Molecular characterization and vegetative compatibility groups of <i>Fusarium</i> spp. pathogenic to <i>Ilex paraguariensis</i> A. St.-Hil.
DOI:
https://doi.org/10.5902/1980509846843Parole chiave:
ITS, β-tubulin, nitAbstract
In the central-western and southern regions of Brazil, the cultivation of yerba mate (Ilex paraguariensis) is considered an important source of forest extraction, besides providing an alternative income, especially for families. The yerba mate, like other forest species, is infected by several pathogens, such as fungi of the genus Fusarium, causing root rot. Pathogens have wide genetic variability, even within a single genus and species, resulting from adaptations to their environments. This study aimed at characterizing molecularly isolated Fusarium spp. through the sequencing of the ITS and β-tubulin genome regions, identifying the species level as well as the formation of vegetative compatibility groups (VCG) among these isolates of the root rot pathogen in yerba mate. The sequencing of the ITS and β-tubulin regions, defined the separation between the seven pathogenic isolates of F. solani and F. oxysporum. For the same isolates, the technique to obtain nit mutants was suitable for the phenotypic classification, obtaining five VCGs for species of Fusarium, root rot in yerba mate.
Downloads
Riferimenti bibliografici
ALCÁZAR, M. G. et al. Grupos de compatibilidad vegetativa de Fusarium oxysporum f. sp. radicis-cucumerinum em la Provincia de Alméria. Boletín de Sanidad Vegetal Plagas, Madrid, v. 32, n. 1, p. 535-543, 2006.
ALTINOK, H. H.; CAN, C.; ÇOLAK, H. Vegetative Compatibility, Pathogenicity and Virulence Diversity of Fusarium oxysporum f. sp. melongenae Recovered from Eggplant. Journal Phytopathology, Berlin, v. 161, n. 9, p. 651-660, 2013.
ALVARADO, G. H.; RESENDES, I. B.; FERNÁNDEZ, R. R. Caracterización de Grupos de Compatibilidad Vegetativa de Fusarium mexicanum Causante de la Malformación del Mango en Jalisco, México. Revista Mexicana de Fitopatología, Texcoco, v. 30, n. 2, p. 128-140, 2012.
BOGALE, M. et al. Diverse Fusarium solani isolates colonise agricultural environments in Ethiopia. European Journal of Plant Pathology, Dordrecht, v. 124, n. 3, p. 369-378, 2009.
CORRELL, J. C.; PUHALLA, J. E.; SCHNEIDER, R. W. Identification of Fusarium oxysporum f. sp. apiion the basis of colony size, virulence, and vegetative compatibility. Phytopathology, St. Paul, v. 76, n. 4, p. 396-400, 1986.
CRESPO, N. C. Diversidade genética de isolados do agente etiológico da fusariose do abacaxizeiro no Brasil. 2010. (Mestrado em Agronomia) - Universidade Federal de Lavras, Lavras, 2010.
DATTA, S. et al. Polymorphism in the internal transcribed spacer (ITS) region of the ribosomal DNA among different Fusarium species. Archives of Phytopathology and Plant Protection, [s. l.], v. 44, n. 6, p. 558-566, 2011.
DIAS, J. S. A.; ABREU, M. S.; RESENDE, M. L. V. Caracterização de isolados de Fusarium oxysporum f. sp. cubense (Foc) quanto à compatibilidade vegetativa e à patogenicidade em cultivares de bananeira diferenciadoras de raças no Brasil. Biota Amazônia, Macapá, v. 4, n. 4, p. 60-65, 2014.
DOYLE, J. J.; DOYLE, J. L. Isolation of plant DNA from fresh tissue. Focus, [s. l.], v. 12, n. 1, p. 13-15, 1991.
ELENA, K.; PAPPAS, A. C. Pathogenicity and Vegetative Compatibility of Fusarium oxysporum f. sp. phaseoli in Greece. Journal Phytopathology, Berlin, v. 150, n. 8-9, p. 495-499, 2002.
FUNDO DE DESENVOLVIMENTO E INOVAÇÃO DA CADEIA PRODUTIVA DE ERVA-MATE. Guia da erva-mate. Porto Alegre, 2014. 15 p.
GLASS, N. L.; JACOBSON, D. J.; SHIU, P. K. The genetics of hyphal fusion and vegetative incompatibility in filamentous ascomycetes. Annual Review of Genetics, Palo Alto, v. 34, p. 165-186, 2000.
GRIGOLETTI JUNIOR, A.; AUER, C. G.; MASCHIO, L. M. A. Doenças em Erva mate (Ilex paraguariensisA. St.-Hill) na região sul do Brasil. Boletim de Pesquisa Florestal, Colombo, v. 32/33, p. 43-51, 1996.
