In vitro antioxidant and anticholinesterase screening of five plant species collected from the Discovery Coast, Bahia, Brazil

Authors

DOI:

https://doi.org/10.5902/2179460X73958

Keywords:

Southern Bahia, Cholinesterase Activity, Atlantic Rainforest, Biological Research

Abstract

Although most of the species Dysphania ambrosioides (L.) Mosyakin & Clemants, Lippia alba (Mill.) N.E.Br. ex Britton & P.Wilson, Rosmarinus officinalis L., Schinus terebinthifolia Raddi, and Tragia volubilis L. are not native to Brazil, they have been adapted and widely-used in folk medicine and spiritual rituals in the Discovery Coast region of Bahia. Within this context, considering the need for knowledge of the pharmacological properties of plants and awareness of the influence of environmental factors, this study was conducted. This study therefore investigated the phenolic composition, antioxidant, and anticholinesterase (Anti-AChE) properties of plant extracts collected from the area known as the Discovery Coast, in Porto Seguro, Bahia, Brazil. In addition, this study presented data on biological investigations described in the literature in order to contribute to scientific knowledge and gather evidence that validates the plants used in traditional medicine. Anti-AChE was strongly indicated in the extracts with higher antioxidant activity. Also, the results suggest that the higher phenolic content of the extracts may be responsible for their antioxidant and Anti-AChE activity. Although many results from the biological activities in the literature converge on some popular applications for the plants, further studies are still required to corroborate their therapeutic effectiveness as well as provide technical clarifications regarding their use, preparation, and purpose.

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Author Biographies

Luciano da Silva Lima, Instituto Federal da Bahia

PhD in Chemistry (Organic Chemistry) from the Federal University of Bahia. He has a degree in Chemistry from the State University of Santa Cruz (2003) and a master's degree in Chemistry from the Federal University of Bahia (2005). He has experience in Chemistry, with an emphasis on Natural Products Chemistry, biotransformation, spectrometric techniques (MS, IR, UV, NMR), GC and HPLC-MS-DAD. He is currently a professor at the Federal Institute of Bahia, Porto Seguro campus.

Géssica Carvalho Pereira, Secretaria da Educação do Estado da Bahia

Teacher at Colégio Modelo Luís Eduardo Magalhaes, Graduated in Chemistry

Hênia Joelia Magalhâes Cruz, Instituto Federal da Bahia

Master's degree in Chemistry (Organic Chemistry) from the Federal University of Bahia (2013). She has a Bachelor's degree in Chemistry from the Federal University of Bahia (2010). She is currently a substitute teacher at the Federal Institute of Bahia, Porto Seguro campus, Porto Seguro and carries out research activities with the Food Research Team: Technology and Chemistry - EPATEQ.

André Búrigo Leite, Instituto Federal da Bahia

PhD in Teaching, Philosophy and History of Science from the Federal University of Bahia/State University of Feira de Santana (UFBA-UEFS, 2019). Professor in technical, undergraduate and specialization courses at the Federal Institute of Education, Science and Technology of Bahia - IFBA - Campus Porto Seguro, BA. Experience in Chemical Engineering, with an emphasis on Environmental Engineering and Teaching, working mainly on the following topics: numerical simulation, gas absorption process, water desorption process, air pollution, genotoxicity, biofuels, solid waste, biodigesters, composting and environmental education. Evaluator of undergraduate courses by INEP.

Jorge Antônio Silva Costa, Universidade Federal do Sul da Bahia

Professor in Graduate Program in Environmental Technology Science, Federal University of Southern Bahia. PhD in Botanic.

Marcus Luciano Souza de Ferreira Bandeira, Instituto Federal da Bahia

Professor in Graduate Program in Environmental Technology Science, Instituto Federal da Bahia. PhD in Chemistry

References

Agra, M. D. F., Freitas, P. F. D., & Barbosa-Filho, J. M. (2007). Synopsis of the plants known asmedicinal and poisonous in Northeast of Brazil. Revista Brasileira de Farmacognosia, 17, 114-140.https://doi.org/10.1590/S0102-695X2007000100021

Aktumsek, A., Zengin, G., Guler, G. O., Cakmak, Y. S., & Duran, A. (2013). Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food and chemical toxicology, 55, 290-296. doi.org/10.1016/j.fct.2013.01.018

Alves, C. Q., David, J. M., David, J. P., Bahia, M. V., & Aguiar, R. M. (2010). Métodos para determinação de atividade antioxidante in vitro em substratos orgânicos. Química Nova, 33, 2202-2210. https://doi.org/10.1590/S0100-40422010001000033

