YEASTS: DIVERSITY IN KEFIR, PROBIOTIC POTENTIAL AND POSSIBLE USE IN ICE CREAM.
DOI:
https://doi.org/10.5902/2179460X19749Keywords:
Kefir. Yeast. Probiotic. Saccharomyces boulardii. Ice cream.Abstract
Kefir is a product made by the symbiotic fermentation of different microrganisms, resulting in a product with microbiological characteristics, physicochemical and sensory unparalleled. Bacterias, mainly of the gene Lactobacillus, and the yeasts are the main microrganisms that give rise of this product. The genres Kluyveromyces and Saccharomyces are the largest representatives of the yeasts found at Kefir’s microbiot. In relation of the probiotic potential of the yeasts, the one sold in drug form by mouth, is the Saccharomyces boulardii, however, researches are showing that other genres, other species and new strains of yeasts also had promising probiotic potential, but the researches don’t tell the application of this new probiotic yeasts in food. The aim of this review involved the achievement of a bibliographic review about different species and strains of yeasts found at Kefir’s samples by differents authors at the global level. The probiotic potential of yeasts was also studied, as well as the capability of probiotic microrganisms being added to ice cream. With conclusion it can be said that countless strains of yeasts are present in the Kefir’s symbiotic complex and, some of this possibly submit probiotic potential, that could being incorporated into ice cream, once the food have wide consumer acceptance and don’t uses the fermentation process in it’s preparation.
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References
ALAMPRESE, C. et al. Effects of Lactobacillus rhamnosus GG addition in ice cream. International Journal Dairy Technol., v.58, p.200-206, 2005.
ALAMPRESE, C. et al. Survival of Lactobacillus johnsonii La1 and influence of its addition in retail-manufactured ice cream produced with different sugar and fat concentrations. International Dairy Journal, v.12, p.201-208, 2002.
ANVISA – AGÊNCIA NACIONAL DE VIGILÂNCIA SANITÁRIA. Alimentos com Alegações de Propriedades Funcionais e ou de Saúde, Novos Alimentos/Ingredientes, Substâncias Bioativas e Probióticos: lista de alegações de propriedades funcionais aprovadas. Atualizado em julho de 2008. Disponível em:http://www.anvisa.gov.br/alimentos/comissoes/tecno_lista_alega.htm. Acessado em 02/09/2015.
ARSLAN, S. et al. Microencapsulation of probiotic Saccharomyces cerevisiae var. boulardii with different wall materials by spray drying. LWT - Food Science and Technology. v. 63, n. 1, p. 685–690, 2015.
ARROYO-LÓPEZ, F. N. Yeasts in table olive processing: Desirable or spoilage microorganisms? International Journal of Food Microbiology, v.160, p. 42–49, 2012.
BEVILACQUA, A. et al. Technological and spoiling characteristics of the yeast microflora isolated from Bella Di Cerignola table olives. International Journal of Food Science and Technology, v. 44, p. 2198-2207, 2009.
BASYIGIT, G. et al. Viability of human-derived probiotic lactobacilli in ice cream produced with sucrose and aspartame. Journal Microbiology Biotechnology, v.33, p.796-800, 2006.
BERGMANN, R. S. O. et al., Microbial profile of a kefir sample preparations – grains in natura and lyophilized and fermented suspension. Ciência e Tecnologia de Alimentos, v. 30, n. 4, p. 1022-1026, 2010.
CASTRO-CISLAGHI, F. P. et al. Bifidobacterium Bb-12 microencapsulated by spray drying with whey: Survival under simulated gastrointestinal conditions, tolerance to NaCl, and viability during storage. Journal of Food Engineering, v.113, p.186-193, 2012.
CHAMPAGNE, C. P. et al. Challenges in the addition of probiotic cultures to foods. Critical Reviews in Food Science and Nutrition, v. 45, n. 1, p. 61–84, 2005.
CHIFIRIUC, M. C. et al. In vitro assay of the antimicrobial activity of kephir against bacterial and fungal strains. Anaerobe, v.17, p.433-435, 2011.
CHEN, P. et al. Screening for potential new probiotic based on probiotic properties and α-glucosidase inhibitory activity. Food Control, v. 35, p. 65-72, 2014.
COUSIN, F. J. et al. The first dairy product exclusively fermented by Propionibacterium freudenreichii: A new vector to study probiotic potentialities in vivo. Food Microbiology, v. 32, p. 135-146, 2012.
CRUZ, A. G. et al. Sorvetes probióticos e prebióticos. In: SAAD, S. M. I.; CRUZ, A. G.; FARIA, J. A. F. (Org.). Probióticos e prebióticos em alimentos: Fundamentos e Aplicações Tecnológicas. São Paulo: Livraria Varela. cap 15, p. 359-388. 2011.
CRUZ, A. G. et al. Ice-cream as a probiotic food carrier. Food Research International, v. 42, p. 1233-1239, 2009.
