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Optimization of production and partial characterization of xylanase from a newly isolated Bacillus amyloliquefaciens

Authors

DOI:

https://doi.org/10.5902/2179460X42741

Keywords:

xylanase, bacillus amyloliquefaciens, optimization

Abstract

This paper reports the process of production optimization and partial characterization of xylanase from a newly isolated Bacillus amyloliquefacies VR002, isolated from local soil. The microorganism exhibited maximum xylanase production when 1.0% (v/v) of inoculum size was added to culture medium with initial pH 6, 1.0% (w/v) birchwood xylan, at 35 °C after 48h of incubation. Xylanase production in different carbon sources apart from birchwood xylan and xylose did not show high production levels. Optimum pH for xylanase activity was 6.0. The enzyme was alkali-stable and retained 100% of residual activity over the pH range from 6.0 to 10.0 for 24 h at 25°C. Optimum temperature for enzyme activity was 55°C. Xylanase was 100% stable at 4°C and 25°C even after 24h of incubation, a desirable characteristic for enzyme storage. Moreover, best crude extract volume and time reaction were found to be 10 µL and 5 min, respectively. After optimization of production and activity parameters, an increase of nearly 60-fold in xylanase activity (44.12 ± 4.36 U/mL) was achieved. Characteristics of B. amyloliquefaciens VR002 xylanase are particularly desirable for biotechnological applications

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Author Biographies

Bruno Las-Casas Chaves, Universidade Federal Fluminense

Graduation student of Agribusiness Engineering at Universidade Federal Fluminense. He works on scientific Initiation project, studying the optimization of microbial production of the enzyme xylanase. He have studied Biotechnology at Universidade da Beira Interior (Portugal) during one semester.

Ana Paula Martinazzo, Universidade Federal Fluminense

Bachelor's at Agricultural Engineering from Universidade Estadual do Oeste do Paraná (1995), master's at Agricultural Engineering from Universidade Federal de Viçosa (1997) and doctorate at Agricultural Engineering from Universidade Federal de Viçosa (2006). Has experience in Agricultural Engineering, focusing on Engineering of Processing of Agricultural Products, acting on the following subjects: secagem, plantas medicinais, óleo essencial, grãos and cymbopogon citratus.

Brisabella Coca, Universidade Federal Fluminense

She has graduation at Biological Science by Centro Universitário de Barra Mansa (2005) and specialization at Environmental Science by Centro Universitário de Barra Mansa (2007).  Currently she works at Porto Real’s City Hall.  She has experience with General Biology.

Adriane Nunes de Souza, Universidade Salgado de Oliveira

Graduated in Biological Sciences at the Universidade Estadual do Norte Fluminense Darcy Ribeiro (1997), Graduated in Teacher Training _ Full Degree in Biology at Universidade Salgado de Oliveira (2011), Master in Plant Production at Universidade Estadual do Norte Fluminense Darcy Ribeiro (2000) and PhD in Science from the Center for Nuclear Energy in Agriculture - University of São Paulo (2005) and Post Doctorate in Biosciences and Biotechnology from the State University of Norte Fluminense Darcy Ribeiro (2009). Currently, I work as Full Professor at Salgado de Oliveira University. Member of the NDE of the Nursing course - UNIVERSO and of the Collegiate of the Nutrition course - Universe.She has graduation at Biological Science by Centro Universitário de Barra Mansa (2005) and specialization at Environmental Science by Centro Universitário de Barra Mansa (2007).  Currently she works at Porto Real’s City Hall.  She has experience with General Biology.

Carlos Eduardo Teodoro, Universidade Federal Fluminense

Bachelor’s at Agronomy by Universidade Federal Rural do Rio de Janeiro (1997), master's at Crop Production by Universidade Estadual do Norte Fluminense Darcy Ribeiro (1999) and Ph.D. at Bioscience and Biotechnology by Universidade Estadual do Norte Fluminense Darcy Ribeiro (2004) . Currently he is a professor at Universidade Federal Fluminense and reviewer of Environmental and Agricultural Engineering Brazilian Journal.

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Published

2020-05-11

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How to Cite

Chaves, B. L.-C., Martinazzo, A. P., Coca, B., de Souza, A. N., & Teodoro, C. E. (2020). Optimization of production and partial characterization of xylanase from a newly isolated Bacillus amyloliquefaciens. Ciência E Natura, 42, e9. https://doi.org/10.5902/2179460X42741

Issue

Vol. 42 (2020): 40 YEARS - Anniversary Edition

Section

Chemistry

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