Optimization of production and partial characterization of xylanase from a newly isolated Bacillus amyloliquefaciens

Autores

DOI:

https://doi.org/10.5902/2179460X42741

Palavras-chave:

xylanase, bacillus amyloliquefaciens, optimization

Resumo

This paper reports the process of production optimization and partial characterization of xylanase from a newly isolated Bacillus amyloliquefacies VR002, isolated from local soil. The microorganism exhibited maximum xylanase production when 1.0% (v/v) of inoculum size was added to culture medium with initial pH 6, 1.0% (w/v) birchwood xylan, at 35 °C after 48h of incubation. Xylanase production in different carbon sources apart from birchwood xylan and xylose did not show high production levels. Optimum pH for xylanase activity was 6.0. The enzyme was alkali-stable and retained 100% of residual activity over the pH range from 6.0 to 10.0 for 24 h at 25°C. Optimum temperature for enzyme activity was 55°C. Xylanase was 100% stable at 4°C and 25°C even after 24h of incubation, a desirable characteristic for enzyme storage. Moreover, best crude extract volume and time reaction were found to be 10 µL and 5 min, respectively. After optimization of production and activity parameters, an increase of nearly 60-fold in xylanase activity (44.12 ± 4.36 U/mL) was achieved. Characteristics of B. amyloliquefaciens VR002 xylanase are particularly desirable for biotechnological applications

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Biografia do Autor

Bruno Las-Casas Chaves, Universidade Federal Fluminense

Graduation student of Agribusiness Engineering at Universidade Federal Fluminense. He works on scientific Initiation project, studying the optimization of microbial production of the enzyme xylanase. He have studied Biotechnology at Universidade da Beira Interior (Portugal) during one semester.

Ana Paula Martinazzo, Universidade Federal Fluminense

Bachelor's at Agricultural Engineering from Universidade Estadual do Oeste do Paraná (1995), master's at Agricultural Engineering from Universidade Federal de Viçosa (1997) and doctorate at Agricultural Engineering from Universidade Federal de Viçosa (2006). Has experience in Agricultural Engineering, focusing on Engineering of Processing of Agricultural Products, acting on the following subjects: secagem, plantas medicinais, óleo essencial, grãos and cymbopogon citratus.

Brisabella Coca, Universidade Federal Fluminense

She has graduation at Biological Science by Centro Universitário de Barra Mansa (2005) and specialization at Environmental Science by Centro Universitário de Barra Mansa (2007).  Currently she works at Porto Real’s City Hall.  She has experience with General Biology.

Adriane Nunes de Souza, Universidade Salgado de Oliveira

Graduated in Biological Sciences at the Universidade Estadual do Norte Fluminense Darcy Ribeiro (1997), Graduated in Teacher Training _ Full Degree in Biology at Universidade Salgado de Oliveira (2011), Master in Plant Production at Universidade Estadual do Norte Fluminense Darcy Ribeiro (2000) and PhD in Science from the Center for Nuclear Energy in Agriculture - University of São Paulo (2005) and Post Doctorate in Biosciences and Biotechnology from the State University of Norte Fluminense Darcy Ribeiro (2009). Currently, I work as Full Professor at Salgado de Oliveira University. Member of the NDE of the Nursing course - UNIVERSO and of the Collegiate of the Nutrition course - Universe.She has graduation at Biological Science by Centro Universitário de Barra Mansa (2005) and specialization at Environmental Science by Centro Universitário de Barra Mansa (2007).  Currently she works at Porto Real’s City Hall.  She has experience with General Biology.

Carlos Eduardo Teodoro, Universidade Federal Fluminense

Bachelor’s at Agronomy by Universidade Federal Rural do Rio de Janeiro (1997), master's at Crop Production by Universidade Estadual do Norte Fluminense Darcy Ribeiro (1999) and Ph.D. at Bioscience and Biotechnology by Universidade Estadual do Norte Fluminense Darcy Ribeiro (2004) . Currently he is a professor at Universidade Federal Fluminense and reviewer of Environmental and Agricultural Engineering Brazilian Journal.

