agrobiology from the Federal University of Santa Maria

Raquel Stefanello, Universidade Federal de Santa Maria

PhD in Agronomy from Federal University of Santa Maria. 

Sylvio Henrique Bidel Dornelles, Universidade Federal de Santa Maria

PhD in Agronomy from the Federal University of Santa Maria. 

Antonio Carlos Ferreira da Silva, Universidade Federal de Santa Maria

PhD in Sciences from the University of São Paulo.

Luciane Almeri Tabaldi, Universidade Federal de Santa Maria

PhD in Agronomy from the Federal University of Santa Maria.

Lucas Augusto da Silva Girio, Universidade Federal do Rio Grande do Sul

Doctor in Agronomy (Plant Production) from the Universidade Estadual Paulista Júlio de Mesquita Filho.

References

Allianza Química do Brasil Ltda. (2024). Allianza: A energia das plantas. Available in: https://www.allianzaquimica.com/.

Auge, G. et al. (2023). Plant environmental memory: implications, mechanisms and opportunities for plant scientists and beyond. AoB PLANTS, 15(4): plad032. DOI: https://doi.org/10.1093/aobpla/plad032

Bioagreen (2023). Available in: https://bioagreen.com.br/.

Box, G .E. P., & Cox, D. R. (1964). An analysis of transformations. Journal of the Royal Statistical Society: Serie B (Methodological), 26:211-243. DOI: https://doi.org/10.1111/j.2517-6161.1964.tb00553.x

Campobenedetto, C. et al. (2020). A biostimulant seed treatment improved heat stress tolerance during cucumber seed germination by acting on the antioxidant system and glyoxylate cycle. Frontiers in Plant Science, 11:836. DOI: https://doi.org/10.3389/fpls.2020.00836

Carvalho, B. L. et al. (2023). Use of plant regulators for activation of antioxidant enzymes in basil plants under water deficit conditions. Stresses, 3(1): 282-301. DOI: https://doi.org/10.3390/stresses3010021

Carvalho, N. M., & Nakagawa, J. (2012). Sementes: ciência, tecnologia e produção. 5.ed. Jaboticabal: FUNEP.

Chagas Junior, A. F. et al. (2022). Efficiency of Trichoplus (Trichoderma asperellum) as a plant growth promoter in soybean in the Cerrado field. Research, Society and Development, 11(5):e16111527970. DOI: https://doi.org/10.33448/rsd-v11i5.27970

Companhia Nacional de Abastecimento (2023). Acompanhamento da safra de grãos 2023/2024. Brasília: Conab. Available in: https://www.conab.gov.br/info-agro/safras/graos/boletim-da-safra-de-graos.

Companhia Nacional De Abastecimento (2020). Perspectivas para a agropecuária. Brasília: Conab. Available in: http://www.conab.gov.br.

Empresa de Assistência Técnica e Extensão Rural do Estado (Emater/RS) (2022). Estimativas Iniciais para a Safra 2022/2023. Available in: https://www.emater.tche.br/site/arquivos_pdf/safra/safraTabela_30082022.pdf.

Ferreira, E. B., Cavalcanti, P. P., & Nogueira, D. A. (2021). ExpDes.pt: Pacote Experimental Designs. R package version 1.2.2.

Floss, E. L. (2022). Maximizando o rendimento da soja: Ecofisiologia, nutrição e manejo. 3 ed. Passo Fundo: Aldeia Sul. Passografic.

Giordana, A. et al. (2023). Biostimulants derived from organic urban wastes and biomasses: An innovative approach. Frontiers in Chemistry, 10:11:969865. DOI: https://doi.org/10.3389/fchem.2023.969865

Governo Federal do Brasil (2020). O Programa Bioinsumos. Available in: https://www.gov.br/agricultura/pt-br/assuntos/inovacao/bioinsumos/o-programa.

Guan, Y. et al. (2009). Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Seed Science Center, 10(6):427-433. DOI: https://doi.org/10.1631/jzus.B0820373

Guirola-Céspedes, C, González-Suárez, E, & Ley-Chong, N. (2023). Design of a chemical plant to obtain phosphoric acid based on the study of the need for phosphate products in Cuba. Chemical Technology, 43(2): 406-420.

