Analysis of cuticular chemical profiles of Latrodectus geometricus (Araneae: Theridiidae) females and juveniles using GC×GC/qMS
DOI:
https://doi.org/10.5902/2179460X29908Keywords:
Gas chromatography, Spider, Communication, Mass spectrometryAbstract
Communication in spiders can occur by several mechanisms, such as chemical cues and the cuticular hydrocarbons (CHCs) play an important role in intraspecific recognition. Several techniques have been used to evaluate CHCs in spiders, such as Gas Chromatography with Flame Ionization Detection, Gas Chromatography coupled with Mass Spectrometric detection and recently Fourier Transformed Infrared Photoacoustic Spectroscopy. In this study, rapid-scanning two-dimensional gas chromatography with quadrupole mass spectrometry (GC×GC/qMS) was employed to assess the CHCs of L. geometricus females and juveniles of different ages. The results demonstrate that there is variation in CHCs of different body parts of females, with abdomen presenting greater number of compounds, as well as between adults and juveniles. Branched alkanes represented the majority of compounds in all samples, followed by linear alkanes, and alkenes were only present in adult’s abdomen and cephalothorax. The compounds 10-methyloctacosaneand 14-methyloctacosane; and 10-methyltriacontane and 14-methyltriacontane showed the same retention time in the 1D and were separated in the 2D. According to the results, rapid-scanning GC×GC/qMS can be considered a reliable technique to assess CHCs since it was possible to identify the spider’s cuticular compounds, and detect and separate two cases of chromatographic coelution.
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References
ABALOS JW. The egg-sac in the identification of species of Latrodectus (black-widow spiders). Psyche. 1962;69:268–270.
ANDRADE MCB, KASUMOVIC MM. Terminal investment strategies and male mate choice: extreme tests of Bateman. Integr. Comp. Biol. 2005;45:838-847.
ANTONIALLI-JUNIOR WF, LIMA SM, ANDRADE LHC, SÚAREZ YR. Comparative study of the cuticular hydrocarbon in queens, workers and males of Ectatomma vizottoi (Hymenoptera, Formicidae) by Fourier transform infrared photoacoustic spectroscopy. Genet. Mol. Res. 2007;6:492-499.
ANTONIALLI-JUNIOR WF, SÚAREZ YR, IZIDA T, ANDRADE LHC, LIMA SM. Intra- and interspecific variation of cuticular hydrocarbon composition in two Ectatomma species (Hymenoptera: Formicidae) based on Fourier transform infrared photoacoustic spectroscopy. Genet. Mol. Res. 2008;7:1-9.
BALA M, SHARMA A. Review of some recent techniques of age determination of blow flies having forensic implications. Egypt. Jour. Forensic Sci. 2016;6:203-208.
BARUFFALDI L, ANDRADE MCB. Contact pheromones mediate male preference in black widow spiders: avoidance of hungry sexual cannibals? Anim. Behav. 2015;102:25-32.
BARUFFALDI L, COSTA FG. Changes in male sexual responses from silk cues of females at different reproductive states in the wolf spider Schizocosa malitiosa. J. Ethol. 2010;28:75-85.
BARUFFALDI L, COSTA FG. Male reproductive decision is constrained by sex pheromones produced by females. Behaviour 2014;151:465-477.
BERTANI R, FUKUSHIMA CS, MARTINS R. Sociable widow spiders? Evidence of subsociality in Latrodectus Walckenaer, 1805 (Araneae, Theridiidae). J. Ethol. 2008;26:299-302.
BONAVITA-COUGOURDAN A, THERAULAZ G, BAGNÈRES AG, ROUX M, PRATTE M, PROVOST E, et al. Cuticular hydrocarbons, social organization and ovarian development in a polistine wasp: Polistes dominulus christ. Comp. Biochem. Physiol. B. 1991;100:667-680.
BROWN WV, SPRADBERY JP, LACEY MJ. Changes in the cuticular hydrocarbon composition during development of the social wasp, Vespula germanica (F.) (Hymenoptera: Vespidae). Comp. Biochem. Physiol. B. 1991;99:553-562.
COSTELLO MJ, DAANE KM. Influence of ground cover on spider populations in a table grape vineyard. Ecol. Entomol. 1998;23:33-40.
DANI FR, JONES GR, CORSI S, BEARD R, PRADELLA D, TURILLAZZI S. Nestmate recognition cues in the honey bee: differential importance of cuticular alkanes and alkenes. Chem. Senses. 2005;30:477-489.
