Microbiological Analysis of Surfaces in a Surgical Center: Identification and Bacterial Activity Against Antibiotics and Disinfectant

Fabíola Dresch, Claudete Rempel, Mônica Jachetti Maciel


The objective was to identify the microbiota present on hospital rooms surfaces of a surgical center, to know the susceptibility of the bacteria to antimicrobials and to evaluate the bacterial activity against the disinfectant commonly used in the hospital routine. This is a cross-sectional, descriptive study with a quantitative approach. The samples were collected from surfaces in different environments of the surgical center including admission room, recovery room and operating rooms. The microorganisms were identified and after the isolation, the antimicrobial susceptibility test was performed by the disc-diffusion method. The antimicrobial activity of the disinfectant was evaluated by the quantitative suspension test. Twenty-four areas were analyzed, 15 (62.5%) presented microbial contamination, and 35 strains with a coagulase-negative Staphylococcus coagulase (69%) were isolated, followed by S. saprophyticus (23%), Acinetobacter sp. (6%) and E. coli (2%). Of these, 51% had resistance to at least one antibiotic, and Staphylococcus methicillin resistant strains were found. The tested disinfectant showed proven antimicrobial activity. The antimicrobial action of the disinfectant was proven, but the presence of microorganisms evidences the importance of hygienic care in order to avoid the recurrence of contamination after cleaning.


Operating Rooms; Disinfectant; Hospital management

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ALEXANDER JW, VAN SWERINGEN H, VANOSS K, HOOKER EA, EDWARDS MJ. Surveillance of bacterial colonization in operating rooms. Surgical infections. 2013;14(4):345-351.

AMARAL AM, DIOGO FILHO A, SOUSA MM, BARBOSA PA, GONTIJO FILHO PP. The importance of protecting surgical instrument tables from intraoperative contamination in clean surgeries. Revista Latino-Americana de Enfermagem. 2013;21(1):426–432.

BARRETO MF, PICOLI SU. Staphylococcus in a hospital in Porto Alegre (RS). Revista Brasileira de Análises Clínicas. 2008;40(4):285-287.

BERNARDI, ADILSON CÉSAR ABREU; PIZZOLITTO, ELISABETH LOSHCHAGIN; PIZZOLITTO, ANTONIO CARLOS. Detecção da produção de slime por estafilococos coagulase-negativa isolados de cateter venoso central. Rev. Ciênc. Farm. Básica Apl. 2007;28(1):57-66.

BHALLA A, PULTZ NJ, GRIES DM, RAY AJ, ECKSTEIN EC, ARON DC et al. Acquisition of nosocomial pathogens on hands after contact with environmental surfaces near hospitalized patients. Infection Control & Hospital Epidemiology. 2004;25(2):164-167.

BRASIL. Agência Nacional de Vigilância Sanitária (ANVISA). Microbiologia Clínica para o Controle de Infecção Relacionada à Assistência à Saúde. Módulo 6: Detecção e identificação de bactérias de importância médica. 1 ed. Brasília: Anvisa. 2013.

BRASIL. Agência Nacional de Vigilância Sanitária (ANVISA). Sítio cirúrgico: Critérios Nacionais de Infecções relacionadas à assistência à saúde. Brasília: Anvisa. 2009. Disponível em: http://www.anvisa.gov.br/servicosaude/manuais/criterios_nacionais_ISC.pdf.

BREATHNACH AS. Nosocomial infections and infection control. Medicine. 2013;41(11):649-653.

BS - British Standard. The European Standard EN 1040:2005 has. Chemical disinfectants and antiseptics – quantitative - suspencion test for the evaluation of basic bactericidal activity of chemical desinfectants and antiseptics –Test method and requerements (phase 1), 2006.

CLSI - Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing: Twenty-Fourth Informational Supplement. M100 S-24.2014;34(1). Disponível em: http://ncipd.org/control/images/NCIPD_docs/CLSI_M100-S24.pdf

HIRZEL W. Implicancias del Staphylococcus saprophyticus en la patología infecciosa urinaria de la mujer. Acta Bioquímica Clínica Latino Americana [online]. 2004;38(2):217-222.

LABORCLIN. Manual do Antibiograma; 2013. 32 p. Disponível em http://www.interlabdist.com.br/dados/noticias/pdf_238.pdf

LANGSRUD S, SUNDHEIM G, BORGMANN-STRAHSEN R. Intrinsic and acquired resistance to quaternary ammonium compounds in food-related Pseudomonas spp. J Appl Microbiol. 2003;95:874-882.

LOFTUS RW, KOFF MD, BURCHMAN CC, SCHWARTZMAN JD, THORUM V, READ ME, WOOD TA, BEACH ML. Transmission of pathogenic bacterial organisms in the anesthesia work area. Anesthesiology. 2008;109(3):399-407.

LYNCH RJ, ENGLESBE MJ, STURM L, BITAR A, BUDHIRAJ K, KOLLA S et al. Measurement of foot traffic in the operating room: implications for infection control. American Journal of Medical Quality. 2009;24(1):45-52.

MANGRAM AJ, HORAN TC, PEARSON ML, SILVER LC, JARVIS WR. Guideline for prevention of surgical site infection, 1999. Infection control and hospital epidemiology. 1999;20(4):247-278.

ROSENTHAL VD, GUZMAN S, ORELLANO PW. Nosocomial infections in medical-surgical intensive care units in Argentina: attributable mortality and length of stay. American Journal of Infection Control. 2003;31(5): 291-295.

SANTOS NQ. A resistência bacteriana no contexto da infecção hospitalar. Texto Contexto Enfermagem. 2004;13(n.esp):64-70.

SAVAGE RD, FOWLER RA, RISHU AH, BAGSHAW SM, COOK D, DODEK P et al. Pathogens and antimicrobial susceptibility profiles in critically ill patients with bloodstream infections: a descriptive study. CMAJ Open. 2016; 4(4):E569-E577.

SRISUNG W, TEERAKANOK J, TANTRACHOTI P, KARUKOTE A, NUGENT K. Surgical prophylaxis with gentamicin and acute kidney injury: a systematic review and meta-analysis. Ann Transl Med. 2017;5(5):100

URBAN C, SEGAL-MAURER S, RAHAL J. Considerations in control and treatment of nosocomial infections due to multidrug-resistant Acinetobacter baumannii. Clinical Infectious Diseases. 2003;36(10):1268-1274.

WEBER DJ, RUTALA WA, MILLER MB, HUSLAGE K, SICKBERT-BENNETT E. Role of hospital surfaces in the transmission of emerging health care-associated pathogens: norovirus, Clostridium difficile, and Acinetobacter species. American Journal of Infection Control. 2010;38(5 Suppl 1):25-33.

YEZLI S, BARBUT F, OTTER JA. OTTER. Surface contamination in operating rooms: a risk for transmission of pathogens? Surgical infections. 2014;15(6):694-69.

DOI: http://dx.doi.org/10.5902/2179460X26881


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