Chemical constituents and larvicidal activity of the microencapsulated essential oil of Citrus aurantium L. against Aedes aegypti

Authors

DOI:

https://doi.org/10.5902/2179460X73726

Keywords:

Bark, Phenolics, larvicidal

Abstract

This study aimed to evaluate the chemical profile and larvicidal activity of the microencapsulated essential oil (EO) of Citrus aurantium L. against Aedes aegypti. The barks of C. aurantium were collected in São Luís-MA. The EO was extracted by hydrodistillation at 100°C for 3h, with chemical characterization through Gas Chromatography coupled with mass spectrometry (GC-MS). Encapsulation of the EO was performed by ionic gelling. To quantify the total phenolic content of the EO, the Folin-Ciocalteu spectrophotometric method was applied. Then, the lethality of the EO against Aedes aegypti was evaluated, and the LC50 for the action of the EO was calculated using the Probit method. The major constituents found in the EO of C. aurantium were: (-)-Terpinen-4-ol (32, 56%) and Caryophyllene oxide (23.52%). The larvicidal activity of the EO showed a LC50 of 3.589 mg L-1. The results indicate that the evaluated EO is composed of substances that provide a good larvicidal effect, revealing its efficiency in combating and controlling Aedes aegypti.

Downloads

Download data is not yet available.

Author Biographies

Breno Pereira Soares, Universidade Federal do Maranhão

Graduated in chemistry on the Universidade Federal do Maranhão (UFMA), researcher at the Laboratory of Research and Application of Essential Oils (LOEPAV/UFMA).
Research area: natural products

Thaylanna Pinto de Lima, Universidade Federal do Maranhão

Graduating in industrial chemistry on the Universidade Federal do Maranhão (UFMA), researcher at the Laboratory of Research and Application of Essential Oils (LOEPAV/UFMA).
Research area: natural products

Ana Paula Muniz Serejo, Universidade Federal do Maranhão

Biotechnology PhD student (REBORNIO), professor (Faculdade Maurício de Nassau), researcher at the Laboratory of Research and Application of Essential Oils (LOEPAV/UFMA).
Research area: natural products

Rodrigo Aquino de Almeida, Universidade Federal do Maranhão

Graduating in chemistry on the Universidade Federal do Maranhão (UFMA), researcher at the Laboratory of Research and Application of Essential Oils (LOEPAV/UFMA).
Research area: natural products

Brendha Araújo de Sousa, Universidade Federal do Maranhão

Graduated in chemistry on the Universidade Federal do Maranhão (UFMA), researcher at the Laboratory of Research and Application of Essential Oils (LOEPAV/UFMA).
Research area: natural products

Beatriz Jardim Rodrigues das Chagas, Universidade Federal do Maranhão

Graduating in chemistry on the Universidade Federal do Maranhão (UFMA), researcher at the Laboratory of Research and Application of Essential Oils (LOEPAV/UFMA).
Research area: natural products

Marcelle Adriane Ataide Matos, Universidade Federal do Maranhão

Graduating in chemistry on the Universidade Federal do Maranhão (UFMA), researcher at the Laboratory of Research and Application of Essential Oils (LOEPAV/UFMA).
Research area: natural products

 

Victor Elias Mouchrek Filho, Universidade Federal do Maranhão

Professor at the Universidade Federal do Maranhão (UFMA), researcher at the Laboratory of Research and Application of Essential Oils (LOEPAV/UFMA).
Research area: natural products

Gustavo Oliveira Everton, Universidade Federal do Maranhão

Chemistry PhD student (Universidade Federal do Maranhão), researcher at the Laboratory of Research and Application of Essential Oils (LOEPAV/UFMA).
Research area: natural products

References

Adams, R. P. (2017) Identification of essential oil components by gas chromatography/mass spectrometry. 5.ed. TX (USA): Texensis Publishing.

Ambriz-Pérez, D. L. et al. (2016). Phenolic compounds: Natural alternative in inflammation treatment. A Review. Cogent Food & Agriculture, 2(1), 1131412. doi: 10.1080/23311932.2015.1131412. DOI: https://doi.org/10.1080/23311932.2015.1131412

Anagnostopoulou, M. A. et al. (2006). Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food chemistry, 94(1), 19-25. doi: 10.1016/j.foodchem.2004.09.047. DOI: https://doi.org/10.1016/j.foodchem.2004.09.047

Assunção, G. V. (2013). Chemical characterization and evaluation of the larvicidal activity front Aedes aegypti of the essential oil of the Citrus sinensis l. osbeck (sweet orange) species. [Dissertação de Mestrado em Química, Universidade Federal do Maranhão]. Repositório Institucional da UFMA.

