Essential oils against gastrointestinal nematodes in sheep in vitro and chemical composition of those plants

Authors

DOI:

https://doi.org/10.5902/2179460X71665

Keywords:

Anthelmintic, Sheep production, Worms

Abstract

The present study aimed to evaluate the in vitro potential of aromatic plants Aloysia triphylla (cidró ou erva-luísa), Corymbia citriodora (eucalipto-limão), Lippia alba (falsa-melissa) e Piper gaudichaudianum (pariparoba), in helminths eggs and larvae, determine their main constituents. For this, the essential oils (OEs) of the plants were extracted from leafs, in the clevenger apparatus and to determine the main constituents was carried out the gas chromatography coupled to mass spectrometry. The Egg eclodibility assay (EEA) and Larval development test (DLT) were performed using essential oils at concentrations ranged from 75 at 0.2%. mg.mL, and all tests followed by commercial anthelmintic positive control and negative control distilled water. For EEA, A. triphylla e L. alba OEs demonstred equivalente efficacy to the commercial drug at all concentrations that was tested, while P. gaudichaudianum OE demonstred equivalente efficacy at 15 mg/mL and the C. citriodora OE at 10 mg/mL. About DLT, all concentratios of the studied essential oils were effective as the comercial drug results in this test. The dominant constituent in OEs were: geraniale (A. triphylla), citronellal (L. alba), linalool (L. alba) and α-humuleno (P. gaudichaudianum). Based on the results presented here, the OEs present potential for use as natural anthelmintics for the treatment of endoparasitosis in sheep.

Downloads

Download data is not yet available.

Author Biographies

Thaís Moreira Osório, Universidade Estadual do Rio Grande do Sul

She has a degree in Biological Sciences from the Fundação Universidade Regional de Blumenau (2008), a master's degree in Biotechnology from the Federal University of Santa Catarina (2011) and a doctorate in Biological Sciences from the Federal University of Pampa (2022). Currently works as a technical laboratory agent at the State University of Rio Grande do Sul. Approved and invited to the competition for substitute professor of Biology at Ifsul Santana do Livramento.

Leonardo de Melo Menezes, Universidade Estadual do Rio Grande do Sul

Veterinary Doctor from the Federal University of Pelotas, Specialist in Business Administration from Fundação Getúlio Vargas - FGV/RJ, Master in Animal Science and PhD in Animal Science from the University Federal of Pelotas. Graduating in Economic Sciences from the Federal University of Pampa. He is currently a professor at the State University of Rio Grande do Sul for Agronomy and Rural Development courses at the Santana do Livramento. Editor of the UERGS Scientific Journal. Research group leader registered with Cnpq (GEPAFO - Study Group on Livestock and Agriculture of the Western Frontier). He has experience in Animal Production, with an emphasis on the production of beef cattle and sheep, working on the following topics: milk production, production systems, animal behavior, vermin control, technical-economic efficiency, management of production.

Anelise Affonso Martins, Universidade Federal do Pampa

Graduated in Veterinary Medicine from the Federal University de Pelotas, master's degree in Veterinary Science from the Federal University of Pelotas and PhD in Veterinary Sciences from the Federal University of Rio Grande do Sul. Veterinary doctor at the Federal University of Pampa since 2010. Has experience in the field of Veterinary Medicine, with an emphasis on Veterinary Medicine Preventive, working mainly on the following topics: Microbiology - diseases fungal and bacterial; Parasitology - gastrointestinal parasites of ruminants and monogastric.

Denise Schimidt, Universidade Federal de Santa Maria

He has a degree in Agronomy from the Federal University of Santa Maria, a master's degree in Agronomy from the Federal University of Santa Maria and a PhD in Agronomy from the Federal University of Santa Maria. She is currently a Full Professor at the Federal University of Santa Maria. Scholarship in 1D productivity from CNPq. He has experience in the area of ​​Agronomy, with an emphasis on horticulture, working mainly on the following topics: vegetables, protected environment, aromatic plants and essential oils.

Matheus Milani Pretto, Universidade Estadual Paulista (Unesp)

Agricultural Engineer and Master in Agronomy from the Federal University of Santa Maria campus Frederico Westphalen. He is currently a PhD student in Plant Protection at the São Paulo State University "Júlio de Mesquita Filho". He operates in the following areas: Cultivation of aromatic species, extraction of essential oils, alternative control, phytopathology, nematology, development of products to control pests and agricultural diseases. Member of the Agricultural Nematology and Vegetable Genetic Improvement research groups.

Karoline Barcellos da Rosa, Universidade Federal de Pelotas

Agricultural Engineer from the State University of Rio Grande do Sul, Master's student in Animal Science from the Federal University of Pelotas. Area of ​​concentration in animal production.