Referências

ADHYARU, Dharmesh N. et al. Cellulase-free-thermo-alkali-solvent-stable xylanase from Bacillus altitudinis DHN8: Over-production through statistical approach, purification and bio-deinking/ bio-bleaching potential. Biocatalysis and Agricultural Biotechnology, [S. l.], v. 12, p. 220–227, 2017. Disponível em: https://doi.org/10.1016/j.bcab.2017.10.010

AKHAVAN SEPAHY, Abbas; GHAZI, Shokoofeh; AKHAVAN SEPAHY, Maryam. Cost-effective production and optimization of alkaline xylanase by indigenous Bacillus mojavensis AG137 fermented on agricultural waste. Enzyme Research, [S. l.], v. 2011, n. 1, 2011.

ALTAF, Simair A. et al. Characterization of Crude Xylanase Produced by Edible Mushroom Pleurotus eryngii. Journal of bioprocessing and biotechniques, [S. l.], v. 6, n. 2, p. 6–11, 2016.

BAJAJ, Bijender Kumar; MANHAS, Kalpana. Production and characterization of xylanase from Bacillus licheniformis P11(C) with potential for fruit juice and bakery industry. Biocatalysis and Agricultural Biotechnology, [S. l.], v. 1, n. 4, p. 330–337, 2012. Disponível em: http://dx.doi.org/10.1016/j.bcab.2012.07.003

BATTAN, Bindu et al. Enhanced production of cellulase-free thermostable xylanase by Bacillus pumilus ASH and its potential application in paper industry. Enzyme and Microbial Technology, [S. l.], v. 41, n. 6–7, p. 733–739, 2007.

BÉRENGER, Jean-François et al. Production, purification, and properties of thermostable xylanase from Clostridium stercorarium . Canadian Journal of Microbiology, [S. l.], v. 31, n. 7, p. 635–643, 2010.

BERNIER, Roger et al. Isolation and Characterization of a Xylanase from Bacillus subtilis. Applied and environmental microbiology, [S. l.], v. 46, n. 2, p. 511–514, 1983. Disponível em: http://aem.asm.org/content/46/2/511.short

BHALLA, Aditya; BISCHOFF, Kenneth M.; SANI, Rajesh Kumar. Highly Thermostable Xylanase Production from A Thermophilic Geobacillus sp. Strain WSUCF1 Utilizing Lignocellulosic Biomass. Frontiers in Bioengineering and Biotechnology, [S. l.], v. 3, n. June, p. 1–8, 2015.

BOCCHINI, D. .. et al. Optimization of Xylanase Production by Bacillus Circulans D1 in Submerged Fermentation Using Response Surface Methodology. Process Biochemistry, [S. l.], v. 38, n. 5, p. 727–731, 2003.

BOCCHINI, D. A.; GOMES, E.; DA SILVA, R. Xylanase production by Bacillus circulans D1 using maltose as carbon source. Applied Biochemistry and Biotechnology, [S. l.], v. 146, n. 1–3, p. 29–37, 2008.

COUGHLAN, MP; HAZLEWOOD, GP. beta‐1,4‐D‐xylan‐degrading enzyme systems: biochemistry, molecular biology and applications. Biotechnology and Applied Biochemistry, [S. l.], v. 17, n. 3, p. 259–289, 1993.

DEEKER, Robert F. H.; ROBERTS, Geoffrey N. Structures of the oligosaccharides from the enzymic hydrolysis of hemicellulose by a hemicellulase of Ceratocystis paradoxa. Carbohydrate Research, [S. l.], v. 43, p. 335–344, 1975.

DHILLON, Ashita et al. A cellulase-poor, thermostable, alkalitolerant xylanase produced by Bacillus circulans AB 16 grown on rice straw and its application in biobleaching of eucalyptus pulp. Bioresource Technology, [S. l.], v. 73, n. 3, p. 273–277, 2000.

