Cytotoxicity and genotoxicity of aqueous and hydroalcoholic extracts from Gallesia Integrifolia (Spreng) Harms

Authors

DOI:

https://doi.org/10.5902/2179460X84759

Keywords:

Medicinal plant, Cytogenotoxicity, Mutagenicity, Allium cepa, RAPD

Abstract

Gallesia integrifolia (Spreng) Harms is a medicinal plant commonly known in Brazil as pau-d'alho. This large tree species belongs to family Phytolaccaceae, which is widely distributed in several Brazilian states. Studies carried out with extracts deriving from different parts of this plant have evidenced its acaricidal, larvicidal, antifungal and bactericidal properties, among others. Thus, given its potential to be used as therapeutic alternative, the aim of the current study was to assess the cytogenetic and mutagenic effect of G. integrifolia, based on using the Allium cepa system and Random Amplified Polymorphic DNA (RAPD) as analysis tools, as well as on germination assay conducted with Lactuca sativa L. Results have indicated that aqueous and hydroalcoholic extracts from G. integrifolia leaves presented cytotoxic, genotoxic and mutagenic effects at the tested concentrations. The herein observed effects may be associated with phytochemical agents found in the tested extracts, as well as emphasize the importance of raising awareness about the indiscriminate use of medicinal plants. Thus, future research should be conducted to help isolating and better understanding the structure of components capable of inhibiting cell division.

Downloads

Download data is not yet available.

Author Biographies

Sthefany Pereira Duarte, Universidade Federal Rural do Rio de Janeiro

Academic in pharmacy at the Federal Rural University of Rio de Janeiro (UFRRJ)

Alexia Barreto da Silveira, Universidade Federal Rural do Rio de Janeiro

Academic in pharmacy at the Federal Rural University of Rio de Janeiro (UFRRJ)

Caroline de Almeida Azevedo, Universidade Federal Rural do Rio de Janeiro

Pharmacist, graduated in pharmacy at Federal Rural University of Rio de Janeiro (UFRRJ)

Brena Guerra Paixão, Universidade Federal do Rio de Janeiro

Academic in pharmacy at the Federal Rural University of Rio de Janeiro (UFRRJ)

Matheus de Lima Rodrigues, Universidade Federal do Rio de Janeiro

Academic in pharmacy at the Federal Rural University of Rio de Janeiro (UFRRJ)

Saulo Tavares Abreu, Universidade Federal do Rio de Janeiro

Pharmacist, graduated in pharmacy at Federal Rural University of Rio de Janeiro (UFRRJ) and master’s student at the postgraduate program in Pharmacology and Medicinal Chemistry at the Federal University of Rio de Janeiro (UFRJ)

Jennifer Vieira Gomes, Universidade do Estado do Rio de Janeiro

Biologist, graduated in biological sciences from Federal Rural University of Rio de Janeiro (UFRRJ), Master in Biosciences from the State University of Rio de Janeiro (UERJ) and doctoral student in the postgraduate program in Bioscience at the State University of Rio de Janeiro (UERJ)

Helcio Resende Borba, Universidade Federal do Rio de Janeiro

Biologist and Pharmacist, PhD in Veterinary Sciences (UFRRJ) and Full Professor form the Federal Rural University of Rio de Janeiro (UFRRJ)

Viviane Moreira Lima, Universidade Federal do Rio de Janeiro

Biologist, Douctor's in Biological Sciences (genetic) from the Federal University of Rio de Janeiro (UFRJ) and Associate Professor at the Federal Rural University of Rio de Janeiro (UFRRJ).

References

ALBARICI, T. R.; VIEIRA, P. C.; FERNANDES, J. B.; SILVA, M. F. D. G. F. D.; PIRANI, J. R. Cumarinas e alcaloides de Rauia resinosa (Rutaceae). Química Nova, v. 33, p. 2130-2134, 2010.

AKSAKAL, O.; ERTURK, F. A.; SUNAR, S.; BOZARI, S.; AGAR, GULERAY. Assessment of genotoxic effects of 2,4-dichlorophenoxyacetic acid on maize by using RAPD analysis. Industrial Crops and Products, v. 42 (2013), p. 552-557, 2013.