Velci Queiróz de Souza, Universidade Federal do Pampa

He completed his post-doctorate in agronomy at the Federal University of Pelotas in 2008 - area of ​​concentration in plant genetic improvement. He is currently coordinator of technological innovation at the Federal University of Pampa. He works in the area of ​​agronomy, with an emphasis on experimental statistics and genetic improvement. In his lattes curriculum, the most frequent terms in the context of scientific and technological production are: plant genetic improvement, experimental statistics, progeny selection, genotype and environment interaction, and resistance to biotic and abiotic factors.

References

ABBAS, R. Z.; ZAMAN, M. A.; SINDHU, Z. U. D.; SHARIF, M.; RAFIQUE, A.; SAEED, Z.; REHMAN, T. U.; SIDDIQUE, F.; ZAHEER, T.; KHAN, M. K. Anthelmintic effects and toxicity analysis of herbal dewormer against the infection of Haemonchus contortus and Fasciola hepatica in goat. Pakistan Veterinary Journal 40:455–460, 2020. DOI: 10.29261/pakvetj/2020.083.

AMARANTE, AFT. Classe nematoda. In: Os parasitas de ovinos [online]. São Paulo: Editora UNESP, 2014, pp. 13-97. ISBN 978-85-68334-42-3. Disponível em: https://books.scielo.org/id/nv4nc/pdf/amarante-9788568334423-03.pdf

ASSIS, L. M.; BEVILAQUA, C. M. L.; MORAIS, S. M.; VIEIRA, L.S.; COSTA, C. T. C.; SOUZA, J. A. L. Ovicidal and larvicidal activity in vitro of Spigelia anthelmia Linn. extracts on Haemonchus contortus. Ciência Animal, v. 9, p. 67-73, 1999. DOI: 10.1016/j.vetpar.2003.07.021

ASSIS, L. M.; BEVILAQUA, C. M. L.; MORAIS, S. M.; VIEIRA, L. S.; COSTA, C. T. C.; SOUZA, J. A. L. Ovicidal and larvicidal activity in vitro of Spigelia anthelmia Linn. extracts on Haemonchus contortus. Veterinary Parasitology, Netherlands, v. 117, p. 43-49, 2003. DOI: https://doi.org/10.1016/j.vetpar.2003.07.021.

BAHL, L. R.; GARG, S. N.; SINGH, S.; BANSAL, R. P.; NAQVI, A. Composition of linalool rich essential oil from Lippia alba grown in Indian plants. Flavour and Fragrance Journal, v.15, n.3, p.199-200, 2000. DOI: https://doi.org/10.1002/1099-1026(200005/06)15:3<199::AID-FFJ892>3.0.CO;2-S

BELECKĖ, A.; KUPČINSKAS, T.; STADALIENĖ, I.; HÖGLUND, J.; THAMSBORG, S. M.; STUEN, S.; PETKEVIČIUS, S. Anthelmintic resistance in small ruminants in the Nordic-Baltic region. Acta Veterinaria Scandinavica 63:18, 2021. DOI: https://doi.org/10.1186/s13028-021-00583-1.

BEVILAQUA, C.M.L.; VASCONCELOS, A.L.F.; MORAIS, S.M. Ovicidal and larvicidal activity of Lippia sidoides and Ocimum gratissimum essencial oils against Haemonchus contortus. Proceedings oft the 20th International conference of the World Association for the Advancement of Veterinary Parasitology, Christchurch. New Zealand Veterinary Journal, v.1, p.78-9, 2005.

BIZIMINYERA, E. S.; GITHIORI, J. B.; ELOFF, J. N.; SWAN, G. E. In vitro activity of Peltophorum africanum Sond. (Fabacea) extracts on the egg hatching and larval development of the parasitic nematode Trichostrongylus colubriformis. Veterinary Parasitology, v.142, p.336-343, 2006. DOI: https://doi.org/10.1016/j.vetpar.2006.06.013.

BLANCO, M. A.; COLAREDA, G. A.; VAN BAREN, C.; BANDONI, A. L.; RINGUELET, J.; CONSOLINI, A. E. Antispasmodic effects and composition of the essential oils from two South American chemotypes of Lippia alba. Journal of Ethnopharmacology, v. 149, n. 3, p. 803-809, 2013. DOI: https://doi.org/10.1016/j.jep.2013.08.007

BOSCO, A.; KIEßLER, J.; AMADESI, A.; VARADY, M.; HINNEY, B.; IANNIELLO, D.; PAOLA MAURELLI, M.; CRINGOLI, G.; RINALDI, L. The

threat of reduced efficacy of anthelmintics against gastrointestinal nematodes in sheep from an area considered anthelmintic resistance-free. Parasit. Vectors. 13:457, 2020. DOI: https://doi.org/10.1186/s13071-020-04329-2.

