Environmental assessment of luiz Rau Stream (Brazil) utilizing allium cepa test

Gabriela Zimmermann Prado Rodrigues, Aline Belem Machado, Mariana Finkler, Daiane Bolzan Berleze, Günther Gehlen

Abstract


The aim of this study was to monitor the water quality of Luiz Rau stream, through physicochemical parameters, environmental characteristics of the site, and genotoxicity test in Allium cepa. Four water samples from Luiz Rau stream were collected once a week for one month and some physicochemical parameters were verified. The environmental characteristics were performed by the Quick Assessment Protocol (QAP) and evaluated at the sampling site. For the genotoxicity assay, the Allium cepa test was utilized. In relation to the water physicochemical parameters, low levels of dissolved oxygen (DO) and high levels of ammonia, and phosphorus were obtained, which demonstrates the poor quality of the stream. The QAP results identified the stream as impacted. The mitotic index parameter was altered in the seed exposed to raw water samples in the third and fourth sample collection, demonstrating cell cycle reduction and proliferation respectively; witch can be associated with the presence of contaminated effluents in the water. In relation to the genotoxicity, the micronuclei were increased in seeds exposed to four raw samples in comparison to the control group. Therefore, the results obtained can characterize the Luiz Rau stream as impacted, mostly by anthropic actions, corroborating with other publications.


Keywords


Citotoxicity; Genotoxicity; Quick Assessment Protocol; Rainfall

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References


ALBERTINI RJ, ANDERSON D, DOUGLAS GR, HAGMAR L, HEMMINKI K, MERLO F et al. IPCS guidelines for the monitoring of genotoxic effects of carcinogens in humans. Mutat Res. 2000;463:111–172.

BENVENUTTI T, KIELING-RUBIO MA, KLAUCK CR, RODRIGUES MAS. Evaluation of water quality at the source of streams of the Sinos River Basin, Southern Brazil. Braz. J. Biol. 2015;75, Suppl 2:S98–S104

BIANCHI J, MANTOVANI MS, MARIN-MORALES MA. Analysis of the genotoxic potential of low concentrations of Malathion on the Allium cepa cells and rat hepatoma tissue culture. J. Environ. Sci. 2015;36:102–111.

BIANCHI E, LESSING G, BRINA KR, ANGELI L, ANDRIGUETTI NB, PERUZZO JRS et al. Monitoring the Genotoxic and Cytotoxic Potential and the Presence of Pesticides and Hydrocarbons in Water of the Sinos River Basin, Southern Brazil. Arch Environ Contam Toxicol. 2017;72:321–334.

CALLISTO M, FERREIRA WR, MORENO P, GOULART M, PETRUCIO M. Aplicação de um protocolo de avaliação rápida da diversidade de habitats em atividades de ensino e pesquisa (MG-RJ). Acta Limnol Bras. 2002;14:91–98.

CASSANEGRO MBB, DROSTE A. Assessing the spatial pattern of a river water quality in southern Brazil by multivariate analysis of biological and chemical indicators. Braz. J. Biol. 2016;77:118–126.

COSTA TC, BRITO KCT, ROCHA JAV, LEAL KA, RODRIGUES MLK, MINELLA JPG et al. Runoff of genotoxic compounds in river basin sediment under the influence of contaminated soils. Ecotoxicol Environ Saf. 2012;75:63–72.

DALZOCHIO T, SIMÕES LAR, SOUZA MS, RODRIGUES GZP, PETRY IE, ANDRIGUETTI NB et al. Water quality parameters, biomarkers and metal bioaccumulation in native fish captured in the Ilha River, southern Brazil. Cremosphere. 2017;189:609–618.

DALZOCHIO T, RODRIGUES GZP, SIMÕES LAR, SOUZA MS, PETRY IE, ANDRIGUETTI NB et al. In situ monitoring of the Sinos River, southern Brazil: water quality parameters, biomarkers, and metal bioaccumulation in fish. Environ Sci Pollut Res. 2018;25:9485–9500.

