Trichoderma and Bradyrhizobium japonicum bioformulates on soy initial growth
DOI:
https://doi.org/10.5902/2179460X40873Keywords:
Glycine max, Microorganisms, Seed treatmentAbstract
Bioproducts can act on plant development in a positive, negative or neutral manner, as well as whether there is interaction of these products when used with other biological bioproducts, often used in soybeans such as Bradyrhizobium japonicum. The objective of this work was to evaluate the action of two trichoderma-based and two Bradyrhizobium japonicum-based bioformulates, combined or not, on variables in the early development of soybean plants, free of phytopathogens. The soybean cultivar Nidera 5909 was used and the treatments consisted of four commercial bioformulates, based on Trichoderma harzianum, Trichoderma sp. and two based on Bradyrhizobium japonicum, being tested in association and /or isolated for soybean seed inoculation. For the seedling emergence variable, there was no difference between the treatments tested. By analyzing the leaf variables such as leaf area, shoot length, fresh and dry shoot, the use of liquid B. japonicum results in higher averages compared to the other bioformulates. When evaluating root length, root surface area, root volume and root dry matter the use of bioformulated with Trichoderma sp. as active ingredient presented the highest averages. It was concluded that the use of Trichoderma sp. and Bradyrhizobium japonicum liquid in their isolated form, provide better initial development for soybean crop.
Downloads
References
AGUIAR, A.R.; AGUIAR, D.; TEDESCO, S.B. & SILVA, A.C.F. Efeito de metabólitos produzidos por Trichoderma spp. sobre o índice mitótico em células das pontas de raízes de Allium cepa. Bioscience Journal¬¬¬¬¬¬. 2015;31(3): 934-940.
AYOUBI, N.; ZAFARI, D. & MIRABOLFATHY, M. Combination of Trichoderma species and Bradyrhizobium japonicum in control of Phytophtora sojae and soybean growth. Journal Crop Protection. 2012;1(1):67-79.
AZARMI, R.; HAJIEGHRARI, B. & GIGLOU, A. Effect of Trichoderma isolates on tomato seedling growth response and nutrient uptake. African Journal of Biotechnology. 2011;10(31): 5850-5855.
BÁBARO, I.M.; MACHADO, P.C.; BÁRBARO JUNIOR, L.S.; TICELLI, M.; MIGUEL, F.B. & SILVA, J.A.A. Produtividade da soja em resposta à inoculação padrão e co-inoculação. Colloquium Agrariae. 2009;5(1): 01-07.
BENÍTEZ, T.; RINCÓN, A.M.; LIMÓN, M. C.; CODÓN, A. C. Biocontrol mechanisms of Trichoderma strains. International Microbiology. 2004;77: 249-260.
BRASIL. Ministério da Agricultura, Pecuária e Abastecimento. Regras para análise de sementes. Secretaria de Defesa Agropecuária - MAPA/ACS. 2009: 395-399.
BUSO, P.H.M. Contribuição de micro-organismos promotores do crescimento vegetal coinoculados na cultura da soja. Tese de Doutoramento. Curitiba, Universidade Federal do Paraná; 2017. 94 p.
CHAGAS, L.F.B.; CHAGAS JUNIOR, A.F.; SOARES, L.P. & FIDELIS, R.R. Trichoderma na promoção de crescimento vegetal. Revista Agricultura Neotropical. 2017;4(3): 97-102.
CASSÁN, F.; PERRIG, D.; SGROY, V.; MASCIARELLI, O.; PENNA, C. & LUNA, V. Azospirillum brasiliense Az39 and Bradyrhizobium japonicum E109, inoculated singly or in combination. Promote seed germination and early seedling growth in corn (Zea mays L.) and soybean Glycine max L.). European Journal of Soil Biology. 2009; 45(1): 28-35.
CONAB. Companhia Nacional de Abastecimento. Acompanhamento da Safra Brasileira: grãos. (Terceiro levantamento, p.1 – 127). Brasília, DF: CONAB. 2018.
DELABONA, P.S.; PIROTA, R.D.P.B.; CODIMA, C.A.; TREMACOLDI, C.R.; RODRIGUES, A.; FARINAS, C.S. Using Amazon Forest Fungi and Agricultural Residues as a Strategy to Produce Cellulolytic Enzymes. Biomass and Bioenergy 2012; 37:243-250.
