Total Phenolic and Flavonoid Content and Antioxidant Activity of Vitex Montevidensis (Spreng) Moldenke - Verbenaceae

Authors

  • Sideney Becker Onofre Universidade Comunitária da Região de Chapecó - UNOCHAPECÓ - Center of Exact and Environmental Sciences - ACEA - Technology and Innovation Management Posgraduate Program - PPGTI - Av. Senador Attílio Fontana, 591-E EFAPI - 89809-000 - Chapecó - Santa Catarina - Brazil.
  • Dirceu Abatti União de Ensino do Sudoeste do Paraná - UNISEP - Faculdade Educacional de Dois Vizinhos - FAED - Av. Presidente Kennedy, 2601 - 85660-000 - Dois Vizinhos - Paraná - Brazil.
  • Amarildo Antonio Tessaro União de Ensino do Sudoeste do Paraná - UNISEP - Faculdade Educacional de Dois Vizinhos - FAED - Av. Presidente Kennedy, 2601 - 85660-000 - Dois Vizinhos - Paraná - Brazil.
  • Alessandra Buss Tessaro Federal University of Pelotas – UFPel - Postgraduate Program in Materials Science and Engineering. Rua Flores da Cunha, 809 – Pelotas – Rio Grande do Sul – Brazil.

DOI:

https://doi.org/10.5902/2179460X21363

Keywords:

phenol, flavonoids, verbenaceae, secondary metabolites.

Abstract

In recent years, a substantial amount of evidence has pointed to the key role of free radicals and other oxidants as the main culprits for aging and degenerative diseases associated with aging, such as cancer, cardiovascular diseases, cataract, decline of the immune system and brain dysfunctions. The objective of this work was therefore to detect variations in total phenol and flavonoid content, and in antioxidant activity of samples of V. montevidensis. Dried samples were submitted to extraction to obtain the hexane, ethyl acetate and ethanol fractions. Phytochemical prospecting and an analysis of the phenolic content and antioxidant activity was carried out. The data was analyzed according to the mean±standard deviation and submitted to analysis of variance followed by Tukey's test with a significance of (p < 0.05). Flavonoids, tannins, coumarins, terpenoids, steroids, alkaloids and anthraquinones were detected in the samples. The content of total flavonoids varied between 3.18±0,58 and 7.22±0,17 g/100g, while the total phenols ranged from 1.13±0.16 to 18.44±0.21 g/100g. The extracts produced EC50 between 339.75±6.56 and 278.33±23.11 μg/mL.

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Author Biography

Sideney Becker Onofre, Universidade Comunitária da Região de Chapecó - UNOCHAPECÓ - Center of Exact and Environmental Sciences - ACEA - Technology and Innovation Management Posgraduate Program - PPGTI - Av. Senador Attílio Fontana, 591-E EFAPI - 89809-000 - Chapecó - Santa Catarina - Brazil.

Graduação em Ciências Biológicas, Mestre e Doutor em Processos Biotecnológicos pela UFPR. Pós doutor em Impactos Ambientais - UNAM - MEX.

 

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Published

2016-09-28

How to Cite

Onofre, S. B., Abatti, D., Tessaro, A. A., & Tessaro, A. B. (2016). Total Phenolic and Flavonoid Content and Antioxidant Activity of Vitex Montevidensis (Spreng) Moldenke - Verbenaceae. Ciência E Natura, 38(3), 1197–1204. https://doi.org/10.5902/2179460X21363

Issue

Vol. 38 No. 3 (2016)

Section

Biology

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