GENETIC DIVERSITY AND POPULATION STRUCTURE OF JATOBÁ: A SPECIES WITH ECONOMIC POTENTIAL FOR THE AMAZON REGION
DOI:
https://doi.org/10.5902/1980509832033Keywords:
Hymenaea courbaril, genetic variability, ISSR, conservationAbstract
Jatobá (Hymenaea courbaril L.) is a species that shows ecological importance and occurs in different geographic regions in Brazil. Knowing and evaluating the existence of places with higher genetic variability can help in conservation programs and actions. In this context, the present study aimed to evaluate the genetic diversity and structure in natural populations of Jatobá with occurrence in Mato-Grosso Amazon through ISSR markers. Fifty-four individuals were sampled in three populations, being one in the municipality of Marcelândia (MA=24) and two in the municipality of Alta Floresta (Comunidade Central AF=17 and Pista do Cabeça PC=13 individuals). Total genomic DNA was extracted from the leaf tissue by the CTAB method. The 54 individuals were genotyped with 10 ISSR primers. A total of 110 fragments were amplified, being 78.2% polymorphic. The greatest diversity indices were found in the population from MA (H=0.25; I=0.37 and %P=65.45). The genetic distance was higher among the populations of the two municipalities (0.17 between MA and AF; 0.16 between MA and PC). The analysis of molecular variance (AMOVA) indicated that 31.79 % of the total variance is among populations and 68.21 % within populations. There is genetic diversity in native populations of Jatobá in Mato-Grosso Amazon. We recommend that individuals from both populations are preserved, in order to ensure the maintenance of genetic variability and the effective conservation of species in Mato-Grosso Amazon.
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References
ALVES, R. M. et al. High levels of genetic divergence and inbreeding in populations of cupuassu (Theobroma grandiflorum). Tree Genetics & Genomes, Heidelberg, v. 3, n. 4, p. 289-298, oct. 2007.
BAWA, K. S. Breeding systems of tree species of a lowland tropical community. Evolution, New York, v. 28, n. 1, p. 85-92, mar. 1974.
BOTSTEIN, D. et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American journal of human genetics, Chicago, v. 32, n. 3, p. 314-331, may 1980.
BUSATTO, P. C. et al. Superação de dormência em sementes de jatobá (Hymenaea courbaril L.). Revista Verde de Agroecologia e Desenvolvimento Sustentável, Mossoró, v. 8, n. 1, p. 154-160, mar. 2013.
CARVALHO, P. E. R. et al. Espécies florestais brasileiras: recomendações silviculturais, potencialidades e uso da madeira. Brasília: EMBRAPA; CNPF; SPI, 1994.
CATAUDELLA, R. et al. Genetic variability of Austropotamobius italicus in the Marches region: implications for conservation. Aquatic Conservation: Marine and freshwater ecosystems, Nova Jersey, v. 20, n. 3, p. 261-268, mar. 2010.
CRUZ, C. D. Programa GENES: aplicativo computacional em genética e estatística. Viçosa, MG: UFV, 2008.
DOYLE, J. J.; DOYLE, J. L. A rapid DNA isolation procedure for small amounts of fresh leaf tissue. Phytochemical Bulletin, California, v. 19, p. 11-15, 1987.
ESTOPA, R. A. et al. Diversidade genética em populações naturais de candeia (Eremanthus erythropappus (DC.) MacLeish). Scientia Forestalis, Piracicaba, n. 70, p. 97-106, abr. 2006.
EVANNO, G.; REGNAUT, S.; GOUDET, J. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular ecology, Oxford, v. 14, n. 8, p. 2611-2620, fev. 2005.
EXCOFFIER, L.; LAVAL, G.; SCHNEIDER, S. An integrated software package for population genetics data analysis, version 3.01. Switzerland: University of Berne, 2006.