ARUNACHALAM, K.; ASCÊNCIO, S. D.; SOARES, I. M.; SOUZA AGUIRA, R. W.; DA SILVA, L. I.; DE OLIVEIRA, R. G.; BALOGUN, S. O.; DE OLIVEIRA MARTINS, D. T. Gallesia integrifolia (Spreng.) Harms: In vitro and in vivo antibacterial activities and mode of action. Journal of Ethnopharmacology, vol. 184, p 128-137, 2016.

ARUNACHALAM, K.; BAGOLUN, S. O.; PAVAN, E.; ALMEIDA, G. V. B.; OLIVEIRA, R. G.; WAGNER, T.; FILHO, V. C.; MARTINS, D. T. O. Chemical characterization, toxicology and mechanism of gastric antiulcer action of essential oil from Gallesia integrifolia (Spreng.) Harms in the in vitro and in vivo experimental models. Biomedicine & Pharmacotherapy, v. 94, p. 292-306, 2017.

AYRES M.; AYRES J. R.; AYRES D. L.; SANTOS A. A. S. BioEstat 5.0: Aplicações Estatísticas nas Áreas das Ciências Biológicas e Médicas. 5ed. Belém: Sociedade Civil Mamirauá, 2007.

BHATTACHARYA, S.; HALDAR, P. K. Evaluation of antimitotic and genotoxic effects of the triterpenoid enriched extract from Trichosanthes dioica root. America-Eurasian Journal of Toxicology Sciences, v. 4 n.1, p. 20-23, 2012.

BORGES, F.; ROLEIRA, F.; MILHAZES, N.; SANTANA, L.; URIARTE, E. Simple coumarins and analogues in medicinal chemistry: occurrence, synthesis and biological activity. Current Medicinal Chemistry, v. 12 n.8, p. 887-916, 2005.

BORTOLUCCI, W.; OLIVEIRA, H.; OLIVA, L.R.; GONÇALVES, J.E.; JÚNIOR, R.P.; COLAUTO, N.B.; LINDE, G.A.; GAZIM, Z.C. Crude ethanolic extracts of diferente parts of Gallesia integrifolia (Phytolaccaceae) for the control of Rhipicephalus microplus. International Journal of Acarology, 2020.

BORTOLUCCI, W. C.; RAIMUNDO, K. F.; FERNANDEZ, C. M. M.; CALHELHA, R. C.; FERREIRA, I. C. F. R.; BARROS, L.; GONÇALVES, J. E.; LINDE, G. A.; COLAUTO, N. B.; GAZIM, Z. C. Cytotoxicity and anti-inflammatory activities of Gallesia integrifólia (Phytolaccaceae) fruit essential oil. Natural Product Research, v. 36 n.11, p. 2878-2883, 2022.

Cai L. Y.; Shi F. X.; Gao X. Preliminary phytochemical analysis of Acanthopanan trifoliatus (L.) Merr. J Med Plant Res., v. 5 n.17, p. 4059-4064, 2011.

CAPOBIANGO, R. A.; VESTENA, S.; BITTENCOURT, A. H. C. Alelopatia de Joanesia princeps Vell. e Casearia sylvestris Sw. Sobre espécies cultivadas. Revista Brasileira de Farmacognosia, v. 19, p. 924-930, 2009.

CHUNG, K-T.; WEI, C-I.; JOHNSON, M. G. Are tannins a double-edged sword in biology and health? Trends in Food Science & Technology, v. 9, p. 168-175, 1998.

DE-CAMPOS-BORTOLUCCI, W.; MARKO-DE-OLIVEIRA, H. L.; ROQUE-OLIVA, L.; GONÇALVES, J. E.; PIAU-JÚNIOR, R.; MARIANO-FERNANDEZ, C. M.; GAZIM, Z. C. Crude extract of the tropical tree Gallesia integrifolia (Phytolaccaceae) for the control of Aedes aegypti (Diptera: Culicidae) larvae. Revista de Biologia Tropical, v. 69 n. 1, p. 153-169, 2021.