Alves, C. Q., Lima, L. S., David, J. M., Lima, M. V., David, J. P., Lima, F. W., Pedroza, K.C.M.C, & Queiroz, L. P. (2013). In vitro acetylcholinesterase activity of peptide derivatives isolated from two species of Leguminosae. Pharmaceutical Biology, 51(7), 936-939. https://doi.org/10.3109/13880209.2013.770536

Arantes, S., Piçarra, A., Candeias, F., Teixeira, D., Caldeira, A. T., & Martins, M. R. (2017). Antioxidant activity and cholinesterase inhibition studies of four flavouring herbs from Alentejo. Natural product research, 31(18), 2183-2187.https://doi.org/10.1080/14786419.2017.1278598

Bourhia, M., Laasri, F. E., Aourik, H., Boukhris, A., Ullah, R., Bari, A., Benbacer, L., & Gmouh, S. (2019). Antioxidant and antiproliferative activities of bioactive compounds contained in Rosmarinus officinalis used in the Mediterranean diet. Evidence-based complementary and alternative medicine, 2019. https://doi.org/10.1155/2019/7623830

Brasil. Ministério do Meio Ambiente. (2020, 16 mar). Biodiversidade Brasileira. https://www.gov.br/mma/pt-br/assuntos/ecossistemas-1.

Brasil. (2020, 3 aug.) Política Nacional de Plantas Medicinais e Fitoterápicos. https://www.planalto.gov.br/ccivil_03/_Ato2004-2006/2006/Decreto/D5813.htm. Calixto, J. B. (2000). Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Brazilian Journal of medical and Biological research, 33, 179-189. https://doi.org/10.1590/S0100-879X2000000200004

Chase, M. W., Christenhusz, M. J, Fay, M. F., Byng, J. W., Judd Soltis, D. E., Mabberley, D. J., Sennikov, A. N., Soltis, P. S. (2016). An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG IV. Botanical journal of the Linnean Society, 181(1), 1-20. https://doi.org/10.1111/boj.12385

Dolghi, A., Coricovac, D., Dinu, S., Pinzaru, I., Dehelean, C. A., Grosu, C., Merghes, P. E., & Sarau, C. A. (2022). Chemical and antimicrobial characterization of Mentha piperita L. and Rosmarinus officinalis L. essential oils and in vitro potential cytotoxic effect in human colorectal carcinoma cells. Molecules, 27(18), 6106. https://doi.org/10.3390/molecules27186106

Eid, A. M., Jaradat, N., Issa, L., Abu-Hasan, A., Salah, N., Dalal, M., Mousa, A., & Zarour, A. (2022). Evaluation of anticancer, antimicrobial, and antioxidant activities of rosemary (Rosmarinus Officinalis) essential oil and its Nanoemulgel. European Journal of Integrative Medicine, 55,102175. https://doi.org/10.1016/j.eujim.2022.102175

Ellman, G. L., Courtney, K. D., Andres Jr, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88-95. . https://doi.org/10.1016/0006-2952(61)90145-9

Forlenza, O. V. (2005). Tratamento farmacológico da doença de Alzheimer. Archives of Clinical Psychiatry, São Paulo, 32, 137-148. https://doi.org/10.1590/S0101-60832005000300006

Furlan, C. M., Santos, K. P., Sedano-Partida, M. D., Motta, L. B. D., Santos, D. Y. A., Salatino, M. L. F., Negri, G., Berry, P. E., Van Ee, B. W., & Salatino, A. (2015). Flavonoids and antioxidant potential of nine Argentinian species of Croton (Euphorbiaceae). Brazilian Journal of Botany, 38, 693-702. https://doi.org/10.1007/s40415-014-0115-9

Ghavam, M. (2022). GC-MS analysis and antimicrobial activities of a Rosmarinus officinalis L. essential oil from Kashan Region (Iran). Biochemical Systematics and Ecology, 105, 104507. https://doi.org/10.1016/j.bse.2022.104507

Ghribia, L., Ghouilaa, H., Omrib, A., Besbesb, M., & Janneta, H. B. (2014). Antioxidant and anti–acetylcholinesterase activities of extracts and secondary metabolites from Acacia cyanophylla. Asian Pacific Journal of Tropical Biomedicine, , 4, S417-S423. https://doi.org/10.12980/APJTB.4.2014C1038

Gobbo-Neto, L., & Lopes, N. P. (2007). Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários. Química Nova, 30, 374-381. https://doi.org/10.1590/S0100-40422007000200026

Haida, S., & Kribii, A. (2020) Chemical composition, phenolic content and antioxidant capacity of Haloxylon scoparium extracts. South African Journal of Botany, 131, 151-160. https://doi.org/10.1016/j.sajb.2020.01.037

Heinrich, M., Kufer, J., Leonti, M., & Santayana, M. P. (2006). Ethnobotany and ethnopharmacology – Interdisciplinary links with the historical sciences. Journal of ethnopharmacology, 107(2), 157-160. https://doi.org/10.1016/j.jep.2006.05.035.

