Vegetative rescue, <i>in vitro</i> establishment and cutting technique of <i>Drimys brasiliensis</i> Miers
DOI:
https://doi.org/10.5902/1980509869093Keywords:
Vegetative propagation, Rejuvenation, In vitro cultivation, Native speciesAbstract
Drimys brasiliensis Miers, popularly known as cataia, is a tree species native to the Atlantic Forest that has phytochemical, phytotherapeutic, aromatic and economic importance, with wide use in folk medicine. It is considered a species of difficult multiplication via seeds, presenting dormancy due to embryonic immaturity. Thus, the objective of this study was to analyze the vegetative rescue, in vitro establishment and propagation by way of cuttings of Drimys brasiliensis. For the rescue, the girdling techniques were applied at 30 and 90 cm from the ground, semi-girdling at 30 cm from the ground and pruned branches conditioned vertically and horizontally, being evaluated the shoot emission capacity. With the shoots obtained by vegetative rescue, the explants used for in vitro establishment were made, evaluating different immersion times in sodium hypochlorite 1.0 % (v/v) (0, 10, 15 and 20 minutes). The percentages of total, bacterial, fungal contamination and phenolic oxidation were evaluated. On the cuttings, shoots from vegetative rescue, from the aerial part of an adult individual and from detached branches were tested and placed in a mini-tunnel. The percentage of cuttings survival, percentage of cuttings with callus and percentage of rooting were evaluated. Regarding the vegetative rescue, the technique of pruned branches resulted in the induction of shoots, highlighting the branches conditioned vertically (100%). In the in vitro establishment, the immersion in sodium hypochlorite (1%) for 20 minutes proved to be efficient in the control of fungal contamination, however, no significant differences were observed in relation to the control of bacterial contamination. In cuttings, the use of cuttings from pruned branches or girdling/semi-girdling showed potential for these techniques in the cloning of the species, obtaining results close to 10% rooting. In general, the species has the potential for vegetative propagation, however, adjustments are necessary in the in vitro establishment and cutting protocols.
Downloads
References
ABREU, D. C. A. et al. Caracterização morfológica de frutos e sementes de cataia (Drimys in vitr Miers. - Winteraceae). Revista Brasileira de Sementes, Londrina, v. 27, n. 2, p. 67-74, 2005.
AGUIAR, N. S. et al. Ácido indolbutírico na estaquia de Calliandra brevipes e Calliandra tweedii. Advances in Forestry Science, Cuiabá, v. 8, n. 1, p. 1327-1333, 2021.
ALMEIDA, F. D. et al. Vegetative propagation of selected Eucalyptus cloeziana F. Muell. trees through cutting technique. Revista Árvore, Viçosa, v. 31, n. 3, p. 445-453, 2007.
ALVARES, C. A. et al. Köppen’s climate classification map for Brazil. Meteorologische Zeitschrift, Stuttgart, v. 22, n. 6, p. 711-728, 2013.
BACKES, A.; NARDINO, M. Árvores, arbustos e algumas lianas nativas no Rio Grande do Sul. São Leopoldo: UNISINOS, 1998, 202 p.
BADILLA, Y. et al. Eficiência do AIB no enraizamento de miniestacas de clones de Teca (Tectona grandis Linn F.). Revista Árvore, Viçosa, v. 40, n. 3, p. 477-485, 2016.
BARRUETO CID, L. P.; ZIMMERMANN, M. J. A. Contaminação in vitro de plantas. Boletim de Pesquisa e Desenvolvimento, Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, p. 20, 2006.
BITENCOURT, J. et al. Enraizamento de estacas de erva-mate (Ilex paraguariensis St. Hill) provenientes de brotações rejuvenescidas. Revista Brasileira de Plantas Medicinais, Botucatu, v. 11, p. 277-281, 2009.
CARVALHO, G. C. M. W. Resgate de matrizes e propagação vegetativa de Pathymenia reticulata Benth. Dissertação (Mestrado) – Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, 2020.
DIAS, P. C. et al. Vegetative rescue of Anadenanthera macrocarpa trees. Cerne, Lavras, v. 21, n. 1, p. 83-89, 2015.
ERIG, A. C.; SCHUCH, M. W.; Tipo de explante e controle da contaminação e oxidação no estabelecimento in vitro de plantas de macieira (Malus domestica Borkh.) cvs. Galaxy, Maxigala e Mastergala. Revista Brasileira Agrociência, Pelotas, v. 9, p. 221-227, 2005.
FERREIRA, M. G. R. et al. Propagação in vitro de cupuaçuzeiro. Saber Científico, Porto Velho, v. 2, n. 2, p. 37-44, 2021.
FERREIRA, D. F. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia, Lavras, v. 38, n. 2, p. 109-112, 2014.
FERRIANI, A. P. et al. Sprouts production and rooting of Piptocarpha angustifolia minicuttings. Pesquisa Florestal Brasileira, Colombo, v. 31, n. 67, p. 257-264, 2011.
FOLADORI-INVERNIZZI, S. et al. Estado da arte da propagação vegetativa por estaquia de espécies arbustivo-arbóreas. Revista Eletrônica Científica da UERGS, Porto Alegre, v. 7, n. 1, p. 50-63, 2021.
GEORGE, E. F. Plant propagation by tissue culture - Part 1: The technology. Westbury: Exectics, 1993. 574p.