DE CASTRO E SOUSA JM, PERON AP, DA SILVA E SOUSA L, HOLANDA MM, LIMA AMV, DE OLIVEIRA VA, ET AL. Cytotoxicity and genotoxicity of Guaribas river water (Piauí, Brazil), influenced by anthropogenic action. Environ Monit Assess., v.189 n. 6, p. 1-11, 2017.

FENECH, M. The role of folic acid and Vitamin B12 in genomic stability of human cells. Mutation Research, v. 475 n. 1-2, p. 57-67, 2001.

FILIPPIN, C.; FELIPE, L. M. B.; NASCIMENTO, A. J. D.; LEONART, M. S. S. Estudos sobre a variaçäo interobservadores em citologia cérvico-vaginal. Rev. bras. anal. clin, p. 239-42, 2000.

FU, P. P. Pyrrolizidine alkaloids—genotoxicity, metabolism enzymes, metabolic activation, and mechanisms. Drug metabolism reviews, v. 36 n. 1, p. 1-55, 2004.

GERSHENZON, J.; DUDAREVA, N. The function of terpene natural products in the natural world. Nature Chemical Biology, v. 3, p. 408-414, 2007.

HAQ, I.; KUMARI, V.; KUMAR, S.; RAJ, A.; LOHANI, M.; BHARGAVA, R. N. Evaluation of the phytotoxic and genotoxic potential of pulp and paper mill efluente using Vigna radiata and Allium cepa. Advances in Biology, v. 2016, p. 1-11, 2016.

HE, Y.; ZHU, L.; MA, J.; LIN, G. Metabolism-mediated cytotoxicity and genotoxicity of pyrrolizidine alkaloids. Archives of Toxicology, v. 95 n. 6, p. 1917-1942, 2021.

HEDDLE, J. A.; HITE, M.; KIRKHART, B.; MAVOURNIN, K.; MACGREGOR, J. T.; NEWELL, G. W.; SALAMONE, M. F. The induction of micronuclei as a measure of genotoxicity A Report of the U.S. Environmental Protection Agency Gene-Tox Program. Mutation Research, v. 123, p. 61-118, 1983.

HEMACHANDRA, C. K.; PATHIRATNE, A. Combination of physico-chemical analysis, Allium cepa test system and Oreochromis niloticus erythrocyte based comet assay/nuclear abnormalities tests for cyto-genotoxicity assessments of treated effluents discharged from textile industries. Ecotoxicology and environmental safety, v. 131, p. 54-64, 2016

HSIEH, C.Y.J.; SUN, M.; OSBORNE, G.; RICKER, K.; TSAI, F. C.; LI, K.; TOMAR, R.; PHUONG, J.; SCHMITZ, R.; SANDY, M. S. Cancer hazard identification integrating human variability: The case of coumarin. International Journal of Toxicology, v. 38 n. 6, p. 501-552, 2019.

KOÇ, K.; PANDIR, D. All aspect of toxic effect of brilliant blue and sunset yellow in Allium cepa roots. Cytotechnology, v. 70, p. 449-463, 2018.

LACERDA, G. E.; VALADARES, M. B.; GRATÃO, L. H. A.; DE OLIVEIRA, S. R. U.; DO NASCIMENTO, G. N. L. Compostos fenólicos. In: Nascimento, G. N. L.; Pereira, R. J. (Org.). Compostos bioativos vegetais, 1ed. Palmas: EDUFT, 2014, v.1, p.66-81.

LORENZI, H. Árvores brasileiras: Manual de identificação e cultivo de plantas arbóreas nativas do Brasil. 4 ed. São Paulo: Instituto Plantarum de Estudos da Flora LTDA, 2002

MATOS, F. J. A. Introdução à fitoquímica experimental. 3 ed. Fortaleza: Editora UFC, 2009.

MAUGERI, A.; LOMBARDO, G. E.; CIRMI, S.; SÜNTAR, I.; BARRECA, D.; LAGANÀ, G.; NAVARRA, M. Pharmacology and toxicology of tannins. Archives of Toxicology, v. 96, p. 1257-1277, 2022.

