Evaluation of the cytotoxic and genotoxic potentials of the solubilized extract of the coffee waste using Allium cepa as a test system

Authors

DOI:

https://doi.org/10.5902/2179460X34552

Keywords:

Cytotoxicity, Genotoxicity, Coffee waste

Abstract

The residual biomasses of coffee and other beverages generated in coffee shops, restaurants, residences, bars among others do not have registers due to the difficulty of quantifying this type of waste in different places. To analyze the toxic effects for substance or mixed substances improperly discarded should be performed toxicity tests that aim to predict the potential impact of a xenobiotic on the environment. The objective of this work was to evaluate the impact of the solubilized extract of coffe waste on the germination and development of onion seedlings (A. cepa), as well as to evaluate the cytotoxic and genotoxic potential of this extract in onion root meristematic cells. The experimental design was completely randomized, consisting of five treatments for the test group and two control group. For all treatments, 5 replicates were used. To evaluate the cytotoxicity and genotoxicity test, 5 seedlings of each treatment were removed after the germination process. Thus, from the treatment T2, the extract was able to provide a deleterious effect on germination and root growth. The extract presented a genotoxic effect in the concentration of T2 to T5 treatments, and in addition to genotoxicity, T3 to T5 treatments also showed a cytotoxic effect.

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Author Biographies

Priscyanne Barreto Siqueira, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ

Graduação em Ciências Biológicas - Bacharelado pela Universidade Federal Rural do Rio de Janeiro

Viviane Moreira de Lima, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ

Professora do Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro– UFRRJ.

Thuanny Moares de Almeida, Universidade Federal do Rio de Janeiro

Doutoranda em Ciência e Tecnologia de Polímeros IMA/UFRJ. Colaboradora do Laboratório Multidisciplinar de Gerenciamento de Resíduos (LMGR).

Heider Alves Franco, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Pinheiral, RJ

Professor do Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro – IFRJ, campus Pinheiral, RJ. Laboratório Multidisciplinar de Tecnologia Agroambiental (LAMTAA)

Hélcio Resende Borba, Universidade Federal Rural do Rio de Janeiro, Seropédica, RJ

Professor(a) do Departamento de Biologia Animal, Instituto de Ciências Biológicas e da Saúde, Universidade Federal Rural do Rio de Janeiro– UFRRJ.

Sérgio Thode Filho, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Duque de Caxias, RJ

Professor do Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro - IFRJ, campus Duque de Caxias, RJ. Laboratório Multidisciplinar de Gerenciamento de Resíduos (LMGR).

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Published

2018-03-27

How to Cite

Siqueira, P. B., Lima, V. M. de, Almeida, T. M. de, Franco, H. A., Borba, H. R., & Thode Filho, S. (2018). Evaluation of the cytotoxic and genotoxic potentials of the solubilized extract of the coffee waste using Allium cepa as a test system. Ciência E Natura, 40, e77. https://doi.org/10.5902/2179460X34552

Issue

CIÊNCIA E NATURA, V. 40, 2018

Section

Environment

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