Physicochemical properties, toxicity and larvicidal activity of the essential oil of Cymbopogon winterianus in front of Aedes aegypti

Gabriela Morais da Costa, Maria Conceição Carneiro dos Santos, Gustavo Oliveira Everton, Paulina de Cássia Duarte de Sousa, Maurício Eduardo Salgado Rangel

Abstract


Due to the increasing rate of morbidity and mortality caused by vector diseases in the current context, especially by Aedes aegypti, substances of plants have been studied as alternatives to chemical insecticides, among them, the essential oil (EO) of the species Cymbopogon winterianus. Thus, this study evaluated the larvicidal activity of the EO of C. winterianus against the A. aegypti larvae. EO was extracted through the hydro-distillation technique and physicochemical properties were determined. To evaluate larvicidal activity, tests were performed with larvae in the third instar at the final concentrations of 19.54; 26.50; 55.59; 138.98; 208.47 and 277.97 mg L-1 of C. winterianus EO. In addition, Artemia salina Leach bioassay was used to verify toxicity effect. EO obtained presented satisfactory results  in 2.64%. In the larvicidal assay, 100% mortality of larvae was observed after 24 hours at concentrations of 208.47 mg L-1 and 277.97 mg L-1 of the EO, showed effective in the other concentrations and with LC50 of 46.18 mg L-1, considered highly active. In the toxicity assay, the EO presented LC50 at 532.34 mg L-1, showed considered nontoxic. These results reinforce the use of EO front A. aegypt larvae control.

 


Keywords


Essential oil; Larvicidal activity; Aedes aegypti; Cymbopogon winterianus

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AGNOLIN, C. A.; OLIVO, C. J.; PARRA, C. L. C.; AGUIRRE, P. F; BEM, C. M. D.; ZENI, D.; MOREL, A. F. Eficácia acaricida do óleo de citronela contra o Rhipicephalus (Boophilus) microplus. Revista Brasileira de Saúde e Produção Animal, v. 15, n. 3, p. 604-612, 2014.

AMER, A.; MEHLHORN, H. Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera, Culicidae). Parasitology Research, v. 99, n. 4, p. 466-472, 2006.

BLANK, A. F.; COSTA, A. G.; ARRIGONI-BLANK, M. D. F.; CAVALCANTI, S. C.; ALVES, P. B.; INNECCO, R.; EHLERT, P. A. D.; SOUSA, I. F. D. Influence of season, harvest time and drying on Java citronella (Cymbopogon winterianus Jowitt) volatile oil. Revista Brasileira de Farmacognosia, v. 17, n. 4, p. 557-564, 2007.

BRASIL. Farmacopeia Brasileira. 6.ed. Volume 1. Agência Nacional de Vigilância Sanitária. Brasília: Anvisa. 2019.

BRASILEIRO, B. G.; PIZZIOLO, V. R.; RASLAN, D. S.; JAMAL, C. M.; SILVEIRA, D. Antimicrobial and cytotoxic activities screening of some Brazilian medicinal plants used in Governador Valadares district. Revista Brasileira de Ciências Farmacêuticas, v. 42, n. 2, p. 195-202, 2006.

CANTANE, D. R.; CRISTINO, A. C.; OLIVEIRA, R. A.; FLORENTINO, H.; SANTOS, F. L.; FERNANDES, M. A.; SOUZA NETO, J. A.; RIBOLLA, P. O desenvolvimento da população do Aedes aegypti aplicado ao modelo de otimização no controle da Dengue. XLVII SBPO-Simpósio Brasileiro de Pesquisa Operacional, 2015.

CARVALHO, A. F. U.; MELO, V. M. M.; CRAVEIRO, A. A.; MACHADO, M. I. L.; BANTIM, M. B.; RABELO, E. F. Larvicidal activity of the essential oil from Lippia sidoides Cham. against Aedes aegypti Linn. Memórias do Instituto Oswaldo Cruz, v. 98, n. 4, p. 569-571, 2003.

CASSEL, E.; VARGAS, R. M. F.; MARTINEZ, N.; LORENZO, D.; DELLACASSA, E. Steam distillation modeling for essential oil extraction process. Industrial crops and products, v. 29, n. 1, p. 171-176, 2009.

CAVALCANTI, E. S. B.; MORAIS, S. M. D.; LIMA, M. A. A.; SANTANA, E. W. P. Larvicidal activity of essential oils from Brazilian plants against Aedes aegypti L. Memórias do Instituto Oswaldo Cruz, v. 99, n. 5, p. 541-544, 2004.

