Chemical profile analysis and therapeutic potential of Caiman yacare paracloacal gland oil ethanolic extract (Daudin, 1802)
DOI:
https://doi.org/10.5902/2179460X85626Keywords:
Cytotoxicity, Antimicrobial, Chemical compositionAbstract
With the aim of expanding our knowledge of the Caiman yacare paracloacal gland oil ethanolic extract (Daudin, 1802), we investigated the chemical composition and antimicrobial activity of the ethanolic extract as well as its influence on cell viability. Fourier transform infrared spectroscopy and gas chromatography coupled with mass spectrometry were used to determine the chemical profile. Subsequently, using the broth microdilution method, we evaluated the ethanolic extract for its antimicrobial effect against 15 bacterial strains and two yeast strains as well as its effect on cell viability in different glial cell and skin tissue lineages using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The ethanolic extract was found to have a heterogeneous composition, containing compounds such as nitrosourea, hydrocarbons, ketones, and esters. Additionally, the ethanolic extract exhibited a bacteriostatic effect at concentrations above 200 µg/mL against Pseudomonas aeruginosa and above 400 µg/mL against Streptococcus pyogenes and Enterococcus faecalis. The ethanolic extract reduced cell viability only at the two highest concentrations (500 and 1000 µg/mL) in fibroblasts (GM07492) and at the highest concentration in astrocytes (ACBRI-371) and glioblastoma multiforme (T98G). These findings contribute to a better understanding of the pharmacological potential of C. yacare paracloacal gland oil, providing support for future investigations and the development of therapeutic products.
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References
Alves, R. R., & Alves, H. N. (2011). The faunal drugstore: Animal-based remedies used in traditional medicines in Latin America. Journal of Ethnobiology and Ethnomedicine, 7(1), 9-7. https://doi.org/10.1186/1746-4269-7-9
Alves, R. R. N., Vieira, W. L. S., & Santana, G. G. (2008). Reptiles used in traditional folk medicine: conservation implications. Biodiversity and Conservation, v 17(8), 2037–2049. https://doi.org/10.1007/s10531-007-9305-0
Aslantürk, Ö. S. (2018). In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages. In Larramendy, M. L., & Soloneski, S. (Eds.), Genotoxicity - A Predictable Risk to Our Actual World (pp. 1-18). InTechOpen. https://doi.org/ 10.5772/intechopen.71923
Azevedo, L. P., Pressinotti, L. N., Liotti, R. G., Galbiati, Loureiro, E. M., Pinto, C. L., Lima, N. R. de O., & Aleixo, M. L. M. (2018). Antifungal activity of paracloacal gland secretion of Caiman yacare (DAUDIN, 1802). Revista Ibero-Americana de Ciências Ambientais, 9(1), 140–151. https://doi.org/10.6008/cbpc2179-6858.2018.001.0010
Azevedo, L. P., Ferraz, R. H. dos S., Magalhães, M. R. L., Oliveira, A. P., Cogliati, B., Lemos, L. M. S., Branco, P. C., & Pressinotti, L. N. (2020). Healing potential of Caiman yacare (Daudin, 1802) visceral fat oil. Wound Medicine, 31(June), 100195. https://doi.org/10.1016/j.wndm.2020.100195
Barzilai, A., & Yamamoto, K.-I. (2004). DNA damage responses to oxidative stress. DNA Repair, 3(8–9), 1109–1115. https://doi.org/ 10.1016/j.dnarep.2004.03.002
Brandes, A. A., Bartolotti, M., Tosoni, A., & Franceschi, E. (2016). Nitrosoureas in the Management of Malignant Gliomas. Current Neurology and Neuroscience Reports, 16(2), 1–7. https://doi.org/10.1007/s11910-015-0611-8
Buthelezi, S., Southway, C., Govinden, U., Bodenstein, J., & Toit, K. (2012). An investigation of the antimicrobial and anti-inflammatory activities of crocodile oil. Journal of Ethnopharmacology, 143(1) 325–330. https://doi.org/10.1016/j.jep.2012.06.040
Campos, Z., & Coutinho, M. (2006). Sistema de criação e recria de jacaré, Caiman crocodilus yacare, no Pantanal (Comunicado Técnico, 53). Embrapa Pantanal. https://www.embrapa.br/busca-de-publicacoes/-/publicacao/812569/sistema-de-criacao-e-recria-de-jacare-caiman-crocodilus-yacare-no-pantanal
Cardoso, C. R. B., Souza, M. A., Ferro., E. A. V., Favoretto, S., & Pena, J. D. O. (2004). Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acids on the healing of cutaneous wounds. Wound Repair and Regeneration, 12(2), 235–243. https://doi.org/10.1111/j.1067-1927.2004.012216.x
Carreira, L. B. T., & Sabbag, O. J. (2015). Economic aspects of production of Caiman crocodilus yacare. Anais da Academia Brasileira de Ciencias, 87(1), 495–502. https://doi.org/10.1590/0001-3765201520130286
Clinical and Laboratory Standards Institute. (2008). Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved standard—Third edition (CLSI document M27-A3). Wayne, PA: CLSI.
