MORPHO-ANATOMIC OF ADVENTITIOUS RHIZOGENESIS IN MINI-CUTTINGS OF <i>Eucalyptus grandis</i> x <i>Eucalyptus urophylla</i>
DOI:
https://doi.org/10.5902/1980509815721Keywords:
mini-cuttings, vegetative propagation, anatomy.Abstract
http://dx.doi.org/10.5902/1980509815721
The present work aimed to accomplish the histological analysis of the events involved in the adventitious rooting pattern of four Eucalyptus grandis x Eucalyptus urophylla clones. The mini-cuttings were collected from a mini-stump and the rooting experiments were set up under mini-hedge indoor semi-hydroponics by means of intermittent flooding fertirrigation. For histological analyses, the proximal ends of the mini-cuttings were collected after 0, 1, 2, 4, 6, 8, 10, and 12 days after planting them at greenhouse. After fixation with FAA70%, the samples were dehydrated in a graded ethanol series and infiltrated overnight in metracrilate resin, and finally embedded in resin. It was verified the endogenous origin of adventitious root primordia from vascular cambium, and in some cases, the formation and proliferation of calli at the proximal end of the mini-cuttings within 8 to 12 days.
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