Contribution to the identification of the species Myrcia hatschbachii D. Legrand (Myrtaceae): anatomical and histochemical analyses

Authors

  • Larissa Junqueira Gatto Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil http://orcid.org/0000-0001-6451-7445
  • Vanessa Barbosa Bobek Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil http://orcid.org/0000-0002-2836-2076
  • Jane Manfron Budel Postgraduate Program in Pharmaceutical Science. State University of Ponta Grossa, Ponta Grossa, PR, Brazil http://orcid.org/0000-0003-1873-2253
  • Natasha Tiemi Fabri Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil http://orcid.org/0000-0003-4237-7626
  • Josiane de Fatima Gaspari Dias Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil http://orcid.org/0000-0002-8548-8505
  • Obdulio Gomes Miguel Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil http://orcid.org/0000-0002-2231-9130
  • Marilis Dallarmi Miguel Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil http://orcid.org/0000-0002-1126-9211

DOI:

https://doi.org/10.5902/2179460X43417

Keywords:

Microscopy, Microchemistry, Morpho-anatomy, Myrtaceae, Scanning electron microscopy.

Abstract

The genus Myrcia is used in folk medicine to treat diabetes. The plants used in folk medicine require morphological and anatomical references to attest to its authenticity. This is the first report of the microscopic study of Myrcia hatschbachii. In this sense, the aim of the present study was to describe the anatomical characteristics, in order to contribute to the species' identification. For anatomical and surface analyses free-hand sections were prepared and observed using optical microscopy, simultaneously some materials were processed and observed under scanning electron microscopy. In addition, histochemical tests were performed. The anatomical features described here correspond with previously reported features found in other species of the genus Myrcia. Furthermore, the following anatomical markers were observed in Myrcia hatschbachii: druses in the spongy parenchyma, concave-convex midrib, heart-shaped petiole, oval-shaped stem, C-shaped vascular bundle in the midrib and open arc shape with invaginated ends in the petiole; phenolic compounds in the phloem and lignified cells in the pith. The results of anatomical and histochemical analyses provide micromorphological and microchemical features that can help in the taxonomy and proper identification of the species.

Author Biographies

Larissa Junqueira Gatto, Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Vanessa Barbosa Bobek, Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Jane Manfron Budel, Postgraduate Program in Pharmaceutical Science. State University of Ponta Grossa, Ponta Grossa, PR, Brazil

Postgraduate Program in Pharmaceutical Science. State University of Ponta Grossa, Ponta Grossa, PR, Brazil

Natasha Tiemi Fabri, Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Josiane de Fatima Gaspari Dias, Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Obdulio Gomes Miguel, Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Marilis Dallarmi Miguel, Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

Postgraduate Program in Pharmaceutical Science. Federal University of Parana, Curitiba, PR, Brazil

References

ALVES MF, NIZIO DAC, SAMPAIO TS, NASCIMENTO JUNIOR AF, BRITO FA, MELO JO, et al. Myrcia lundiana Kiaersk native populations have different essential oil composition and antifungal activity against Lasiodiplodia theobromae. Ind Crops Prod. 2016;85:266-273.

BERLYN GP, MIKSCHE JP. Botanical microtechnique and cytochemistry. Ames: Iowa State University Press; 1976.

BFG - The Brazil Flora Group. Brazilian Flora 2020: innovation and collaboration to meet Target 1 of the Global Strategy for Plant Conservation (GSPC). Rodriguésia. 2018;69:1513-1527.

BOBEK VB, HEIDEN G, OLIVEIRA CF, ALMEIDA VP, PAULA JP, FARAGO PV, et al. Comparative analytical micrographs of vassouras (Baccharis, Asteraceae). Rev. Bras. Farmacogn. 2016;26:665–672.

BRITO NR, LANETTI RA. A saúde pelas plantas e ervas do mundo inteiro. Rio de Janeiro: Ed. Ouro; 1996.

BUDEL JM, RAMAN V, MONTEIRO LM, ALMEIDA VP, BOBEK VB, HEIDEN G, et al. Foliar anatomy and microscopy of six Brazilian species of Baccharis (Astereraceae). Microsc Res Tech. 2018;81:832-842.

CASCAES MM, GUILHON GMSP, ANDRADE EHA, ZOGHBI MGB, SANTOS LS. Constituents and pharmacological activities of Myrcia (Myrtaceae): A review of an aromatic and medicinal group of plants. Int. J. Mol. Sci. 2015;10:23881–23904.

CARDOSO CMV, PROENÇA SL, SAJO MG. Foliar anatomy of the subfamily Myrtoideae (Myrtaceae). Aust. J. Bot. 2009;57:148-161.

DONATO AM, MORRETES BL. Morfo-anatomia foliar de Myrcia multiflora (Lam.) DC. – Myrtaceae. Rev. bras. plantas med. 2011;13:43-51.

FLORA DO BRASIL 2020 [Internet]. Jardim Botânico do Rio de Janeiro. [cited 2020 out 13]. Available from: http://floradobrasil.jbrj.gov.br/reflora/floradobrasil/FB10660.

FOSTER AS. Practical plant anatomy. Princeton: D. Van Nostrand; 1949.

FRANCESCHI VR, NAKATA PA. Calcium oxalate in plants: formation and function. Annu. Rev. Plant Biol. 2005;56:41–71.