Dehaan, C., Habibi-Nazhad, B., Yan, E., Salloum, N., Parliament, M., & Allalunis-Turner, J. (2004). Mutation in mitochondrial complex I ND6 subunit is associated with defective response to hypoxia in human glioma cells. Molecular Cancer, 3, 1–15. https://doi.org/10.1186/1476-4598-3-19
Dunn, B. S. J. Weldon, P. J., Howard, R. W., & McDaniel, C. A. (1993). Lipids From the Paracloacal Glands of the Chinese Alligator (Alligator sinensis). American Oil Chemist’s Society, 28(1), 75–78. https://doi.org/10.1007/BF02536366
Eloff, J. (1998). A Sensitive and Quick Microplate Method to Determine the Minimal Inhibitory Concentration of Plant Extracts for Bacteria. Planta Medica, 64(8), 711–713. https://doi.org/10.1055/s-2006-957563
Hood, J. R., Wilkinson, J. M., & Cavanagh, H. M. A. (2003). Evaluation of common antibacterial screening methods utilized in essential oil research. Journal of Essential Oil Research, 15(6), 428–433. https://doi.org/10.1080/10412905.2003.9698631
Hyldgaard, M., Mygind, T., & Meyer, R. L. (2012). Essential oils in food preservation: Mode of action, synergies, and interactions with food matrix components. Frontiers in Microbiology, 3(Jan), 1–24. https://doi.org/10.3389/fmicb.2012.00012
Javidnia, K., Parish, M., Karimi, S., & Hemmateenejad. (2013). Discrimination of edible oils and fats by combination of multivariate pattern recognition and FT-IR spectroscopy: A comparative study between different modeling methods. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 104, 175–181. https://doi.org/10.1016/j.saa.2012.11.067
Junker, J. P., & Van Oudenaarden, A. (2014). Every cell is special: Genome-wide studies add a new dimension to single-cell biology. Cell, 157(1), 8–11. https://doi.org/10.1016/j.cell.2014.02.010
Kastaniotis, A. J., Autio., K. J., Kerätär, J. M., Monteuuis, G., Mäkelä, A. M., Nair, R. R., Pietikäinen., L. P., Shvetsova, A., Chen, Z., & Hiltunen, J. K. (2017). Mitochondrial fatty acid synthesis, fatty acids and mitochondrial physiology. Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1862(1), 39–48. https://doi.org/10.1016/j.bbalip.2016.08.011
Kumar, P., Nagarajan, A., & Uchil, P. D. (2018). Analysis of Cell Viability by the MTT Assay. Cold Spring Harbor Protocols, 2018(6), 469–471. https://doi.org/10.1101/pdb.prot095505
Leipnitz, G., Mohsen, Al-W., Karunanidhi, A., Seminotti, B., Roginskaya, V. Y., Markantone, D. M., Grings., M., Mihalik., S. J., Wipf, P., Houten, B. V., & Vockley, J. (2018). Evaluation of mitochondrial bioenergetics, dynamics, endoplasmic reticulum-mitochondria crosstalk, and reactive oxygen species in fibroblasts from patients with complex i deficiency. Scientific Reports, 8(1), 1–14. https://doi.org/10.1038/s41598-018-19543-3
Leiva, P. M. L., Valli, F. E., Piña, C. I., González., M. A., & Simoncini, M. (2022). Chemical characterization and potential use of reptile fat from sustainable programs. Ethnobiology and Conservation, 6(March), 11. https://doi.org/10.15451/ec2022-03-11.06-1-12
Li, H.-L., Liu, X. -T., Huang, S. -M., Xiong, Y. -X., Zhang., Z. -R., Zheng, Y. -H., Chen, Q. -X., & Chen, Q. -H. (2021). Repair function of essential oil from Crocodylus Siamensis (Schneider, 1801) on the burn wound healing via up-regulated growth factor expression and anti-inflammatory effect. Journal of ethnopharmacology, 264(June), 113286. https://doi.org/10.1016/j.jep.2020.113286
Mbah, J. A., Ngemenya, M. N., Abawah, A. L., Babiaka, S. B., Nubed, L. N., Nyongbela, K. D., Lemuh, N. D., & Efange, S. M. N. (2012). Bioassay-guided discovery of antibacterial agents: In vitro screening of Peperomia vulcanica, Peperomia fernandopoioana and Scleria striatinux. Annals of Clinical Microbiology and Antimicrobials, 11, 1–10. https://doi.org/10.1186/1476-0711-11-10
