EFFECT OF FREQUENCY OF IMMERSION AND AIR INJECTION ON IN VITRO MULTIPLICATION OF Eucalyptus grandis x Eucalyptus urophylla IN TEMPORARY IMMERSION BIOREACTOR
DOI:
https://doi.org/10.5902/1980509813321Keywords:
In vitro propagation, cloning, gaseous atmosphere, hyperhydricity.Abstract
The objectives of this study were to evaluate different immersion frequencies (2, 4, 8 and 16 h) andexplants supports (filter paper and foam) of a Eucalyptus grandis x Eucalyptus urophylla clone insideRITA® bioreactors; and a ventilation system with additional air input coupled to the bioreactor containers.It was evaluated the explants fresh weight, number of shoots and hyperhydricity and in vitro axillary budmultiplication. The immersions every two and four hours and the filter paper showed higher growth andnumber of shoots, but caused a greater percentage of hyperhydric shoots. Under the conditions and systemtested, the additional air injection in the RITA® bioreactor did not influence the culture growth.Downloads
References
ABDOLI, M.; MOIENI, A.; DEHGHANI, H. Effects of cultivar and agar concentration on in vitro shoot organogenesis and hyperhydricity in sunflower (Helianthus annuus L.). Pakistan Journal of Botany, Islamabad, v. 39, n. 1, p. 31-35, 2007.
AFREEN, F. Temporary immersion bioreactor – Engineering considerations and applications in plant micropropagation. In: GUPTA, S. D.; IBARAKI, Y. (Eds.). Plant tissue culture engineering. Dordrecht: Springer, 2006. p. 187-201.
BRAND, M. H. Agar and ammonium nitrate influence hyperhydricity, tissue nitrate and total nitrogen content of serviceberry (Amelanchier arborea) shoots in vitro. Plant Cell, Tissue and Organ Culture, Dordrecht, v. 35, p. 203-209, 1993.
CASTRO, D. R.; GONZÁLEZ, O. J. Micropropagación de eucalipto (Eucalyptus grandis Hill ex Maiden) en el sistema de inmersión temporal. Agricultura Técnica, Chillán, v. 62, p. 68-78, 2002.
CHAKRABARTY, D. et al. Micropropagation of apple root stock ‘M9 EMLA’ using bioreactor. Journal of Horticultural Science and Biotechnology, v.78, p. 605-609, 2003.
CHAKRABARTY, D. et al. Hyperhydricity in apple: ultrastructural and physiological aspects. Tree Physiology, v. 26, p. 377-388, 2005.
CORREIA, A. C. G. Micropropagação em biorreatores de imersão temporária e enraizamento de miniestacas e microestacas de clones híbridos de Eucalyptus globulus. 2011, 62 p. Dissertação (Mestrado em Ciência Florestal) – Universidade Federal de Viçosa, Viçosa, MG.
ETIENNE, H.; BERTHOULY, M. Temporary immersion system in plant micropropagation. Plant Cell, Tissue and Organ Culture, Dordrecht, v. 69, p. 215-231, 2002.
GASPAR, T. et al. Vitrification: morphological, physiological and ecological aspects. In: BONGA, J. M.; DURZAN, D. J. (Eds.). Cell and tissue culture in forestry. Dordrecht: Martinus Nijhoff Publishers, 1987. v. 1, p. 152-166.
GASPAR, T. Vitrification in micropropagation. In: BAJAJ, Y. P. S. (Ed.). Biotechnology in agriculture and forestry – High-tech and micropropagation I. Berlin: Springer, 1991. v. 17, p. 116-126.
HARTMANN, H. T. et al. Plant propagation: principles and practices. 6. ed. New Jersey: Prentice-Hall, 2002. 770 p.
LAI, C. et al. Hyperhydricity in shoot cultures of Scrophularia yoshimurae can be effectively reduced by ventilation of culture vessels. Journal of Plant Physiology, v. 162, p. 355-361, 2005.