CHENG, S. S.; CHANG, H. T.; CHANG, S. T.; TSAI, K. H.; CHEN, W. J. Bioactivity of selected plant essential oils against the yellow fever mosquito Aedes aegypti larvae. Bioresource Technology, v. 89, n. 1, p. 99-102, 2003.

CHENG, S. S.; LIU, J. Y.; TSAI, K. H.; CHEN, W. J.; CHANG, S. T. Chemical composition and mosquito larvicidal activity of essential oils from leaves of different Cinnamomum osmophloeum provenances. Journal of Agricultural and Food Chemistry, v. 52, n. 14, p. 4395-4400, 2004.

CHUNG, I. M.; SEO, S. H.; KANG, E. Y.; PARK, S. D.; PARK, W. H.; MOON, H. I. Chemical composition and larvicidal effects of essential oil of Dendropanax morbifera against Aedes aegypti L. Biochemical Systematics and Ecology, v. 37, n. 4, p. 470-473, 2009.

CORRÊA, J. C. R.; SALGADO, H. R. N. Atividade inseticida das plantas e aplicações: revisão. Revista Brasileira de Plantas Medicinais, v. 13, n. 4, p. 500-506, 2011.

CRUZ, R. C. D.; SILVA, S. L. C. E.; SOUZA, I. A.; GUALBERTO, S. A.; CARVALHO, K. S.; SANTOS, F. R.; CARVALHO, M. G. Toxicological evaluation of essential oil from the leaves of Croton argyrophyllus (Euphorbiaceae) on Aedes aegypti (Diptera: Culicidae) and Mus musculus (Rodentia: Muridae). Journal of medical entomology, v. 54, n. 4, p. 985-993, 2017.

DA CRUZ, T. P.; ALVES, F. R.; MENDONÇA, R. F.; COSTA, A. V.; DE JESUS JUNIOR, W. C.; PINHEIRO, P. F.; MARINS, A. K. Atividade fungicida do óleo essencial de Cymbopogon winterianus jowit (Citronela) contra Fusarium solani. Bioscience Journal, v. 31, n. 1, 2015.

DOLABELLA, M. F. Triagem in vitro para a atividade antitumoral e anti-T. cruzi de extratos vegetais, produtos naturais e sintéticos. 1997. Tese de Doutorado. tese de mestrado, Belo Horizonte Universidade Federal de Minas Gerais.

EDEN, W. T.; ALIGHIRI, D.; CAHYONO, E.; SUPARDI, K. I.; WIJAYATI, N. Fractionation of Java citronella oil and citronellal purification by batch vacuum fractional distillation. In: IOP Conference Series: Materials Science and Engineering. Indonesia, 2018. p. 012067.

FALCÃO L. Experimentais, em diferentes modelos. Universidade Regional Integrada do Alto Uruguai e das Missões URI- Campus de Erechim. Departamento de Ciências Biológicas Programa de Pós-Graduação em Ecologia.

FANSIRI, T.; THAVARA, U.; TAWATSIN, A.; KRASAESUB, S.; SITHIPRASASNA, R. Laboratory and semi-field evaluation of mosquito dunks® against Aedes aegypti and Aedes albopictus larvae (Diptera: Culicidae). Southeast Asian journal of tropical medicine and public health, v. 37, n. 1, p. 62, 2006.

FARPEJ. Armadilha letal para mosquitos, temperada com atitude de civilidade. 2013. Disponível em: . Acesso em: 30 de jan. de 2020.

FERREIRA, M. D. S.; BATISTA, E. K. F., DOS SANTOS FARIAS, I., Santos, L. F., de Oliveira, J. M. G., & de Sousa Silva, S. M. M. Avaliação fitoquímica e toxicológica dos extratos do fruto de Buchenavia sp. Acta Brasiliensis, v. 1, n. 2, p. 17-22, 2017.

FIAZ, M.; MARTÍNEZ, L. C.; PLATA-RUEDA A.; GONÇALVES, W. G.; DE SOUSA, D. L. L.; COSSOLIN, J. F. S.; SERRÃO, J. E. Pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of Aedes aegypti larvae. PeerJ, v. 7, p. e7489, 2019.

EPA - Environmental Protection Agency. FEDERAL INSECTICIDE, FUNGICIDE, AND RODENTICIDE ACT (FIFRA) AND FEDERAL FACILITIES. Disponível em: . Acesso em: 10 de abr. de 2020.