Hlila, M. B., Mosbah, H., Mssada, K., Jannet, H. B., Aouni, M., & Selmi, B. (2015). Acetylcholinesterase inhibitory and antioxidant properties of roots extracts from the Tunisian Scabiosa arenaria Forssk. Industrial Crops and Products, , 67, 62-69. https://doi.org/10.1016/j.indcrop.2015.01.009

Iwanaga, C. C., Ferreira, L. D. A. O., Bernuci, K. Z., Fernandez, C. M. M., Lorenzetti, F. B., Sehaber, C. C., Frez, F. C. V., Bernardes, S. S., Panizzon, G. P., Linde, G. A., Vieira, M. Do C., Zanoni, J. N., & Cortez, D. A. G. (2019). In vitro antioxidant potential and in vivo effects of

Schinus terebinthifolia Raddi leaf extract in diabetic rats and determination of chemical composition by HPLC-ESI-MS/MS. Natural product research, , 33(11), 1655-1658. https://doi.org/10.1080/14786419.2018.1425848

Kakouri, E., Nikola, O., Kanakis, C., Hatziagapiou, K., Lambrou, G. I., Trigas, P., Kanaka-Gantenbein, C., & Tarantilis, P. A. (2022). Cytotoxic Effect of Rosmarinus officinalis Extract on Glioblastoma and Rhabdomyosarcoma Cell Lines. Molecules, 27(19), 6348. https://doi.org/10.3390/molecules27196348

Kamli, M. R., Sharaf, A. A. M., Sabir, J. S., Rather, I. A. (2022). Phytochemical Screening of Rosmarinus officinalis L. as a Potential Anticholinesterase and Antioxidant–Medicinal Plant for Cognitive Decline Disorders. Plants,11(4), 514. https://doi.org/10.3390/plants11040514

Kandsi, F., Elbouzidi, A., Lafdil, F. Z., Meskali, N., Azghar, A., Addi, M., Hano, C., Maleb, A., & Gseyra, N. (2022). Antibacterial and Antioxidant Activity of Dysphania ambrosioides (L.) Mosyakin and Clemants Essential Oils: Experimental and Computational Approaches. Antibiotics, 11(4), 482. https://doi.org/10.3390/antibiotics11040482

Kasali, F. M., Kadima, J. M. N., Tusiimire, J., Ajayi, C. O., & Agaba, A. G. (2022). Effects of the Oral Administration of Aqueous and Methanolic Leaf Extracts of Chenopodium ambrosioides L. (Amaranthaceae) on Blood Glucose Levels in Wistar Rats. Journal of Experimental Pharmacology, 139-148. https://orcid.org/0000-0003-1411-6517

Kumar, N., & Goel, N. (2019). Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnology Reports,24, e00370. https://doi.org/10.1016/j.btre.2019.e00370

Kumar, N., Gupta, S., Chand Yadav, T., Pruthi, V., Kumar Varadwaj, P., & Goel, N. (2019). Extrapolation of phenolic compounds as multi-target agents against cancer and inflammation. Journal of Biomolecular Structure and Dynamics, [2355-2369. https://doi.org/10.1080/07391102.2018.1481457

Leite, P. M., Miranda, A. P., Gomes, I., Rodrigues, M. L., Camargos, L. M., Amorim, J. M., Duarte, R. C. F., Faraco, A. A. G., Carvalho, M. G., & Castilho, R. O. (2023). Antithrombotic potential of Lippia alba: A mechanistic approach. Journal of Ethnopharmacology, 115744. https://doi.org/10.1016/j.jep.2022.115744

Li, J., Si, C., Hong, W., Xia, C., Yang, Y., He, Y., Su, M., Long, X., & Zhang, H. (2022). Identification of the chemical components of ethanol extract of Chenopodium ambrosioides and evaluation of their in vitro antioxidant and anti tumor activities. Tropical Journal of

