Effect of light quality <i>in vitro</i> multiplication and rooting of <i>Cedrela odorata</i> L.
DOI:
https://doi.org/10.5902/1980509866513Keywords:
Vegetative propagation, Micropropagation, LEDsAbstract
The light source can regulate the morphogenesis of the in vitro culture. The objective of this work was to evaluate the effect of four different LED light: white, red, blue and pink (50% blue and 50% red) under the stages of multiplication and rooting of in vitro cultures of Cedrela odorata. In the multiplication phase, the MS culture medium was added to 8.8 μM of 6-benzylaminopurine (BAP) and 0.28 μM of gibberellic acid (GA3) under the effect of the types of light. Rooting was evaluated the interaction of light types under two concentrations of indolebutyric acid IBA (4.9 and 9.8 μM). In multiplication, under white LED light, 3.9 sprouts per explant were obtained, with longer sprouts (2.1 mm) and no oxidation. Similarly, at rooting, cultures under white LED light were higher in the number of roots formed and lower oxidation. It was found that white LED light optimized the multiplication and rooting phase in the micropropagation of this forest species according to this study.
Downloads
References
ALMEIDA, L. V. S.; OLIVEIRA, V. J. S. O.; JACOBI, C. C. B., ALMEIDA, W. A. B., CARVALHO, M. J. S. Vernonia condensata Baker: an alternative for large-scale seedling production. Ciência Rural, v. 50, n. 3, e20180941, 2020.
AMARAL, V. F. M. Multiplicação in vitro: Cedrela fissilis Vell., 2006, 60 f. Dissertação (Mestrado em Engenharia Floresta), Universidade Federal de Santa Maria, Santa Maria/RS, 2006.
AZMI, N. S.; AHMAD, R.; IBRAHIM, R. Fluorescent light (FL), red LED and blue LED spectrums effects on in vitro shoots multiplication. Jurnal Teknologi, v. 78, n. 6-6, p. 93-97, 2016.
BASSAN, J. S.; REINIGER, L. R. S.; ROCHA, B. H. G.; SEVERO, C. R. P.; FLÔRES, A. V. Oxidação fenólica, tipo de explante e meios de cultura no estabelecimento in vitro de canafístula (Peltophorum dubium (SPRENG.) TAUB.). Ciência Florestal, v. 16, n. 4, p. 381-390, 2006.
BELLO-BELLO, J. J.; MARTÍNEZ-ESTRADA, E.; CAAMAL-VELÁZQUEZ, J. H.; MORALES-RAMOS, V. Effect of LED light quality on in vitro shoot proliferation and growth of vanilla (Vanilla planifolia Andrews). African Journal of Biotechnology, v. 15, n. 8, p. 272-277, 2016.
BORGES, L. P.; AMORIM, V. A. Metabólitos secundários de plantas. Revista Agrotecnologia, v. 11, n. 1, p. 54-67, 2020.
BRASIL. Ministério do Meio Ambiente. Portaria nº 444 de 17 de dezembro de 2014 - Anexo I. Lista nacional oficial de espécies da fauna ameaçadas de extinção. 2014. Diário Oficial da União da República Federativa do Brasil, Brasília, DF, seção 1, 18 de dezembro de 2014.
CIOĆ, M.; SZEWCZYK, A., ŻUPNIK, M.; KALISZ, A.; PAWŁOWSKA, B. LED lighting affects plant growth, morphogenesis, and phytochemical contents of Myrtus communis L. in vitro. Plant Cell Tissue and Organ Culture, v. 132, n. 3, p. 433-447, 2018.
DEBERGH, C. P.; MAENE, L. J. A scheme for commercial propagation of ornamental plants by tissue culture. Scientia Horticulturae, v. 14, n. 4, p. 335-345, 1981.
FERRARI, M. P.; GROSSI, F.; WENDLING, I. Propagação vegetal de espécies florestais, Colombo - Paraná: Embrapa Florestal (documento, 94), 2004. 22 p.
