Evaluation of antioxidant, antimicrobial, toxicological, and larvicidal activity of Psychotria fractistipula L.B. Sm., Klein & Delprete
DOI:
https://doi.org/10.5902/2179460X39729Keywords:
Psychotria, Antioxidant, Antimicrobial, ToxicologicalAbstract
The objective of this study was to assess the potential antioxidant, antimicrobial, and toxicological properties of crude extracts and fractions obtained from the leaves and stem of Psychotria fractistipula L.B. Sm., Klein & Delprete. The content of phenolic compounds varied significantly between samples (783.70–78.22 GAE mg/g), with the highest concentrations being in the ethyl acetate fraction of the leaves and stem (679.39 and 783.70 GAE mg/g, respectively). The latter yielded also the best IC50 of the DPPH radical, which amounted to 9.48 and 4.75 µg/mL, respectively; whereas other samples ranged up to 156.64 µg/mL. Similarly, phosphomolybdenum activity varied between 90.17% and 16.00%, with the ethyl acetate fractions of the leaves and stem corresponding to 90.17% and 87.37%, respectively. Antimicrobial activity was elevated in the leaves crude extract (Staphylococcus aureus, 62.5 µg/mL), leaves ethyl acetate fraction (S. aureus, 31.25 µg/mL; Enterococcus faecalis, 62.4 µg/mL), and the stem ethyl acetate fraction (S. aureus, 31.25 µg/mL; Pseudomonas aeruginosa, 62.5 µg/mL). Hemolytic activity was high in the chloroform fractions of the leaves (1000 µg/mL) and stem (500 µg/mL). Larvicidal activity against Aedes aegypti was observed in the hexane fraction of the stem (LC50, 297.44 µg/mL). The ethyl acetate fractions of the stem and leaves were toxic to Artemia salina, with LC50 values of 277.91 and 933.89 µg/mL, respectively. These results indicate that P. fractistipula may constitute an unexplored source of natural antioxidants and antimicrobials with low toxicity.
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Ayres M C, Brandão M S, Vieira-Júnior G M, Mensor J C A S, Silva H B, Soares M J S, Chaves M H. Atividade antibacteriana de plantas úteis e constituintes químicos da raiz de Copernicia prunifera. Rev. Bras. Farmacog. 2008; (18): 90-97.
Banerjee A, Kunwar A, Mishra B, Priyadarsini K I. Concentration dependent antioxidant/pro-oxidant activity of curcumin studies from AAPH induced hemolysis of red blood cells. Chem.-Biolog. Interact., 2008; (172): 134-139.
Balasundram N, Sudram K, Samman S, Phenolic compounds in plants and agri-industrial by-products: Antioxidant activity, occurrence, and potential uses. Food Chem. 2006; (99): 191-203.
Benevides P J C, Young M C M, Bolzani V da S. Biological Activities of Constituentsfrom Psychotria spectabilis. Pharmac. Biol., 2005; (42): 565-569.
Carvalho J L S, Miguel, M D, Miguel O G, Dadalt R C. PI 0601703-7A Processo de obtenção de extratos hidroalcoólico, extratos secos e derivados do agrião (Nasturtium officinale) e espécies medinais afins, com modificações introduzidas em equipamento Soxhlet para aplicações na industria, área farmaceutica, cosmética, alimenticia e afins, Brasil. 2006
Chobot V, Huber C, Trettenhahn G, Hadacek F. (±)-Catechin: Chemical Weapon, Antioxidant, or Stress Regulator? J Chem Ecol. 2009; (35): 980-996.
Clinical and Laboratory Standards Institute (CLSI), M07-A8: Methods for dilution antimicrobial susceptibility tests forbacteria that grow aerobically. Wayne, PA, USA. 2008.
Da Rosa E A, Silva B C, Da Silva F M, et al Flavonoids and antioxidant activity in Palicourea rigida Kunth, Rubiacea. Braz. Jour. of Pharmac., 2010; (20): 484–488.
Daglia M, Polyphenols as antimicrobial agents, Current Opinion in Biot. 2011; (23): 174-181.
Davis A P, Govaerts R, Bridson D M, Ruhsam M, Moat J, Brummitt N A. A Global Assessment of Distribution, Diversity, Endemism, and Taxonomic Effort in the Rubiaceae 1. An. Mis. Bot. Gard. 2009; (96): 68–78.
Dewick P M. Medicinal Natural Products: A Biosynthetic approach. 2 ed editora John Willey & sons, LTD. 2002.
Dogan A, Otlu S, Çelebi O, Kiliçle A P, Saglam A G, Dogan A N C, Mutlu N. An investigation of antibacterial effects of steroids Turk J Vet Anim Sci. 2017; (41) 302-305.
Dormas W C, Oliveira T T, Rodrigues-das-dores R G, Santos A F, Nagem T J. Flavonóides: potencial terapêutico no estresse oxidativo. Rev. Ciênc. Farmac. Bás. Apli. 2008; (28): 241–249,
Farias F M, Passos M D, Arbo M D, Barros D M, Gottfried C, Steffen V M, Henriques K. Strictosidic acid isolated from Psychotria myriantha Mull. Arg (Rubiaceae), decreases seretonin level in rat hippocampus. Fitot. 2012; (83): 1138-1143.
