Ageratum conyzoides, uma espécie invasiva com potencial antioxidante e antifúngica

Autores

DOI:

https://doi.org/10.5902/2179460X83759

Palavras-chave:

Precocenos, β-cariofileno, Aspergillus ochraceus, Mentrasto

Resumo

Este estudo teve como objetivo avaliar a composição química e as atividades antioxidante e antifúngica do óleo essencial (OE) extraído das partes aéreas (folhas e flores) de Ageratum conyzoides (Asteraceae). O OE foi extraído pelo processo de hidrodestilação (3h), e os compostos identificados por cromatografia gasosa acoplada à espectrometria de massas (CG-EM). A atividade antioxidante foi avaliada pelo sistema de co-oxidação β-caroteno/ácido linoléico e pelo seqüestro de radicais livres 2,2-difenil-1-picril-hidrazil (DPPH) e pelo método de redução de ferro (FRAP). A atividade antifúngica foi realizada pelo método de microdiluição em caldo (MIC), utilizando as cepas Rhizopus oryzae ATCC 7560; Aspergillus flavus ATCC 1217; Aspergillus ochraceus ATCC 6787 e Penicillium verrucosum ATCC 7680. Os resultados indicaram um rendimento de 0,82 mg/kg. Hidrocarbonetos sesquiterpenos (33,28%) foram a classe majoritária e precoceno I (48,19%), precoceno II (7,38%) e β-cariofileno (19,66%) os constituintes majoritários. O sistema de co-oxidação β-caroteno/ácido linoléico apresentou 52,18% de inibição da oxidação na concentração de 1,0 mg/mL. Dos quatro fungos avaliados, apenas Aspergillus ochraceus apresentou resultado, com CIM de 1250 μL/mL, e a importância de encontrar atividade sobre esse fungo está no fato de ser produtor de ocratoxina A, infestando principalmente grãos de café verde. Os resultados encontrados abrem novas perspectivas na valorização de uma espécie considerada invasora.

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Biografia do Autor

Vanusa Souza Rocha Pereira, Universidade Paranaense

Mestrado em Biotecnologia Aplicada à Agricultura pela Universidade Paranaense.

Gabriela Catuzo Canônico Silva, Universidade Paranaense

Mestrado em Biotecnologia Aplicada à Agricultura pela Universidade Paranaense.

Edneide Bezerra da Cruz Oliveira, Universidade Paranaense

Graduação em Farmácia pela Universidade Paranaense.

Mariane de Almeida Machado, Universidade Paranaense

Graduação em Farmácia pela Universidade Paranaense.

Jéssica da Silva Sena, Universidade Paranaense

Mestrado em Biotecnologia Aplicada à Agricultura pela Universidade Paranaense.

Marisa Cássia Vieira de Araújo Bento, Universidade Paranaense

Mestrado em Ciência Animal pela Universidade Paranaense.

Selma Alves Rodrigues, Universidade Paranaense

Graduação em Medicina Veterinária pela Universidade Paranaense.

Rodrigo Sadao Inumaro, Centro Universitário Cesumar

Mestrado em Tecnologias Limpas pelo Centro de Ensino Superior de Maringá.

José Eduardo Gonçalves, Centro Universitário Cesumar

Professor pesquisador na área de química de produtos naturais da Universidade UniCesumar.

Maria Graciela Iecher Faria Nunes, Universidade Paranaense

Doutorado em Biotecnologia Aplicada à Agricultura pela Universidade Paranaense.

Suelen Pereira Ruiz Herrig, Universidade Paranaense

Doutorado em Ciência de Alimentos pela Universidade Estadual de Maringá.

Ranulfo Piau Junior, Universidade Paranaense UNIPAR

Doutorado em Biomedicina pelo Universidad de Léon, Espanha.

Zilda Cristiani Gazim, Universidade Paranaense

Doutorado em Ciências Farmacêuticas pela Universidade Estadual de Maringá.