Hassan, M. U. et al. (2021). Estresse térmico em plantas cultivadas: natureza, impacto, mecanismos e estratégias de mitigação - uma revisão. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 155(2): 211-234. DOI: https://doi.org/10.1080/11263504.2020.1727987

Maguire, J. D. (1962). Speed of germination-aid selection and evaluation for seedling emergence and vigor. Crop Science, 2:176-177. DOI: https://doi.org/10.2135/cropsci1962.0011183X000200020033x

Márquez-Dávila, K. et al. (2020). Trichoderma and Clonostachys as biocontrol agents against Meloidogyne incognita in sacha inchi. Pesquisa Agropecuária Tropical, 50: e60890. DOI: https://doi.org/10.1590/1983-40632020v5060890

Marschner, H. (1995). Mineral nutrition of higher plants. Second edition. 889pp. London: AcademicPress. DOI: https://doi.org/10.1016/B978-012473542-2/50001-8

Martin, T. N. et al. (2023). Reunião de Pesquisa de Soja da Região Sul, 43. Atas e Resumos 2023. Santa Maria: UFSM. 660p.

Meyer, M. C., Mazaro, S. M., & Silva, J. C. (2019). Trichoderma: uso na agricultura. Londrina: Embrapa Soja.

Ministério da Agricultura, Pecuária e Abastecimento (2009). Regras para Análise de Sementes. MAPA. Secretaria de Defesa Agropecuária. Brasília, Mapa/ACS. 399p.

Nagasaki, Y. et al. (2024). Comparison of soybean crop performance under tropical environment between tropical and temperate cultivars with adjusted growth duration. Plant Production Science, 27(1):14-27. DOI: https://doi.org/10.1080/1343943X.2023.2299645

Nakagawa, J. Testes de vigor baseados em desempenho de plântulas (2020). In: Krzyzanowski, FC, Vieira, RD, França-Neto, JB, Marcos Filho, J. Vigor de sementes: conceitos e testes, 2020. Londrina: ABRATES.

ONU (2022). Os 17 objetivos de desenvolvimento sustentável no Brasil. Available in: https://brasil.un.org/pt-br/sdgs.

R Cor/E Team (2022). R: A Language and Environment for Statistical Computing. R Foundation for Statical Computing, Vienna, Austria. Available in: https://www.R-project.org

Sales, G. et al. (2023). Microbiolização de sementes de soja com Trichoderma harzianum: qualidade fisiológica e sanitária. Nativa, 11(2): 220-225. DOI: https://doi.org/10.31413/nat.v11i2.15234

Stanton, C. et al. (2021). Zinc in plants: Integrating homeostasis and biofortification. Molecular Plant, 15(1):65-85. DOI: https://doi.org/10.1016/j.molp.2021.12.008

Taiz, L. et al. (2017). Fisiologia Vegetal. 6. ed. Porto Alegre: Artmed.

Tian, X. Y. et al. (2021). Physiological and molecular advances in magnesium nutrition of plants. Plant Soil, 468:1-17. DOI: https://doi.org/10.1007/s11104-021-05139-w

Tyśkiewicz, R. et al. (2022). Trichoderma: the current status of its application in agriculture for the biocontrol of fungal phytopathogens and stimulation of plant growth. International Journal of Molecular Sciences, 23(4):2329. DOI: https://doi.org/10.3390/ijms23042329

Venables, W. N., & Ripley, B. D. (2022). Modern Applied statistics woth S. 4ª edição. Springer, New York.

Wickham, H. (2016). Ggplot2: Elegant grafics for data analysis. Springer-Verlag: New York, 2016. DOI: https://doi.org/10.1007/978-3-319-24277-4

Wilke, C. O. (2020). Cowplot: Streamlined plot theme and plot annotations for ‘ggplot2’. R package version 1.1.1.

Woo, S. L. et al. (2023). Trichoderma: um microrganismo multifuncional e benéfico para as plantas para a agricultura eco-sustentável. Nature Reviews Microbiology, 21:312–326.

Ye, J. Y., Tian, W. H., & Jin, C. W. (2022). Nitrogen in plants: from nutrition to the modulation of abiotic stress adaptation. Review, 2(4). DOI: https://doi.org/10.1007/s44154-021-00030-1

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Published

2025-06-11

How to Cite

Crivellaro, K. S., Stefanello, R., Dornelles, S. H. B., Silva, A. C. F. da, Tabaldi, L. A., & Girio, L. A. da S. (2025). Biostimulants to mitigate heat stress in soybean seed germination. Ciência E Natura, 47, e87738. https://doi.org/10.5902/2179460X87738

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