Linear alkanes as a tool to evaluate intraspecific differences in social wasps

Kamylla Balbuena Michelutti, Claudia Andrea Lima Cardoso, William Fernando Antonialli-Junior


Cuticular hydrocarbons are found in the epicuticle of insects and act during interactions between nestmates in social insects. Among the classes of these compounds, branched alkanes stand out acting as flags during intracolonial interactions, therefore, varying significantly intraspecificaly. However, there is evidence that linear alkanes can also act as signals in these interactions and therefore would also be important to assess intraspecific differences in colonies of social insects. Thus, the hypothesis of this study is that linear alkanes can be used as a tool to establish intraspecific relationships in a species of social wasp. Linear alkanes of all developmental stages were evaluated from colonies of the species Mischocyttarus consimilis Zikán (Hymenoptera, Vespidae). 21 linear alkanes were detected, with the majority ranging from n-C26 to n-C33. There is significant variation between the compounds of all developmental stages, both from samples of different colonies and populations. Therefore, the results validate the hypothesis that variation in composition of linear alkanes can be useful to assess intraspecific differences in social wasps.


Developmental stages; Epicuticle; Nest material; Wasp population

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BLOMQUIST GJ, BAGNÉRES AG. Insect hydrocarbons biology, biochemistry, and chemical ecology. Published in the United States of America by Cambridge University Press, New York, 2010; p 492.

BONAVITA-COUGOURDAN A, THERAULAZ G, BAGNÉRES AG, ROUX M, PRATTE M, PROVOST E, CLÉMENT JL. Cuticular hydrocarbons, social organization and ovarian development in a polistine wasp: Polistes dominulus christ. Comp. Biochem. Physiol. 1991; 100B (4): 667–680.

BORGES AA, FERREIRA-CALIMAN MJ, NASCIMENTO FS, CAMPOS LAO, TAVARES MG. Characterization of cuticular hydrocarbons of diploid and haploid males, workers and queens of the stingless bee Melipona quadrifasciata. Insect. Soc. 2012.

BRITO JH dos S, MONTAGNA TS, MAIA FS, ANTONIALLI-JUNIOR WF, CARDOSO CAL. Cuticular signature in the development of Polistes versicolor. Genet. Mol. Res. 2015; 14 (4): 12520–12528.

BROWN WV, SPRADBERY JP, LACEY MJ. Changes in the cuticular hydrocarbon composition during development of the social wasp, Vespula germanica (f.) (Hymenoptera: Vespidae). Comp. Biochem. Physiol. 1991; 99(3): 553–562.

COTONESCHI C, DANI FR, CERVO R, SLEDGE MF, TURILLAZZI S. Polistes dominulus (Hymenoptera: Vespidae) larvae possess their own chemical signatures. J. Insect Physiol. 2007; 53: 954–963.

COTONESCHI C, DANI FR, CERVO R, SCALA C, STRASSMANN JE, QUELLER DC, TURILLAZZI S. Polistes dominulus (Hymenoptera, Vespidae) larvae show different cuticular patterns according to their sex: workers seem not use this chemical information. Chem. Senses. 2009; 53: 195–202.

DANI FR, MORGAN ED, TURILLAZZI S. Dufour gland secretion of Polistes wasp: chemical composition and possible involvement in nestmate recognition (Hymenoptera: Vespidae). J. Insect Physiol. 1996; 42(6): 541–548.

DANI FR, FOSTER KR, ZACCHI F, SEPPA P, MASSOLO A, CARELLI A, ARÉVALO E, QUELLER DC, STRASSMAN JE, TURILLAZZI S. Can epicuticular lipids provide sufficient information for within-colony nepotism in wasps? The Royal Society. Proc. R. Soc. Lond. B. 2004; 271: 745–753.

DAPPORTO L, THEODORA P, SPACCHINI C, PIERACCINI G, TURILLAZZI S. Rank and epicuticular hydrocarbons in different populations of the paper wasp Polistes dominulus (Christ) (Hymenoptera,Vespidae). Insect. Soc. 2004a; 51: 279–286.

DAPPORTO L, PALAGI E, TURILLAZZI S. Cuticular hydrocarbons of Polistes dominulus as a biogeographic tool: a study of populations from the tuscan archipelago and surrounding areas. J. Chem. Ecol. 2004b; 30(11): 2139–2151.

ESPELIE KE, HERMANN HR. Surface lipids of the social wasp Polistes annularis (L.) and its nest and nest pedicel. J. Chem. Ecol. 1990; 16(6): 1841–1852.

FERREIRA AC, CARDOSO CAL, NEVES EF, SÚAREZ YR, ANTONIALLI-JUNIOR WF. Distinct linear hydrocarbon profiles and chemical strategy of facultative parasitism among Mischocyttarus wasps. Genetics and Molecular Research. 2012; 11(4): 4351-435.