Referências

Adams, R. P. (2017). Identification of essential oil components by gas chromatography /massspectrometry. Carol Stream, Illinois (USA): Allured. Recovered from: http://www.juniperus.org/uploads/2/2/6/3/22639912/bk4frontisbnpreface-contents5thedonline2017.pdf

Barbosa, K. B. F., Costa, N. M. B., Alfenas, R. C. G., De Paula, S. O., Minim, V. P. R., & Bressan, J. (2010). Estresse oxidativo: conceito, implicações e fatores modulatórios. Revista de Nutrição, 23, 629-643. Recovered from: https://doi.org/10.1590/S1415-52732010000400013

Barros, F. M. C., Almeida, P. C., Scopel, R., do Espirito Santo, A. T., Lucas, A. M., Bordignon, S. A. L., Cassel, E., Vargas, R. M. F., & Von Poser, G. (2015). Chromenes from Ageratum conyzoides: Steam distillation, supercritical extraction, and mathematical modeling. Separation Science and Technology, 51(2), 307-3015. Recovered from: https://doi.org/10.1080/01496395.2015.1086798

Basha, R. H. & Sankaranarayanan, C. (2016). β-Caryophyllene, a natural sesquiterpene lactone attenuates hyperglycemia mediated oxidative and inflammatory stress in experimental diabetic rats. Chemico-Biological Interactions, 245, 50–58. Recovered from: https://doi.org/10.1016/j.cbi.2015.12.019

Beloti, V., Barros, M. A. F., de Freitas, J. C., Nero, L. A., de Souza, J. A., Santana, E. H. W., & Franco, B. D. G. M. (1999). Frequency of 2,3,5-triphenyltetrazolium chloride (TTC) non-reducing bacteria in pasteurized milk. Revista de Microbiologia, 30, 137-140. Recovered from: https://doi.org/10.1590/S0001-37141999000200009

Benzie, I. F., & Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry, 239(1), 70-76. Recovered from: https://doi.org/10.1006/abio.1996.0292

Binder, B. E., Bowers, W. S., & Evans, P. H. (1991). “Insect anti-juvenile hormone and juvenile hormone activity from plants in the genus Mana” Experientia, 47(1), 199-201. https://doi.org/10.1007/BF01945427

Bettiol, W., & Morandi, M. A. B. (2009). Biocontrole de doenças de plantas: uso e perspectivas. Jaguariúna, São Paulo: Embrapa Meio Ambiente. Recovered from: http://www.alice.cnptia.embrapa.br/alice/handle/doc/579954

Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology, 94(3), 223-253. Recovered from: https://doi.org/10.1016/j.ijfoodmicro.2004.03.022

Castro, H. G. D., Oliveira, L. O. D., Barbosa, L. C. D. A., Ferreira, F. A., Silva, D. J. H. D., Mosquim, P. R., & Nascimento, E. A. (2004). Teor e composição do óleo essencial de cinco acessos de mentrasto. Química Nova, 27, 55-57. Recovered from: https://doi.org/10.1590/S0100-40422004000100011

Calleja, M. A., Vieites, J. M., Montero-Meterdez, T., Torres, M. I., Faus, M. J., Gil, A., & Suárez, A. (2013). The antioxidant effect of β-caryophyllene protects rat liver from carbon tetrachloride-induced fibrosis by inhibiting hepatic stellate cell activation. British Journal of Nutrition, 109(3), 394-401. Recovered from: https://doi.org/10.1017/S0007114512001298

Chabi-Sika, K., Sina, H., Boya, B., Salami, H. A., A Dossou, G., Mama-Sirou, I., Dansou, G., Socohou, A., Nounagnon, M., Lehmane, H., Adjanohoun, A., & Baba-Moussa, L. (2023). Ethnobotanical survey and some biological activities of Ageratum conyzoides collected in Southern-Benin. International Journal of Biochemistry Research & Review, 32(1), 9-25. Recovered from: https://doi.org/10.9734/ijbcrr/2023/v32i1793

De Castro, W. C., Almeida, R. V., Leite, M. B., Marrs, R. H., & Matos, D. S. (2016). Invasion strategies of the white ginger lily Hedychium coronarium J. König (Zingiberaceae) under different competitive and environmental conditions. Environmental and Experimental Botany, 127, 55-62. Recovered from: https://doi.org/10.1016/j.envexpbot.2016.03.010