Finney D J. Probit Analysis. Cambridge University Press, Cambridge. 1971.
Formagio A S N, Volobuff C R F, Santiago M, Cardoso C A L, Vieira M C, Pereira Z V, Evaluation of Antioxidant activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts. Antiox. 2014; (3): 745-757.
Fowler Z L, Shah K, Panepinto J C, Jacobs A, Koffas M A G., Development of non-natural flavanones as antimicrobial agents. PLoS One 2001; (6): 1-5.
Giang P M, Son H V, Son T P. Study on the chemistry and antimicrobial activity of Psychotria reevessi Waal. (Rubiaceae). Vietnam J. of Chem. 2007; (45): 628-633.
Gosh A, Chowdhury N, Chandra G. Plant extracts as potential mosquito larvicides. Indian J. Med. Res. 2012; (135): 581-589.
Hendra R, Ahmad S, Sukari A, Shukor M Y, Oskoueian E. Flavonoid Analyses and Antimicrobial Activity of Various Parts of Phaleria macrocarpa (Scheff.) Boerl Fruit. Int. J. of Mol. Sci. 2011; (12): 3422-3431.
Jagetia G C, Baliga M S, Venkatesh P, Ulloor J N, Mantena K S, Genebriera J, Mathuram V, Evaluation of the cytotoxic effect of monoterpene indole alkaloid echitamine in-vitro and in tumor-bearing mice J. Pharm. Pharmacol. 2005; (57): 1213–1219.
Junior H M S, Oliveira D F, Carvalho D A, Pinto J M A, Campos V A C, Mourão, A R B, Pessoa C, Moraes M O, Costa-lotufo L V. Evaluation of native and exotic Brazilian plants for anticancer activity, J. Nat. Med. 2010; (64): 231-238.
Kerber V A, Passos C S, Verli H, Fett-neto A G, Quirion J P, Henriques A. Psychollate, a glucosidic monoterpene indole alkaloid from Psychotria umbellata. J. of Nat. Prod. 2008; (70): 697-700.
Katajima M. Chemical studies on monoterpenoid indole alkaloids from medicinal plant resources Gelsemium and Ophiorrhiza J. Nat. Med. 2007; (61): 14–23
Kurek A, Grudniak A M. Kraczkiewicz-Dowjat, A., Wolska, K.I., New antibacterial therapeutics and strategies. Pol. J. Microb., Warsaw, 2011; (60): 3-12.
Lima É R, Moreira L S, Facundo V A, Jardim I S, Teles C B G., Avaliação da bioatividade do extrato etanólico e triterpeno lupano obtidos de combretum leprosum contra introdução microorganismos. Sab. Cient. 2011; (3): 53–69.
Lima M C J de S, Soto-Blanco B. Poisoning in goats by Aspidosperma pyrifolium Mart.: Biological and cytotoxic effects. Toxicon, 2010; (55): 320-324
Lopes SO, Moreno PRH, Henriques AT. Growyh characteristics and chemical analysis of Psychotria caerhagenensis cell suspension cultures. Enzyme and Microb Tecnol. 2000; (26): 259-264.
Lopez A, Rico M, Rivero A, de Tangil M S. The effects of solvents on the phenolic contents and antioxidant activity of Stypocaulon scopariumalgae extracts. Food Chem. 2011; (125): 1104–1109
Lu Q, WANG J, KONG L. Chemical constituents from Psychotria yunnanensis and its chemotaxonomic study. Biochemical Systematics and Ecology, 2014 (52): 20–22.
Mariyammal R, Kavimani S. Anti-Inflammatory Activity of Methanol Extract of the Whole Plant of Psychotria. Int. J. of Pharm. Res. & Rev. 2013; (2): 1–5.
Mclaughlin J L, Rogers L L, Anderson J E. The Use of Biological Assays to Evaluate Botanicals. Drug Inf. J. 1988; (32): 513-524.
Mensor L L, Menezes F S, Leitão G G, Reis A S, dos Santos T C, Coube C S, Leitão S G, Screening of brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phyt. Res. 2001; (15): 127-130.
Meyer B N, Ferrigni N R, Putnam J E, Jacobsen L B, Nichols D E, McLaughlin J L. Brine Shrimp: a convenient general bioassay for active plant constituints. Plant. Méd. 1982; (45): 31-34.
Moreira F P M, Coutinho V, Montanher A B P, Caro M S B, Brighente I M C, Pizzolatti M G. Monache, F.D., Flavonoids and triterpenes from Baccharis pseudotenuifolia – Bioactivity on Artemia salina. Quim. Nova 2003; (26): 309–311.
Moreno B P, FioruccI L L R, Do Carmo M R B, Sarragiotto M H, Baldoqui D C. Terpenoids and a coumarin from aerial parts of Psychotria vellosiana Benth. (Rubiaceae). Bioch. System. and Ecol., 2014; (56): 80–82.