MONTAGNER, C. Atividade antifúngica e citotóxica de cumarinas naturais e semi-sintéticas. 2007. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Santa Catarina, Florianópolis, SC, 2007.

MONTANARO L, TRERÉ D, DERENZINI M. Nucleolus, ribosomes, and cancer. The American Journal of Pathology, v. 173 n. 2, p. 301-310, 2008.

MONTEIRO, J. M.; ALBUQUERQUE, U. P. D.; ARAÚJO, E. D. L.; AMORIM, E. L. C. D. Taninos: uma abordagem da química à ecologia. Química Nova, v. 28, p. 892-896, 2005.

MORSY, N. Phytochemical analysis of biologically active constituents of medicinal plants. Main Group Chemistry, v. 13 n. 1, p. 7-21, 2014.

PIZZI, A. Tannins: Prospectives and actual industrial applications. Biomolecules, v. 9 n. 8, p. 1-30, 2019.

RAIMUNDO, K. F., W. DE C. BORTOLUCCI, J. GLAMOČLIJA, M. SOKOVIĆ, J. E. GONÇALVES, G. A. LINDE, N. B. COLAUTO, AND Z. C. GAZIM. Antifungal activity of Gallesia integrifolia fruit essential oil. Braz. J.Microbiol., v. 49, p. 229–235, 2018.

RODRIGUES, K. A. F.; DIAS, C. N.; FLORÊNCIO, J. C.; VILANOVA, C. M.; GONÇALVES, J. R. S.; COUTINHO-MORAES, D. F. Prospecção fitoquímica e atividade moluscicida de folhas de Momordica charantia L. Caderno de Pesquisa, v. 17, p. 69-77, 2010.

RUJJANAWATE, C.; KANJANAPOTHI, D.; PANTHONG, A. Pharmacological effect and toxicity of alkaloids from Gelsemium elegans Benth. Journal of Ethnopharmacology, v. 89, p. 91-95, 2003.

SAKLANI S.; MISHRA A. P.; SATI B.; SATI H. Pharmacognostic, phytochemical and antimicrobial, screening of Polystachya, an endangered medicinal tree. Int J Pharm Pharm Sci., v. 4 n.3, p. 235-240, 2012.

SHAHWAR, D.; ANSARI, M. Y. K.; PARK, Y. Physio-biochemical analysis and molecular characterization of induced lentil mutante lines. PLOS ONE, v. 17 n. 10, p 1-24, 2022.

SILVA, F. C. O.; FERREIRA, M. K. A.; SILVA, A. W.; MATOS, M. G. C.; MAGALHÃES, F. E. A.; SILVA, P. T.; BANDEIRA, P. N.; DE MENEZES, J. E. S. A.; SANTOS, H. S. Bioatividades de triterpernos isolados de plantas: Uma breve revisão. Revista Virtual de Química, v. 12 n. 1, p. 1-14, 2020.

SOUZA, A. N. V.; FARIA, M. G. I.; ROCHA, C. E.; PHILIPPSEN, G. S.; SILVA, G. C. C.; SILVA, G. R.; INUMARO, R. S.; GAZIM, J. E. G.; CRISTIANI. Z.; WIETZIKOSKI, S.; LÍVERO, F. A. R.; SEIXAS, F. A. V.; LOVATO, E. C. W. Bioactive compounds with antifungal activity against pathogens isolated from pregnant woman: Gallesia integrifolia (garlic wood) is a promising treatment for vulvovaginal candidiasis. Journal of Ethnopharmacology, v. 295 n. 115403, 2022.

STANGE V. S.; GOMES T. D. U. H.; DE ANDRADE M. A.; BATITUCCI M. C. P. Avaliação do efeito mutagênico do hydroalcoholic extract bruto, por meio de bioensaios in vivo e prospecção fitoquímica de Cecropia glazioviii Sneth (embaúba), Cecropiaceae. Bras J Pharmacogn., v. 19 n. 2B, p. 637-642, 2009.