GARCÍA-GONZÁLES, R.; DELGADO, M.; GONZÁLEZ, Y.; GONZÁLEZ, A.; GARRIGA, M.; CALIGARI, P. D. S.; CARRASCO, B.; QUIROZ, K. In vitro propagation of cedar (Cedrela odorata L.) from juvenile shoots. Chilean Journal of Agricultural Research, v. 71, n. 3, p. 376-382, 2011.
HUNG, C. D.; HONG, C. H.; JUNG, H. B.; KIM, S. K.; KET, N. V.; NAM, M. W., CHOI, D. H.; LEE, H. I. Growth and morphogenesis of encapsulated strawberry shoot tips under mixed LEDs. Scientia Horticulturae, v. 194, p. 194-200, 2015.
KESSIN, J. P.; PIGOZZI, L.; HAHN, P. C.; DIAS, G. V.; NUNES, M. R.; DALLA-COSTA, M.; VEECK, A. P. L. Atividade antioxidante de compostos fenólicos presentes em polpa e casca de goiabeira serrana. Brazilian Journal of Food Research, v. 9, n. 1, p. 141-153, 2018.
KOBUS-CISOWSKA, J.; SZCZEPANIAK, O.; SZYMANOWSKA-POWAŁOWSKA, D.; PIECHOCKA, J.; SZULC, P.; DZIEDZIŃSKI, M. Antioxidant potential of various solvent extract from Morus alba fruits and its major polyphenols composition. Ciência Rural, v. 50, n. 1, e20190371, 2019.
LAZZARINI, L. E. S.; PACHECO, F. V.; SILVA, S. T.; COELHO, A. D.; MEDEIROS, A. P. R.; BERTOLUCCI, S. K. V.; PINTO, J. E. B. P.; SOARES, J. D. R. Uso de diodos emissores de luz (LED) na fisiologia de plantas cultivadas - revisão. Scientia Agraria Paranaensis, v. 16, n. 2, p. 137-144, 2017.
LESHER-GORDILLO, J. M.; MARTÍNEZ-SÁNCHEZ, J. L.; ORDUÑA-RODAS, P.; HERNÁNDEZ-CARRETA, A. I. Genetic diversity of the red cedar (Cedrela odorata) in the state of Tabasco, México. Bosque, v. 39, n. 3, p. 411-417. 2018.
MANTOVANI, N. C.; FRANCO, E. T. H.; VESTENA, S. Regeneração in vitro de louro-pardo (Cordia trichotoma (Vellozo) Arrabida ex Steudel). Ciência Florestal, v. 11, n. 2, p. 93-101, 2001.
MURASHIGE, T. Plant propagation throught tissue culture. Annual Review Plant Physiology, v. 25, p. 135-166, 1974.
MURASHIGE, T.; SKOOG, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, v.15, p.473-497, 1962.
NUNES, E. C.; CASTILHO, C. V.; MORENO, F. N.; VIANA, A. M. In vitro culture of Cedrela fissilis Vellozo (Meliaceae). Plant Cell, Tissue and Organ Culture, v. 70, p. 259-268, 2002.
PEÑA-RAMÍREZ, Y. J.; JUÁREZ-GÓMEZ, J.; GÓMEZ-LÓPES, L.; JERÓNIMO-PÉREZ, J. L.; GARCÍA-SHESEÑA, I.; GONZÁLEZ-RODRÍGUEZ, J. A.; ROBERT, M. L. Multiple adventitious shoot formation in spanish red cedar (Cedrela odorata L.) cultured in vitro using juvenile and mature tissues: an improved micropropagation protocol for a highly valuable tropical tree species. In Vitro Cellular & Developmental Biology - Plant, v. 46, n. 2, p. 49-160, 2010.