Pharmaceutical Research,, 21(8), 1689-1697. DOI: 10.4314/tjpr.v21i8.16

Lima, L. S., Rossi, A. S., Cruz, H. J. M., & Bandeira, M. L. S. F., L. D. N. (2020). Ensaio miniaturizado para a quantificação simultânea de antocianinas em flores de Hibiscus. Agropecuária Científica no Semiárido,, 16(2), 102-106. http://dx.doi.org/10.30969/acsa.v16i2.1241

Luz, C. L. S., Mitchell, J. D., & Pell, S. K. (2020). Anacardiaceae in Flora do Brasil 2020 em construção. Jardim Botânico do Rio de Janeiro. Retrieved from: http://floradobrasil.jbrj.gov.br/reflora/floradobrasil/FB1199.

Majolo, C., Bizzo, H. R., Rodrigues Brandão, F., Da Silva, A. M. S., Campos Chagas, E., Chaves, F. C. M., & Muniz, A. W. (2022). Chemical composition of Lippia Lin( (Verbenaceae) essential oils and their antibacterial potential against Aeromonas spp. isolates from Colossoma macropomum. Journal of Essential Oil Research, [s.l.], 34, 1-11.

Mathew, M., & Subramanian, S. (2014). In Vitro Screening for Anti-Cholinesterase and Antioxidant Activity of Methanolic Extracts of Ayurvedic Medicinal Plants Used for Cognitive Disorders. Plos One, 34(3), 251-261. https://doi.org/10.1080/10412905.2022.2036642

Murtiastutik, D., Hidayati, A. N., Widyantari, S., Saputri, B. Y. A., Bintanjoyo, L., Ervianti, E., & Sari, M. (2022). Evaluation of the Antifungal effects of Rosemary Oil and comparison with Nystatin on the Growth of Candida species isolates from HIV/AIDS patients with Oral Candidiasis. Research Journal of Pharmacy and Technology, 15(8), 3755-3758. DOI:10.52711/0974-360X.2022.00630

Miguel, M. G., Nunes, S., Dandlen, S. A., Cavaco, A. M., & Antunes, M. D. (2014). Phenols, flavonoids and antioxidant activity of aqueous and methanolic extracts of propolis (Apis mellifera L.) from Algarve, South Portugal. Food Science and Technology, 34, 16-23. https://doi.org/10.1590/S0101-20612014000100002

Nimse, S. B., & Pal, D. (2015). Free radicals, natural antioxidants, and their reaction mechanisms. RSC advances,5(35), 27986-28006. DOI: 10.1039/C4RA13315C.

Nocchi, S. R., Companhoni, M. V. P., De Mello, J. C. P., Dias Filho, B. P., Nakamura, C. V., Carollo, C. A., Silva, D. B., & Ueda-Nakamura, T. (2017). Antiviral activity of crude hydroethanolic extract from Schinus terebinthifolia against herpes simplex virus type 1. Planta medica, 234(06), 509-518. DOI: 10.1055/s-0042-117774

Ogunleye, Gbenga S., Fagbohun, Oladapo F., Babalola, & Olubunmi O. (2020). Chenopodium ambrosioides var. ambrosioides leaf extracts possess regenerative and ameliorative effects against mercury-induced hepatotoxicity and nephrotoxicity. Industrial Crops and Products, 154, 112723. https://doi.org/10.1016/j.indcrop.2020.112723

Olajide, O. A., Awe, S. O., & Makinde, J. M. (1997). Pharmacological screening of the methanolic extract of Chenopodium ambrosioides. Fitoterapia, 68(6), 529-532. https://pure.hud.ac.uk/en/publications/pharmacological-screening-of-the-methanolic extractof-chenopodiu

Olinto, S. C. F., Calil-Silveira, J., Dias, R., Valle, M. M., Serrano-Nascimento, C., Rocha, P. S., Monteiro-Alfredo, T., Dos Santos, E. L., Souza, K. De P., & Nunes, M. T. (2020). Antiproliferative and Cytotoxic Effects of Schinus terebinthifolia Leaf Extract on Thyroid Follicular Cells. Revista Brasileira de Farmacognosia, 30(5), 693-700. https://doi.org/10.1007/s43450-020-00098-2

Onofre, S. B., Abatti, D., Tessaro, A. A., & Tessaro, A. B. (2016). Total phenolic, flavonoid content and antioxidant activity of Vitex megapotamica (Spreng.) Moldenke. Ciência e Natura, Santa Maria, 38(3), 1197-1204. DOI:10.5902/2179460X21363.