GHOSH, Mousumi et al. Aspergillus sydowii MG 49 is a strong producer of thermostable xylanolytic enzymes. Enzyme and Microbial Technology, [S. l.], v. 15, n. 8, p. 703–709, 1993.

GUPTA, Uma; KAR, Rita. Xylanase production by a thermo-tolerant Bacillus species under solid-state and submerged fermentation. Brazilian Archives of Biology and Technology, [S. l.], v. 52, n. 6, p. 1363–1371, 2009.

HALTRICH, Dietmar et al. Production of fungal xylanases. Bioresource Technology, [S. l.], v. 58, n. 2, p. 137–161, 1996.

HECK, Júlio Xandro; DE BARROS SOARES, Luís Henrique; ZÁCHIA AYUB, Marco Antônio. Optimization of xylanase and mannanase production by Bacillus circulans strain BL53 on solid-state cultivation. Enzyme and Microbial Technology, [S. l.], v. 37, n. 4, p. 417–423, 2005.

IRFAN, Muhammad et al. Optimization of process parameters for xylanase production by Bacillus sp. in submerged fermentation. Journal of Radiation Research and Applied Sciences, [S. l.], v. 9, n. 2, p. 139–147, 2015. Disponível em: http://dx.doi.org/10.1016/j.jrras.2015.10.008

KAPOOR, Mukesh; NAIR, Lavanya M.; KUHAD, Ramesh Chander. Cost-effective xylanase production from free and immobilized Bacillus pumilus strain MK001 and its application in saccharification of Prosopis juliflora. Biochemical Engineering Journal, [S. l.], v. 38, n. 1, p. 88–97, 2008.

KAVYA, Venkatesh; PADMAVATHI, Tallapragada. Optimization of growth conditions for xylanase production by Aspergillus niger in solid state fermentation. Polish Journal of Microbiology, [S. l.], v. 58, n. 2, p. 125–130, 2009.

KHASIN, Alexander; ALCHANATI, Iris; SHOHAM, Yuval. Purification and Characterization of a Thermostable Xylanase from Bacillus stearothermophilus T-6. Applied and Environmental Microbiology, [S. l.], v. 59, n. 6, p. 1725–1730, 1993.

KHUSRO, Ameer et al. Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI. Electronic Journal of Biotechnology, [S. l.], v. 22, p. 16–25, 2016. Disponível em: http://dx.doi.org/10.1016/j.ejbt.2016.04.002

KULKARNI, Neeta; SHENDYE, Abhay; RAO, Mala. Molecular and biotechnological aspects of xylanases. FEMS Microbiology Reviews, [S. l.], v. 23, n. 4, p. 411–456, 1999.

KUMAR, Sharad et al. Purification, characterization and thermostability improvement of xylanase from Bacillus amyloliquefaciens and its application in pre-bleaching of kraft pulp. 3 Biotech, [S. l.], v. 7, n. 1, 2017.

LAMA, Licia et al. Purification and characterization of thermostable xylanase and β-xylosidase by the thermophilic bacterium Bacillus thermantarcticus. Research in Microbiology, [S. l.], v. 155, n. 4, p. 283–289, 2004.

LENGELER, J. W. Catabolite Repression. Brenner’s Encyclopedia of Genetics: Second Edition, [S. l.], p. 447–452, 2013.

LINCOLN, Ralph E. Control of stock culture preservation and inoculum build-up in bacterial fermentation. Journal of Biochemical and Microbiological Technology and Engineering, [S. l.], v. 2, n. 4, p. 481–500, 1960.

MILLER, GL. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry, [S. l.], v. 31, n. 3, p. 426–428, 1959.

MOON, Seung‐Hyeon ‐H; PARULEKAR, Satish J. A parametric study ot protease production in batch and fed‐batch cultures of Bacillus firmus. Biotechnology and Bioengineering, [S. l.], v. 37, n. 5, p. 467–483, 1991.