Medeiros, M. F. T., & Alves, R. R. N. (2020). Nineteenth century zootherapy in benedictine monasteries of brazil. Anais da Academia Brasileira de Ciências, 92(2), 1–11. https://doi.org/10.1590/0001-3765202020181113
Morinet, F. (2013). Fond génétique et maladies infectieuses. Pathologie Biologie, 61(1), 1–2. https://doi.org/10.1016/j.patbio.2013.01.006
Ngernjan, M., Ontawong, A., Lailerd, N., Mengamphan, K., Sarapirom, S., & Amornlerdpison, D. (2022). Crocodile Oil Modulates Inflammation and Immune Responses in LPS-Stimulated RAW 264.7 Macrophages. Molecules, 27(12), 3784. https://doi.org/10.3390/molecules27123784
Nováková, L., Dousa, M., Pekarek, T., & Mitasik, L. (2019). Pharmaceutical analysis: overview. Encyclopedia of Analytical Science, 8(September), 200–218. https://doi.org/ 10.1016/B978-0-12-409547-2.14504-4
OECD. (2014). Test No. 487: In Vitro Mammalian Cell Micronucleus Test. In Oecd Guideline for the Testing of Chemicals, Section 4. (Issue September, pp. 1–21). https://doi. org/10.1787/9789264224292-en
Rohman, A., Sismindari., Erwanto, Y., & Man, Y. B. M. (2011). Analysis of pork adulteration in beef meatball using Fourier transform infrared (FTIR) spectroscopy. Meat Science, 88(1), https://doi.org/91–95. 10.1016/j.meatsci.2010.12.007
Santos, A. L., & Preta, G. (2018). Lipids in the cell: organisation regulates function. Cellular and Molecular Life Sciences, 0123456789, 1–19. https://doi.org/10.1007/s00018-018-2765-4
Schmeda-Hirschmann, G., Delporte, C., Valenzuela-Barra, G., Silva, X., Vargas-Arana, G., Lima, B., & Feresin, G. E. (2014). Anti-inflammatory activity of animal oils from the Peruvian Amazon. Journal of Ethnopharmacology, 156, 9–15. https://doi.org/10.1016/j.jep.2014.08.010
Shim-Prydon, G. and Camacho-Barreto, H (2007). New animal products: new uses and markets for co/by-products of crocodile, emu, goat, kangaroo and rabbit.
Singh, D., Kumar, T. R. S., Gupta, V. K., & Chaturvedi, P. (2012). Antimicrobial activity of some promising plant oils, molecules and formulations. Indian Journal of Experimental Biology, 50(10), 714–717.
Vera-Candioti, L., Leiva, P. M. L., Valli, F., Bernal, C. A., Piña, C. I., Simoncini, M. S., & González, M. A. (2021). Optimization of oil extraction from caiman fat. Characterization for use as food supplement. Food Chemistry, 357(March), 129755. https://doi.org/10.1016/j.foodchem.2021.129755
Wald-Dickler, N., Holtom, P., & Spellberg, B. (2018). Busting the Myth of “Static vs Cidal”: A Systemic Literature Review. Clinical infectious diseases, 66(9), 1470–1474. https://doi.org/10.1093/cid/cix1127
Weldon, P. J., & Sampson H. W. (1987). Paracloacal glands of Alligator mississippiensis: A histological and histochemical study. Journal of zoology, 212, 109–115. https://doi.org/10.1111/j.1469-7998.1987.tb05118.x
Weldon, P. J., Scott, T. P., & Tanner, M. J. (1990). Analysis of Gular and Paracloacal Gland secretions of the American Alligator (Alligar mississippiensis) by Thin-Layer Chromatography. Journal of chemical ecology, 16(1), 3–12. https://doi.org/10.1007/BF01021263
Weldon, P. J., Shafagati, A., Wheeler, J. W. (1989). Lipids on the paracloacal gland secretions of Dwarf (Paleosuchus palpebrosus) and Smooth-fronted (P.trigonatus) Caimans. Biochemical Systematics and Ecology, 17(5), 431–435. https://doi.org/10.1016/0305-1978(89)90061-6
Wymann, M. P., & Schneiter, R. (2008). Lipid signalling in disease. Nature Reviews Molecular Cell Biology, 9(2), 162–176. https://doi.org/10.1038/nrm2335
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