Desai, D. H., Desai, H. S., & Chauhan, B. S. (2024). Germination attributes of metsulfuron-resistant and metsulfuron-susceptible tropical ageratum (Ageratum conyzoides) populations under various environmental conditions. Weed Science, 1-8. Recovered from: https://doi.org/10.1017/wsc.2024.31

Dhami, K. (2018). The terrestrial alien invasives of family Asteraceae in Punjab (India) and their ecology. International Journal of Advance Research, Ideas and Innovations in Technology, 4, 282-285. Recovered from: https://www.ijariit.com/manuscripts/v4i1/V4I1-1243.pdf

Dobhal, P., Purohit, V. K., Chandra, S., Rawat, S., Prasad, P., Bhandari, U., Trivedi, V. L., & Nautiyal, M. C. (2024). Climate-induced changes in essential oil production and terpene composition in Alpine aromatic plants. Plant Stress, 12, 100445. Recovered from: https://doi.org/10.1016/j.stress.2024.100445

Dores, R. G., Guimarães, S. F., Braga, T. V., Fonseca, M. C., Martins, P. M., & Ferreira, T. C. (2014). Phenolic compounds, flavonoids and antioxidant activity of leaves, flowers and roots of goat weed. Horticultura Brasileira, 32(4), 486-490. Recovered from: https://doi.org/10.1590/S0102-053620140000400019

Dũng, N. E. N. X., Tho, P. T. T., Dan, N. V., & Leclercq, P. A. (1989). Chemical composition of the oil of Ageratum conyzoides L. from Vietnam. Journal of Essential Oil Research, 1(3), 135-136. Recovered from: https://doi.org/10.1080/10412905.1989.9697769

Duarte-Almeida, J. M., Santos, R. J. D., Genovese, M. I., & Lajolo, F. M. (2006). Avaliação da atividade antioxidante utilizando sistema beta-caroteno/ácido linoléico e método de seqüestro de radicais DPPH•. Food Science and Technology, 26, 446-452. Recovered from: https://doi.org/10.1590/S0101-20612006000200031

Edwin, I. E., & Kester, U. E. (2018). Insecticidal toxicity of goat weed, Ageratum conyzoides, Linn. (Asteraceae) against weevil, Dermestes maculatus, Degeer (Coleoptera: Dermestidae) infesting smoked fish. Jordan Journal of Biological Sciences, 11, 2. Recovered from: https://jjbs.hu.edu.jo/files/v11n2/Paper%20Number%2015.pdf

Ekundayo, O., Laakso, I., Oguntimein, B., Okogun, J. L., Elujoba, A., & Hulbinen, A. (1987). Essential oil of Ocimum basilicum from Nigeria. Acta Pharmaceutica Fennica, 96, 101-06.

Esper, R. H., Gonçalez, E., Felicio, R. C., & Felicio, J. D. (2015). Fungicidal activity and constituents of Ageratum conyzoides essential oil from three regions in São Paulo state, Brazil. Arquivos do Instituto Biológico, 82, 1-4. Recovered from: https://doi.org/10.1590/1808-1657000482013

Erida, G., Ichsan, C. N., Kurniawan, T., Khan, I. H., & Javaid, A. (2023). Potential of secondary metabolites of Ageratum conyzoides L. in weed management: A review. Allelopathy Journal, 58(1). Recovered from: https://doi.org/10.26651/allelo.j/2023-58-1-1417

Farmacopeia Brasileira (2010). Brasília, Distrito Federal: Agência Nacional de Vigilância Sanitária.

Ferreira, G. F. P., Novaes, Q. S. D., Batista, L. R., Souza, S. E. D., Azevedo, G. B. D., & Silva, D. M. D. (2011). Fungos associados a grãos de café (Coffea arabica L.) beneficiados no sudoeste da Bahia. Summa phytopathologica, 37, 98-102. Recovered from: https://doi.org/10.1590/S0100-54052011000300003

Hussain, A. I., Anwar, F., Nigam, P. S., Ashraf, M., & Gilani, A. H. (2010). Seasonal variation in content, chemical composition and antimicrobial and cytotoxic activities of essential oils from four Mentha species. Journal of the Science of Food and Agriculture, 90(11), 1827-1836. Recovered from: https://doi.org/10.1002/jsfa.4021