CAMARGO, S. B.; DE VASCONCELOS, D. F. S. A. Atividades biológicas de Linalol: conceitos atuais e possibilidades futuras deste monoterpeno. Revista de Ciências Médicas e Biológicas, v. 13, n. 3, p. 381-387, 2015. DOI: 10.9771/cmbio.v13i3.12949.

CAMURÇA-VASCONCELOS, A. L. F.; BEVILAQUA, C. M. L.; MORAIS, S. M.; MACIEL, M. V.; COSTA, C. T.; MACEDO, I. T.; OLIVEIRA, L. M.; BRAGA, R. R.; SILVA, R. A.; VIEIRA, L. S. Anthelmintic activity of Croton zehntneri and Lippiasidoides essential oils. Veterinary Parasitology, v. 148, p. 288-294, 2007. DOI: https://doi.org/10.1016/j.vetpar.2007.06.012.

CASTRO, L. M.; PINTO, N. B.; MOTA, T. O.; MOURA, M. Q.; DIAS DE CASTRO, L. L.; MADRID, I. M.; FREITAG, R. A.; BERNE, M. E. A. Atividade ovicida do óleo essencial e do extrato hidroalcoólico de Ocimum basilicum sobre nematódeos gastrintestinais de ovinos. Science And Animal Health, vol. 5, n. 2, p. 138-150, 2017. DOI: http://dx.doi.org/10.15210/sah.v5i2.10726

CHAGAS, A. C. S.; PASSOS, W. M.; PRATES, H. T.; LEITE, R. C.; FURLONG, J.; FORTES, I. C. P. Efeito acaricida de óleos essenciais e concentrados emulsionáveis de Eucalyptus spp em Boophilus microplus. Brazilian Journal of Veterinary Research and Animal Science, v.39, n.5, p.247-253, 2002. DOI: https://doi.org/10.1590/S1413-95962002000500006. Disponível em: https://www.scielo.br/j/bjvras/a/xLRwkPsKqSs9hML7kxMTy5s/?format=pdf&lang=pt. Acesso em: 10 ago. 2022.

CHAGAS, A. C. de S.; NICIURA, S. C. M.; MOLENTO, M. B (Ed.). Manual prático: metodologias de diagnóstico da resistência e de detecção de substâncias ativas em parasitas de ruminantes. Brasília, DF: Embrapa Informação Tecnológica; São Carlos, SP: Embrapa Pecuária Sudeste, 153 p., 2011.

COLES, G.C.; BAUER, C.; BORGSTEEDE, F. H. M. BORGSTEEDE, F. H.; GEERTS, S.; KLEI, T. R.; TAYLOR, M. A.; WALLER, P. J. World Association for the Advancement of Veterinary Parasitology (WAAVP) - methods for detection of anthelmintic resistance in nematodes of veterinary importance. Veterinary Parasitology, v.44, p.35-44, 1992. DOI: https://doi.org/10.1016/0304-4017(92)90141-U.

COLES, G. C.; JACKSON, F.; POMROY, W. E.; PRICHARD, R. K., SAMSON-HIMMELSTJERNA, G. von; SILVESTRE, A.; TAYLOR, M. A.; VERCUYSSE, J. The detection of anthelmintic resistance in nematodes of veterinary importance. Veterinay Parasitology, 136: 167–85, 2006. DOI: https://doi.org/10.1016/j.vetpar.2005.11.019.

COOP, R. L.; KYRIAZAKIS, I. Influence of host nutrition on the development and consequences of nematode parasitism in ruminants. Trends in Parasitology, v. 17, p. 325-330. 2001. DOI: https://doi.org/10.1016/S1471-4922(01)01900-6

COSTA, C. T. C.; MORAIS, S. M. DE; BEVILAQUA, C. M. L.; SOUZA, M. M. C. DE; LEITA, E. K. A. Efeito ovicida de extratos de sementes de Mangifera indica L. sobre Haemonchus contortus. Revista Brasileira de Parasitologia Veterinária, São Paulo, v. 11, n. 2, p. 57-60, 2002.