CZERUCKA, D. et al. Review article: yeast as probiotics – Saccharomyces boulardii. Aliment. Pharmacol. Ther., v. 26, n. 767–778, 2007.
DIOSMA, G. et al. Yeasts from kefir grains: isolation, identification, and probiotic characterization. World Journal Microbiol Biotechnol. p. 1-11, 2013.
DUONGTHINGOC, D. et al. Effect of whey protein agglomeration on spray dried microcapsules containing Saccharomyces boulardii. Food Chemistry, v. 141, n. 3, p. 1782-178, 2013.
ERTEKIN, B.; GUZEL-SEYDIM, Z. B. Effect of fat replacer on kefir quality. Journal of the Science of Food and Agriculture. v. 90, p. 543-548, 2009.
FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS, WORLD HEALTH ORGANIZATION. CODEX Standard for Fermented Milks. Codex Stan, second ed. 2003.
FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS, WORLD HEALTH ORGANIZATION. Evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Córdoba, 2002. 34p.
FARNWORTH, E. R. Kefir – a complex probiotic. Food Science e Technology Bulletin: Functional Foods, v. 2, p. 1-17, 2005.
FERREIRA, I. M. P. L. V. O. et al. Short communication: Effect of kefir grains on proteolysis of major milk proteins. Journal of Dairy Science. v. 93, p. 27-31, 2010.
FLEET, G. H. Yeasts in foods and beverages: impact on product quality and safety. Curr. Opin. Biotechnol. v.18, p. 170–175, 2007.
GARCÍA-HERNÁNDEZ, Y. Identification and in vitro screening of avian yeasts for use as probiotic. Research in Veterinary Science, v. 93, p. 798–802, 2012.
GOLOWCZYC, M. A., et al. Cellular injuries of spray-dried Lactobacillus spp. isolated from kefir and their impact on probiotic properties. International Journal of Food Microbiology, v.144, p. 556–560, 2011.
GULITZ, A. The microbial diversity of water kefir. International Journal of Food Microbiology, v. 151, n. 3, p. 284-288, 2011.
GUZEL-SEYDIM, Z. et al. Review: functional properties of kefir. Critical Reviews in Food Science and Nutrition, v. 51, p. 261–268, 2011.
HOMAYOUNI, A. et al. Effect of microencapsulation and resistant starch on the probiotic survival and sensory properties of synbiotic ice cream. Food Chemistry, v. 111, p. 50–55, 2008.
JIANZHONG, Z. et al. Analysis of the microflora in Tibetan kefir grains using denaturing gradient gel electrophoresis. Food Microbiology. v. 26, p. 770–775, 2009.
JACQUES, N.; CASAREGOLA, S. Safety assessment of dairy microorganisms: The hemiascomycetous yeasts. International Journal Food Microbiology. v. 126, p. 321–326, 2008.
KOMATSU, T. R. et al. Inovação, persistência e criatividade superando barreiras no desenvolvimento de alimentos probióticos. Revista Brasileira de Ciências Farmacêuticas, v. 44, n. 3, 2008.
LEITE, A. M. O., et al., Assessment of the microbial diversity of Brazilian kefir grains by PCR-DGGE and pyrosequencing analysis. Food Microbiology. v. 31, p.215-221, 2012.
LOPITZ-OTSOA, F. et al. Kefir: A symbiotic yeasts-bacteria community with alleged healthy capabilities. Rev Iberoamerican Micology, v. 23, p. 67-74, 2006.
MALEKI, D. et al. Probiotics in Cancer Prevention, Updating the Evidence. Probiotics, Prebiotics, and Synbiotics. Bioactive Foods in Health Promotion 2016, Pages 781–791.
MARTINS, F. S. et al. Screening of yeasts as probiotic based on capacities to colonize the gastrointestinal tract and to protect against enteropathogen challenge in mice. Journal Gen. Appl. Microbiol, v. 51, p. 83-92, 2005a.
MARTINS, F. S.; et al. Estudo do potencial probiótico de linhagens de Saccharomyces cerevisiae através de testes in vitro. Revista de biologia e ciências da terra, v. 5, p. 14-20, 2005b.
MARTINS, F. S. et al. Saccharomyces cerevisiae strain 905 reduces the translocation of Salmonella enterica serotype Typhimurium and stimulates the immune system in gnotobiotic and conventional mice. Journal Med. Microbiology, v. 56, p. 352–359, 2007.
MARTINS, F. S., et al. Inhibition of tissue inflammation and bacterial translocation as one of the protective mechanisms of Saccharomyces boulardii against Salmonella infection in mice. Microbes and Infection, v. 15, p. 270-279, 2013.
MCFARLAND, L. V. Systematic review and meta-analysis of Saccharomyces boulardii in adult patients. World Journal Gastroenterology. v. 16, n. 18, p. 2202-2222, 2010.
MIGUEL, M. G. C. P. et al. Diversity of bacteria present in milk kefir grains using culture-dependent and culture-independent methods. Food Research International, v. 42, p. 1523-1528, 2010.
