In vitro germination and seedling formation of Plantago tomentosa Lam. (Plantaginaceae): influence of concentrations of the MS medium
DOI:
https://doi.org/10.5902/2179460X71895Keywords:
Bioactive properties, Folk medicine, Seed propagationAbstract
Plantago tomentosa Lam. (Plantaginaceae) is an herbaceous plant native to Brazil. It is widely used in folk medicine. The species has potential for use in the pharmaceutical and food industry due to its possible bioactive properties and the presence of mucilage in the seeds. The objective of this work was to investigate the influence of different concentrations of Murashige-Skoog (MS) medium on germination and seedling formation in vitro of P. tomentosa. The seeds were collected in Lajeado, disinfected, and inoculated in bottles with MS medium added to 7 g L-1 of agar and 30 g L-1 of sucrose, in four treatments: 25%, 50%, 75%, and 100% of the medium concentration. The experimental design was completely randomized, consisting of 17 replications of ten seeds for each treatment. The evaluation of germination and formed seeds was carried out every two days to determine the variables of germination percentage (GP), mean germination time (MGT), germination speed index (GSI), seedling formation percentage (SFP), mean seedling formation time (MSFT) and seedling formation speed index (SFSI). No significant difference was found between treatments for all the assessed variables. It is concluded that the use of a concentration of 25% of the MS medium is viable, as it promoted a high percentage of germination and seedling formation and in a time interval similar to that of the other concentrations, with the advantage of using fewer reagents.
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Albuquerque, M. M. S., Brito, A. L., Lima, A. P. P. S., Alvim, B. F. M., & Santana, J. R. F. (2016). In vitro establishment of Comanthera curralensis, “sempre viva” native of Chapada Diamantina – Bahia. Ciência Rural, Santa Maria, 46(6), 991-995. DOI: https://doi.org/10.1590/0103-8478cr20131091
Amato-Lourenço, L. S., Rainieri, G. R., Souza, V. C. O., Barbosa Junior, F., Saldiva, P. H. N., & Mauad, T. (2020). Edible weeds: Are urban environments fit for foraging? Science of the Total Environment, Amsterdam, 698. DOI: https://doi.org/10.1016/j.scitotenv.2019.133967
Bareke T. (2018). Biology of seed development and germination physiology. Advances in Plants and Agricultural Research, Edmond, 8(4), 336‒346. DOI: https://doi.org/10.15406/apar.2018.08.00335
Baskin, C. C., & Baskin, J. M. (2014). Seeds: Echology, Biogeography, and Evolution of Dormancy and Germination. 2 ed. Cambridge: Elsevier Academic Press.
Bewley, J. D., Bradford, K. J., Hilhorst, H. W. M., & Nonogaki, H.(2013). Seeds: Physiology of Development, Germination and Dormancy. 3 ed. Berlim: Springer, 2013. DOI: https://doi.org/10.1007/978-1-4614-4693-4
Bolson, M., Hefler, S. R., Chaves, E. I. D., Gasparotto Junior, A., & Cardozo Junior, E. L. (2015). Ethno-medicinal study of plants used for treatment of human ailments, with residents of the surrounding region of forest fragments of Paraná, Brazil. Journal of Ethnopharmacology, Amsterdam, 161, 1-10. DOI: https://doi.org/10.1016/j.jep.2014.11.045
Braga, K. Q., Coimbra, M. C., & Castro, A. H. F. (2015). In vitro germination, callus induction and phenolic compounds contents from Pyrostegia venusta (Ker Gawl.) Miers. Acta Scientiarum. Biological Sciences, Maringá, 37(2), 151-158. DOI: https://doi.org/10.4025/actascibiolsci.v37i2.26067
Cowley, J. M., & Burton, R. A. (2021). The goo-d stuff: Plantago as a myxospermous model with modern utility. New Phytologist, Hoboken, 229, 1917-1923. DOI: https://doi.org/10.1111/nph.17095
Davies Jr., F. T., Geneve, R. L., Wilson, S. B. (2018). Hartmann & Kester’s Plant propagation: principles and practices. 9a ed. USA: Pearson Education.
Deng, W., Hallett, P. D., Jeng, D. S., Squire G. R., Toorop, P. E., & Iannetta, P. P. (2014). The effect of natural seed coatings of Capsella bursapastoris L. Medik. (shepherd’s purse) on soil-water retention, stability and hydraulic conductivity. Plant Soil, Berlim, 387 (1-2), 167-176. DOI: https://doi.org/10.1007/s11104-014-2281-8
Dousseau, S., Alvarenga, A. A., Arantes, L. O., Oliveira, D. M., & Nery, F. C. (2008). Germinação de sementes de tanchagem (Plantago tomentosa Lam.): influência da temperatura, luz e substrato. Ciência e Agrotecnologia, Lavras, 32(2), 438-443. DOI: https://doi.org/10.1590/S1413-70542008000200014
Duarte, M.G.R., Aparecida, I., Soares, A., Brandão, M., Lisieux, R., Jácome, P., & Silva, C.R.F. (2002). Perfil fitoquímico e atividade antibacteriana in vitro de plantas invasoras. Revista Lecta, Bragança Paulista, 20(2), 177-182.
Gadheri-Far, F., Alimagham, S. M., Kameli, A. M., & Jamali, M. (2012). Isabgol (Plantago ovata Forsk) seed germination and emergence as affected by environmental factors and planting depth. International Journal of Plant Production, Gorgan, 6(2).
Grattapaglia, D., & Machado, M. A. (1998). Micropropagação. In: Torres, A. C., Caldas, L. S., Buso, J. A. (Ed.). Cultura de tecidos e transformação de genética de plantas. Brasília, DF: EMBRAPA-SPI: EMBRAPA-CNPH, 1, 183-260.
Hassemer, G.(2020). Plantago tomentosa (Plantaginaceae), not P. virginica naturalised in South Africa: First records of this species outside South America. South African Journal of Botany, Amsterdam, 131, 56-63. DOI: https://doi.org/10.1016/j.sajb.2020.02.012
Hefler, S. M., Rodrigues, W. A.,& Cervi, A. C. (2011). O gênero Plantago (Plantaginaceae) na região sul do Brasil. Revista Brasileira de Biociências, Porto Alegre, 9(3), 297-321.
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