COSTA, S. S.; OLIVEIRA, D. B.; MANÇO, A. M.; MELO, G. O. de; CORDEIRO, J. L. P.; ZANIOLO, S.; NEGRELLE, R. P. B.; OLIVEIRA, L. F. B. Plants composing the diet of marsh and pampas deer in the Brazilian Pantanal wetland and their ethnomedicinal properties. J Biol Sci 5, n. 5: 840-846, 2006. DOI: 10.3923/jbs.2006.840.846

CUNHA, M. A.; BARROS, F. M. C.; GARCIA, L. O.; VEECK, A. P. L.; HEINZMANN, B. M.; LORO, V. L.; EMANUELLI, T.; BALDISSEROTTO, B. Essential oil of Lippia alba: A new anesthetic for silver catfish, Rhamdia quelen. Aquaculture, [s.l.], v. 306, n. 1-4, p.403- 406, 2010. DOI: https://doi.org/10.1016/j.aquaculture.2010.06.014

DANIEL, A. P.; VEECK, A. P. L.; KLEIN, B.; FERREIRA, L. F.; CUNHA, M. A.; PARODI, T. V.; ZEPPENFELD, C. C.; SCHMIDT, D.; CARON, B. O.; HEINZMANN, B. M.; BALDISSEROTTO, B.; EMANUELLI, T. Using the essential oil of Aloysia triphylla (L’Her.) Britton to sedate Silver Catfish (Rhamdia quelen) during transport improved the chemical and sensory qualities of the fish during storage in ice. Journal of Food Science, v. 79, n. 6, 2014. DOI: https://doi.org/10.1111/1750-3841.12463

DI STASI, L. C., HIRUMA-LIMA, C. A. Plantas Medicinais na Amazônia e na Mata Atlântica. São Paulo, pp 604, 2022.

FELIX, P. H. C. Microencapsulação de óleo essencial de limão (Citrus aurantifolia): emulsões e estudo das propriedades físicos-químicas. 117 f. Tese (Doutorado) - Curso de Ciência dos Alimentos, Universidade Federal de Lavras, Lavras, 2017. Disponível em: http://repositorio.ufla.br/jspui/bitstream/1/13314/1/TESE_Microencapsula%C3%A7% C3%A3o%20de%20%C3%B3leo%20essencial%20de%20lim%C3%A3o%20%28Citr us%20aurantifolia%29....pdf. Acesso em: 04 jun. 2021.

FIGUEIREDO, R. O.; STEFANINI, M. B.; MING, L. C.; MARQUES, M. O. M.; FACANALI, R. Essential oil composition of Aloysia triphylla (L›Herit) Britton leaves cultivated in Botucatu, São Paulo, Brazil. Acta Horticulturae, 629:131-134, 2004. Disponível em: http://hdl.handle.net/11449/68017.

FORTES F. S.; MOLENTO M. B. Resistência antihelmíntica em nematóides gastrintestinais de pequenos ruminantes: avanços e limitações para seu diagnóstico. Pesquisa Veterinária Brasileira, v. 33, n. 12, p. 1391-1402, 2013. DOI: https://doi.org/10.1590/S0100-736X2013001200001.

GIRÃO, E. S.; MEDEIROS, L. P.; GIRÃO, R. N. Ocorrência e distribuição estacional de helmintos gastrintestinais de caprinos no município de Teresina, Piauí. Ciência Rural, v. 22, p. 197-202. 1992. DOI: https://doi.org/10.1590/S0103-84781992000200013

GIRÃO E.S.; CARVALHO J.H. Avaliação de plantas medicinais com efeito anti-helmíntico em caprinos. Revista CFMV, 5(18):14-16, 1999.

GOBBO-NETO, L.; LOPES, N. P. Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários. Química Nova, v. 30, n. 2, p. 374-381, 2007. DOI: https://doi.org/10.1590/S0100-40422007000200026.

GOMIDE, M. D. S.; LEMOS, F. D. O.; LOPES, M. T. P.; ALVES, T. M. D. A.; VICCINI, L. F.; COELHO, C. M. The effect of the essential oils from five different Lippia species on the viability of tumor cell lines. Revista Brasileira de Farmacognosia, v. 23, n. 6, p. 895-902, 2013. DOI: https://doi.org/10.1590/S0102-695X2013000600006.

GONÇALVES, I. G. de; ECHEVERRIA, F. Cobre no controle da verminose gastrintestinal em ovinos. Ciência Rural, Santa Maria, v. 34, n. 1, p. 183-188, 2004. DOI: https://doi.org/10.1590/S0103-84782004000100028.

GRANDO, T. H; SÁ, M. F de; BALDISSERA, M. D; OLIVEIRA, C. B; SOUZA, M. E de; RAFFIN, R. P; SANTOS, R. C. V; DOMINGUES, R; MINHO,

A. P; LEAL, M. L. R; MONTEIRO, S. G. In vitro activity of essential oils of free and nanostructured Melaleuca alternifolia and of terpinen-4-ol on eggs and larvae of Haemonchus contortus. Journal of Helminthology (2016) 90, 377–382 DOI: http://doi.org/10.1017/S0022149X15000401

HAMMOND, J. A.; FIELDING, D.; BISHOP, S. C. Prospects for plant anthelmintcs in tropical veterinary medicine. Veterinary Research Communications. Netherlands, v. 21, p. 2013-228, 1997. DOI: https://doi.org/10.1023/A:1005884429253.

