Evaluation of intestinal histological damage in zebrafish exposed to environmentally relevant concentrations of manganese

Authors

DOI:

https://doi.org/10.5902/2179460X31681

Keywords:

Danio rerio, Histopathology, Intestine, Alcian Blue

Abstract

Manganese is an essential element for many physiological processes. However, in prolonged exposures or at high concentrations it may present toxicity to several organs, which has made it an object of study in pharmacological, behavioral, neurological and environmental research in different organisms. Thus, it was aimed to evaluate the damage caused by acute and chronic exposures to this element, through the histopathological analysis of the zebrafish (Danio rerio) intestine. Samples were embedded in paraffin for further processing in rotating microtome, and the slides were stained with Alcian Blue and hematoxylin for observation and analysis under light microscopy. The results demonstrate the ability of the metal to cause histological changes in this organ and to increase the secretion of mucus from globet cells in the region analyzed, mainly in the highest concentrations of Manganese Chloride. It is important to monitor the concentrations of this and other metals in the water bodies, given their toxicity.

Downloads

Download data is not yet available.

Author Biographies

Gabriela Zimmermann Prado Rodrigues, Universidade Feevale, Novo Hamburgo, RS

Doutorado em andamento em Qualidade Ambiental, Universidade Feevale

Mateus Santos de Souza, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS

Mestrando do PPG em Biologia Animal da Universidade Federal do Rio Grande do Sul

Antonia Homrich da Silva, Universidade Feevale, Novo Hamburgo, RS

Graduanda na Universidade Feevale, bolsista Junior CNPq do Laboratório de Histologia Comparada

Bruna Graziela Zwetsch, Universidade Feevale, Novo Hamburgo, RS

Graduada Ciências Biológicas pela Universdiade Feevale

Günther Gehlen, Universidade Feevale, Novo Hamburgo, RS

Professor adjunto da Universidade Feevale e membro do PPG-Qualidade ambiental da Universidade Feevale

References

AUGUSTINE S, PEREIRA S, FLORIANI M, CAMILLERI V, KOOJIMAN SALM, GAGNAIRE B, ADAM-GUILLERMIN C. Effects of chronic exposure to environmentally relevant concentrations of waterborne depleted uranium on the digestive tract of zebrafish, Danio rerio. J. Environ. Radioact. 2015; 142: 45-53.

BRASIL. Conselho Nacional do Meio Ambiente – CONAMA, 2005 [viewed 25 November 2014]. Resolução CONAMA nº 357, de 17 de março de 2005. Dispõe sobre a classificação dos corpos de água e diretrizes ambientais para o seu enquadramento, bem como estabelece as condições e padrões de efluentes, e dá outras providencias. Diário Ofi cial da República Federativa do Brasil [online], Brasilia, 18 mar. pp. 58-63. Available from: http://www.mma.gov.br/port/conama/res/res05/res35705.pdf

BRUGMAN S, WITTE M, SCHOLMAN RC, KLEIN MR, BOES M, NIEUWENHUIS EE. T lymphocyte-dependent and -independent regulation of Cxcl8 expression in zebrafish intestines J. Immunol. 2014;192: 484-491.

CHEN L. et al., Intestinal immune function, antioxidant status and tight junction proteins mRNA expression in young grass carp (Ctenopharyngodon idella) fed riboflavin deficient diet. Fish Shellfish Immunol. 2015;47: 470-84.

DA SILVA WF, SIMÕES MJ, GUTIERRE RC, EGAMI MI, SANTOS AA, ANTONIAZZI MM, SASSO GR, RANZANI-PAIVA MJT. Special dyeing, histochemistry, immunohistochemistry and ultrastructure: A study of mast cells/eosinophilic granules cells (MCs/EGC) from Centropomus parallelus intestine. Fish Shellfish Immunol. 2017;60: 502-8.

DALZOCHIO T. et al. Water Quality Parameters, Biomarkers and Metal Bioaccumulation in Native Fish Captured in the Ilha River, Southern Brazil. Chemosphere. 2017; 189;609-18.