Jiang, J., & Xiong, Y. L. (2016). Natural antioxidants as food and feed additives to promote health benefits and quality of meat products: A review. Meat science, 120, 107-117. Recovered from: https://doi.org/10.1016/j.meatsci.2016.04.005

Jomba, T., & Bharali, M. K. (2023). Relative influence of Ageratum conyzoides L. plant extracts on testosterone induced benign prostatic hyperplasia in mice. Pharmacognosy Research, 15(1), 75-83. Recovered from: https://doi.org/10.5530/097484900266

Joshi, R. K. (2014). 6-Demethoxy ageratochromene (Precocene I) rich essential oil of Ageratum conyzoides L. from Western Ghats region of north west Karnataka, India. Journal of Essential Oil-Bearing Plants, 17(3), 422-426. Recovered from: https://doi.org/10.1080/0972060X.2014.884775

Kafi-Farashah, F., Farazmand, H., Vafaei-Shoushtari, R., Golmohammadi, G., & Sanatgar, E. (2018). Effects of anti-juvenile hormone, precocene-I, on egg development of sunn pest, Eurygaster integriceps, and its progenies. Journal of Agricultural Science and Technology, 20(5), 979-986. Recovered from: http://jast.modares.ac.ir/article-23-20336-en.html

Karayat, M., Pal, A. K., Chawla, G., Tolia, A., & Mandal, J. (2024). A review on coacgulation activity of Ageratum conyzoides plant. Public Health, 4, 21-32. Recovered from: https://primerascientific.com/pdf/psmph/PSMPH-04-121.pdf

Kasali, A. A., Winterhalter, P., Adio, A. M., Knapp, H., & Bonnlander, B. (2002). Chromenes in Ageratum conyzoides L. Flavour and Fragrance Journal, 17(4), 247-250. Recovered from: https://doi.org/10.1002/ffj.1099

Kaur, A., Kaur, S., Singh, H. P., Datta, A., Chauhan, B. S., Ullah, H., Kohli, R. K., & Batish, D. R. (2023). Ecology, biology, environmental impacts, and management of an agro-environmental weed Ageratum conyzoides. Plants, 12(12), 2329. Recovered from: https://doi.org/10.3390/plants12122329

Kouame, B. K. F. P., Toure, D., Kablan, L., Bedi, G., Tea, I., Robins, R., Chalchat, J. C., & Tonzibo, F. (2018). Chemical constituents and antibacterial activity of essential oils from flowers and stems of Ageratum conyzoides from Ivory Coast. Records of Natural Products, 12(2), 160-168. Recovered from: http://doi.org/10.25135/rnp.22.17.06.040

Kouassi, K. H. S.; Bajji, M., & Jijakli, H. (2012). The control of postharvest blue and green molds of citrus in relation with essential oil–wax formulations, adherence and viscosity. Postharvest Biology and Technology, 73, 122-128. Recovered from: https://doi.org/10.1016/j.postharvbio.2012.06.008

Linde, G. A., Colauto, N. B., Albertó, E., & Gazim, Z. C. (2016). Quimiotipos, extracción, composición y aplicaciones del aceite esencial de Lippia alba. Revista Brasileira de Plantas Medicinais, 18(1), 191-200. Recovered from: https://doi.org/10.1590/1983-084X/15_037

Liu, X. C., & Liu, Z. L. (2014). Evaluation of larvicidal activity of the essential oil of Ageratum conyzoides L. aerial parts and its major constituents against Aedes albopictus. Journal of Entomology and Zoology Studies, 2(4), 345-350.

Recovered from: https://www.entomoljournal.com/archives/2014/vol2issue4/PartG/94-953.pdf

Makhamra, JM. (2023). Biological Activities and Nutritional Composition of Essential Oil from Rosmarinus officinalis L. Leaves. College of Graduate Studies and Academic Research. Hebron University, 126p. Recovered from: http://dspace.hebron.edu:8080/xmlui/handle/123456789/1337

Ming, L. C. (1999). Ageratum conyzoides: A tropical source of medicinal and agricultural products. Perspectives on new crops and new uses, 1988, 469-473. Recovered from: https://citeseerx.ist.psu.edu/document?repid=rep1&type=pdf&doi=e77b728ad75b7330ac1c55fde828912fa526deb8

NCCLS. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard—Second Edition. NCCLS document M27-A2 (ISBN 1-56238-469-4). NCCLS, 940 West Valley Road, Suite 1400, Wayne, Pennsylvania 19087-1898 USA, 2002.