HOHLENWERGER, J. C.; BALDISSEROTTO, B.; COUTO. R. D.; HEIZMANN, B. M.; SILVA, D. T.; CARON, B. O.; SCHMIDT, D.; COPATTI, C. E. Essential oil of Lippia alba in the transport of Nile tilapia. Ciência Rural, Santa Maria, v. 47, n. 3, p. 1-4, 2017. DOI: https://doi.org/10.1590/0103-8478cr2016 0040. Disponível em: https://www.scielo.br/pdf/cr/v47n3/1678-4596-cr-47-03-e20160040.pdf. Acesso em: 10 nov. 2020.

HOMERO, H. Q. Parasitología y enfermedades parasitarias de Animales Domesticos. Limusa, 1 (1 ed), 1984.

KAMPKE, E. H., BARROSO, M. E. de S., MARQUES, F. M., FRONZA, M., SCHERER, R., LEMOS, M. F., CAMPAGNARO, B. P., GOMES, L. C., Genotoxic effect of Lippia alba (Mill.) N. E. Brown) essential oil on fish (Oreochromis niloticus) and mammal (Mus musculus). Environmental Toxicology and Pharmacology. v. 59. 2018. DOI: https://doi.org/10.1016/j.etap.2018.03.016

KAPLAN, R. M.; VIDYASHANKAR, A. N. An inconvenient truth: Global worming and anthelmintic resistance. Veterinary Parasitology, v. 186, n. 12, p. 70-78, 2012. DOI: https://doi.org/10.1016/j.vetpar.2011.11.048

KETZIS, J. K.; TAYLOR, A.; BOWMAN, D. D.; BROWN, D. L.; WARNICK, L. D.; ERB, H. N. Chenopodium ambrosoides and its essential oil as treatments for Haemonchus contortus and mixed adult-nematode infections in goats. Small Ruminant Research, Amsterdam, v. 44, p. 193-200, 2002. DOI: https://doi.org/10.1016/S0921-4488(02)00047-0

LAGO, J. H. G.; RAMOS, C. S.; CASANOVA, D. C. C.; MORANDIM, A. A.; BERGAMO, D. C. B.; CAVALHEIRO, A. J.; BOLZANI, V. S.; FURLAN, M.; GUIMARÃES, E. F.; YOUNG, M. C. M.; KATO, M. J. Benzoic acid derivatives from Piper species and their fungitoxic activity against Cladosporium cladosporioides and C. shaerospermum. Journal of Natural Products, Cincinnati, v. 67, n. 11. p. 1783-1788, 2004. DOI: 10.1021/np030530j

LARSEN, M. Biological control of helminths. International Journal for Parasitology, Kidlington, v. 29, n. 1, p. 139-146, 1999. DOI: https://doi.org/10.1016/S0020-7519(98)00185-4

LÓPEZ, M. A.; STASHENKO, E. E.; FUENTES. J. L. Chemical composition and antigenotoxic properties of Lippia alba essential oils. Genetics and Molecular Biology, v.34, n.3, p.479-488, 2011. DOI: https://doi.org/10.1590/S1415-47572011005000030

LORENZI H; MATOS FJA. Plantas medicinais no Brasil: nativas e exóticas. Nova Odessa: Plantarum. 544 p., 2008.

LORENZO, D.; PAZ, D.; DAVIES, P. Composition of a new essential oil type of Lippia alba (Mill.) N. E: Brown from Uruguay. Flavour and Fragrance Journal, v.16, n.5, p.356- 9, 2001. DOI: https://doi.org/10.1002/ffj.1011

MANINI, P. R. Efeito do óleo essencial de piper gaudichaudianum kunth e compostos majoritários em linhagens tumorais [dissertação]. Dissertação (Mestrado) – Programa de Pós-Graduação em Ciências da Saúde [Porto Alegre]: Universidade Federal de

Ciências da Saúde de Porto Alegre; 2015. 85 p. Disponível em: http://repositorio.ufcspa.edu.br/jspui/handle/123456789/165.

MAX, R. A. Effect of repeated wattle tannin drenches on worm burdens, faecal egg counts and egg hatchability during naturally acquired nematode infections in sheep and goats. Veterinary Parasitology, v. 169, p. 138-143, 2010. DOI: https://doi.org/10.1016/j.vetpar.2009.12.022.