EGAMBERDIEVA, D. & JABBOROVA, D. Synergistic interactions between Bradyrhizobium japonicum and the endophyte Stenotrophomonas rhizophila and their effects on growth, and nodulation of soybean under salt stress. Plant Soil. 2016;45(1-2):35-45.
ESPÍDOLA, C.J. & CUNHA, R.C.C. A dinâmica geoeconômica recente da cadeia produtiva da soja no Brasil e no mundo. GeoTextos. 2015;11(1):217-238.
FRACETO, L.F.; MARUYAMA, C.R.; GUILER, M.; MISHRA, S.; KESWANI, C.; SINGH, H.B.; LIMA, R. Trichodema harzianum-base novel formulations: potential applications for management of Next-Generation agricultural challenges. Journal of Chemical Technology & Biotechnology. 2018;93(8): 2056-2063.
FERREIRA, D.F. Sisvar: um guia para seus procedimentos de Bootstrap em múltiplas comparações. Ciência e Agrotecnologia. 2014;38(2):109-112.
GEMIN, L. G.; DATSCH, R.; MÓGOR, A. F. & MÓGOR, G. Biofertilizer effect of yeast fermented broth on organic tomato seedlings. Revista de ciências agrárias. 2018;41(2):424-431.
GOKUL, B.; LEE, J.H.; SONG, K.B.; RHEE, S.K.; KIM, C.H.; PANDA, T. Characterization and applications of chitinases from Trichoderma harzianum – A review. Bioprocess Engineering. 2000;23(6):691-694.
GRANADOS, C.J.B.; PÉREZ, A.B.P.; CODOVI, U.A.; SANZ, M.Q.; RODRÍGUEZ, Y.G.; NÁPOLES, F.M. & VALDÉS, A.M. Efecto de la inoculación com Bradyrhizobium sp. y Trichoderma harzianum em triticale (X. Triticosecale Wittmack), em condiciones de estrés por sequía. Pastos y Forrajes. 2016;39(1):19-26.
HUNGRIA, M.; MENDES, I.C. & MERCANTE, F.M. (2013) - A fixação biológica do nitrogênio como tecnologia de baixa emissão de carbono para as culturas do feijoeiro e da soja. Embrapa Soja. https://www.embrapa.br/busca-de-publicacoes/-/publicacao/964424/a-fixacao-biologica-do-nitrogenio-como-tecnologia-de-baixa-emissao-de-carbono-para-as-culturas-do-feijoeiro-e-da-soja. 10 Jun. 2018.
JUGE, C.; PRÉVOST, D.; BERTRAND, A.; BIPFUBUSA, M. & CHALIGOUR, F.P. Growth and biochemical responses of soybean to double and triple microbial associations with Bradyrhizobium, Azospirillum and arbuscular mycorrhizae. Aplied Soil Ecology. 2012;61(1): 147-157.
KOZUSNY-ANDREANI, D.I. & ANDREANI JUNIOR, R. Colonização rizosférica e promoção do crescimento por rizóbios em mudas de alface. Nucleus. 2014;11(2):443-452.
KUMARI, A.; KUMAR, R.; MAURYA, S.; PANDEY. P. K. Antagonistic potential of different isolates of Trichoderma against Rhizoctonia solani. European Journal of Experimental Biology. 2016;6(2): 1-6.
LEELAVATHI, M.S.; VANI, L.; REENA, P. Antimicrobial activity of Trichoderma harzianum against bacteria and fungi. International Journal of Current Microbiology and Applied Sciences. 2014;3(1): 96-103.
MACHADO, D.F.M.; PARZIANELLO, F.R.; SILVA, A.C.F. & ANTONIOLLI, Z.I. Trichoderma no Brasil: O fungo e o bioagente. Revista de Ciências Agrárias. 2012;35(1):274-288.
MATHIAS, V.; PEREIRA, T.; MANTOVANI, A.; ZÍLIO, M.; MIOTTO, P. & COELHO, C.M.M. Implicações da época de colheita sobre a qualidade fisiológica de sementes de soja. Revista Agro@mbiente on-line. 2017;11(3):223-231.
MATTOS, M. Promoção de crescimento de soja a partir da inoculação de sementes com microrganismos não noduladores. Dissertação de Mestrado. Cerro Largo, Universidade Federal da Fronteira Sul; 2017. 47 p.