Palma-Duran, S. A., Vlassopoulos, A., Lean, M., Govan, L., & Combet, E. (2017). Nutritional intervention and impact of polyphenol on glycohemoglobin (HbA1c) in non-diabetic and type 2 diabetic subjects: Systematic review and meta-analysis. Critical reviews in food science and nutrition, 57(5), 975-986. https://doi.org/10.1080/10408398.2014.973932

Pascual, M. E., Slowing, K., Carretero, M. E., & Villar, Á. (2001). Antiulcerogenic activity of Lippia alba (Mill.) NE Brown (Verbenaceae). Il Farmaco,56(5-7), 501-504.https://doi.org/10.1016/S0014-827X(01)01086-2.

Pereira, W. S., Da Silva, G. P., Vigliano, M. V., Leal, N. R., Pinto, F. A., Fernandes, D. C., Santos, S. V. M., Martino, T., Nascimento, J. R., De Azevedo, A. P.S., Fonseca, E. N., Velozo, L. S. M., Neto,L. R. S., Bastos, F. F., Portari,E. A., Sabino, K. C. C., Nascimento, F., & Coelho, M. G. (2018). Anti-arthritic properties of crude extract from Chenopodium ambrosioides L. leaves. Journal of Pharmacy and Pharmacology, , 70(8), 1078-1091. https://doi.org/10.1111/jphp.1292.

Pinto, A. C., Silva, D. H. S., Bolzani, V. D. S., Lopes, N. P., & Epifanio, R. D. A. (2002). Produtos naturais: atualidade, desafios e perspectivas. Química nova, 25, 45-61. https://doi.org/10.1590/S0100-40422002000800009

Ramassamy, C. (2006). Emerging role of polyphenolic compounds in the treatment of neurodegenerative diseases: a review of their intracellular targets. European journal of pharmacology, 545(1), 51-64. https://doi.org/10.1016/j.ejphar.2006.06.025

Ramírez, J. J. Q., Rugeles, C. I. G., Stashenko, E. E., Hernández, J. C. M., Galvis, M. L. D., & Sánchez, L. T. G. (2021). In vivo protection against chagasic cardiomyopathy progression using trypanocidal fractions from Lippia alba (Verbenaceae) essential oils. Industrial Crops and Products, , 167, 113553. https://doi.org/10.1016/j.indcrop.2021.113553

Ramos, D. D. B. M., Araújo, M. T. D. M. F., De Lima Araújo, T. C., Dos Santos Neto, O. G., E Silva, M. G., Silva, Y. A., Torre, D. J. L., Patriota, L. L. De P., De Melo, C. M. L., De Lorena, V. M. B., Paiva, P. M. G., Mendes, R. L., & Napoleão, T.H. (2019). Evaluation of antitumor activity and toxicity of Schinus terebinthifolia leaf extract and lectin (SteLL) in sarcoma 180-bearing mice. Journal of ethnopharmacology,233, 148-157. https://doi.org/10.1016/j.jep.2019.01.011

Ramos, D. D. B. M., Araújo, M. T. D. M. F., De Lima Araújo, T. C., Silva, Y. A., Dos Santos, A. C. L. A., E Silva, M. G., Paiva, P. M. G., & Mendes, R. L., (2020). Napoleão, T. H. Antinociceptive activity of Schinus terebinthifolia leaf lectin (SteLL) in sarcoma 180-bearing mice. Journal of ethnopharmacology, 259, 112952. https://doi.org/10.1016/j.jep.2020.112952

Reynertson, K. A., Basile, M. J., & Kennelly, E. J. (2005). Antioxidant potential of seven myrtaceous fruits, Ethnobotany Research and Applications, 3, 025-035. http://hdl.handle.net/10125/151

Sales, G., Medeiros, S., Soares, I., Sampaio, T., Bandeira, M., Nogueira, N., & Queiroz, M. (2022). Antifungal and Modulatory Activity of Lemon Balm (Lippia alba (MILL.) NE BROWN) Essential Oil. Scientia Pharmaceutica, , 90(2), 31. https://doi.org/10.3390/scipharm90020031.

Sampaio, B. L., & Da Costa, F. B. (2018). Influence of abiotic environmental factors on the main constituents of the volatile oils of Tithonia diversifolia. Revista Brasileira de Farmacognosia, 28, 135-144. https://doi.org/10.1016/j.bjp.2018.02.005

Senna, L. (2020). Dysphania in Lista de Espécies da Flora do Brasil. Jardim Botânico do Rio de Janeiro. Retrieved from:http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB139759.