Oliveira C F, Oliveira V B, Bobek V B, Rech K S, Betim F C M, Dias J F G, Zanin S M W, Miguel O G M, Miguel M D. Phytochemical and morpho-anatomical study of the vegetative organs of Psychotria fractistipula L.B.Sm., R.M. Klein & Delprete (Rubiaceae). Braz. J. Pharm. Sci. 2020 (56); ahead of print.
Oliveira C F, Oliveira V B, Oliveira F F, Miguel O G, Miguel M D. Quality control parameters of Psychtria fractistipula L. B. Sm., Klein & Delprete (Rubiaceae): Loss on drying, total ash and phytochemical screening. Vis. Acad. 2014; (15): 17-23.
Paul J H, Maxwell A R, Reynolds W F, Novel bis(monoterpenoid) indole alkaloids from Psychotria bahiensis. J. Nat. Prod. 2003; (66): 752-754.
Prieto P, Pineda M, Aguilar M, Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal. Bioch. 1999; (269): 337–341.
Rahuman A A, Gopalakrishnan G, Venkatesan P, Geetha K, Isolation and identification of mosquito larvicidal compound from Abutilon indicum (Linn.) Sweet. Parasitol Res 2008; (102): 981–988
Saleem M, Nazir M, Ali M S, Hussain H, Lee Y S, Riaz N, Jabbar A. Antimicrobial natural products: an update on future antibiotic drug candidates. Nat. Prod. Rep. Cambridge, 2010; (27): 238-254.
Shaalan E A S, Canyonb D, Younesc M W F, Abdel-Wahaba H, Mansoura A H. A review of botanical phytochemicals with mosquitocidal potential. Environ Intv 2005; (3): 1149–66.
Singleton V L, Orthofer R, Lamuela- Raventós R M. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin Ciocalteau reagent. Meth. in Enzym. 1999; (299): 152- 172.
Siqueira J M, Ziminiani M G, Resende U M, Boaventura M A D. Activity-guided isolation of the constituents from bark of stem of Duguetia glabriuscula - Annonaceae, using Brine Shrimp Lethality test (BSL). Quím. Nova 2001; (24):185-187.
Soares D B S, Duarte L P, Cavalcanti A D, Silva F C, Braga A D, Lopes M T P, Takahashi J A, Vieira-Filho S A, Psychotria viridis: Chemical constituents from leaves and biological properties. An. Acad. Bras. Cienc. 2017; (89): 927-938.
Soares S E. Phenolic acids as antioxidants. Rev. de Nutr., 2002; (15): 71–81.
Sousa C M M, Silva H R, Vieira-JR G M, Ayres M C C, Da Costa C K S, Araújo D S, Cavalcante L C D, Barros E D S, Araújo P B M, Brandão M S, Chaves M H. Total phenolics and antioxidant activity of five medicinal plants. Quím. Nova 2007; (30): 351-355.
Souza A M, Armstrong L, Merino F J Z. In vitro effects of Eugenia pyriformis Cambess., Myrtaceae: Antimicrobial activity and synergistic interactions with Vancomycin and Fluconazole. Afr. J. Pharm. Pharmac. 2014; (8): 862–867.
Souza R K D, Mendonça A C A M, Silva M A P. Ethnobotanical, phytochemical and pharmacological aspects Rubiaceae species in Brazil. Rev. Cubana Plant. Med. 2013; (18): 140–156.
Takayama H, Mori I, Kitajima M, Aimi N, Lajis N H, New type of trimeric and pentameric indole alkaloids from Psychotria rostrata. Org. Lett. 2004; (6): 2945–8.
Taleb-contini S H, Salvador M J, Watanabe E, Ito I Y. Antimicrobial activity of flavonoids and steroids isolated from two Chromolaena species. Braz. J. Pharm. Sci. 2003; (39): 403–408.
Tamokou Jde D, Kuiate JR, Tene M, Kenla Nwemeguela TJ, Tane P. The Antimicrobial Activities of Extract and Compounds Isolated from Brillantaisia lamium. Iran J Med Sci. 2011; (36): 24-31.
Taylor C, Gomes M, Zappi D. Rubiaceae in Plant Species List of Brazil. Botanical Garden of Rio de Janeiro. Available at: http://www.floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB14153. 2015.
Tiwari B K, Valdramidis V P, O'Donnell C P, Muthukumarappan K, Bourke P, Cullen P J. Application of natural antimicrobials for food preservation. J. Agric. Food Chem. 2009; (57): 5987-6000.
WORLD HEALTH ORGANIZATION (WHO) Instructions for determining the susceptibility or resistance of mosquito larvae to insecticides. Geneva. 1981.
WORLD HEALTH ORGANIZATION (WHO). Guidelines for Laboratory and Field Testing of Mosquito Larvicides. World Health Organization, Geneva. 2005.
Yamasaki T, Sato M, Mori T, Maohamed A S A, Fujii K, Tsukioka J. Toxicity of tannins towards the free-living nematode Caenorhaditis elegans and the brine shrimp Artemia salina. J. Nat. Toxins 2002; (11): 166-171.
Yang H, Zhang H, Yang C, Chen Y. Chemical Constituents of Plants from the Genus Psychotria, Chem. & Biod. 2016; (13): 807-820.
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