Recovery of mangrove sediment contaminated with fuel oil by endogenous microbial consortium

Authors

DOI:

https://doi.org/10.5902/2179460X39667

Keywords:

Mangrove, Bioremediation, Microorganisms, Consortium

Abstract

This work aimed to select a microbial consortium enriched with isolated microorganisms of mangrove sediment as to its capacity to recover sediment contaminated by lubricating oil. The promising microorganisms were selected using the colorimetric dichlorophenol indophenol technique (DCPIP) using lubricating oil as the carbon source, to evaluate the emulsifying and enzymatic activity of the microorganisms. The antagonism test was also used for further evaluation of the consortia. The fractional factorial experimental design methodology (2n) was used to establish the process conditions for the subsequent accomplishment of the degradation kinetics of the lubricating oil by the selected microorganisms and consortium. Eight bacteria and three fungi were evaluated, of which five were selected with a 36 h turn of the DCPIP indicator. Eleven microorganisms produce emulsifying substances and five produce enzymes. The results showed that the best consortium was B5F2F4, with a degradation rate of 95% of the phenol at 70 rpm in 250 μL of the oil. The kinetics of oil degradation showed a phenol degradation rate of 65% after 24 days of treatment. The microorganisms are suitable for the degradation of phenol, the main constituent of the oil, and can be used as a recovery model for environments contaminated with hydrocarbons.

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Author Biographies

Amanda Ferreira Santos Silva, Universidade Ceuma, São Luís, MA

Graduada em Engenharia de Petróleo. É membro do grupo de pesquisa Química Tecnológica e Ambiental (CNPq) desenvolvendo pesquisas na área de Biotecnologia e Tecnologias de Processos com experiência em otimização de processos biotecnológicos de interesse industrial, recuperação de áreas degradadas e modelagem computacional através de autômatos celulares (AC's).

Thomas Wendell Fernandes dos Santos, Universidade Ceuma, São Luís, MA

Possui graduação em Engenharia de Petróleo pela Universidade Ceuma

Norma Buarque de Gusmão, Universidade Federal de Pernambuco, Recife, PE

Professora do Departamento de Antibióticos, Universidade Federal de Pernambuco

Persio Alexandre da Silva, Universidade Federal de Pernambuco, Recife, PE

Possui graduação em Engenharia Quimica pela Universidade Federal de Pernambuco

Thais de Melo Castelo Branco, Universidade Ceuma, São Luís, MA

Mestranda em Meio Ambiente pela Universidade Ceuma. Engenheira de Petróleo pela Universidade Ceuma. Pesquisadora no Projeto Avaliação do Potencial de Biorremediação das Comunidades Microbianas de Solos Contaminados com Compostos Hidrocarbônicos, no Laboratório de Microbiologia, Universidade Ceuma.

Rita de Cássia Mendonça de Miranda, Universidade Ceuma, São Luís, MA

Professora da Universidade Ceuma, vinculada aos programas de mestrado em Meio Ambiente como professora permanente

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Published

2020-09-03

How to Cite

Silva, A. F. S., Santos, T. W. F. dos, Gusmão, N. B. de, Silva, P. A. da, Branco, T. de M. C., & Miranda, R. de C. M. de. (2020). Recovery of mangrove sediment contaminated with fuel oil by endogenous microbial consortium. Ciência E Natura, 42, e6. https://doi.org/10.5902/2179460X39667

Issue

Vol. 42 (2020): 40 years special anniversary edition

Section

40 YEARS - Anniversary Special Edition

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