LIGNIFICATION AND GROWTH OF<i> Eucalyptus grandis</i> (HILL EX MAIDEN) WITH GA3 AND BAP
DOI:
https://doi.org/10.5902/1980509822763Keywords:
Eucalyptus grandis, cell wall, lignin, forest biotechnology.Abstract
In this research experiments were accomplished with two classes of vegetable hormones (gibberellin and cytokinin); with the objective of verifying the effect of gibberellic acid and 6-Benzylaminopurine (GA3 and BAP) on the lignification and the longitudinal growth in seedlings of Eucalyptus grandis. The experiment was accomplished greenhouse, where were the growth regulators combined or isolated applied, in the following concentrations: GA3 (49.13 μM e 98.26 μM); BAP (111 μM e 222 μM), with five repetitions for each treatment. The comparisons among the averages of the height of the plants to the 6 months showed that the most pronounced prolongations of the stem corresponded to the treatments G[2], G[2]C[1] and G[2]C[2] where the largest concentration of GA3 was used. In the treatment G[1]C[1] where it was combined to smallest concentration of GA3 with to smallest concentration of BAP (49,13 μM de GA3 + 111 μM de BAP), it happened the smallest lignin tenor 23,37%.
Downloads
References
ABRANTES, F. L. et al. Uso de regulador de crescimento em cultivares de feijão de inverno. Pesquisa Agropecuária Tropical, Goiânia, v. 41, n. 2, p. 148-154, 2011.
BHATTACHARYA, A. et al. Practical Applications of Manipulating Plant Architecture by Regulating Gibberellin Metabolism. Journal of Plant Growth Regulation, v. 29, n. 2, p. 249-256, 2010.
BROWNING, B. L. Methods of wood chemistry. Interscience Publishers, New York, v. 1, p. 75-89, 1967.
CHEN, F., DIXON, R. A. Lignin Modification Improves Fermentable Sugar Yields for Biofuel Production. Nature Biotechnology, v. 25, p. 759-761, 2007.
DETTMER, J. et al. Hormone interactions during vascular development. Plant Molecular Biology, v. 69, p. 347–360, 2009.
EFFLAND, M. J. Modified procedure to determine acid-insoluble lignin in wood and pulp. Teppi, v. 60, n. 10, p. 143-144, 1977.
GALLEGO-GIRALDO, L. et al. Salicylic acid mediates the reduced growth of lignin down-regulated plants. Proceedings of the National Academy of Sciences of the United States of America, v. 108, n. 51, p. 20814-20819, 2011.
KAPCHINA-TOTEVA, V.; YAKIMOVA, E. Effect of purine and phenylureacytokinins on peroxidase activity in relation to apical dominance of in vitro cultivated Rosa hybrid L. Bulgarian Journal of Plant Physiology, v. 23, n. 1-2; p. 40-48, 1997.
LANA, A. M. Q. et al. Aplicação de reguladores de crescimento na cultura do feijoeiro. Bioscience Journal, v. 25, n. 1, p. 13-20, 2009.
LARA, M. E. B. et al. Extracellular invertase is an essential component of cytokinin-mediated delay of senescence. The Plant Cell, v. 16, p. 1276-1287, 2004.
LIN, S. Y.; DANCE, C. W. Methods in lignin chemistry. Berlim: Springer, 1992. 608 p.
MONTEIRO, M. B. O. et al. Análise composicional por espectrometria de infravermelho da lignina de Eucalyptus urophylla S. T. Blake tratados com reguladores de crescimento. Biochemistry and Biotechnology Reports, v. 1, n. 2, 2012.
MORK, D. W.; MORK, M. C. Cytokinin metabolism and action. Annual Review of Plant Physiology and Plant Molecular Biology, v. 52, p. 89–118, 2001.
MUJIB, A. et al. Catharanthusroseus alkaloids: application of biotechnology for improving yield. Plant Growth Regulation, v. 68, p. 111-127, 2012.
NISLER, J. et al. Cytokinin receptor antagonists derived from 6-benzylaminopurine. Phytochemistry, v. 71, p. 823–830, 2010.
PEREIRA, R. P. W. et al. Bioinformação do Processo de Lignificação. 1. ed. Seropédica: EDUR, 2012.
PHARIS, R. et al. Superior growth potential in trees: what is its basis, and can it be tested for at an early age. Canadian Journal of Forest Research, v. 21, p. 368-374, 1991.
QIN, F. et al. Spindly, a Negative Regulator of Gibberellic Acid Signaling, Is Involved in the Plant Abiotic Stress Response. Plant Physiology, v. 157, p. 1900–1913, 2011.
QUEIROZ, D. L. et al. Feeding and oviposition preferences of Ctenarytainaspatulata Taylor (Hemiptera, Psyllidae) for Eucalyptus spp. and other Myrtaceae in Brazil. Revista Brasileira de Entomologia, v. 54, n. 1, p. 149–153, 2010.
SHRIVASTAVA, B.; NANDALA, P. Solid state bioconversion of wheat straw into digestible and nutritive ruminant feed by Ganoderma sp. rckk02. Bioresource Technology, v. 107, p. 347–351, 2012.
SMOAK, E. M. et al. Self-assembly of gibberellic amide assemblies and their applications in the growth and fabrication of ordered gold nanoparticles. Nanotechnology, v. 21, n. 2, p. 25101-25704, 2010.
TOKUNAGA, N. et al. Involvement of gibberellin in tracheary element differentiation and lignification in Zinnia elegans xylogenic culture. Protoplasma, v. 228, n. 4, p. 179-187, 2006.
VALERI, S. V. Efeitos da adubação NPK e do calcário dolomítico no desenvolvimento de Eucalyptus grandis Hill ex Maiden. Silvicultura, São Paulo, v. 8, n. 28, p. 531-5366, 1983.
WERBROUCK, S. P. O. et al. Meta-topolin, an alternative to benzyladenine in tissue culture? Physiologia Plantarum, v. 98, p. 291–297, 1996.
ZHAO, Q. et al. Syringyl lignin biosynthesis is directly regulated by a secondary cell wall master switch. Proceedings of the National Academy of Sciences, v. 107, p. 14496–14501, 2010.
ZIEBELL, A. et al. Increase in 4-coumaryl alcohol units during lignification in alfalfa (Medicago sativa) alters the extractability and molecular weight of lignin. Journal of Biological Chemistry, v. 285, n. 50, p. 38961–38968, 2010.
