Chemical characterization and biotechnological potential of Salvia rosmarinus Spenn essential oil nanoemulsions
DOI:
https://doi.org/10.5902/2179460X73690Keywords:
Salvia rosmarinus, Nanoemulsions, Essential oilAbstract
This study aimed to determine the total phenolic compounds, evaluate the antioxidant and anti-inflammatory activities of nanoemulsions (O/W) and essential oils (EOs) from Salvia rosmarinus (rosemary). The plant material was obtained in the city of São Luís (MA). The EO was obtained by the hydrodistillation technique in a modified Clevenger extractor and the NOE's by phase inversion. The chemical constituents of EO were determined by GC-MS. The determination of total phenolic compounds (TPC) was performed by the Folin-Ciocalteu method. The anti-inflammatory activity was performed by the method of protein denaturation and the antioxidant activity by the spectrophotometric method of scavenging hydroxyl radicals. The GC-MS allowed quantifying 1,8-cineol (30.22%), α-pinene (22.14%), camphor (18.33%) and camphene (10.36%) as major components of the EO. The TPC of the EO was quantified at 26.74 mg EAT g-1 and the refractive index at 1.466 nD 25°. In the antioxidant activity test, an EC50 of 80.33 mgL-1 was obtained for the EO and from 19.56 to 408.85 mg L-1 for the nanoemulsions. In the anti-inflammatory activity assay, an EC50 of 62.46 mgL-1 was obtained for EO and 64.96 to 4220.25 mg L-1 for NOE's. Finally, the pharmacological activities tested showed efficient values for EC50, therefore considered active. This activity is attributed to its chemical compounds present, thus encouraging studies with this species aiming at its potential application in a formulated bioproduct.
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