DANI FR, JONES GR, DESTRI S, SPENCER SH, TURILLAZZI S. Deciphering the recognition signature within the cuticular chemical profile of paper wasps. Anim. Behav. 2001;62:165-171.
DEKEIRSSCHIETER J, STEFANUTO P-H, BRASSEUR C, HAUBRUGE E, FOCANT J-F. Enhanced characterization of the smell of death by comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS). PLoS ONE 2012;7 e39005.
DONDALE CD, HEGDEKAR BM. The contact sex pheromone of Pardosa lapidicina Emerton (Araneida: Lycosidae). Can. J. Zool. 1973;51:400-401.
EDMONDS DT, VOLLRATH F. The contribution of atmospheric water vapour to the formation and efficiency of spider’s capture web. Proc. R. Soc. B. 1992;248:145-148.
FOELIX RF. Biology of spiders. 1st ed. Oxford: Oxford University Press; 2011.
GARB JE, GONZÁLEZ A, GILLESPIE RG. The black widow spider genus Latrodectus (Araneae: theridiidae): phylogeny, biogeography, and invasion history. Mol. Phylogenet. Evol. 2004;31:1127-1142.
GASKETT AC. Spiders sex pheromones: emission, reception, structures, and functions. Biol. Rev. 2007;82:27-48.
GRINSTED L, BILDE T, D’ETTORRE P. Cuticular hydrocarbons as potential kin recognition cues in a subsocial spider. Behav. Ecol. 2011;22:1187-1194.
GRUNSHAW JP, GUERMOUCHE H, GUERMOUCHE S, JAGO ND, JULLIEN R, KNOWLES E, et al. Chemical taxonomic studies of cuticular hydrocarbons in locusts of the Schistocerca americana complex (Acrididae: Cyrtacanthacridinae): chemical relationships between new world and old world species. J. Chem. Ecol. 1990;16:2835-2858.
GUIMARÃES IC, CARDOSO CAL, LIMA SM, ANDRADE LHC, ANTONIALLI-JUNIOR WF. Chemical signals might mediate interactions between females and juveniles of Latrodectus geometricus (Araneae: Theridiidae). Behav. Process. 2016;126:27-35.
ISAACMAN G, WILSON KR, CHAN AWH, WORTON DR, KIMMEL JR, NAH T, et al. Improved resolution of hydrocarbon structures and constitutional isomers in complex mixtures using gas chromatography-vacuum ultraviolet-mass spectrometry. Anal. Chem. 2012;84:2335-2342.
JOHANSSON BG, JONES TM. The role of chemical communication in mate choice. Biol. Rev. 2007;82:265-289.
LEVI HW, LEVI LR, ZIM HS. Spiders and their kin. 1st ed.New York: S.t. Martin’s Press, New York; 2001.
LIU Z, PHILLIPS JB. Comprehensive two-dimensional gas chromatography using an on-column thermal modulator interface. J. Chromatogr. Sci. 1991;29:227-231.
LOTZ LN. Revision of the genus Latrodectus (Araneae: Theridiidae) in Africa. Navors. Nas. Mus. 1994;10:1-60.
MA T, XIAO Q, YU Y, WANG C, ZHU C, SUN Z, et al. Analysis of tea geometrid (Ectropis grisescens) pheromone gland extracts using GC-EAD and GC×GC/TOFMS. J. Agric. Food Chem. 2016;64:3161-3166.
MICHELUTTI KB, CARDOSO CAL, ANTONIALLI-JUNIOR WF. Evaluation of chemical signatures in the developmental stages of Mischocyttarus consimilis Zikán (Hymenoptera, Vespidae) employing gas chromatography coupled to mass spectrometry. Rev. Virtual Quim. 2017;9:535-547.
MONDELLO L, TRANCHIDA PQ, DUGO P, DUGO G. Comprehensive two-dimensional gas chromatography-mass spectrometry: a review. Mass. Spectrom. Rev. 2008;27:101-124.
MÜLLER GJ. Black and brown widow spider bites in South Africa. A series of 45 cases. S. Afr. Med. J. 1993;83:399-405.
NEVES EF, ANDRADE LHC, SÚAREZ YR, LIMA SM, ANTONIALLI-JUNIOR WF. Age-related changes in the surface pheromones of the wasp Mischocyttarus consimilis (Hymenoptera: Vespidae). Genet. Mol. Res. 2012;11:1891-1898.
NEVES EF, MONTAGNA TS, ANDRADE LHC, SÚAREZ YR, LIMA SM, ANTONIALLI-JUNIOR WF. Social parasitism and dynamics of cuticular hydrocarbons in paper wasps of the genus Mischocyttarus. J. Kanas. Entomol. Soc. 2013;86:69-77.
