Phenolic compounds, antimicrobial activity, cytotoxicity, and identification of phytochemicals present in Inga marginata Willd seeds

Authors

DOI:

https://doi.org/10.5902/2179460X65941

Keywords:

Total phenolics, Extraction, Identification by ESI-TOF MS, Toxicity, Antimicrobial activity.

Abstract

Brazil has an invaluable source of plants and fruits rich in phenolic compounds important to health, some of which have yet to be discovered. Inga marginata Willd is a fruit that can be found throughout Brazil, and its seeds are rich in phenolic compounds and antimicrobial activity, thus making their extraction and characterization highly relevant. This study aimed to determine the total phenolic compounds, antioxidant capacity, antibacterial and antifungal activities, cytotoxicity evaluation, and characterize the phytochemical compounds present in the extract. The results indicated that extraction by agitation at 30 ºC obtained the highest yield of total phenolic compounds (48.23 mg GAE g-1), antioxidant capacity (40.34 mg TEAC g-1), and IC50 (2.60 mg mL-1). The extracts at 30 and 60 ºC inhibited all microorganisms tested, and the temperature of 30 ºC acted as a bactericide and fungicide at low concentrations. Ten phytochemical compounds were found, mainly being antioxidants and antimicrobials. The cytotoxicity assays showed that Inga marginata seeds do not present cellular cytotoxicity up to the concentration of 250 µg mL-1, maintaining cell viability above 90%.

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Author Biographies

Déborah Cristina Barcelos Flores, Universidade Federal de Santa Maria, Santa Maria, RS

 

Graduated in Food Technology from the Federal University of Santa Maria - UFSM (2015). Graduation in the Special Graduation Program for Teacher Education for Professional Education at the Federal University of Santa Maria - UFSM (2018). Food Technician at the Polytechnic College of UFSM (2018). Master in Food Science and Technology by the Federal University of Santa Maria- UFSM (2020). She is currently a PhD student in the Graduate Program in Food Science and Technology at the Federal University of Santa Maria-UFSM. It conducts research in the area of Food and Health, with a focus on extractions and identification of bioactive compounds from small fruits and use of by-products. Department of Technology and Food Science, Center for Rural Sciences, Federal University of Santa Maria (UFSM), Av. Roraima No. 1000, building 42, Camobi, Zip code 97105-900, Santa Maria, RS, Brazil.


Departamento de Tecnologia e Ciência dos Alimentos, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Av. Roraima nº 1000, prédio 42, Camobi, CEP 97105-900, Santa Maria, RS, Brasil.

 

Caroline Pagnossim Boeira, Universidade Federal de Santa Maria, Santa Maria, RS

Master in Food Science and Technology, graduated in Food Technology from the Federal University of Santa Maria (UFSM). Specialist in Food Science with emphasis on fruits and vegetables from the Federal University of Pelotas (UFPel). She is currently a doctoral student in the Graduate Program in Food Science and Technology (UFSM). It conducts research in the area of food science and technology, with emphasis on meat products, natural antioxidants, extraction of bioactive compounds, use of by-products and bioprocesses. Department of Technology and Food Science, Center for Rural Sciences, Federal University of Santa Maria (UFSM), Av. Roraima No. 1000, building 42, Camobi, Zip code 97105-900, Santa Maria, RS, Brazil.

Departamento de Tecnologia e Ciência dos Alimentos, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Av. Roraima nº 1000, prédio 42, Camobi, CEP 97105-900, Santa Maria, RS, Brasil.

Clarice Madalena Bueno Rolim, Federal University of Santa Maria (UFSM)

Graduated in Pharmacy and Master in Pharmaceutical Science and Technology from the Federal University of Santa Maria, PhD from the University of São Paulo. He is currently Full Professor at the Federal University of Santa Maria, works in the Pharmacy area, with an emphasis on Biopharmaceutical Control and Evaluation of pharmaceutical products, acting mainly on the following themes: development and validation of analytical methods, studies of in vitro release of pharmaceutical formulations, in vitro studies using cell models to determine the activity and toxicological profile of nanostructured systems. She is the master's and doctoral advisor of the Graduate Program in Pharmaceutical Sciences at UFSM. She is an Ad-hoc reviewer of scientific journals and funding agencies and is a member of the Brazilian Association of Pharmaceutical Sciences (ABCF). She was Research Coordinator of the Dean of Graduate Studies and Research at the Federal University of Santa Maria in the 2014-2017 period. She is currently a substitute coordinator of the PPGCF at UFSM. Department of Pharmaceutical Sciences, Health Sciences Center, Federal University of Santa Maria (UFSM), Av. Roraima No. 1000, building 26, Camobi, Zip code 97105-900, Santa Maria, RS, Brazil.

Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Av. Roraima nº 1000, prédio 26, Camobi, CEP 97105-900, Santa Maria, RS, Brasil.

Daniele Rubert Nogueira Librelotto, Universidade Federal de Santa Maria, Santa Maria, RS

PhD (2013) and Master (2010) in Investigación, Desarrollo y Control de Medicamentos by Universitat de Barcelona, Spain. She has a master's degree in Pharmaceutical Sciences (2009) and a degree in Industrial Pharmacy (2006) from the Federal University of Santa Maria (UFSM). In addition, she completed a post-doctorate with the Graduate Program in Pharmaceutical Sciences at UFSM (2013-2021), with an emphasis on nanotechnology and in vitro models for assessing antitumor activity. She is currently an Adjunct Professor at the Federal University of Santa Maria, in the Department of Industrial Pharmacy. She has experience in drug quality control, nanotechnology and in vitro toxicology. In research, she has experience in alternative in vitro methods for assessing toxicity and pharmacological activity, working especially on topics such as cell cultures, cytotoxicity, anti-tumor and antioxidant activity in vitro and interaction tests of pH-sensitive biocompatible surfactants with cell membranes. She also has experience in nanotechnological development of formulations and in drug quality control and bioequivalence studies, with an emphasis on the development and validation of analytical and bioanalytical methods by liquid chromatography and capillary electrophoresis. Department of Pharmaceutical Sciences, Health Sciences Center, Federal University of Santa Maria (UFSM), Av. Roraima No. 1000, building 26, Camobi, Zip code 97105-900, Santa Maria, RS, Brazil.

Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria (UFSM), Av. Roraima nº 1000, prédio 26, Camobi, CEP 97105-900, Santa Maria, RS, Brasil.

Frederico Luiz Reis, Universidade Federal de Santa Maria, Santa Maria, RS

Graduated in Chemistry, full degree (2016), Master in Organic Chemistry (2018), with emphasis on natural products, from the Federal University of Santa Maria (UFSM). He is currently part of the Lab group. RMN-UFSM, where he is a nuclear magnetic resonance operator (performing several 1D, 2D analyzes, as well as maintenance), doctoral student in Organic Chemistry at the Federal University of Santa Maria. He has experience in the area of Organic Chemistry, with an emphasis on Chemistry of Natural Products, structural modification of NPs, Biotechnology (with an emphasis on medicinal plants) and antimicrobial activities. Acting mainly on the following themes: extraction, isolation, structural determination, antimicrobial activity, biotechnology techniques aimed at the propagation of Tabernaemontana catharinensis and quantification by nuclear magnetic resonance of natural products. Department of Chemistry, Federal University of Santa Maria, Santa Maria, Av. Roraima No. 1000, building 21, Camobi, Zip code 97105-900, Santa Maria, RS, Brazil.

Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, Av. Roraima nº 1000, prédio 21, Camobi, CEP 97105-900, Santa Maria, RS, Brasil.

Liziane Maria Barassuol Morandini, Universidade Federal de Santa Maria, Santa Maria, RS

 

Graduated in Pharmacy and Biochemistry - Food Technology from the Federal University of Santa Maria (1993) and Master in Food Science and Technology from the Federal University of Santa Maria (2001), in the Microbiology Area. I have a doctorate from the Graduate Program in Chemistry - UFSM, with the CAPES 6 concept. I developed projects in the area of Natural Products, with extraction, isolation and identification of bioactive molecules with pharmacological properties in ectomycorrhizal fungi. I executed a Technological Development project with Ectomycorrhizal fungi aiming at the identification of new molecules with the potential to implement Eucalyptus growth. I currently co-mentor master's and doctoral students in the extraction of bioactive molecules in fungi. I do activities in Clinical Pharmacy. Department of Chemistry, Federal University of Santa Maria, Santa Maria, Av. Roraima No. 1000, building 21, Camobi, Zip code 97105-900, Santa Maria, RS, Brazil.


Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, Av. Roraima nº 1000, prédio 21, Camobi, CEP 97105-900, Santa Maria, RS, Brasil.

 

Ademir Farias Morel, Universidade Federal de Santa Maria, Santa Maria, RS

 

Graduated in Chemistry from the Federal University of Santa Maria (1974), Master in Chemistry from the State University of Campinas (1979), PhD in Chemistry from the Tuebingen University (1985), Post-doctorate from the University of Hamburg, Germany. He is currently an associate professor at the Federal University of Santa Maria. He has experience in the area of Chemistry, with an emphasis on Organic Chemistry of Natural Products, acting mainly on the following topics: isolation, structural determination, synthesis, antimicrobial activity, essential oil, stereochemistry, synthesis of cyclodextrin derivatives and use in CG. Department of Chemistry, Federal University of Santa Maria, Santa Maria, Av. Roraima No. 1000, building 21, Camobi, Zip code 97105-900, Santa Maria, RS, Brazil.


Departamento de Química, Universidade Federal de Santa Maria, Santa Maria, Av. Roraima nº 1000, prédio 21, Camobi, CEP 97105-900, Santa Maria, RS, Brasil.

Claudia Severo da Rosa, Universidade Federal de Santa Maria, Santa Maria, RS

 

Graduated in Chemistry from the Federal University of Santa Maria (1986), Master in Food Science and Technology from the Federal University of Santa Maria (2000) and doctorate in Food Sciences from the Federal University of Santa Catarina (2008)). Associate Professor at the Federal University of Santa Maria. He has experience in the field of Food Science and Technology, with an emphasis on Food Chemistry, working mainly on the following topics: meats, fruits, cereals, sensory analysis. Department of Technology and Food Science, Center for Rural Sciences, Federal University of Santa Maria, (UFSM), Av. Roraima No. 1000, building 42, Camobi, Zip code 97105-900, Santa Maria, RS, Brazil.


Departamento de Tecnologia e Ciência dos Alimentos, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Av. Roraima nº 1000, prédio 42, Camobi, CEP 97105-900, Santa Maria, RS, Brasil.

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Published

2021-07-29

How to Cite

Flores, D. C. B., Boeira, C. P., Rolim, C. M. B., Librelotto, D. R. N., Reis, F. L., Morandini, L. M. B., Morel, A. F., & Rosa, C. S. da. (2021). Phenolic compounds, antimicrobial activity, cytotoxicity, and identification of phytochemicals present in Inga marginata Willd seeds. Ciência E Natura, 43, e78. https://doi.org/10.5902/2179460X65941

Issue

Vol. 43 (2021): Continuous Publication

Section

Chemistry

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