NAMBOU, K. et al. A novel approach of direct formulation of defined starter cultures for different kefir-like beverage production. International Dairy Journal, v. 34, n. 2, p. 237-246, 2014.
PEDERSEN, L. L. et al. Biodiversity and probiotic potential of yeasts isolated from Fura, a West African spontaneously fermented cereal. International Journal of Food Microbiology. v. 159, p. 144–151, 2012.
PENNACCHIA, C., et al. Isolation of Saccharomyces cerevisiae strains from different food matrices and their preliminary selection for a potential use as probiotics. Journal of Applied Microbiology, v. 105, p. 1919-1928, 2008.
PERITI, P.; TONELLI, F. Preclinical and clinical p.harmacology of biotherapeutic agents: Saccharomyces boulardii. J Chemother. v. 13, p. 473–493, 2001.
PERRICONE, M. Technological characterization and probiotic traits of yeasts isolated from Altamura sourdough to select promising microorganisms as functional starter cultures for cereal-based products. Food Microbiology, v. 38, p. 26-35, 2014.
PLESSAS, S. et al. Bread making using kefir grains as baker’s yeast. Food Chemistry, v. 93, p. 585–589, 2005.
ROMANIN, D. et al. Downregulation of intestinal epithelial innate response by probiotic yeasts isolated fron kefir. Int J Food Microbiology, v. 140, p. 102–108, 2010.
SAAD, S. M. I. Probiotics and prebiotics: The state of the art. Brazilian Journal of Pharmaceutical Sciences, v. 42, n. 1, p. 1–16, 2006.
SAAD, S. M. I. et al. Probióticos e prebióticos em alimentos. Fundamentos e aplicações tecnológicas. 1. Ed. São Paulo, Livraria Varela, 2011.
SAAD, N. An overview of the last advances in probiotic and prebiotic Field. LWT - Food Science and Technology. v. 50, p. 1-16, 2013.
SARKAR, S. Potential of kefir as a dietetic beverage – a review. British Journal of Nutrition, v. 109, p. 280-290, 2007.
SILVA, P. D L da et al. Potentially probiotic ice cream from goat's milk: Characterization and cell viability during processing, storage and simulated gastrointestinal conditions. LWT - Food Science and Technology. v. 62, n. 1, part 2, 2015, p. 452–457, 2015.
SILVA, T. et al. Characterization of yeasts from Portuguese brined olives, with a focus on their potentially probiotic behavior. LWT- Food Science and Technology, v. 44, p. 1349–1354, 2011.
STADIE, J. et al. Metabolic activity and symbiotic interactions of lactic acid bacteria and yeasts isolated from water kefir. Food Microbiology, v. 35, p. 92-98, 2013.
SURIASIH, K. Microbiological and Chemical Properties of Kefir Made of Bali Cattle Milk. Food Science and Quality Management. v. 6, 2012.
TAMIME, A. Y. et al. Properties of kefir made in Scotland and Poland using bovine, caprine and ovine milk with different starter cultures. Lebensm.-Wiss. u.-Technol., v. 34, p. 251-261, 2001.
TAS¸ T. B. K. K. et al. Identification of microbial flora in kefir grains produced in Turkey using PCR. International Journal of Dairy Technology, v. 65, n. 1, p. 126-131, 2012.
THOMAS, M. B. et al. Enhanced viability of probiotic Saccharomyces boulardii encapsulated by layer-by-layer approach in pH responsive chitosan–dextran sulfate polyelectrolytes. Journal of Food Engineering, v. 136, p. 1–8, 2014.
URAZ, G. et al. Isolation of yeast from microflora of kefir. Abstracts / Journal of Biotechnology. v. 161S, p. 19–48, 2012.
VINDEROLA, C. G. et al. Immunomodulating capacity of kefir. Journal Dairy Reserch, v.72, p.195-202, 2005a.
VINDEROLA, C. G. et al. Distal mucosal site stimulation by kefir and duration of the immune response. European Journal Inflamm. v. 3, p. 63–73, 2005b.
VINDEROLA, C. G. et al. Effects of kefir fractions on innate immunity. Immunobiology, v. 211, p. 149–156, 2006.
WANG, S-Y., et al. Investigation of microorganisms involved in biosynthesis of the kefir grain. Food Microbiology, v. 32, p. 274-285, 2012.
WESCHENFELDER, S.; WIEST, J. M.; CARVALHO, H. H. C. Atividade anti-Escherichia coli em kefir e soro de kefir tradicionais. Rev. Inst. Latic. “Cândido Tostes”, v. 34, n 367/368, p. 48-55, 2009.
WITTHUHN, R. C. et al. Impact of preservation and different packaging conditions on the microbial community and activity of kefir grains. Food Microbiology, v.22, p.337-344, 2004.
WITTHUHN, R.C. et al. Characterisation of the microbial population at different stages of Kefir production and Kefir grain mass
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