MINHO, A. P.; GENNARI, S. M.; ABDALLA, A. L. The effect of condensed tannins on Haemonchus contortus in sheep experimentally infected. Proceedings of the British Society of Animal Science, 2005, p. 139. DOI: http://doi.org/10.1017/S1752756200010504.

MORAIS, S. M., FACUNDO, V. A., BERTINI, L. M., CAVALCANTI, E. S. B., JÚNIOR, J. F. A., FERREIRA, S. A., BRITO, E. S., NETO, M. A. S. Chemical composition and larvicidal activity of essential oils from Piper species. Biochem. Syst. Ecol. 35, 670-675, 2007. DOI: 10.1155/2015/490765

MOREIRA, D. L., SANTOS, P. O., KAPLAN, M. A. C., GUIMARÃES, E. F., CARDOSO, G. L. C., PEREIRA, N. A. Estudos fitoquímicos e farmacológico de Piper gaudichaudianum Kunth (Piperaceae). Rev. Bras. Farm. 82, 29-32, 2001. DOI: https://doi.org/10.5902/1980509831581

OLIVEIRA, T. M. de; ALMEIDA, K. N. S. de; SOUZA, K. B. de; AMARAL, G. C.; CORDEIRO, S. A. Viabilidade econômica de extração de óleo essencial de Corymbia citriodora em Gilbués – PI. Nativa, Sinop, Pesquisas Agrárias e Ambientais. v. 02, n. 03, p. 175-179, jul. /set. 2014. DOI: https://doi.org/10.31413/nativa.v2i3.1612.

OLIVO, C. J.; AGNOLIN, C. A.; PARRA, C. L. C.; VOGEL, F. S. F.; RICHARDS, N. S. P. dos S.; PELLEGRINI, L. G. de; WEBE, A.; PIVOTO, F.; ARAUJO, L. Efeito do óleo de eucalipto (Corymbia citriodora) no controle do carrapato bovino. Ciência rural, v. 43, n. 2, p. 331-337, 2013. DOI: https://doi.org/10.1590/S0103-84782013000200023

OSÓRIO, T. M.; MENEZES, L. de M.; ROSA, K. B. da; ESCOBAR, R. F.; LENCINA, R. M.; MAYDANA, G. M.; SOUZA, V. Q. Resistência anti-helmíntica em nematódeos gastrointestinais na ovinocultura: uma revisão. Brazilian Journal of Development, Curitiba, v. 6, n. 11, p. 89194-89205, 2020. DOI: 10.34117/bjdv6n11-368, 2020.

OSÓRIO, T. M.; COGO, M. R. de M.; MOREIRA, B. P.; SOUZA, V. Q. de. O potencial fitoterápico de espécies do gênero Lippia L. e Aloysia sp.: uma revisão. Revista Eletrônica Científica da UERGS, v. 7, n. 01, p. 19-29, 2021a. DOI: https://doi.org/10.21674/2448-0479.63.13.

OSÓRIO, T. M; MENEZES, L. de M.; ROSA, K. B. da; ESCOBAR, R. F.; SANTOS, R. M.L. dos; MAYDANA, G. de M.; SOUZA, V. Q. de. Seasonal survey of gastrointestinal nematodes in a milk sheep flock. Research, Society and Development, v. 10, n. 3, e34410313315, 2021b. DOI: http://dx.doi.org/10.33448/rsd-v10i3.13315.

PASCUAL, M. E.; SLOWING, K.; CARRETERO, E.; SÁNCHEZ-MATA, D.; VILLAR, A. Lippia: traditional uses, chemistry and pharmacology: a review. Journal of Ethnopharmacology, v. 76, p. 201-214, 2001. DOI: https://doi.org/10.1016/S0378-8741(01)00234-3.

PAULUS, D.; VALMORBIDA, R.; TOFFOLI, E.; NAVA, G. A.; PAULUS, E. Teor e composição química do óleo essencial e crescimento vegetativo de Aloysia triphylla em diferentes espaçamentos e épocas de colheita. Revista Ceres, Viçosa, v. 60, n.3, p. 372-379, 2013. DOI: https://doi.org/10.1590/S0034-737X2013000300010.

PEIXOTO, M. G.; COSTA-JÚNIOR, L. M.; BLANK, A. F.; LIMA, A. da S.; MENEZES, T. S. A.; SANTOS, D. de A.; ALVES, P. B.; CAVALCANTI, S. C. de H.; BACCI, L.; ARRIGONI-BLANK, M. D. F. Acaricidal activity of essential oils from Lippia alba genotypes and its major components carvone, limonene, and citral against Rhipicephalus microplus. Veterinary Parasitology, v. 210, n. 1–2, p. 118-122, 2015. DOI: https://doi.org/10.1016/j.vetpar.2015.03.010.