FERREIRA AC, NEVES EF, MONTAGNA TS, MENDONÇA A, CARDOSO CAL., ANTONIALLI-JUNIOR WF. Intraspecific Variation of the Composition of Linear Alkanes in Social Wasp Mischocyttarus consimilis. 2017; Sociobiology. 64(4): 466-476.

GULLAN PJ, CRANSTON OS. Os Insetos: Um resumo de entomologia. 3ª ed. Roca, São Paulo. 2007; p 440.

HEFETZ A. The evolution of hydrocarbon pheromone parsimony in ants (Hymenoptera: Formicidae) – interplay of colony odor uniformity and odor idiosyncrasy. A review. Myrmecological News. 2007; 10: 59–68.

HOWARD RW. Cuticular hydrocarbon and chemical communication. In: (Stanley-Samuelson DW, Nelson DR eds.) Insect lipids: chemistry, biochemistry and biology. University of Nebraska Press, Lincoln. 1993; pp 179–226.

HOWARD RW, BLOMQUIST GJ. Ecological, behavioral, and biochemical aspects of insect hydrocarbons. Annu. Rev. Entomol. 2005; 50: 371–393.

JEANNE, RL. Social biology of the neotropical wasp Mischocyttarus drewseni. Bulletin of the Museum of Comparative Zoology. 1972; 144: 63–150.

KLAHN JE, GAMBOA GJ. Social Wasps: Discrimination between kin and nonkin brood. Science. 1983; 221: 482–484.

LORENZI MC, BAGNÈRES AG, CLÉMENT JL, TURILLAZZI S. Polistes biglumis bimaculatus epicuticular hydrocarbons and nestmate recognition (Hymenoptera, Vespidae). Insectes Soc. 1997; 44: 123–138.

LORENZI MC, SLEDGE MF, LAIOLO P, STURLINI E, TURILLAZZI S. Cuticular hydrocarbon dynamics in young adult Polistes dominulus (Hymenoptera: Vespidae) and the role of linear hydrocarbons in nestmate recognition systems. J. Insect

Physiol. 2004; 50: 935–941.

MARTIN SJ, JONES GR, CHÂLINE N, RATNIEKS FLW. Role of hydrocarbons in egg recognition in the honeybee. Physiol. Entomol. 2004; 29: 395–399.

MICHELUTTI KB, CARDOSO CAL, ANTONIALLI-JUNIOR WF. Evaluation of chemical signatures in the developmental stages of Mischocyttarus consimilis Zikán (Hymenoptera, Vespidae) employing gas chromatography coupled to mass

spectrometry. Rev. Virtual Quim. 2017; 9(2): 535–547.

MURAKAMI ASN, NUNES TM, DESUÓ IC, SHIMA SN, MATEUS S. The cuticular hydrocarbons profiles in the colonial recognition of the Neotropical eusocial wasp, Mischocyttarus cassununga (Hymenoptera: Vespidae). Sociobiology. 2015;

(1): 109–115.

O’DONNELL S. Reproductive caste determination in eusocial wasps (Hymenoptera: Vespidae). Annu. Rev. Entomol.1998; 43: 323–346.

PANEK LM, GAMBOA GJ. Queens of the paper wasp Polistes fuscatus (Hymenoptera: Vespidae) discriminate among larvae on the basis of relatedness. Ethology. 2000; 106: 159–170.

PARRA JRP, HADDAD ML. Determinação do número de instares de insetos. Piracicaba: FEALQ. 1989; p 49.

ROSSI AM, HUNT JH. Honey supplementation and its developmental consequences: evidence for food limitation in a paper wasp, Polistes metricus. Ecol. Entomol. 1988; 13: 437–442.

SILVA ERS, MICHELUTTI KB, ANTONIALLI-JUNIOR WF, BATISTOTE M, CARDOSO CAL. Chemical signatures in the developmental stages of Protopolybia exigua. Genet. Mol. Res. 2016; 15(1):1–12.

SINGER TL, ESPELIE KE. Social wasps use nest paper hydrocarbons for nestmate recognition. Anim. Behav. 1992; 44: 63–68.

TANNURE-NASCIMENTO IC, NASCIMENTO FS, TURATTI IC, LOPES NP, TRIGO JR, ZUCCHI R. Colony membership is reflected by variations in cuticular hydrocarbon profile in a Neotropical paper wasp, Polistes satan (Hymenoptera,

Vespidae). Genet. Mol. Res. 2007; 6 (2): 390–396.

TANNURE-NASCIMENTO IC, NASCIMENTO FS, ZUCCHI R. The look of royalty: visual and odour signals of reproductive status in a paper wasp. Proc. R. Soc. B. 2008; 275: 2555–2561.

WENZEL JW. A generic key to the nests of hornets, yellowjackets, and paper wasps worldwide (Vespidae: Vespinae, Polistinae). Am. Mus. Novit. 1998; 3224: 1–39.

ZHU GH, YE GY, HU C, XU XH, LI K. Development changes of cuticular hydrocarbons in Chrysomya rufifacies larvae: potential for determining larval age. Med. Vet. Entomol. 2006; 2: 438-444.


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