Atividade antibacteriana de Brassica oleracea var. Capitata em modelos experimentais in vitro
DOI:
https://doi.org/10.5902/223613086427Keywords:
Anti-Bacterial Agents, Plants Medicinal, Medicine Traditional, Phytotherapy, BrassicaAbstract
The ability of microorganisms, especially bacteria become resistant, has been widelydiscussed in various publications, and plants are one source to search for new drugs that each dayhas become more necessary. This work aimed to evaluate different ways of preparing extracts ofBrassica oleracea var. capitata and test them fronts strain of Staphylococcus aureus using the teston solid medium by diffusion in agar and in liquid medium in the ELISA plate. Tests revealed thatextracts obtained by solvent extraction and decoction hydroalcoholic acid have greater appeal tothe search for new drugs with activity against S. aureus, and the latter fractions obtained by liquidliquidpartition relating to solvents ethyl acetate, dichloromethane and hydroalcoholic remainingfraction showed more efficient. As for the solid medium, there was a lack of antibacterial activityof hydroalcoholic extract. However, a strong antibacterial activity was observed when analyzed inthe same extracts in liquid medium. Ethanolic extracts with concentrations of 50 and 70% showedbetter results when evaluated for 8 h of incubation. Different results were observed when theperiod was bent where the concentrations became more expressive of the 70 and 80%. The resultsdemonstrate that the species is a potential source of antibacterial agents. However, the choice ofsolvent, the extraction method and the bacterial growth medium should be considered. The datashown here motivates further studies for the isolation and identification of the active principlesresponsible for the antibacterial activity that can be used in the pharmaceutical industry, becauseof the large number of resistance to existing antibacterial drugs.Downloads
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Klein T, Longhini R, Bruschi ML et al. Fitoterápicos: um mercado promissor. Revista Ciência Farmacêutica Básica Aplicada, 2009, 30(3):241-248.
Barreto F, Sousa E, Campos A et al. Antibacterial Activity of Lantana camara Linn and Lantana montevidensis Brig Extracts from Cariri-Ceará, Brazil. Journal of Young Pharmacists, 2010, 2(1):42-44.
Höfling JF, Anibal PC, Obando-Pereda GA et al. Antimicrobial potential of some plant extracts against Candida species. Brazilian Journal of Biology, 2010, 70(4):1065-1068.
Ponzi EAC, Oliveira TL, Morais IAF et al. Antimicrobial activity of the Momordica charantia L. extract. Revista de Cirurgia e Traumatologia Buco-Maxilo-facial, 2010, 10(1):89-94.
Lorenzi H, Matos FJ De A. Plantas medicinais no Brasil/Nativas e exóticas. 1.ed. Nova Odessa, Instituto Plantarum, 2002:512.
Jain P, Bansal D, Bhasin P. Antibacterial activity of aqueous plant extracts against Escherichia coli and Bacillus subtilis. Drug Invention Today, 2010, 2(4):220-222.
Costa JGM, Rodrigues FFG, Angélico EC et al. Estudo químico-biológico dos óleos essenciais de Hyptis martiusii, Lippia sidoides e Syzigium aromaticum frente a larvas do Aedes aegypti. Revista Brasileira de Farmacognosia, 2005, 15(4):304-309.
Oliveira TG, Carvalho CA, Dal’piva GG et al. Chromatographic and spectrophotometric profiles of different phenologic stages of Brassica oleracea var. capitata. Revista Eletrônica de Farmácia, 2007, 4(2):142-152.
Monsalve C, Cano A. La Família Brassicaceae em la província de Huaylas, Áncash. Revista Peruana de Biologia, 2003, 10(1):20-32.
Fracaro F, Sartori M, Bizzani E et al. Agronomic Behavior of Cabbage Cultivars and Hybrids in the Northeast Region of Rio Grande do Sul, Brasil. Revista de Ciência Rural, 1999, 29(3):465-468.
Filgueira FAR. Novo Manual de Olericultura: agrotecnologia moderna na produção e comercialização de hortaliças. 3.ed. Viçosa, UFV, 2000:421.
Carvalho CA, Silva MB, Oliveira TG et al. Estudo espectrométrico de diferentes estágios fenológicos da Brassica oleracea var. capitata. Revista Brasileira de Farmacognosia, 2008, 18(2):249-257.
Sarandy MM. Avaliação do efeito cicatrizante do extrato de repolho (Brassica oleraceae var. capitata) em ratos Winstar. 2007. 57p. Dissertação para o Programa de Pós Graduação em Biologia Celular e Estrutural, Universidade Federal de Viçosa, Viçosa.
Cheney G. Vitamin U therapy of pepticulcer. California Medicine, 1952, 77(4):248–252.
Ranocha P, Mcneil SD, Ziemak MJ et al. The S-methylmethionine cycle in angiosperms: ubiquity, antiquity and activity. The Plant Journal, 2001, 25(5):575–584.
Augspurger NR, Scherer CS, Garrow TA et al. Dietary S-Methyl methionine, a component of foods, has choline-sparing activity in chickens. Journal of Nutrition, 2005, 135, 1712–1717.
Carvalho CA, Fernandes KM, Matta SLP et al. Evaluation of antiulcerogenic activity of aqueous extract of Brassica oleracea var. capitata (cabbage) on Wistar rat gastric ulceration. Experimental Gastroenterology, 2011, 48(4), p. 276-288.
Simões CMO, Schenkel EP, Gosmann G et al. Farmacognosia: da planta ao medicamento. 5.ed. Porto Alegre/Florianópolis, Editora da UFRGS/Editora da UFSC, 2004:1102.
Balbach A, Boarim D. As Hortaliças na Medicina Natural. 2.ed. Itaquacetuba, Vida Plena, 1993:280.
Gnoatto SCB, Bassani VL, Coelho GC et al. Influência do método de extração nos teores de metilxantinas em erva-mate (Ilex paraguariensis a. st.-Hil., aquifoliaceae). Química Nova, 2007, 30(2), 304-307.
Astill C, Birch MR, Dacombe C et al. Factors affecting the caffeine and polyphenol contents of black and Green tea infusions. Journal of Agricultural and Food Chemistry, 2001, 49(11), 5340-5347.
Silva IDD, Aragão CFS. Avaliação de parâmetros de extração da Cinchona Vahl por métodos farmacopéicos e não farmacopéicos. Revista Brasileira de Farmacognosia, 2009, 19(3), 776-780.
Longhini R, Raksa SM, Oliveira ACP et al. Obtenção de extratos de própolis sob diferentes condições e avaliação de sua atividade antifúngica. Revista Brasileira de Farmacognosia, 2007, 17(3), 388-395.
Filho VC, Yunes RA. Estratégias para a obtenção de compostos farmacologicamente ativos a partir de plantas medicinais. Conceitos sobre modificação estrutural para otimização da atividade. Quimica Nova, 1998, 21(1), 99-105.
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