GOMES, P. R. B.; SILVA, A. L. S.; PINHEIRO, H. A.; CARVALHO, L. L.; LIMA, H. S.; SILVA, E. F.; OLIVEIRA, M. B. Avaliação da atividade larvicida do óleo essencial do Zingiber officinale Roscoe (gengibre) frente ao mosquito Aedes aegypti. Revista brasileira de plantas medicinais, v. 18, n. 2, p. 597-604, 2016.

GOMES, P. R. B.; OLIVEIRA, M. B.; DE SOUSA, D. A.; DA SILVA, J. C.; FERNANDES, R. P.; LOUZEIRO, H. C.; DE PAULA, M. L.; MOUCHREK, V. E.; Fontenele, M. A. Larvicidal activity, molluscicide and toxicity of the essential oil of Citrus limon peels against, respectively, Aedes aegypti, Biomphalaria glabrata and Artemia salina. Eclética Química Journal, v. 44, n. 4, p. 85-95, 2019.

GOVINDARAJAN, M.; SIVAKUMAR, R.; RAJESWARI, M.; YOGALAKSHMI, K. Chemical composition and larvicidal activity of essential oil from Mentha spicata (Linn.) against three mosquito species. Parasitology research, v. 110, n. 5, p. 2023-2032, 2012.

ISMAN, M. B. Pesticides based on plant essential oils: phytochemical and practical considerations. In: Medicinal and Aromatic Crops: Production, Phytochemistry, and Utilization. American Chemical Society, 2016. p. 13-26.

KAKARAPARTHI, P. S.; SRINIVAS, K. V. N. S.; KUMAR, J. K.; KUMAR, A. N.; RAJPUT, D. K.; SARMA, V. U. M. Variation in the essential oil content and composition of Citronella (Cymbopogon winterianus Jowitt.) in relation to time of harvest and weather conditions. Industrial Crops and Products, v. 61, p. 240-248, 2014.

LEE, HS. Mosquito larvicidal activity of aromatic medicinal plant oils against Aedes aegypti and Culex pipiens pallens. Journal of the American Mosquito Control Association, v. 22, n. 2, p. 292-295, 2006.

LEITE, B. L.; SOUZA, T. T.; ANTONIOLLI, A. R.; GUIMARÃEE, A. G.; SIQUEIRA, R. S.; QUINTANS, J. S.; BONJARDIM, L. R.; ALVES, P. B.; BLANK, A. F.; BOTELHO, M. A.; ALMEIDA, J. R. G. S.; LIMA, J. T.; ARAÚJO, A. A. S.; QUINTANS-JUNIOR, L. J. Volatile constituents and behavioral change induced by Cymbopogon winterianus leaf essential oil in rodents. African Journal of Biotechnology, v. 10, n. 42, p. 8312-8319, 2011.

LIMA-CAMARA, T. N. Arboviroses emergentes e novos desafios para a saúde pública no Brasil. Revista de Saúde Pública, v. 50, p. 36, 2016.

MACEDO, I. T.; BEVILAQUA, C. M.; OLIVEIRA, L. M.; CAMURÇA-VASCONCELOS, A. L.; VIEIRA, L. D. S.; OLIVEIRA, F. R.; QUEIROZ-JÚNIOR, E. M.; TOMÉ, A. R.; NASCIMENTO, N. R. Anthelmintic effect of Eucalyptus staigeriana essential oil against goat gastrointestinal nematodes. Veterinary Parasitology, v. 173, n. 1–2, p. 93–98, 2010.

MANZOOR, F.; SAMREEN, K. B.; PARVEEN, Z. Larvicidal activity of essential oils against Aedes aegypti and Culex quinquefasciatus larvae (Diptera: Culicidae). J. Anim. Plant Sci, v. 23, n. 2, p. 420-424, 2013.

MENDONÇA, F. A.; SILVA, K. F. S.; SANTOS, K. K.; RIBEIRO JÚNIOR, K. A. L.; SANT'ANA, A. E. G. Activities of some Brazilian plants against larvae of the mosquito Aedes aegypti. Fitoterapia, v. 76, n. 7-8, p. 629-636, 2005.

MEYER, B. N.; FERRIGNI, N. R.; PUTNAM, J. E.; JACOBSEN, L. B.; NICHOLS, D. J.; MCLAUGHLIN , J. L. Brine shrimp: a convenient general bioassay for active plant constituents. Planta medica, v. 45, n. 05, p. 31-34, 1982.

MORAIS, S. M.; CAVALCANTI, E. S.; BERTINI, L. M.; BERTINI, C. L. L.; RODRIGUES, J. R. B.; CARDOSO, J. H. L. Larvicidal activity of essential oils from Brazilian Croton species against Aedes aegypti L. Journal of the American Mosquito Control Association, v. 22, n. 1, p. 161-164, 2006.

