Determination of pesticides in hydroponic water for environmental phytoremediation

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DOI:

https://doi.org/10.5902/2179460X68460

Keywords:

HPLC-DAD, SPE, Lactuca sativa

Abstract

This study validated a simple, and fast method by High Performance Liquid Chromatography with Diode Array Detector (HPLC -DAD) for pesticide phytoremediation analysis. The method was developed in water and in a hydroponic medium. Sample extraction and concentration were performed by Solid Phase Extraction (SPE) with Strata C18-E type cartridges. The SPE-HPLC-DAD method was successfully applied in the detection and quantification of quinclorac, 2,4-D, propanil, bentazon, clomazone and tebuconazole in water and hydroponic medium for 14 days. The method presented excellent results with the linearity of 0.9969 - 0.9994 and the lowest limit of detection (LOD) and quantification (LOQ) of pesticides was 1.7 μg/L and 5.0 μg/L, respectively with RSD <11.92%. The average recovery obtained ranged from 77.62% to 109.73% and RSD <12.70%. A Lactuca sativa species promoted phytoremediation on the 7th day for 2,4-D and tebuconazole and on the 14th day for clomazone.

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Author Biographies

Anderson da Silva Rosa, Universidade Federal do Pampa, Uruguaiana, RS, Brasil

Graduado em Química - Licenciatura Plena pela Universidade Federal de Santa Maria (UFSM), possui especialização em Gestão Ambiental e Desenvolvimento Sustentável (UNINTER) e Mestrado em Bioquímica pela Universidade Federal do Pampa (Unipampa). Possui experiência em síntese orgânica, análise de solos, água e tecido vegetal, análise de pesticidas e fitorremediação. Atualmente é químico da Universidade Federal do Pampa - UNIPAMPA e doutorando do PPG Educação em Ciências: Química da Vida e Saúde atuando em pesquisas em ensino de ciências com ênfase em mobile learning aplicada ao ensino de química.

Bruna Piaia Ramborger, Universidade Federal do Pampa, Uruguaiana, RS, Brasil

Possui graduação em Farmácia pela Universidade Federal do Pampa - Campus Uruguaiana-RS (UNIPAMPA) (2015), Mestrado e Doutorado em Bioquímica pelo programa de Pós-Graduação em Bioquímica da UNIPAMPA (2015-2022). Faz parte do grupo de pesquisa do Laboratório de Análises Químicas Ambientais e Toxicológicas - LAQAT, vinculado a este programa (PPG bioquímica UNIPAMPA). Possui experiência na área de fitorremediação de contaminantes, preparo de amostras, uso de modelos experimentais alternativos (Allium cepa, Artemia salina e Drosophila melanogaster), e análises cromatográficas, principalmente em cromatografia líquida de alta eficiência.

Maria Luisa Candido Zago, Universidade Federal do Pampa, Uruguaiana, RS, Brasil

Residente em Farmácia do Programa de Residência Integrada Multiprofissional em Urgência e Emergência, UNIPAMPA. Farmacêutica graduada pela Universidade Federal do Pampa- Campus Uruguaiana. Realizou atividades associadas ao Laboratório de Estudos Físico- Químicos e Produtos Naturais - LEFQPN,e ao Grupo Interdisciplinar de Pesquisa em Práticas de Ensino(GIPPE), vinculada ao programa de pós-graduação em Bioquímica da Unipampa.

Dandara Fidélis Escoto, Universidade Federal do Pampa, Uruguaiana, RS, Brasil

Cientista da Natureza Licenciada pela Universidade Federal do Pampa - Campus Uruguaiana (2014), Mestra em Bioquímica pelo Programa de Pós-graduação em Bioquímica da Unipampa - Campus Uruguaiana (2017). Fez parte do Grupo Interdisciplinar de Pesquisa em Práticas de Ensino (GIPPE) como pesquisadora nas áreas de Bioquímica de produtos biologicamente ativos e ensino de ciências desde 2010, possui experiência na área de ensino e formação de educadores para o ensino básico e superior. É professora na rede municipal de Uruguaiana atuando na área de ciências e ensino religioso nos anos finais na EMEF Moacyr Ramos Martins.

Elton Luis Gasparotto Denardin, Universidade Federal do Pampa, Uruguaiana, RS, Brasil

Possui graduação em Bacharelado e Licenciatura em Química pela Universidade Federal do Paraná (1996), mestrado em Química pela Universidade Federal do Rio Grande do Sul (2000) e doutorado em Química pela Universidade Federal do Rio Grande do Sul (2004). Atualmente, é Professor Associado IV (DE) da Universidade Federal do Pampa (UNIPAMPA)/Campus Uruguaiana e docente permanente do Programa de Pós-Graduação em Bioquímica (2011) e do Programa de Pós-Graduação em Ciências Farmacêuticas (2021). Tem experiência na área de Química, com ênfase em Físico-Química, Produtos Naturais e nanomateriais atuando principalmente nos seguintes temas: polímeros naturais e sintéticos, técnicas espectroscópicas, cromatográficas e de análise térmica, produtos naturais, nanomateriais e doenças neurodegenativas.

Rafael Roehrs, Universidade Federal do Pampa, Uruguaiana, RS, Brasil

Possui graduação em Química Industrial pela Universidade de Santa Cruz do Sul (2000), mestrado em Biologia Celular e Molecular pela Universidade Federal do Rio Grande do Sul (2004) e doutorado em Química Analítica pela Universidade Federal de Santa Maria (2009). Atualmente é docente no curso de Ciências da Natureza - Licenciatura, no Programa de Pós-Graduação em Bioquímica e no Programa de Pós-Graduação em Educação em Ciências: Química da Vida e Saúde da Universidade Federal do Pampa. Sou Líder do Grupo Interdisciplinar de Pesquisa em Prática de Ensino (GIPPE) e do Laboratório de Análises Químicas Ambientais e Toxicológicas (LAQAT), e é pesquisador do Laboratório de Estudos Físico-Químicos e Produtos Naturais (LEFQPN). Na área de Química Analítica, orienta principalmente nas áreas de fitorremediação, análise por cromatografia líquida de neurotransmissores, pesticidas e seus metabólitos, compostos do metabolismo secundário das plantas. Na área de Ensino de Ciências, orienta sobre games e gamificação, planejamento colaborativo e avaliação no ensino de Ciências.

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2022-06-13 — Updated on 2022-07-06

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Rosa, A. da S., Ramborger, B. P., Zago, M. L. C., Escoto, D. F., Denardin, E. L. G., & Roehrs, R. (2022). Determination of pesticides in hydroponic water for environmental phytoremediation. Ciência E Natura, 44, e27. https://doi.org/10.5902/2179460X68460 (Original work published June 13, 2022)

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Vol. 44 (2022): Continuous Publication

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Environment

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