DATTA S, SINGH J, SINGH J, SINGH S, SINGH S. Assessment of genotoxic effects of pesticide and vermicompost treated soil with Allium cepa test. Sustain. Environ. Res. 2018;28:171–178.

DEBNATH P, MONDAL A, HAJRA A, DAS C, MONDAL NK. Cytogenetic effects of silver and gold nanoparticles on Allium cepa roots. J Genet Eng Biotechnol. 2018;16:519–526

DREWS E. Comitê define enquadramento da bacia do Rio dos Sinos. Jornal NH [Internet]. 2014 march 31 [cited 2019 sep 20]. Região. Available from: https://www.jornalnh.com.br/_conteudo/2014/03/noticias/regiao/30776-comite-define-enquadramento-da-bacia-do-rio-dos-sinos.html.

DÜSMAN E, ALMEIDA IV, PINTO EP, LUCCHETTA L, VICENTINI VEP. Influence of processing and storage of integral grape juice (Vitis labrusca L.) on its physical and chemical characteristics, cytotoxicity, and mutagenicity in vitro. Genet Mol Res. 2014;16:1–12.

EXTRA CLASSE [Internet]. Um rio de lama. [cited 2019 sep 27]. Ambiente. 2011. Available from: https://www.extraclasse.org.br/ambiente/2011/09/um-rio-de-lama/.

FERNANDES TCC, MAZZEO DEC, MARIN-MORALES MA. Mechanism of micronuclei formation in polyploidizated cells of Allium cepa exposed to trifluralin herbicide. Pestic Biochem Physiol. 2007;88:252–259.

GURGEL PM, NAVONI JA, FERREIRA DM, AMARAL VS. Ecotoxicological water assessment of an

estuarine river from the Brazilian Northeast, potentially affected by industrial wastewater discharge. Sci Total Environ. 2016;572:324–332.

HAQ I, KUMAR S, RAJ A, LOHANI M, SATYANARAYANA GNV. Genotoxicity assessment of pulp and paper mill effluent before and after bacterial degradation using Allium cepa test. Cremosphere. 2017;169:642–650.

HECK TM, JESUS LF, DEUS NT, LINDEN R, OSORIO DMM, STAGGEMEIER R. Avaliação da água através de parâmetros microbiológicos e físico-químicos em áreas populacionais do arroio Luiz Rau, afluente do Rio dos Sinos, município de Novo Hamburgo, RS. Rev. Conhecimento Online [Internet]. 2017 [cited 2019 oct 15];2:105–117. Available from: https://periodicos.feevale.br/seer/index.php/revistaconhecimentoonline/article/view/1146/1836.

INSTITUTO BRASILEIRO DE GEOGRAFIA E ESTATÍSTICA [Internet]. Indicadores de Desenvolvimento Sustentável e Brasil [cited 2019 sep 21]. Síntese de indicadores 2010. Available from: http://www.ibge.gov.br/home/geociencias/recursosnaturais/ids/default_20 10.shtm.

KIENZLER A, MAHLER BJ, METRE PCV, SCHWEIGERT N, DEVAUX A, BONY S. Exposure to runoff from coal-tar-sealed pavement induces genotoxicity and impairment of DNA repair capacity in the RTL-W1 fish liver cell line. Sci Total Environ. 2015;520:73–80.

LEME DM, ANGELIS DF, MARIN-MORALES MA. Action mechanisms of petroleum hydrocarbons present in waters impacted by an oil spill on the genetic material of Allium cepa root cells. Aquat. Toxicol. 2008;88:214–219.

LEME DM, MARIN-MORALES MA. Allium cepa test in environmental monitoring: a review on its application. Mutat Res. 2009;682:71–81.

MACHADO AB, RODRIGUES GZP, SOUZA FG, LINDEN R, OSORIO DMM. Environmental assessment of a stream located in Novo Hamburgo, RS. Rev. Geama [Internet]. 2018 [cited 2019 nov 15];4(2):31–35. Available from: http://www.journals.ufrpe.br/index.php/geama/article/view/1940/482482562.