LEMOS, E. E. P. et al. Micropropagação de clones de banana cv. Terra em biorreator de imersão temporária. Revista Brasileira de Fruticultura, Jaboticabal, v. 23, n. 3, p. 482-487, dez., 2001.
MAJADA, J. P. et al. Impact of culture vessel ventilation on the anatomy and morphology of micropropagated carnation. Plant Cell, Tissue and Organ Culture, Dordrecht, v. 63, p. 207-214, 2000.
MARGA, F.; VEBRET, L.; MORVAN, H. Agar fractions could protect apple shoots cultured in liquid media against hyperhydricity. Plant Cell, Tissue and Organ Culture, Dordrecht, v. 49, p. 1-5, 1997.
MAYOR, M. L. et al. A. Reduction of hyperhydricity in sunflower tissue cultures. Plant Cell, Tissue and Organ Culture, Dordrecht, v. 72, p. 99-103, 2003.
McALISTER, B. et al. Use of the temporary immersion bioreactor system (RITA) for production of commercial Eucalyptus clones in Mondi Forest (SA). Plant Cell, Tissue and Organ Culture, Dordrecht, v. 81, p. 347-358, 2005.
MURASHIGE, T.; SKOOG, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, Copenhagen, v. 15, p. 473-497, 1962.
PARK, S. W. et al. Effect of sealed and vented gaseous microenvironments on the hyperhydricity of potato shoots in vitro. Scientia Horticulturae, Amsterdam, v. 99, p. 199-205, 2004.
PICOLI, E. A. T. et al. Ultrastructural and biochemical aspects of normal and hyperhydric eucalypt. International Journal of Horticultural Science, Budapest, v. 14, n. 3, p. 61-69, 2008.
REIS, J. P. et al. Micropropagação de eucalipto no sistema de imersão temporária. In: CONGRESSO BRASILEIRO DE CULTURA DE TECIDOS DE PLANTAS, 1., 2003, Lavras. Anais... Lavras: UFLA/FAEPE, 2003. p. 276.
RODRIGUES, P. H. V.; TEIXEIRA, F. M.; LIMA, A. M. L. P.; Ambrosano, G. M. B. Propagação de mudas de helicônia em biorreator de imersão temporária. Bragantia, Campinas, v. 65, n. 1, p. 29-35, 2006.
SAHER, S. et al. Prevention of hyperhydricity in micropropagated carnation shoots by bottom cooling: implications of oxidative stress. Plant Cell, Tissue and Organ Culture, Dordrecht, v. 81, p. 149-158, 2005.
SILVA, A. B. et al. Métodos de propagação do abacaxizeiro. Pesquisa Agropecuária Brasileira, Brasília, v. 42, n. 9, p. 1257-1260, 2007.
TAKAYAMA, S.; AKITA, M. Bioengineering aspects of bioreactor application in plant propagation. In: GUPTA, S. D.; IBARAKI, Y. (Eds.). Plant tissue culture engineering. Dordrecht: Springer. p. 83-100, 2006.
WHITEHOUSE, A. B.; MARKS, T. R.; EDWARDS, G. A. Control of hyperhydricity in Eucalyptus axillary shoot cultures grown in liquid medium. Plant Cell, Tissue and Organ Culture, Dordrecht, v. 71, p. 245-252, 2002.
YADAV, M. K.; GAUR, A. K.; GARG, G. K. Development of suitable protocol to overcome hyperhydricity in carnation during micropropagation. Plant Cell, Tissue and Organ Culture, Dordrecht, v. 72, p. 153-156, 2003.
ZIV, M. The control of biorreactor environment for plant propagation in liquid culture. Acta Horticulturae, v. 393, p. 25-38, 1995.
ZIV, M. Vitrification: morphological and physiological disorders of in vitro plants. In: DEBERGH, P. C.; ZIMMERMAN, R H. (Eds.). Micropropagation, Technology and Application. Dordrecht: Kluwer Academic Publishers, 1991. p. 45-69.