Benelli, P. (2010). Agregação de valor ao bagaço de laranja (Citrus sinensis L. Osbeck) mediante obtenção de extratos bioativos através de diferentes técnicas de extração. [Dissertação de Mestrado em Engenharia de Alimentos, Universidade Federal do Maranhão] Repositório Institucional da UFMA.

Cheng, S. S. et al. (2003). Bioactivity of selected plant essential oils against the yellow fever mosquito Aedes aegypti larvae. Bioresource Technology, 89(1), 99-102. doi: 10.1016/S0960-8524(03)00008-7. DOI: https://doi.org/10.1016/S0960-8524(03)00008-7

Choudhury, N., Meghwal, M., & Das, K. (2021). Microencapsulation: An overview on concepts, methods, properties and applications in foods. Food Frontiers, 2(4), 426-442. DOI: https://doi.org/10.1002/fft2.94

Costa, M. J. R. (2015). Determinação da atividade antioxidante e compostos fenólicos totais em óleos essenciais. [Dissertação de Graduação do Curso Tecnólogo em Processos Químicos), Universidade Tecnológica Federal do Paraná]. Repositório Institucional da UTFPR.

Dubey, R. et al. (2009). Microencapsulation technology and applications. Defence science journal, 59(1), p. 82-95. https://core.ac.uk/download/pdf/333719984.pdf.

Fidélix, M., Milenkovic, D., Sivieri, K., & Cesar, T. (2020). Microbiota modulation and effects on metabolic biomarkers by orange juice: A controlled clinical trial. Food & function, 11(2), 1599-1610. DOI: https://doi.org/10.1039/C9FO02623A

Finney, D. J. (1952). The estimation of the ED50 for a logistic response curve. Sankhyā: The Indian Journal of Statistics,12(1/2),121-136. https://www.jstor.org/stable/25048121.

Forattini, O, P. (1962). Entomologia médica. Revista Saúde Pública, 41(6), 185-302. https://www.scielosp.org/pdf/rsp/v41n6/03.pdf.

Goneli, A. (2008). Variation of physical-mechanical properties and quality of castor bean (Ricinus communis L.) during drying and storage. [Tese de Doutorado em Doutorado em Construções rurais e ambiência; Energia na agricultura; Mecanização agrícola; Processamento de produção, Universidade Federal de Viçosa]. Repositório Institucional da UFV.

Irshad, M., Subhani, M. A., Ali, S., & Hussain, A. (2020). Biological importance of essential oils. Essential Oils-Oils of Nature, 1, 37-40. DOI: https://doi.org/10.5772/intechopen.87198

Jain, P. L., Patel, S. R., & Desai, M. A. (2022). Patchouli oil: An overview on extraction method, composition and biological activities. Journal of Essential Oil Research, 34(1), 1-11. DOI: https://doi.org/10.1080/10412905.2021.1955761

Jiang, T., Liao, W., & Charcosset, C. (2020). Recent advances in encapsulation of curcumin in nanoemulsions: A review of encapsulation technologies, bioaccessibility and applications. Food Research International, 132, 109035. DOI: https://doi.org/10.1016/j.foodres.2020.109035

Ju, J., Xie, Y., Guo, Y., Cheng, Y., Qian, H., & Yao, W. (2020). Application of starch microcapsules containing essential oil in food preservation. Critical reviews in food science and nutrition, 60(17), 2825-2836. DOI: https://doi.org/10.1080/10408398.2018.1503590

Khalid, K. A., Darwesh, O. M., & Ahmed, A. M. (2021). Peel essential oils of citrus types and their antimicrobial activities in response to various growth locations. Journal of Essential Oil Bearing Plants, 24(3), 480-499. DOI: https://doi.org/10.1080/0972060X.2021.1941278

Leite, A. M. et al. (2009). Preliminary study of the molluscicidal and larvicidal properties of some essential oils and phytochemicals from medicinal plants. Revista Brasileira de Farmacognosia, 19(4), 842-846. doi: 10.1590/S0102-695X2009000600008. DOI: https://doi.org/10.1590/S0102-695X2009000600008

Leite, D. D. F. et al. (2017). Mathematical modeling of pineapple peel drying kinetics. Green Journal of Agroecology and Sustainable Development. Revista Verde de Agroecologia e Desenvolvimento Sustentável, 12(4), 769-774. doi: 10.18378/rvads.v12i4.4980. DOI: https://doi.org/10.18378/rvads.v12i4.4980