MARTINS MNC, SOUZA VV, SOUZA TS. Cytotoxic, genotoxic and mutagenic effects of sewage sludge on Allium cepa. Cremosphere. 2016;148:481–486.

MANZANO BC, ROBERTO MM, HOSHINA MM, MENEGÁRIO AA, MARIN-MORALES MA. Evaluation of the genotoxicity of waters impacted by domestic and industrial effluents of a highly industrialized region of São Paulo State, Brazil, by the comet assay in HTC cells. Environ Sci Pollut Res. 2015;22:1399–1407.

MINISTÉRIO DO MEIO AMBIENTE; Conselho Nacional do Meio Ambiente-CONAMA. Resolução N° 357 – Dispõe sobre a classificação dos corpos de água e diretrizes ambientais para o seu enquadramento, bem como estabelece as condições e padrões de lançamento de efluentes, e dá outras providências. Ministério do Meio Ambiente; 2005.

NASCIMENTO CA, STAGGEMEIER R, BIANCHI E, RODRIGUES MT, FABRES R, SOLIMAN MC et al. Monitoring of metals, organic compounds and coliforms in water catchment points from the Sinos River basin. 2015;75 Suppl 2:S50–S56.

OZAKCA DU, SILAH H. Genotoxicity effects of Flusilazole on the somatic cells of Allium cepa. Pestic Biochem Physiol. 2013;107:38–43.

PREFEITURA MUNICIPAL DE NOVO HAMBURGO [Internet]. Novo Hamburgo: passado e futuro. [cited 2019 oct 22]. Available from: http://www.novohamburgo.rs.gov.br/modules/catasg/novohamburgo.

PETRY CT, COSTA GM, BENVENUTTI T, RODRIGUES MAS, DROSTE A. Avaliação integrada da qualidade química e da genotoxicidade da água do arroio Luiz Rau, no trecho inferior da Bacia do Rio dos Sinos, no Sul do Brasil. Rev. Ambient. Água [Internet]. 2016 [cited 2019 nov 12];11:867–877.

RODRIGUES GZP, DALZOCHIO T, GEHLEN G. Uso do bioensaio com Allium cepa L. e análises físico-químicas e microbiológicas para avaliação da qualidade do Rio da Ilha, RS, Brasil. Acta Toxicol. Argent. 2016;24:97–104.

RIBEIRO LR. Teste do micronúcleo em medula óssea de roedores in vivo. Mutagênese ambiental, Ulbra, Canoas. 2003:201–219.

SETH CS, MISRA V, CHAUHAN LK, SINGH RR. Genotoxicity of cadmium on root meristem cells of Allium cepa: cytogenetic and comet assay approach. Ecotoxicol Environ Saf. 2008;71:711–716.

STAPULIONYTÉ A, KLEIZAITÉ V, SIUKTSA R, ZVINGILA D, TARASKEVICIUS R, CESNIENÉ T. Cyto/genotoxicological evaluation of hot spots of soil pollution using Allium bioassays in relation to geochemistry. Mutat Res Genet Toxicol Environ Mutagen. 2019;842:102–110.

YADAV A, RAJ A, PURCHASE D, FERREIRA LFR, SARATALE GD, BHARAGAVA RN. Phytotoxicity, cytotoxicity and genotoxicity evaluation of organic and inorganic pollutants rich tannery wastewater from a Common Effluent Treatment Plant (CETP) in Unnao district, India using Vigna radiata and Allium cepa. Cremosphere. 2019;224:324–332.

ZEGURA B, HEATH E, ERNSA A, FILIPI M. Combination of in vitro bioassays for the determination of cytotoxic and genotoxic potential of wastewater, surface water and drinking water samples. Cremosphere. 2009;75:1453–1460.




DOI: https://doi.org/10.5902/2179460X41818

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