FUCHS CH. Fuchsin staining with NaOH clearing for lignified elements elements of whole plants or plants organs. Stain Technol. 1963;38:141-144.

GABE M. Techniques Histologiques. Paris: Masson & Cie; 1968.

GATTO LJ, VEIGA A, GRIBNER C, MOURA PF, RECH KS, MURAKAMI FS, DIAS JFG, MIGUEL OG, MIGUEL MD. Myrcia hatschbachii: antifungal activity and structural elucidation of ellagic and 3-O-methyl ellagic acids. Nat Prod Res 2020;1-4.

GOMES SM, SOMAVILLA NSDN, GOMES-BEZERRA KM, MIRANDA SC, CARVALHO OS, GRACIANO-RIBEIRO D. Anatomia foliar de espécies de Myrtaceae: contribuições à taxonomia e filogenia. Acta bot. bras. 2009;23:223-238.

JOHANSEN DA. Plant Microtechnique. New York: McGraw Hill Book; 1940.

JORGE LIF, AGUIAR JPL, SILVA MLP. Anatomia foliar de pedra hume-caá (Myrcia sphaerocarpa, Myrcia guianensis, Eugenia punicifolia - Myrtaceae). Acta Amaz. 2000;30:49-57.

LANDRUM LR, KAWASAKI ML. The genera of Myrtaceae in Brazil: an illustrated synoptic treatment and identification keys. Brittonia. 1997;49:508-536.

LUCAS EJ, HOLST B, SOBRAL M, MAZINE FF, NIC LUGHADHA E, PROENÇA CEB, COSTA IR, VASCONCELOS TNC. A new tribal classification of tribe Myrteae (Myrtaceae). Syst. Bot. 2019;44:560–569.

LUCAS EJ, MATSUMOTO K, HARRIS AS, LUGHADHA EMN, BENARDINI B, CHASE, MW. Phylogenetics, Morphology, and Evolution of the Large Genus Myrcia s.l. (Myrtaceae). Int. J. Plant Sci. 2011;172:915–934.

METCALFE CR, CHALK L. Anatomy of the Dicotyledons. Oxford: Clarendon Press; 1979.

MIGACZ IP, RAESKI PA, ALMEIDA VP, RAMAN V, NISGOSKI S, MUNIZ GIB, et al. Comparative leaf morpho-anatomy of six species of Eucalyptus cultivated in Brazil. Rev. Bras. Farmacogn. 2018;28:273–281.

O’BRIEN TP, FEDER N, MCCULLY ME. Polychromatic staining of plant cell walls by toluidine blue O. Protoplasma. 1964;59:368-373.

OLIVEIRA F, AKISUE G. Fundamentos de farmacobotânica. São Paulo: Atheneu; 1997.

RETAMALES HA, SCHARASCHKIN T. Comparative leaf anatomy and micromorphology of the Chilean Myrtaceae: Taxonomic and ecological implications. Flora. 2015;217:138-154.

RODRIGUES MCM, BORGES LL, MARTINS FS, MOURÃO RHV, CONCEIÇÃO EC Optimization of ultrasound-assisted extraction of phenolic compounds from Myrcia amazonica DC. (Myrtaceae) leaves. Pharmacogn. Mag. 2016;12:9–12.

ROESER KR. [The needle of black pine mass-produced and nature's work of art]. Mikrokosmos. 1972;61:33-36. German.

SANTOS MF, SANO PT, FOREST F, LUCAS E. Phylogeny, morphology and circumscription of Myrcia sect. Sympodiomyrcia (Myrcia s.l., Myrtaceae). Taxon 2016;65:759-774.

SASS JE. Botanical Microtechnique. Ames: Iowa State College; 1951.

SAULLE CC, RAMAN V, OLIVEIRA AVG, MAIA BHLNS, MENEGHETTI EK, FLORES TB, et al. Anatomy and volatile oil chemistry of Eucalyptus saligna cultivated in South Brazil. Rev. Bras. Farmacogn. 2018;28:125–134.

SUGDEN AM. Leaf anatomy in a Venezuelan Montana forest. Bot. J. Linn. Soc. 1985;90:231-241.

WILSON PG, O'BRIEN MM, HESLEWOOD MM, QUINN CJ. Relationships within Myrtaceae sensu lato based on a matK phylogeny. Plant Syst. Evol. 2005;251:3-19.

WILSON P. Myrtaceae. In: Kubitzki, K. (eds.). The families and genera of vascular plants. Flowering plants Eudicots: Sapindales, Cucurbitales, Myrtaceae. Heidelberg: Springer-Verlag; 2011.

WOSCH L, IMIG DC, CERVI AC, MOURA BB, BUDEL JM, SANTOS CAM. Comparative study of Passiflora taxa leaves: I. A morpho-anatomic profile. Rev. Bras. Farmacogn. 2015;25:328-343.

Downloads

Published

2021-08-04

How to Cite

Gatto, L. J., Bobek, V. B., Budel, J. M., Fabri, N. T., Dias, J. de F. G., Miguel, O. G., & Miguel, M. D. (2021). Contribution to the identification of the species Myrcia hatschbachii D. Legrand (Myrtaceae): anatomical and histochemical analyses. Ciência E Natura, 43, e75. https://doi.org/10.5902/2179460X43417

Issue

Section

Biology-Botany

Most read articles by the same author(s)