Nemeth, E., & Bernath, J. (2008). Biological Activities of Yarrow Species (Achillea spp.). Current Pharmaceutical Design, 14(29), 3151-3167. Recovered from: https://doi.org/10.2174/138161208786404281

Oyetayo, V. O. (2009). Free radical scavenging and antimicrobial properties of extracts of wild mushrooms. Brazilian Journal of Microbiology, 40, 380-386. Recovered from: https://doi.org/10.1590/S1517-83822009000200031

Padalia, R. C.; Verma, R. S., & Sundaresan, V. (2010). Volatile constituents of three invasive weeds of Himalayan Region. Records of Natural Products, 4(2), 109-114. Recovered from: https://www.acgpubs.org/RNP/2010/Volume%204/Issue%201/13_RNP-0911-156.pdf

Patil, R. P., Nimbalkar, M. S., Jadhav, U. U., Dawkar, V. V., & Govindwar, S. P. (2010). Antiaflatoxigenic and antioxidant activity of an essential oil from Ageratum conyzoides L. Journal of the Science of Food and Agriculture, 90(4), 608-614. Recovered from: https://doi.org/10.1002/jsfa.3857

Pereira, P., Cebola, M. J., Oliveira, M. C., & Bernardo-Gil, M. G. (2016). Supercritical fluid extraction vs conventional extraction of myrtle leaves and berries: Comparison of antioxidant activity and identification of bioactive compounds. The Journal of Supercritical Fluids, 113, 1-9. Recovered from: https://doi.org/10.1016/j.supflu.2015.09.006

Pérez-Jiménez, J., Arranz, S., Tabernero, M., Díaz-Rubio, M. E., Serrano, J., Goñi, I., & Saura-Calixto, F. (2008). Updated methodology to determine antioxidant capacity in plant foods, oils and beverages: Extraction, measurement and expression of results. Food Research International, 41(3), 274-285. Recovered from: https://doi.org/10.1016/j.foodres.2007.12.004

Powo (2023). Plants of the World Online. Facilitated by the Royal Botanic Gardens, Kew. Published on the Internet. 2023. Accessed date april 11, 2023. Recovered from: https://powo.science.kew.org/

Quoc, T., & Pham, L. (2020). Physicochemical properties and antibacterial activity of essential oil of Ageratum conyzoides L. leaves. Agriculturae Conspectus Scientificus, 85(2), 139-144. Recovered from: https://hrcak.srce.hr/237841

Rahaman, M. M., Hossain, R., Herrera‐Bravo, J., Islam, M. T., Atolani, O., Adeyemi, O. S., Owolodun, O. A., Kambizi, L., Daştan, S. D., Calina, D., & Sharifi‐Rad, J. (2023). Natural antioxidants from some fruits, seeds, foods, natural products, and associated health benefits: An update. Food science & nutrition, 11(4), 1657-1670. Recovered from: https://doi.org/10.1002/fsn3.3217

Riaz, M.; Khalid, M. R., & Chaudhar, F. M. (1995). Essential oil composition of Pakistani Ageratum conyzoides L. Journal of Essential Oil Research, 7, 551-553. Recovered from: https://doi.org/10.1080/10412905.1995.9698584

Rufino, M. S. M., Alves, R. E., Brito, E.S., Morais, S. M., Sampaio, C. G., Pérez- Jiménez, J., & Saura- Calixto, F.D. (2006). Metodologia Científica: Determinação da Atividade Antioxidante Total em Frutas pelo Método de Redução do Ferro (FRAP). Comunicado Técnico Embrapa, 125(1), 1-4. Recovered from: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/664098

Rufino, M. D. S. M., Alves, R. E., de Brito, E. S., de Morais, S. M., Sampaio, C. D. G., Pérez-Jimenez, J., & Saura-Calixto, F. D. (2007). Metodologia Científica: determinação da atividade antioxidante total em frutas pela captura do radical livre DPPH. Comunicado Técnico Embrapa, 127, 1-4. Recovered from: https://ainfo.cnptia.embrapa.br/digital/bitstream/CNPAT/10224/1/Cot_127.pdf

Rufino, M. S. M., Alves, R. E., Brito, E. S., Pérez- Jiménez, J., Saura- Calixto, F. D., & Mancini- Filho, J. (2010). Bioactive compounds and antioxidant capacities of 18 non-traditional tropical fruits from Brazil. Food Chemistry, 121, 996-1002.

