<i>Trichoderma asperellum</i> in the control of stem canker in <i>Carya illinoinensis</i>
DOI:
https://doi.org/10.5902/1980509872270Keywords:
Trunk canker, Lasiodiplodia theobromae, Biological control, Disease managementAbstract
The objective of this work was to evaluate the ability of Trichoderma asperellum isolates and their culture filtrates to control Lasiodiplodia theobromae in vivo and to promote the growth of pecan seedlings. Two experiments were carried out, both in a greenhouse. In the first experiment, the aerial part of the plants was sprayed by biological control treatments and, 14 days later, the pathogen was inoculated into the stem, through injury. In the second experiment, the substrate was moistened with biological control treatments and 14 days later the pathogen was inoculated following the same methodology as the first experiment. The following parameters were evaluated: length of the aerial part, stem diameter, fresh mass of the aerial part and the root, dry mass of the aerial part and the root, incidence and severity of the disease. The comparison of means was performed using the Skott-Knott test at a 5% probability of error, using the SISVAR software. In both experiments, the greatest responses were obtained in the growth parameters evaluated in the treatments with the culture filtrates and with the spore suspension in relation to the treatment inoculated with the pathogen without the use of biological control treatments. These treatments also reduced the incidence and severity of stem canker in pecan seedlings, and the best treatment varied with the form of application. The isolates of T. asperellum and the culture filtrates stimulated the initial growth of the plants, decreasing the incidence and severity of stem canker in pecan plants, regardless of the form of application.
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ABDOLLAHZADEH, J.; JAVADI, A.; GOLTAPEH, E. M.; ZARE, R.; PHILLIPS, A. J. L. Phylogeny and morphology of four new species of Lasiodiplodia from Iran. Persoonia. v. 25, p. 1–10, 2010.
AGUIAR, A. R. de; AGUIAR, D.; TEDESCO, S. B.; SILVA, A. C. F. da. Efeito de metabólitos produzidos por Trichoderma spp. sobre o índice mitótico em células das pontas de raízes de Allium cepa. Bioscience Journal. v. 31, n. 3, p. 934-940, 2015.
AMARAL, P. P.; STEFFEN, G. P. K.; MALDANER, J.; MISSIO, E. L.; SALDANHA, C. W. Promotores de crescimento na propagação de caroba. Pesquisa florestal brasileira. v. 37, n. 90, p. 149-157, 2017.
BORGES, R. C. F.; MARQUES, E.; MACEDO, M. A.; MARTINS, I.; SILVA FILHO, J. G. da; MELLO, S. C. M. de. Biocontrol of teak canker caused by Lasiodiplodia theobromae. Revista Árvore. v. 42, n. 3, 2018.
BRITO, R. A. S.; CAVALCANTE, G. P.; STOCK, V. M.; COLMAN, A. A.; SANTOS, D. P. DOS; SERMARINI, R. A.; MAFFIA, L. A. Trichoderma species show biocontrol potential against Ceratocystis wilt in mango plants. European Journal of Plant Pathology, v. 158, n. 3, p. 781-788, 2020.
FERREIRA, D. F. Sisvar: um sistema computacional de análise estatística. Ciência e Agrotecnologia, v. 35, n. 6, p. 1039–1042, 2011.
JUNGES, E.; MUNIZ, M. F. B.; CAMPOS, A. D.; BRUN, T.; MICHELON, C. J.; MAZUTTI, M. A. Bioproducts from Trichoderma harzianum as inducer of resistance to anthracnose in beans. In: SANTOS, A. S. (org.). Avanços Científicos e Tecnológicos em Bioprocessos. Atena, 2018. P.70-80.
LUO, Y.; LICHTEMBERG, P. S. F.; NIEDERHOLZER, F. J. A.; LIGHTLE, D. M.; FELTS, D. G.; MICHAILIDES, T. J. Understanding the Process of Latent Infection of Canker-Causing Pathogens in Stone Fruit and Nut Crops in California. Plant Disease, v. 103, n. 9, p. 2374–2384, 2019.
MAHDIZADEHNARAGHI, R.; HEYDARI, A.; ZAMANIZADEH, H. R.; REZAEE, S.; NIKAN, J. Biological control of garlic (Allium) whiterot disease using antagonistic fungi-based bioformulations. Journal of Plant Protection Research, v. 55, n. 2, p. 136–141, 2015.
MEYER, M. C.; MAZARO, S. M.; SILVA, J. C. da. Trichoderma: uso na agricultura. Brasília, DF: Embrapa, 538 p. 1ª ed. 2019.
MOUTASSEM, D.; BELABID, L.; BELLIK, Y. Efficiency of secondary metabolites produced by Trichoderma spp. in the biological control of Fusarium wilt in chickpea. Journal of Crop Protection, v. 9, n. 2, p. 217-231, 2020.
O'BRIEN P. A. Biological control of plant diseases. Australasian Plant Pathology, v. 46, p. 293-304, 2017.
POLETTO, T.; MACIEL, C. G.; MUNIZ, M.; BLUME, E.; POLETTO, I.; BRIOSO, P. First report of stem canker caused by Lasiodiplodia subglobosa on Carya illinoinensis in Brazil. Plant Disease, v. 100, p. 1016-1016, 2016.
RABUSKE, J. E. Trichoderma asperellum no manejo de doenças e na promoção de crescimento de plantas de nogueira-pecã. 2021. 174 f. Tese (Doutorado em Engenharia Florestal). Universidade Federal de Santa Maria, 2021.
ROLIM, J. M.; SAVIAN, L. G.; WALKER, C.; RABUSKE, J. E.; SARZI, J. S.; MUNIZ, M. F. B.; SILVA, J. C. P. da. First report of stem canker caused by Neofusicoccum parvum and Pseudofusicoccum kimberleyense on Carya illinoinensis in Brazil. Plant Disease, v. 16, 2020.
ROLIM, J. M.; RABUSKE, J. E.; SAVIAN, L. G.; WALKER, C.; SARZI, J. S.; SILVA, J. C. P. da; MUNIZ M. F. B. Fungi of the botryosphaeriaceae family cause different levels
of stem canker on pecan trees (Carya illinoinensis) in Brazil. Revista Arvore, 46, e4615, 2022.
SANTOS, R. F. dos; HECKLER, L. I.; LAZAROTTO, M.; GARRIDO, L. da R.; REGO, C.; BLUME, E. Trichoderma spp. and Bacillus subtilis for control of Dactylonectria macrodidyma in grapevine. Phytopathologia Mediterranea, v. 55, n. 2, p. 293−300, 2016.
SOLDAN, A.; WATZLAWICK, L. F.; BOTELHO, R. V.; FARIA, C. M. D. R.; MAIA, A. J. Development of forestry species inoculated with Trichoderma spp. Fertilized with rock phosphate. Floresta e Ambiente. v. 25, n. 4, p. 1-8, 2018.
TREMACOLDI, C. R.; LUNZ, A. M.; COELHO, I. L.; BOARI, A. Cancro em mogno africano no estado do Pará. Pesquisa Florestal Brasileira, v. 33, n. 74, p. 221-225, 2013.
WOO, S. L.; RUOCCO, M.; VINALE, F.; NIGRO, M.; MARRA, R.; LOMBARDI, N.; PASCALE, A.; LANZUISE, S.; MANGANIELLO, G.; LORITO, M. Trichoderma‐based products and their widespread use in agriculture. The Open Mycology Journal, v. 8, p. 71– 126, 2014.
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