Sharma, Y., Fagan, J., & Schaefer, J. (2020). In vitro Screening for Acetylcholinesterase Inhibition and Antioxidant Potential in Different Extracts of Sage (Salvia officinalis L.) and Rosemary (Rosmarinus officinalis L.). Journal of Biologically Active Products from Nature, 10(1), 59-69. https://doi.org/10.1080/22311866.2020.1729239

Silva, N. M., Lima, L. S., Bandeira, M. L. S. F., Costa, J. A. S., & Leite, A. B. (2022). Evaluation of the antioxidant and photoprotective potential of plants from the Atlantic Forest of southern Bahia. Ciência e Natura, Santa Maria, 44, e60.

Sobrinho, A. C. N., De Morais, S. M., Marinho, M. M., De Souza, N. V., & Lima, D. M. (2021). Antiviral activity on the Zika virus and larvicidal activity on the Aedes spp. of Lippia alba essential oil and β-caryophyllene. Industrial Crops and Products, 162, 113281.

https://doi.org/10.1016/j.indcrop.2021.113281

Soleas, G. J., Grass, L., Josephy, P. D., Goldberg, D. M., & Diamandis, E. P. (2002). A comparison of the anticarcinogenic properties of four red wine polyphenols. Clinical biochemistry, 35(2), 119-124. https://doi.org/10.1016/S0009-9120(02)00275-8.

Tabet, N. (2006). Acetylcholinesterase inhibitors for Alzheimer’s disease: anti-inflammatories in acetylcholine clothing!. Age and ageing, 35(4), 336-338. https://doi.org/10.1093/ageing/afl027

Tang, H., Porras, G., Brown, M. M., Chassagne, F., Lyles, J. T., Bacsa, J., Horswill, A. R., & Quave, C. (2020). L Triterpenoid acids isolated from Schinus terebinthifolia fruits reduce Staphylococcus aureus virulence and abate dermonecrosis. Scientific reports, 10(1), 8046. https://doi.org/10.1038/s41598-020-65080-3

Tlili, N., & Sarikurkcu, C. (2020). Bioactive compounds profile, enzyme inhibitory and antioxidant activities of water extracts from five selected medicinal plants. Industrial crops and products, 151, 112448. https://doi.org/10.1016/j.indcrop.2020.112448

Topal, M., Gulcin, İ. (2022). Evaluation of the in vitro antioxidant, antidiabetic and anticholinergic properties of rosmarinic acid from rosemary (Rosmarinus officinalis L.). Biocatalysis and Agricultural Biotechnology, 43, 102417. https://doi.org/10.1016/j.bcab.2022.102417

Trivellatograssi, L., Malheiros, A., Meyre-Silva, C., Da Silva Buss, Z., Monguilhott, E. D., Fröde, T. S., Da Silva, K. A. B. S., & De Souza, M. M. (2013). From popular use to pharmacological validation: a study of the anti-inflammatory, anti-nociceptive and healing effects of Chenopodium ambrosioides extract. Journal of Ethnopharmacology, 145(1), 127-138. https://doi.org/10.1016/j.jep.2012.10.040

Uliana, M. P., Fronza, M., Da Silva, A. G., Vargas, T. S., De Andrade, T. U., & Scherer, R. (2016). Composition and biological activity of Brazilian rose pepper (Schinus terebinthifolius Raddi) leaves.Industrial Crops and Products, 83, 235-240. https://doi.org/10.1016/j.indcrop.2015.11.077

Zohra, T., Ovais, M., Khalil, A. T., Qasim, M., Ayaz, M., & Shinwari, Z. K. (2019). Extraction optimization, total phenolic, flavonoid contents, HPLC-DAD analysis and diverse pharmacological evaluations of Dysphania ambrosioides (L.) Mosyakin & Clemants.Natural product research, 33(1), 136-142. https://doi.org/10.1080/14786419.2018.1437428.

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2024-11-29

How to Cite

Lima, L. da S., Pereira, G. C., Cruz, H. J. M., Leite, A. B., Costa, J. A. S., & Bandeira, M. L. S. de F. (2024). In vitro antioxidant and anticholinesterase screening of five plant species collected from the Discovery Coast, Bahia, Brazil . Ciência E Natura, 46, e73958. https://doi.org/10.5902/2179460X73958

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