HARTMANN, H. T. et al. Plant propagation: principles e practices. 8. ed. Boston: Prentice Hall, p. 915, 2011.
HUSSAIN, I. et al. Cutting types collected at different seasons on Blackberry multiplication. Revista Brasileira de Fruticultura, Jaboticabal, v. 39, n. 3, 2017.
SANTOS JUNIOR, C. F. S. et al. Resgate vegetativo de Cedrela fissilis Vell. por enraizamento de estacas de brotos epicórmicos e de copa. Ciência Rural, Santa Maria, v. 51, n. 8, p. 6, 2021.
LUZ, J. M. Q. et al. Estabelecimento in vitro e aclimatização de Lippia alba (Mill.) NE Brown. Revista Brasileira de Plantas Medicinais, Botucatu, v. 16, p. 444-449, 2014.
MARCHIORI, J. N. C. Dendrologia das angiospermas: das magnoliáceas às acurtiáceas. Santa Maria: UFSM, p. 271, 1997.
MENEGUZZI, A. Resgate vegetativo e propagação in vitro de Persea willdenovii Kosterm. Dissertação (Mestrado) - Universidade do Estado de Santa Catarina, Lages, 2017.
MURASHIGE, T., SKOOG, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, Copenhagen, v. 15, p. 473-497, 1962.
NADHA, H. K. et al. Identification and elimination of bacterial contamination during in vitro propagation of Guadua angustifolia Kunth. Pharmacognosy Magazine, Bangalore, v. 8, n. 30, p. 93-97, 2012.
OLIVEIRA, N. P. et al. Controle de contaminação e oxidação no cultivo in vitro de oliveira (Olea europaea L.) cv. “Koroneiki”. Research, Society and Development, Vargem Grande Paulista, v. 10, n. 5, p. e30710514929-e30710514929, 2021.
PORFÍRIO, S. et al. Reviewing current knowledge on olive (Olea europaea L.) adventitious root formation. Scientia Horticulturae, v. 198, p. 207-226, 2016.
SIMÕES, C. M. O. et al. Plantas da medicina popular no Rio Grande do Sul. Porto Alegre: Ed. da Universidade/ UFRGS, p. 147, 1986.
SOUZA, J. C. A. V. et al. Propagação vegetativa de cedro australiano (Toona ciliata M. Roemer) por miniestaquia. Revista Árvore, Viçosa, v.33, p.205-213, 2009.
STUEPP, C. A. et al. Vegetative propagation and application of clonal forestry in Brazilian native tree species. Pesquisa Agropecuária Brasileira, Brasília, v.53, p. 985-1002, 2018.
TEIXEIRA, J. B. Limitações ao processo de cultivo in vitro de espécies lenhosas. Brasilia: Embrapa – Recursos. Genéticos e Biotecnologia Brasília, 2005.
TOSTA, M. S. et al. Ácido indolbutírico na propagação vegetativa de cajaraneira. (Spondias sp). Semina: Ciências Agrárias, Londrina, v. 33, p. 2727-2740, 2012.
WENDLING, I. et al. Maturation in Corymbia torelliana × C. citriodora stock plants: effects of pruning height on shoot production, adventitious rooting capacity, stem anatomy, and auxin and abscisic acid concentrations. Forests, v. 6, p. 3763-3778, 2015.
WENDLING, I; BRONDANI, G. Vegetative rescue and cuttings propagation of Araucaria angustifolia (Bertol.) Kuntze. Revista Árvore, Viçosa, v. 39, p. 93-104, 2015.
WENDLING, I. et al. Maturation and related aspects in clonal forestry – Part II: reinvigoration, rejuvenation and juvenility maintenance. New Forests, v. 45, n. 4, p. 473-486, 2014.
WENDLING, I. et al. Vegetative propagation of adult Ilex paraguariensis trees through epicormic shoots. Acta Scientiarum, Maringá, v. 35, n. 1, p. 117-125, 2013.
WENDLING, I.; XAVIER, A. Gradiente de maturação e rejuvenescimento aplicado em espécies florestais. Floresta e Ambiente, Seropédica, v. 8, p. 187-194, 2012.
WENDLING, I. et al. Indução de brotações epicórmicas ortotrópicas para a propagação vegetativa de árvores adultas de Araucaria angustifolia. Agronomía Costarricense, San José, v. 33, n. 2, p. 309-319, 2009.
XAVIER A. et al. Silvicultura clonal: princípios e técnicas. 2. ed. Viçosa, MG: Ed. da UFV, 2013. 279 p.
YOSHIKO, A. S.; TEIXEIRA, H. C. D. Micropropagação de Celtis sp: controle da contaminação e oxidação. Cerne, Lavras, v. 7, n. 2, p. 117-123, 2001.
ZHANG, W. et al. Mechanisms underlying the regulation of root formation in Malus hupehensis stem cuttings by using exogenous hormones. Journal Plant Growth Regulation, v. 36, n. 1, p. 1-12, 2016.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Ciência Florestal
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
A revista CIÊNCIA FLORESTAL reserva-se o direito de realizar, nos originais, alterações de ordens normativas, ortográficas e gramaticais, com vistas a manter o padrão escolar da língua, mas respeitando o estilo dos autores. As provas finais podem ou não ser enviadas aos autores.