Recovered from: https://doi.org/10.1016/j.foodchem.2010.01.037

Ruberto, G., & Baratta, M. T. (2000). Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69(2), 167–174. Recovered from: https://doi.org/10.1016/S0308-8146(99)00247-2

Rossato Viana, A., Godoy Noro, B., Santos, D., Wolf, K., Sudatti Das Neves, Y., Moresco, R. N., Ourique, A. F., Flores, E. M. M., Rhoden, C. R. B., Krause, L. M. F., & Stefanello Vizzotto, B. (2023). Detection of new phytochemical compounds from Vassobia breviflora (Sendtn.) Hunz: antioxidant, cytotoxic, and antibacterial activity of the hexane extract. Journal of Toxicology and Environmental Health, Part A, 86(2-3), 51-68. Recovered from: https://doi.org/10.1080/15287394.2022.2156956

Sastry, K.S., Mandal, B., Hammond, J., Scott, S.W., & Briddon, R.W. (2019). Ageratum spp. (White weed). Encyclopedia of Plant Viruses and Viroids, New Delhi: Springer. Recovered from: https://doi.org/10.1007/978-81-322-3912-3_20

Satija, S., Kamboj, S., Kaur, J., Mahajan, S., Neeta., Sharma, N., Khurana, N., Vyas, M., & Mehta, M. (2018). Morphological and Anatomical Studies of Stem of Ageratum conyzoides Saurabh. International Journal of Green Pharmacy, 127, 127-130. Recovered from: https://doi.org/10.22377/ijgp.v12i02.1842

Sivakumar, D., & Bautista-Baños, S. A. (2014). Review on the use of essential oils for postharvest decay control and maintenance of fruit quality during storage. Crop Protection, 64, 27–37. Recovered from: https://doi.org/10.1016/j.cropro.2014.05.012

Viana, A. R., Bottari, N. B., Oviedo, V. R., Santos, D., Londero, J. E. L., Schetinger, M. R. C., Flores, E.M.M., Pigatto, A., Schuch, A. P., Krause, A., & Krause, L. M. F. (2023). Phytochemical and biological characterization of aqueous extract of Vassobia breviflora on proliferation and viability of melanoma cells: involvement of purinergic pathway. Journal of Toxicology and Environmental Health, Part A. 86(17), 632-652. Recovered from: https://doi.org/10.1080/15287394.2023.2233989

Yadav, N., Ganie, S. A., Singh, B., Chhillar, A. K., & Yadav, S. S. (2019). Phytochemical constituents and ethnopharmacological properties of Ageratum conyzoides L. Phytotherapy Research, 33(9), 2163-2178. Recovered from: https://doi.org/10.1002/ptr.6405

Zoghbi, M. D. G., Bastos, M. D. N. D. C., Jardim, M. A. G., & Trigo, J. R. (2007). Volatiles of inflorescences, leaves, stems and roots of Ageratum conyzoides L. growing wild in the north of Brazil. Journal of Essential Oil-Bearing Plants, 10(4), 297-303. Recovered from: https://doi.org/10.1080/0972060X.2007.10643558

Publicado

2024-11-29

Como Citar

Pereira, V. S. R., Silva, G. C. C., Oliveira, E. B. da C., Machado, M. de A., Sena, J. da S., Bento, M. C. V. de A., Rodrigues, S. A., Inumaro, R. S., Gonçalves, J. E., Nunes, M. G. I. F., Herrig, S. P. R., Piau Junior, R., & Gazim, Z. C. (2024). Ageratum conyzoides, uma espécie invasiva com potencial antioxidante e antifúngica. Ciência E Natura, 46, e83759. https://doi.org/10.5902/2179460X83759

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Química

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