NETO, F.A.; PACHECO, L.P.; ARAÚJO, A.S.F.; PETTER, F.A.; ALMEIDA, F.A. & ALBUQUERQUE, J.A. Tempo de contato e de combinações de fungicidas, aditivo e inoculante sobre a sobrevivência de rizóbios e nodulação da soja. Revista Agro@mbiente on-line.2014;8(1):149-154.
RUFINI, M.; OLIVEIRA, D.P.; TROCHMANN, A.; SOARES, B.L.; ANDRADE, M.J.B. & MOREIRA, F.M.S. Estirpes de Bradyrhizobium em simbiose com guandu-anão em casa de vegetação e no campo. Revista Pesquisa Agropecuária Brasileira. 2014;49(3):197-206.
SANTOS, K.C.; UCHÔA, S.C.P.; MELO, V.F.; ALVES, J.M.A.; ROCHA, P.R.R. & XIMENES, C.K.S. Inoculação com Bradyrhizobium e adubação nitrogenada em feijão-caupi cultivado em diferentes solos. Revista AgroAmbiente On-line. 2014;8(3): 306-317.
SILVA, L.R.; PEREIRA, M.J.; AZEVEDO, J.; MULAS, R.; VELAZQUEZ, E.; GONZÁLES-ANDRÉS, F.; VALENTÃO, P.; ANDRADE, P.B. Inoculation with Bradyrhizobium japonicum enhances the organic and fatty acids contente of soybean (Glycine max (L.) Merril) seeds. Food Chemistry. 2013;141(14): 3636-3648, 2013.
VINALE, F.; FLEMATTI, G.; SIVASITHAMPARAM, K.; LORITO, M.; MARRA, R.;
SKELTON, B.W.; GHISALBERTI, E.L. Harzianic Acid, an Antifungal and Plant Growth Promoting Metabolite from Trichoderma harzianum. Journal of Natural Products. 2009;72(11): 2032-2035.
YEDIDIA, I.; SRIVASTVA, A.K.; KAPULNIK, Y. & CHET, I. Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants. Plant and Soil. 2001;235(2):235‑242.
ZIMMER, S.; MESSMER, M.; HAASE, T.; PIEPHO, H.P.; MINDERMANN, A.; SCHILZ, H.; HABEKU, A.; ORDON, F.; WILBOIS, K.P.; HEB, J. Effects of soybean variety and Bradyrhizobium strains on yield, protein content and biological nitrogen fixation under cool growing conditions in Germany. European Journal of Agronomy. 2016;72: 38-46.
Published
How to Cite
Issue
Section
License
To access the DECLARATION AND TRANSFER OF COPYRIGHT AUTHOR’S DECLARATION AND COPYRIGHT LICENSE click here.
Ethical Guidelines for Journal Publication
The Ciência e Natura journal is committed to ensuring ethics in publication and quality of articles.
Conformance to standards of ethical behavior is therefore expected of all parties involved: Authors, Editors, Reviewers, and the Publisher.
In particular,
Authors: Authors should present an objective discussion of the significance of research work as well as sufficient detail and references to permit others to replicate the experiments. Fraudulent or knowingly inaccurate statements constitute unethical behavior and are unacceptable. Review Articles should also be objective, comprehensive, and accurate accounts of the state of the art. The Authors should ensure that their work is entirely original works, and if the work and/or words of others have been used, this has been appropriately acknowledged. Plagiarism in all its forms constitutes unethical publishing behavior and is unacceptable. Submitting the same manuscript to more than one journal concurrently constitutes unethical publishing behavior and is unacceptable. Authors should not submit articles describing essentially the same research to more than one journal. The corresponding Author should ensure that there is a full consensus of all Co-authors in approving the final version of the paper and its submission for publication.
Editors: Editors should evaluate manuscripts exclusively on the basis of their academic merit. An Editor must not use unpublished information in the editor's own research without the express written consent of the Author. Editors should take reasonable responsive measures when ethical complaints have been presented concerning a submitted manuscript or published paper.
Reviewers: Any manuscripts received for review must be treated as confidential documents. Privileged information or ideas obtained through peer review must be kept confidential and not used for personal advantage. Reviewers should be conducted objectively, and observations should be formulated clearly with supporting arguments, so that Authors can use them for improving the paper. Any selected Reviewer who feels unqualified to review the research reported in a manuscript or knows that its prompt review will be impossible should notify the Editor and excuse himself from the review process. Reviewers should not consider manuscripts in which they have conflicts of interest resulting from competitive, collaborative, or other relationships or connections with any of the authors, companies, or institutions connected to the papers.
