Apomictic seed formation and viability in Ilex paraguariensis A.St.-Hil. (Aquifoliaceae)
DOI:
https://doi.org/10.5902/2179460X84934Keywords:
Apomixis, Dioecious species, Tetrazolium test, Viable seeds, Yerba mateAbstract
The objective of this study was to investigate fruit and seed formation in Ilex paraguariensis A.St.-Hil. without sexual reproduction in addition to evaluating their viability. This study was based on the hypothesis that the formation of apomictic fruit and seeds occurs, but the seeds are non-viable, contributing to the low germination percentage of the species. Five branches of 20 pistillate mother plants were bagged before anthesis (T1) and five immediately after fruit formation (TC). Once they reach maturity, the fruit were collected and counted. Their seeds were cut lengthwise and classified as intact (with embryo and endosperm) or non-intact (empty and/or deteriorated). The viability of intact seeds was evaluated with the tetrazolium test when they were classified as viable or non-viable. The data were submitted to the Kruskal-Wallis Test. The formation of viable fruit and seeds occurred in smaller numbers in T1, all statistically different from TC (p<0.05). Although in smaller quantities and with a statistical difference, the formation of viable fruit and seeds occurs without sexual reproduction, suggesting that the process responsible for their formation is the facultative apomixis. The higher percentage of viable fruit and seeds in TC is probably a result of the occurrence of both apomixis and sexual reproduction. The percentage of non-viable seeds does not differ between the two treatments, suggesting that apomixis may not be the cause of the low percentage of viable seeds and germination, requiring further studies to understand its causes.
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