Martins, G. S. O et al. (2017). Chemical composition and toxicity of of citrus essential oils on Dysmicoccus brevipes (hemiptera: pseudococcidae). Revista Caatinga, 30(3), 811- 817. https://www.scielo.br/j/rcaat/a/ss8NHJJCZpncgvbdryKqxHf/. doi: 10.1590/1983-21252017v30n330rc. DOI: https://doi.org/10.1590/1983-21252017v30n330rc

Nascimento, A. A. et al. (2016) Atividade larvicida do óleo essencial de cravo-da-índia: Extração, caracterização e atividade larvicida frente ao mosquito Aedes aegypti. 1. ed. International Book Market Service, Riga(Letônia): Editora Novas Edições Acadêmicas.

Oliveira, M. B. (2013). Extraction, characterization and evaluation of larvicidal activity essential oil of linnaeus citrus limon (lemon) front mosquito Aedes aegypti. [Dissertação de Mestrado em Química, Universidade Federal do Maranhão]. Repositório Institucional da UFMA.

Sales, E. H. et al. (2020). Drying, toxicity and antimicrobial potential of Curcuma longa L essential oil. Research, Society and Development, 9(8), p. e511985600. doi: 10.33448/rsd-v9i8.5600. DOI: https://doi.org/10.33448/rsd-v9i8.5600

Santos, A. B. S. et al. (2020). Óleos essenciais de Cinnamomum zeylanicum Blume e Plectranthus amboinicus (lour) Spreng como agentes larvicidas frente as larvas do Aedes aegypti. Brazilian Journal of Development, 6(4), p. 22355-22369. doi: 10.33448/rsd-v9i8.5600. DOI: https://doi.org/10.34117/bjdv6n4-413

Silva, H. H. G. D, et al. (1995). Physiological age of aedes stegomyia aegypti eggs. Linnaeus, (1762) diptera, culicidae. Revista de Patologia Tropical, 24(2), 269- 273. doi: 10.5216/rpt.v24i2.19696. DOI: https://doi.org/10.5216/rpt.v24i2.19696

Silva, W. J. (2006). Larvicidal activity of essential oil from plants existing in the state of Sergipe against Aedes aegypti Linn. [Dissertação de Mestrado em Desenvolvimento e Ambiente, Universidade Federal de Sergipe. Repositório Institucional da UFS.

Silvestre, J. D. F. et al. (2010). Perfil da composição química e Atividade antibacteriana e antioxidante do óleo essencial do cravo-da-índia (Eugenia caryophyllata Thunb.). Revista Ceres, 57(5), 589-594. https://www.scielo.br/j/rceres/a/tgjGTK5Lj95w5HdfHMPnN9N/. doi: 10.1590/S0034-737X2010000500004. DOI: https://doi.org/10.1590/S0034-737X2010000500004

Simões, C. M. et al. (2007) Pharmacognosy: from plant to medicine. Porto Alegre (Brasil): Editora UFRGS.

Singh, H. P. et al. (2012). Assessment of in vitro antioxidant activity of essential oil of Eucalyptus citriodora (lemon-scented Eucalypt; Myrtaceae) and its major constituents. LWT-Food science and Technology, 48(2), 237-241. doi: 10.1016/j.lwt.2012.03.019. DOI: https://doi.org/10.1016/j.lwt.2012.03.019

Sousa, D. A. (2019). Avaliação da atividade larvicida de óleos essenciais de espécies de citrus frente às larvas de Aedes aegypti. 2019. [Dissertação de Mestrado em Saúde e Ambiente, Universidade Federal do Maranhão]. Repositório Institucional da UFMA.

Waterhouse, A. L. (2002). Determination of total phenolics. Current protocols in food analytical chemistry, 6(1), 1-8. DOI: https://doi.org/10.1002/0471142913.fai0101s06

Downloads

Published

2024-09-06

How to Cite

Soares, B. P., Lima, T. P. de, Serejo, A. P. M., Almeida, R. A. de, Sousa, B. A. de, Chagas, B. J. R. das, Matos, M. A. A., Mouchrek Filho, V. E., & Everton, G. O. (2024). Chemical constituents and larvicidal activity of the microencapsulated essential oil of Citrus aurantium L. against Aedes aegypti. Ciência E Natura, 46, e73726. https://doi.org/10.5902/2179460X73726

Most read articles by the same author(s)

<< < 1 2 

Similar Articles

You may also start an advanced similarity search for this article.