Evaluating the copper water Uruguay river mutagenic potential
DOI:
https://doi.org/10.5902/2179460X13610Keywords:
Ecotoxicology. Micronuclei. Poluttion. Zebrafish.Abstract
http://dx.doi.org/10.5902/2179460X13610
Analysis of Uruguay river water noted the presence of compounds at concentrations above the limits recommended by the brazilian legislation, such as the copper. Therefore, this study aimed to evaluate the mutagenic potential of copper conducting exposure of bioindicator species Danio rerio to the metal in bioassay systems. After acclimation period, thirty-six animals were divided into three groups, with rejoinders: (a) Control (without copper), (b) T1 (with 0.08 mg/L - concentration observed in the river) and (c) T2 (with 0.009 mg/L - concentration recommended by CONAMA). After 120 hours of exposure, we proceeded to collect blood for the piscine micronucleus test, where 2000 cells were analyzed per individual, the result being statistically analyzed using the Kruskal-Wallis test. Were observed highly significant differences (p <0.01) among the three study groups, with a higher frequency of micronuclei in T1 compared with the control (p = 0.0079) and T2 (p = 0.0027). Thus, this study suggests possible consequences of copper contamination to the local fish fauna of this important water resource, used both as a source of water to several municipalities and for extractive fishing.
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