PEREIRA, G. P.; PRADO, A. E.; CARVALHO, R. I. N.. Variação mensal do rendimento de óleo essencial de citronela. Revista Eletrônica Científica da UERGS, v. 2, p. 183-189, 2016.

PERTIWI, G. P. Pengaruh Lama Waktu Penyimpanan dan Penyinaran Cahaya Terhadap Sitronelal serta Uji Toksisitas Menggunakan Metode BSLT (Brine Shrimp Lethality Test). 2014. Tese de Doutorado. Universitas Brawijaya.

PHASOMKUSOLSIL, S.; SOONWERA, M. Potential larvicidal and pupacidal Activities of herbal essential oils against Culex quinque fasciatus Say and Anopheles minimus (Theobald). Southeast Asian J Trop Med Public Health, v.41, n.6, p.1342 – 1351, 2010.

PITASAWAT, B.; CHAMPAKAEW, D.; CHOOCOTE, W.; JITPAKDI, A.; CHAITHONG, U.; KANJANAPOTHI, D.; RATTANACHANPICHAI, E.; TIPPAWANGKOSOL, P.; RIYONG, D.; TUETUN, B.; CHAIYASIT, D. Aromatic plant-derived essential oil: an alternative larvicide for mosquito control. Fitoterapia, v. 78, n. 3, p. 205-210, 2007.

PUSHPANATHAN, T.; JEBANESAN, A.; GOVINDARAJAN, M. Larvicidal, ovicidal and repellent activities of Cymbopogan citratus Stapf (Graminae) essential oil against the filarial mosquito Culex quinquefasciatus (Say)(Diptera: Culicidae). Trop Biomed, v. 23, n. 2, p.p 208-212, 2006.

ROCHA, S.; MING, L. C.; MARQUES, M. Influência de cinco temperaturas de secagem no rendimento e composição do óleo essencial de citronela (Cymbopogon winterianus Jowitt). Revista Brasileira de Plantas Medicinais, p. 73-78, 2000.

RODRIGUES, K. A. D. F.; DIAS, C. N.; AMARAL, F. M. M.; MORAES, D. F.; MOUCHREK FILHO, V. E.; ANDRADE, E. H. A.; MAIA, J. G. S. Molluscicidal and larvicidal activities and essential oil composition of Cymbopogon winterianus. Pharmaceutical biology, v. 51, n. 10, p. 1293-1297, 2013.

SINHA, S.; JOTHIRAMAJAYAM, M.; GHOSH, M.; MUKHERJEE, A. Evaluation of toxicity of essential oils palmarosa, citronella, lemongrass and vetiver in human lymphocytes. Food and Chemical Toxicology, v. 68, p. 71-77, 2014.

TABANCA, N.; ÖZEK, G.; ALI, A.; DURAN, A.; HAMZAOGLU, E.; BASER, K. H. C.; KHAN, I. A. Chemical composition of Heracleum pastinacifolium subsp. transcaucasicum and subsp. incanum essential oils, and their biting deterrent and larvicidal activity against Aedes aegypti. Planta Medica, v. 78, n. 05, p. P_89, 2012.

TAWATSIN, A.; WRATTEN, S. D.; SCOTT, R. R.; THAVARA, U.; TECHADAMRONGSIN, Y. Repellency of volatile oils from plants against three mosquito vectors. Journal of vector ecology, v. 26, p. 76-82, 2001.

VELETOVAC, A. Toxic essential oils. Tese (Doutorado em Farmácia) – Universität Wien. Viena, p 59. 2016.

VELOSO, R. A.; CASTRO, H. G.; CARDOSO, D. P.; CHAGAS, L. F. B.; FREITAS, A.; JÚNIOR, C. Óleos essenciais de manjericão e capim citronela no controle de larvas de Aedes aegypti. Revista Verde (Pombal-PB-Brasil) v, v. 10, n. 2, p. 101-105, 2015.

WHO. World Health Organization Technical Reports Series n0 443, 1970.

WOLFFENBUTTEL, A. N. Óleos essenciais. Informativo CRQ-V, ano XI, n. º105, págs, v. 6, 2007.

ZARA, A. L. D. S. A.; SANTOS, S. M. D.; FERNANDES-OLIVEIRA, E. S.; CARVALHO, R. G.; COELHO, G. E. Estratégias de controle do Aedes aegypti: uma revisão. Epidemiologia e Serviços de Saúde, v. 25, p. 391-404, 2016.




DOI: https://doi.org/10.5902/2179460X44044

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