ÖZÇIMEN D, KARAOSMANOĞLU F. Production and characterization of bio-oil and biochar from rapeseed cake. Renew. Energ. 2004;29:779-787.
PASQUET A, TRABALON M, BAGNÈRES AG, LEBORGNE R. Does group closure exist in the social spider Anelosimus eximius? Behavioural and chemical approaches. Insect. Soc. 1997;44:159-169.
POURIÉ G, IBARRA F, FRANCKE W, TRABALON M. Fatty acids mediate aggressive behavior in the spider Tegenaria atrica. Chemoecology 2005;15:161-166.
POURIÉ G, TRABALON M. Agonistic behaviour of female Tegenaria atrica in the presence of different aged spiderlings. Physiol. Entomol. 1999;24:143-149.
POURIÉ G, TRABALON M. Plasticity of agonistic behaviour in relation to diet and contact signals in experimentally group-living of Tegenaria atrica. Chemoecology 2001;11:175-181.
PROUVOST O, TRABALON M, PAPKE M, SCHULZ S. Contact sex signals on web and cuticle of Tegenaria atrica (Araneae, Agelenidae). Arch. Insect. Biochem. Physiol. 1999;40:194-202.
RALSTON-HOOPER K, HOPF A, OH C, ZHANG X, ADAMEC J, SEPÚLVEDA MS. Development of GCxGC/TOF-MS metabolomics for use in ecotoxicological studies with invertebrates. Aquat. Toxicol. 2008;88:48-52.
STOLTZ JA, MCNEIL JN, ANDRADE MCB. Males assess chemical signals to discriminate just-mated females from virgins in red back spiders. Anim. Behav. 2007;74:1669-1674.
TORRES VO, SGUARIZI-ANTONIO D, LIMA SM, ANDRADE LHC, ANTONIALLI-JUNIOR WF. Reproductive status of the social wasp Polistes versicolor (Hymenoptera, Vespidae). Sociobiology 2014;61:218-224.
TRABALON M. Agonistic interactions, cuticular and hemolymphatic lipid variations during the foraging period in spider females Brachypelma albopilosa (Theraphosidae). J. Insect Physiol. 2011;57:735-743.
TRABALON M, ASSI-BESSÉKON D. Effects of web chemical signatures on intraspecific recognition in a subsocial spider, Coelotes terrestris (Araneae). Anim. Behav. 2008;76:1571-1578.
TRABALON M, BAGNÈRES AG, HARTMANN N, VALLET AM. Changes in cuticular compounds composition during the gregarious period and after dispersal of the young in Tegenaria atrica (Araneae, Agelenidae). Insect Biochem. Mol. Biol. 1996;26:77-84.
TRABALON M, BAGNÈRES AG, ROLAND C. Contact sex signals in two sympatric spider species, Tegenaria domestica and Tegenaria pagana. J. Chem. Ecol. 1997;23:747-758.
TRABALON M, NIOGRET J, LEGRAND-FROSSI C. Effect of 20-hydroxyecdysone on cannibalism, sexual behavior, and contact sex pheromone in the solitary female spider, Tegenaria atrica. Gen. Comp. Endocr. 2005;144:60-66.
TRABALON M, POURIÉ G, HARTMANN N. Relationship among cannibalism, contact signals, ovarian development and ecdysteroid levels in Tegenaria atrica (Araneae, Agelenidae). Insect Biochem. Mol. Biol. 1998;28:751-758.
UETZ GW, ROBERTS JA. Multisensory cues and multimodal communication in spiders: insights from video/audio playback studies. Brain Behav. Evol. 2002;59:222-230.
UHL G. Spider olfaction: attracting, detecting, luring and avoiding. In: NENTWIG W, editor. Spider Ecophysiology. Berlin: Springer-Verlag Berlin Heidelberg; 2013. p. 141-157.
VAN ZWEDEN JS, DREIER S, D’ETTORRE P. Disentangling environmental and heritable nestmate recognition cues in a carpenter ant. J. Insect Physiol. 2009;55:158-163.
WORK RW, MOROSOFF NA. A physico-chemical study of the super contraction of spider major ampullate silk fibers. Text. Res. J. 1982;52:349-356.
WORK RW, YOUNG CT. The amino acid compositions of major and minor ampullate silks of certain orb-web building spiders (Araneae, Araneidae). J. Arachnol. 1987;15:65-80.
WYATT TD. Pheromones and animal behavior: communication by smell and taste. 1st ed. Cambridge: Cambridge University Press; 2003.
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