DOS SANTOS HB, VIEIRA L, ALVES SN, THOMÉ RG. Análise do intestino delgado de Danio rerio exposto a organofosforado e detergente: um estudo histológico e morfométrico. Revista Conexão Ciência. 2016;11(2); 51-58.

ERIKSON KM, ASCHNER M. Manganese neurotoxicity and glutamate-GABA interaction. Neurochem Int. 2003;43(4-5): 475-80.

FLEMING A, JANKOWSKI J, GOLDSMITH P. In vivo analysis of gut function and disease changes in a zebrafish larvae model of inflammatory bowel disease: a feasibility study. Inflamm. Bowel Dis. 2010;16; 1162-1172.

GOLUB MS, HOGREFE CE, GERMANN SL, TRAN TT, BEARD JL, CRINELLA FM, LONNERDAL B. Neurobehavioral evaluation of rhesus monkey infants fed cow's milk formula, soy formula, or soy formula with added manganese. Neurotoxicol Teratol. 2005;27: 615-27.

HARIKUMAR PS, NASIR UP. Ecotoxicological impact assessment of heavy metals in core sediments of a tropical estuary. Ecotoxicol Environ Saf. 2010;73(7): 1742 –1747.

HERMES N. et al., Environmental pathways and human exposure to manganese in southern Brazil. An. Acad. Bras. Ciênc. 2013;85(4): 1275-88.

ISO; International Organization for Standardization. Water quality - Determination of the acute lethal toxicity of substances to a freshwater fish [Brachydanio rerio Hamilton-Buchanan (Teleostei, Cyprinidae)]. ISO 7346-3: Flow-through method. 1996. Available: [http://www.iso.org]

NAIR M, JAYALAKSHMY KV, BALACHANDRAN KK, JOSEPH T. Bioaccumulation of toxic metals by fish in a semi-enclosed tropical ecosystem. Environ Forensics. 2006;7: 197–206.

OEHLERS SH, FLORES MV, HALL CJ, CROSIER KE, CROSIER PH. Retinoic acid suppresses intestinal mucus production and exacerbates experimental enterocolitis Dis. Models Mech. 2012;5: 457-467.

OLIVEIRA MTG, ROLIM SBA, FARIAS PCM, MENEGUZZI A, LUTCKMEI C. Industrial pollution of environmental compartments in the Sinos River Valley, RS, Brasil: Geochemical- Biogeochemical characterization and remote sensing. Water Air Soil Pollution, 2008;192:183-198.

PATIL DS, CHAVAN SM, OUBAGARANADIN JUK. A review of technologies for manganese removal from wastewaters. J Environ Chem Eng. 2016;4:468- 487.

RIETZLER AC, FONSECA AL, LOPES GP. Heavy metals in tributaries of Pampulha Reservoir, Minas Gerais. Braz. J. Biol. 2001;61(3): 363-370.

ROCHA PMC, BARROS MEG, EVÊNCIO-NETO J. Análise morfométrica da parede intestinal e dinâmica de mucinas secretadas no jejuno de frangos suplementados com probiótico Bacillus subtilis cepa C3102. Pesq. Vet. Bras. 2016; 34(4);312-16.

SANKAR TV, ZYNUDHEEN AA, ANANDAN R, VISWANATHAN NAIR PG. Distribution of organochlorine pesticides and heavy metal residues in fish and shellfish from Calicut region, Kerala, India. Chemosphere. 2006;65: 583–590.

SANTOS DCM, MATTA SLP, DERGAM JÁ, OLIVEIRA JÁ. Alterações histológicas em brânquias de Astyanax aff. bimaculatus causado por exposição aguda ao zinco. Patologia Experimental e Toxicológica, 2015; 64: 861-866.

SFACTERIA A, BRINES M, BLANK U. The mast cell plays a central role in the immune system of teleost fish. Mol Immunol. 2015; 63: 3-8.

SMITH WS, ESPÍNDOLA ELG, ROCHA O. Environmental gradient in reservoirs of the medium and low Tietê River: limnological differences through the habitat sequence. Acta Limnol. Bras.. 2014;26(1): 73-88.