HALL, T. A. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium, Oxford, v. 41, p. 95-98, 1999.
JACOBS, A. et al. Fusarium ananatum sp. nov. in the Gibberella fujikuroi species complex from pineapples with fruit rot in South Africa. Fungal Biology, Oxford, v. 114, n. 7, p. 515-527, 2010.
ILIĆ, J. et al. Vegetative compatibility of Fusarium oxysporum isolated from weeds in eastern Croatia. Poljoprivreda, Osijek, v. 19, n. 1, p. 20-24, 2013.
JOST, W. et al. A large plant β-tubulin family with minimal C-terminal variation but differences in expression. Gene, [s. l.], v. 340, n. 1, p. 151-160, 2004.
JUSOH, M. N. B.; ZIN, N. B. M.; NAGAO, H. Vegetative compatibility group of Fusarium solani pathogenic to tobacco plant in peninsular Malaysia. Songklanakarin Journal of Science and Technology, Hat Yai, v. 35, n. 6, p. 615-621, 2013.
KIPROP, E. K. et al. Cultural characteristics, pathogenicity and vegetative compatibility of Fusarium udum isolates from pigeonpea (Cajanuscajan (L.) Millsp.) in Kenya. European Journal of Plant Pathology, Dordrecht, v. 108, n. 2, p. 147-154, 2002.
LESLIE, J. F. Fungal vegetative compatibility. Annual Review of Phytopathology, Palo Alto, v. 31, p. 127-150, 1993.
LESLIE, J. F.; SUMMERELL, B. A. The fusarium laboratory manual. Ames: Blackwell Publishing, 2006. 388 p.
MEZZOMO, R. et al. Morphological and molecular characterization of Fusarium spp. pathogenic to Ilex Paraguariensis. Cerne, Lavras, v. 24, n. 3, p. 209-218, 2018.
PUHALLA, J. E. Classification of strains of Fusarium oxysporum on the bases of vegetative compatibility. Canadian Journal of Botany, Ottawa, v. 63, n. 2, v. 179-183, 1985.
ROSALEE, A.; COELHO, N.; DHINGRA, O. D. Grupos de compatibilidade vegetativa entre insolados de Fusarium oxysporum não patogênicos ao feijoeiro e de F. oxysporum f. sp. phaseoli. Fitopatologia Brasileira, Brasília, v. 24, n. 4, p. 546-548, 1999.
SAABALE, P. R.; DUBEY, S. C. Pathogenicity and vegetative compatibility grouping among Indian populations of Fusarium oxysporum f. sp. ciceris causing chickpea wilt. Phytoparasitica, Bet Dagan, v. 42, n. 11-12, p. 465-473, 2014.
SHAHNAZI, S. et al. Morphological and molecular characterization of Fusarium spp. associated with yellowing disease of black pepper (Piper nigrum L.) in Malaysia. Journal of General Plant Pathology, [s. l.], v. 78, n. 3, p. 160-169, 2012.
TAMURA, K. et al. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, Oxford, v. 24, n. 8, p. 1596-1599, 2007.
TEIXEIRA, H.; VIEIRA, M. G. G. C.; MACHADO, J. C. Marcadores morfofisiológicos e isoenzimáticos na análise da diversidade genética de isolados de Acremonium strictum. Fitopatologia Brasileira, Brasília, v. 29, n. 4, p. 413-418, 2004.
VISENTIN, I. et al. The ITS region as a taxonomic discriminator between Fusarium verticillioides and Fusarium proliferatum. Mycological Research, London, v. 113, n. 10, p. 1137-1145, 2009.
WHITE, T. J. et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: INNIS, M. A. et al. Comparative evolutionary analysis of rDNA ITS regions in Drosophila. Molecular Biology and Evolution, Oxford, v. 11, n. 3, p. 513- 522, 1990.
##submission.downloads##
Pubblicato
Come citare
Fascicolo
Sezione
Licenza
A CIÊNCIA FLORESTAL se reserva o direito de efetuar, nos originais, alterações de ordem normativa, ortográfica e gramatical, com vistas a manter o padrão culto da lingua, respeitando, porém, o estilo dos autores.
As provas finais poderão ou não ser enviadas ao autor.
Os trabalhos publicados passam a ser propriedade da revista CIÊNCIA FLORESTAL, sendo permitida a reprodução parcial ou total dos trabalhos, desde que a fonte seja citada. Os originais não serão devolvidos aos autores.
As opiniões emitidas pelos autores dos trabalhos são de sua exclusiva responsabilidade.