PÉRES, V. F.; MOURA, D. J.; SPEROTTO, A. R. M. ; DAMASCENO, F. C. ; CARAMÃO, E. B.; ZINI, C. A.; SAFFI, J. Chemical composition and cytotoxic, mutagenic and genotoxic activities of the essential oil from Piper gaudichaudianum Kunth leaves. Food and Chemical Toxicology, v. 47, n. 9, p. 2389–2395, 2009. DOI: https://doi.org/10.1016/j.fct.2009.06.035.

PESSOA, L. M.; MORAIS, S. M.; BEVILAQUA, C. M. L.; LUCIANO, J. H. S. Anthelmintic activity of essential oil of Ocimum gratissimum Linn. and eugenol against Haemonchus contortus. Veterinary Parasitology, Netherlands, v. 109, p. 59-63, 2002. DOI: DOI: 10.1016/S0304-4017(02)00253-4

PIETROBELLI, S. R. EFICIÊNCIA DE PREPARADOS VEGETAIS NO CONTROLE DE DOENÇAS FÚNGICAS E NA INDUÇÃO DE MECANISMOS DE DEFESA EM TOMATEIRO. Dissertação de Mestrado. UNIVERSIDADE FEDERAL DA FRONTEIRA SUL – UFFS. Laranjeiras do Sul, PR. 81p. 2019. Disponível em: https://rd.uffs.edu.br/handle/prefix/2802.

POWERS, K. G.; WOOD, I. B.; ECKERT, J.; GIBSON, T.; SMITH, H. J. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine and ovine). Veterinary Parasitology. 1982 Jul;10(4):265-84. DOI: 10.1016/0304-4017(82)90078-4.

ROBERTS, F. H. S.; O’SULLIVAN, P. J. Methods for egg counts and larval cultures for strongyles infesting the gastro-intestinal tract of cattle. Australian Journal of Agricultural Research 1, 99-102, 1950.DOI: https://doi.org/10.1071/AR9500099

WOOD, I. B.; AMARAL, N.K.; BAIRDEN, K.; DUNCAN, J. L.; KASSAI, T.; MALONE, J. B. JR.; PANKAVICH, J. A.; REINECKE, R. K.; SLOCOMBE, O.; TAYLOR, S. M. et al. World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) second edition of guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine, ovine, caprine). Veterinary Parasitology. 1995 Jun;58(3):181-213. DOI: 10.1016/0304-4017(95)00806-2.

PROCHNOW D.; ALTISSIMO B. S.; SILVA J. C.; MEIRA D.; CARON B. O.; HEINZMANN B. M.; SCHMIDT, D. Chemical composition of the essential oil of Aloysia triphylla (L’Hér) Britton due to water déficit and seasonality. Boletim Latinoamericano y del Caribe de Plantas Medicinais y Aromáticas, 16:121-128, 2017. Disponível em: https://www.researchgate.net/publication/314144686_Chemical_composition_of_the_essential_oil_of_Aloysia_triphylla_L’Her_Britton_due_to_water_deficit_and_seasonality.

RAO, G. P.; SINGH, M.; SINGH, P; SINGH, S. P.; CATALAN, C.; KAPOOR, I. P. S.; SINGH, G. Studies on chemical constituents and antifungal activity of leaf oil of Lippia alba (Mill). Indian Journal of Chemical Technology, v.7, n.6, p.332-5, 2000. Disponível em: http://nopr.niscpr.res.in/handle/123456789/22985.

SANTOS, P. H. D. dos. Produtos alternativos no controle de doenças fúngicas foliares em folha e fruto de mamoeiro. Dissertação de Mestrado. Universidade Estadual do Norte Fluminense Darcy Ribeiro. Rio de Janeiro. 90p. 2013.

SILVA, P. P. da; PÉRES, V. F.; SAFFI, J. Extração e caracterização do óleo essencial das inflorescências de Piper gaudichaudianum Kunth. Revista de Iniciação Científica da Ulbra, v. 1, n. 1, p. 25-30, 2006.

SILVA, D. G., MENEZES, B. M., BETTENCOURT, A. F., FRANTZ, A. C., CORRÊA, M. R., RUSZKOWSKI, G., MARTINS, A. A., BRUM, L. P.; HIRSCHMANN, L. C. Método FAMACHA® como ferramenta para verificar a infestação parasitária ocasionada por Haemonchus spp. em ovinos. PUBVET, 11, 1015-1021, 2017. http://www.pubvet.com.br/artigo/3748/eficaacutecia-anti-helmiacutentica-comparativa-entre-diferentes-princiacutepios-ativos-em-ovinos-jovens

SUTHERLAND, I. A., LEATHWICK, D. M. Anthelmintic resistance in nematode parasites of cattle: a global issue? Trends in Parasitology, n. 27, p.176–181, 2011. DOI: https://doi.org/10.1016/j.pt.2010.11.008.