MURUGAN, Sevanan et al. Production of Xylanase from Arthrobacter sp. MTCC 6915 Using Saw Dust As Substrate under Solid State Fermentation . Enzyme Research, [S. l.], v. 2011, p. 1–7, 2011.

NAGAR, Sushil et al. Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation. Journal of Industrial Microbiology and Biotechnology, [S. l.], v. 37, n. 1, p. 71–83, 2010.

NAGAR, Sushil et al. Production of alkali tolerant cellulase free xylanase in high levels by Bacillus pumilus SV-205. International Journal of Biological Macromolecules, [S. l.], v. 50, n. 2, p. 414–420, 2012. Disponível em: http://dx.doi.org/10.1016/j.ijbiomac.2011.12.026

NASCIMENTO, R. P. et al. Production and partial characterisation of xylanase from Streptomyces sp. strain AMT-3 isolated from Brazilian cerrado soil. Enzyme and Microbial Technology, [S. l.], v. 31, n. 4, p. 549–555, 2002.

POLIZELI, M. L. T. M. et al. Xylanases from fungi: Properties and industrial applications. Applied Microbiology and Biotechnology, [S. l.], v. 67, n. 5, p. 577–591, 2005.

PRAKASH, P. et al. Production of alkaliphilic, halotolerent, thermostable cellulase free xylanase by Bacillus halodurans PPKS-2 using agro waste: Single step purification and characterization. World Journal of Microbiology and Biotechnology, [S. l.], v. 28, n. 1, p. 183–192, 2012.

RANI, D. Swaroopa; NAND, Krishna. Production of thermostable cellulase-free xylanase by Clostridium absonum CFR-702. Process Biochemistry, [S. l.], v. 36, n. 4, p. 355–362, 2000.

RATANAKHANOKCHAI, Khanok; KYU, Khin Lay; TANTICHAROEN, Morakot. Purification and Properties of a Xylan-Binding Endoxylanase from Alkaliphilic Bacillus sp . Strain K-1. Applied and environmental microbiology, [S. l.], v. 65, n. 2, p. 694–697, 1999.

REYMOND, Jean Louis; FLUXÀ, Viviana S.; MAILLARD, Noélie. Enzyme assays. Chemical Communications, [S. l.], v. 1, n. 1, p. 34–46, 2009. Disponível em: http://dx.doi.org/10.1016/j.pisc.2014.02.005

SANGHI, Ashwani et al. One-step purification and characterization of cellulase-free xylanase produced by alkalophilic bacillus subtilis ASH. Brazilian Journal of Microbiology, [S. l.], v. 41, n. 2, p. 467–476, 2010.

SCANDURRA, Roberto et al. Protein thernmstability in extrernophiles. Biochimie, [S. l.], v. 80, n. D, p. 933–941, 1998.

SHAH, Amita R.; MADAMWAR, Datta. Xylanase production by a newly isolated Aspergillus foetidus strain and its characterization. Process Biochemistry, [S. l.], v. 40, n. 5, p. 1763–1771, 2005.

SRINIVASAN, M. C.; RELE, Meenakshi V. Microbial xylanase for paper industry. Current Science, [S. l.], v. 77, n. 1, p. 137–142, 1999.

SUNNA, A.; ANTRANIKIAN, G. Xylanolytic Enzymes from Fungi and Bacteria. Critical Reviews in Biotechnology, [S. l.], v. 17, n. 1, p. 39–67, 2008.

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Publicado

2020-05-11 — Atualizado em 2022-01-13

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Como Citar

Chaves, B. L.-C., Martinazzo, A. P., Coca, B., de Souza, A. N., & Teodoro, C. E. (2022). Optimization of production and partial characterization of xylanase from a newly isolated Bacillus amyloliquefaciens. Ciência E Natura, 42, e9. https://doi.org/10.5902/2179460X42741 (Original work published 11º de maio de 2020)

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v. 42 (2020): CIÊNCIA E NATURA: Edição Comemorativa – 40 ANOS

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Química

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Copyright (c) 2020 Ciência e Natura

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