PINHEIRO, M. V. M.; SCHMIDT, D.; DIEL, M. I.; SANTOS, J.; THIESEN, L. A.; AZEVEDO, G. C. V.; HOLZ, E. In vitro propagation of alpinia cultivars in different light sources. Ornamental Horticulture, v. 25, n. 1, p. 49-54, 2019.
R CORE TEAM. R: A language and environment for statistical computing. Vienna: R Foundation for Statistical Computing, 2018. Disponível em: https://www.R-project.org/. Acesso em: 10 jan. 2021.
RODRÍGUEZ, R.; DAQUINTA, M.; CAPOTA, I.; PINA, D.; LEZCANO, Y.; GONZÁLEZ-OLMEDO, J. L. Nuevos aportes a la micropropagación de Swietenia macrophylla × Swietenia mahogani (Caoba híbrida) y Cedrela odorata (Cedro). Cultivos Tropicales, v. 24, n. 3, p. 23-27, 2003.
SALLES, E. A. P. B.; ALCANTARA, G. B.; QUOIRIN, M. G. G.; GONÇALVES, A. N.; HIGA, A. R. Desinfestação e introdução in vitro de segmentos nodais de Acacia mearnsii. Pesquisa Florestal Brasileira, v. 37, n. 92, p. 485-491, 2017.
SANTOS JUNIOR, C. F.; DALLA-COSTA, M.; RECH, T. D.; BOFF, P.; BOFF, M. I. C. Use of micropropagation in the vegetative rescue of adult trees of Cedrela odorata L. Revista Brasileira de Ciências Agrárias, v. 16, n. 4, e8944, 2021.
SAYED, S. S.; GABR, A. M. M.; AMIN, M. A.; TAHA, L. S. Biochemical characterization of micropropagated Ceratonia siliqua L. under effect of growth regulators and light quality. Bulletin of the National Research Centre, v. 44, n. 26, 2020.
SOUZA, D. M. S. C.; FERNANDES, S. B.; AVELAR, M. L. M.; FRADE, S. R. P.; MOLINARI, L. V.; GONÇALVES, D. S.; PINTO, J. E. B. P.; BRONDANI, G. E. Light quality in micropropagation of Eucalyptus grandis × Eucalyptus urophylla. Scientia Forestalis, v. 48, n. 127, e3329, 2020.
SOUZA, D. M. S. C.; XAVIER, A.; OTONI, W. C.; MIRANDA, N. A.; MAGGIONI, J. H. Light quality in the in vitro introduction of Corymbia hybrid clones. Revista Árvore, v. 42, n. 6, e420604, 2018.
STUEPP, C. A.; WENDLING, I.; XAVIER, A.; ZUFFELLATO-RIBAS, K. C. Vegetative propagation and application of clonal forestry in Brazilian native tree species. Pesquisa Agropecuária Brasileira, Brasília, v. 53, n. 9, p. 985-1002, 2018.
TAIZ, L.; ZEIGER, E. Fisiologia vegetal. 4. ed. Porto Alegre: Artmed, 2009. 848 p.
VALVERDE-CERDAS, L.; ROJAS-VARGAS, A.; HINE-GÓMEZ, A. In vitro propagation of Albizia guachapele, Cedrela odorata, Platymiscium pinnatum and Guaiacum sanctum. Plant Tissue Culture and Biotechnology, v. 18, n. 2, p. 151-156, 2008.
ZHANG, Z.; SUN, Y.; LI, Y. Plant rejuvenation: from phenotypes to mechanisms. Plant Cell Reports, v. 39, n. 10, p. 1249-1262, 2020.
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Ciência Florestal
![Creative Commons License](http://i.creativecommons.org/l/by-nc/4.0/88x31.png)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
A revista CIÊNCIA FLORESTAL reserva-se o direito de realizar, nos originais, alterações de ordens normativas, ortográficas e gramaticais, com vistas a manter o padrão escolar da língua, mas respeitando o estilo dos autores. As provas finais podem ou não ser enviadas aos autores.