TOMASINI, P. P. Análise do Óleo Essencial de Piper gaudichaudianum na Citotoxicidade da Doxorrubicina. Dissertação de Mestrado em Patologia da Fundação Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, 252p., 2018.

UENO, H.; GONÇALVES, P. C. Manual para diagnóstico das helmintoses de ruminantes. UFRGS, JICA, Porto Alegre, 143p., 1998.

VON SAMSON-HIMMELSTJERNA, G.; WALSH, T. K.; DONNAN, A. A.; CARRIERE, S.; JACKSON, F.; SKUCE, P. J.; ROHN, K.; WOLSTENHOLME, A. J. Molecular detection of benzimidazole resistance in Haemonchus contortus using real-time PCR and pyrosequencing. Parasitology. 2009;136: 349–358. DOI: http://doi.org/10.1017/S003118200800543X.

WOLFFENBUTTEL, A. N. Base da Química dos Óleos essenciais e Aromaterapia: Abordagem técnica e científica. Belo Horizonte: Ed. Laszlo, 494 p., 2016.

ZEPPENFELD, C. C.; E. M. H. SACCOL, E. M. H.; PÊS, T. S.; SALBEGO, J.; KOAKOSKI, G.l. Aloysia triphylla essential oil as food additive for Rhamdia quelen – Stress and antioxidant parameters. Aquaculture Nutrition, Bergen, v. 23, n. 3, p. 1 - 6, 2017. DOI: https://doi.org/10.1111/anu.12511. Disponível em: https://onlinelibrary.wiley.com/doi/abs/10.1111/anu.12511. Acesso em: 10 nov. 2020.

Downloads

Published

2023-10-20

How to Cite

Osório, T. M., Menezes, L. de M., Martins, A. A., Schimidt, D., Pretto, M. M., Rosa, K. B. da, & Souza, V. Q. de. (2023). Essential oils against gastrointestinal nematodes in sheep in vitro and chemical composition of those plants. Ciência E Natura, 45, e25. https://doi.org/10.5902/2179460X71665

Issue

Vol. 45 (2023): Continuous Publication

Section

Chemistry

License

Copyright (c) 2023 Ciência e Natura

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

To access the DECLARATION AND TRANSFER OF COPYRIGHT AUTHOR’S DECLARATION AND COPYRIGHT LICENSE click here.

 

Ethical Guidelines for Journal Publication

The Ciência e Natura journal is committed to ensuring ethics in publication and quality of articles. 

Conformance to standards of ethical behavior is therefore expected of all parties involved: Authors, Editors, Reviewers, and the Publisher.

In particular, 

Authors: Authors should present an objective discussion of the significance of research work as well as sufficient detail and references to permit others to replicate the experiments. Fraudulent or knowingly inaccurate statements constitute unethical behavior and are unacceptable. Review Articles should also be objective, comprehensive, and accurate accounts of the state of the art. The Authors should ensure that their work is entirely original works, and if the work and/or words of others have been used, this has been appropriately acknowledged. Plagiarism in all its forms constitutes unethical publishing behavior and is unacceptable. Submitting the same manuscript to more than one journal concurrently constitutes unethical publishing behavior and is unacceptable. Authors should not submit articles describing essentially the same research to more than one journal. The corresponding Author should ensure that there is a full consensus of all Co-authors in approving the final version of the paper and its submission for publication.

Editors: Editors should evaluate manuscripts exclusively on the basis of their academic merit. An Editor must not use unpublished information in the editor's own research without the express written consent of the Author. Editors should take reasonable responsive measures when ethical complaints have been presented concerning a submitted manuscript or published paper.

Reviewers: Any manuscripts received for review must be treated as confidential documents. Privileged information or ideas obtained through peer review must be kept confidential and not used for personal advantage. Reviewers should be conducted objectively, and observations should be formulated clearly with supporting arguments, so that Authors can use them for improving the paper. Any selected Reviewer who feels unqualified to review the research reported in a manuscript or knows that its prompt review will be impossible should notify the Editor and excuse himself from the review process. Reviewers should not consider manuscripts in which they have conflicts of interest resulting from competitive, collaborative, or other relationships or connections with any of the authors, companies, or institutions connected to the papers.

Most read articles by the same author(s)