Larvicidal activity of essential oils and nanoemulsions (o/w) from Eucalyptus globulus Labil and Ocimum africanum Lour
DOI:
https://doi.org/10.5902/2179460X73704Keywords:
Eucalyptus globulus, Ocimum africanum, LarvicidalAbstract
The study aimed to evaluate the chemical profile and larvicidal activity of essential oil (EO) and oil-in-water (O/W) nanoemulsions from the leaves of Eucalyptus globulus Labil and Ocimum africanum Lour. The plant material was collected in the city of São Luís-MA. For the extraction of essential oils, the hydrodistillation technique was used. The determination of total phenolic compounds was performed by the Folin-Ciocalteu spectrophotometric method. Antioxidant activity was performed by the spectrophotometric method of scavenging hydroxyl radicals. Toxicity was measured using the Artemia salina Leach bioassay, and the Lethal concentration 50% (LC50) for each of the essential oils was calculated based on the Reed&Muench method. For larvicidal activity, Aedes aegypti larvae were submitted to the EO solution at concentrations of 10-100 mg L-1, where the mortality of the larvae was evaluated and the Lethal concentration of 50% (LC50) was determined. The larvicidal action of the EO of E. globulus and O. africanum was observed, where LC50 of 26.58 mg L-1 and LC50 of 35.89 mg L-1, respectively, and their nanoemulsions with LC50 of 12. 01 mg L-1 and 14.75 mg L-1. Through the results obtained, it was proven that the larvicidal and improved action of the nanoemulsions in the tests carried out. Finally, this study points to its efficient larvicidal activity against Aedes aegypti larvae, encouraging its application in arbovirus vector control areas.
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