EXCOFFIER, L.; SMOUSE, P. E.; QUATTRO, J. M. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics, Bethesda, v. 131, n. 2, p. 479-491, jun. 1992.
GIBBS, P. E.; OLIVEIRA, P. E.; BIANCHI, M. B. Postzygotic control of selfing in Hymenaea stigonocarpa (Leguminosae‐Caesalpinioideae), a bat‐pollinated tree of the Brazilian cerrados. International Journal of Plant Sciences, Chicago, v. 160, n. 1, p. 72-78, jan. 1999.
GIUSTINA, L. D. et al. Population structure and genetic diversity in natural populations of Theobroma speciosum Willd. Ex Spreng (Malvaceae). Genetics and Molecular Research, Ribeirão Preto, v. 13, n. 2, p. 47-53, fev. 2014.
LEWIS, G. et al. Legumes of the world. Kew: Royal Botanic Gardens, 2005.
LIMA, A. L. D. S.; ZANELLA, F.; CASTRO, L. D. M. D. Growth of Hymenaea courbaril L. var. stilbocarpa (Hayne) Lee et Lang. e Enterolobium contortisiliquum (Vell.) Morong (Leguminosae) under different shading levels. Acta Amazonica, Manaus, v. 40, n. 1, p. 43-48, mar. 2010.
MATO GROSSO. Secretaria de Estado de Planejamento e Coordenação Geral. Unidades Climáticas do Estado de Mato Grosso. Cuiabá: PRODEAGRO, 2006. CD-Rom.
MELO, M. G. G.; MENDES, A. M. S. Jatobá (Hymenaea courbaril L.). Manaus: Rede de sementes da Amazônia, 2005. 2 p. (Informativo Técnico, 9).
NEI, M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, Bethesda, v. 89, n. 3, p. 583-590, jul. 1978.
PAIVA, J. R. Melhoramento genético de espécies agroindustriais na Amazônia: estratégias e novas abordagens. Brasília: Embrapa SPI; Fortaleza: Embrapa CNPAT, 1998.
PRITCHARD, J. K.; STEPHENS, M.; DONNELLY, P. Inference of population structure using multilocus genotype data. Genetics, Bethesda, v. 155, n. 2, p. 945-959, jun. 2000.
PRITCHARD, J. K.; WEN, W. Documentation for structure software: version 2.1. [s. l.: s. n.], 2004. Disponível em: <http://pritch.bsd.uchicago.edu>. Acesso em: 20 jan. 2016.
RIVAS, L. H. et al. Genetic diversity in natural populations of Theobroma subincanum Mart. in the Brazilian Amazon. Genetics and Molecular Research, Ribeirão Preto, v. 12, n. 4, p. 4998-5006, out. 2013.
ROSA, R. D.; PERIN, C. L.; ROSA, R. D. Colonizador e colonos: na fronteira da terra o limite dos sonhos de um futuro promissor. Revista do Programa de Ciências Agro-Ambientais, Alta Floresta, v. 2, n. 1, p. 71-82, 2003.
ROSSI, F. S. et al. Diversidade genética em populações naturais de Mauritia flexuosa L. f. (Arecaceae) com uso de marcadores ISSR. Scientia Forestalis, Piracicaba, v. 42, n. 104, p. 631-639, dez. 2014.
SANTANA, I. B. B. et al. Variabilidade genética entre acessos de umbu-cajazeira mediante análise de marcadores ISSR. Revista Brasileira de Fruticultura, Jaboticabal, v. 33, n. 3, p. 868-876, fev. 2011.
SMOUSE, P. E.; SORK, V. L. Measuring pollen flow in forest trees: an exposition of alternative approaches. Forest Ecology and Management, Amsterdam, v. 197, n. 1, p. 21-38, aug. 2004.
YEH, F. C.; YANG, R.; BOYLE, T. POPGENE. Microsoft Windows-based freeware for population genetic analysis. Release 1.31. Edmonton: University of Alberta, 1999.