SUBOTIC S, VISNJIÉ-JEFTIC Z, SPASIC S, HEGEDIS A, KRPO-CETKOVIC J, LENHARDT M. Concentrations of 18 Elements in Muscle, Liver, Gills, and Gonads of Sichel (Pelecus cultratus), Ruffe (Gymnocephalus cernua), and European Perch (Perca fluviatilis) in the Danube River near Belgrade (Serbia). Water Air Soil Pollut, 2015;226: 1-11.

SVENSSON-FREJ M. Immunobiology of intestinal eosinophils - a dogma in the changing? J Innate Immun. 2011;3:565–57.

WHO. World Health Organization. 25º Progress on Sanitation and Drinking Water. 2015 Update and MDG Assessment. 2015.

WITTE M, HUITEMA LF, NIEUWENHUIS EE, BRUGMAN S. Deficiency in macrophage-stimulating protein results in spontaneous intestinal inflammation and increased susceptibility toward epithelial damage in zebrafish. Zebrafish, 2014;11: 542-550.

YANG HT, ZOU SS, ZHAI LJ, WANG Y, ZHANG FM, AN LG, YANG GW. Pathogen invasion changes the intestinal microbiota composition and induces innate immune responses in the zebrafish intestine. Fish Shellfish Immunol. 2017;71: 35-42.

ZHAI Q, LI T, YU L, XIAO Y, FENG S, WU J, ZHAO J, ZHANG H, CHEN W. Effects of subchronic oral toxic metal exposure on the intestinal microbiota of mice. Sci. Bull. 2017;12: 831-840.

ZHANG S, ZHOU Z, FU J. Effect of manganese chloride exposure on liver and brain mitochondria function in rats. Environ. Res. 2003;93(2): 147-57.

Downloads

Published

2018-03-27

How to Cite

Prado Rodrigues, G. Z., Souza, M. S. de, Silva, A. H. da, Zwetsch, B. G., & Gehlen, G. (2018). Evaluation of intestinal histological damage in zebrafish exposed to environmentally relevant concentrations of manganese. Ciência E Natura, 40, e52. https://doi.org/10.5902/2179460X31681

Issue

CIÊNCIA E NATURA, V. 40, 2018

Section

Environment

License

To access the DECLARATION AND TRANSFER OF COPYRIGHT AUTHOR’S DECLARATION AND COPYRIGHT LICENSE click here.

 

Ethical Guidelines for Journal Publication

The Ciência e Natura journal is committed to ensuring ethics in publication and quality of articles. 

Conformance to standards of ethical behavior is therefore expected of all parties involved: Authors, Editors, Reviewers, and the Publisher.

In particular, 

Authors: Authors should present an objective discussion of the significance of research work as well as sufficient detail and references to permit others to replicate the experiments. Fraudulent or knowingly inaccurate statements constitute unethical behavior and are unacceptable. Review Articles should also be objective, comprehensive, and accurate accounts of the state of the art. The Authors should ensure that their work is entirely original works, and if the work and/or words of others have been used, this has been appropriately acknowledged. Plagiarism in all its forms constitutes unethical publishing behavior and is unacceptable. Submitting the same manuscript to more than one journal concurrently constitutes unethical publishing behavior and is unacceptable. Authors should not submit articles describing essentially the same research to more than one journal. The corresponding Author should ensure that there is a full consensus of all Co-authors in approving the final version of the paper and its submission for publication.

Editors: Editors should evaluate manuscripts exclusively on the basis of their academic merit. An Editor must not use unpublished information in the editor's own research without the express written consent of the Author. Editors should take reasonable responsive measures when ethical complaints have been presented concerning a submitted manuscript or published paper.

Reviewers: Any manuscripts received for review must be treated as confidential documents. Privileged information or ideas obtained through peer review must be kept confidential and not used for personal advantage. Reviewers should be conducted objectively, and observations should be formulated clearly with supporting arguments, so that Authors can use them for improving the paper. Any selected Reviewer who feels unqualified to review the research reported in a manuscript or knows that its prompt review will be impossible should notify the Editor and excuse himself from the review process. Reviewers should not consider manuscripts in which they have conflicts of interest resulting from competitive, collaborative, or other relationships or connections with any of the authors, companies, or institutions connected to the papers.

Most read articles by the same author(s)