SWAILEH, K. M.; BARAKAT, S. O.; HUSSEIN, R. M. RAPD Assessment of in vivo induced genotoxicity of raw and treated wastewater to albino rat. Bull Environ Contam Toxicol, v. 90, p. 621-625, 2013.

TEDESCHI, P.; BONETTI, G.; MAIETTI, A.; BRANDOLINI, V. Random amplified polymorphic DNA (RAPD) fingerprint and antioxidants profile as markers for Tropea red onion (Allium cepa L.) authenticity. Journal of Food Composition and Analysis, v. 36, p. 98-103, 2014.

TREASE G. E.; EVANS W. C. Pharmacognosy. 16. ed. London: Scandars Company Ltd, 1989.

WIJEYARATNE, W. M. D. N.; WICKRAMASINGHE, M. U. Chromosomal abnormalities in Allium cepa induced by treated textile effluents: spatial and temporal variations. Journal of Toxicology, v. 2020, p 1-10, 2020.

YORDI, E. G.; MATOS, M. J.; MARTÍNEZ, A. P.; TORNES, A. C.; SANTANA, L.; MOLINA, E.; URIARTE, E. In sílico genotoxicity of courmarins: application of the phenol-explorer food database to funcional food science. Food & Function, v. 8, p. 2958-2966, 2017.

Downloads

Published

2024-07-29

How to Cite

Duarte, S. P., Silveira, A. B. da, Azevedo, C. de A., Paixão, B. G., Rodrigues, M. de L. ., Abreu, S. T., Gomes, J. V., Borba, H. R., & Lima, V. M. (2024). Cytotoxicity and genotoxicity of aqueous and hydroalcoholic extracts from Gallesia Integrifolia (Spreng) Harms. Ciência E Natura, 46, e84759. https://doi.org/10.5902/2179460X84759

Issue

Vol. 46 (2024): Publicação contínua

Section

Biology-Genetics

License

Copyright (c) 2024 Ciência e Natura

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

To access the DECLARATION AND TRANSFER OF COPYRIGHT AUTHOR’S DECLARATION AND COPYRIGHT LICENSE click here.

 

Ethical Guidelines for Journal Publication

The Ciência e Natura journal is committed to ensuring ethics in publication and quality of articles. 

Conformance to standards of ethical behavior is therefore expected of all parties involved: Authors, Editors, Reviewers, and the Publisher.

In particular, 

Authors: Authors should present an objective discussion of the significance of research work as well as sufficient detail and references to permit others to replicate the experiments. Fraudulent or knowingly inaccurate statements constitute unethical behavior and are unacceptable. Review Articles should also be objective, comprehensive, and accurate accounts of the state of the art. The Authors should ensure that their work is entirely original works, and if the work and/or words of others have been used, this has been appropriately acknowledged. Plagiarism in all its forms constitutes unethical publishing behavior and is unacceptable. Submitting the same manuscript to more than one journal concurrently constitutes unethical publishing behavior and is unacceptable. Authors should not submit articles describing essentially the same research to more than one journal. The corresponding Author should ensure that there is a full consensus of all Co-authors in approving the final version of the paper and its submission for publication.

Editors: Editors should evaluate manuscripts exclusively on the basis of their academic merit. An Editor must not use unpublished information in the editor's own research without the express written consent of the Author. Editors should take reasonable responsive measures when ethical complaints have been presented concerning a submitted manuscript or published paper.

Reviewers: Any manuscripts received for review must be treated as confidential documents. Privileged information or ideas obtained through peer review must be kept confidential and not used for personal advantage. Reviewers should be conducted objectively, and observations should be formulated clearly with supporting arguments, so that Authors can use them for improving the paper. Any selected Reviewer who feels unqualified to review the research reported in a manuscript or knows that its prompt review will be impossible should notify the Editor and excuse himself from the review process. Reviewers should not consider manuscripts in which they have conflicts of interest resulting from competitive, collaborative, or other relationships or connections with any of the authors, companies, or institutions connected to the papers.

Most read articles by the same author(s)