Remotion of the 17α-Ethinylestradiol Hormone (EE2) by Biosorbent (Arachis hypogaea) in Aqueous Solutions: Validation of Analytical Methodology and Adsorption Study

Alley Michael da Silva Procópio, Thayná Aparecida Cais, Waleska Freitas Da Silva, Márcia Matiko Kondo, Flávio Soares Silva, Sandro José de Andrade


This study describes the application of peanut shells, a biodegradable agroindustrial residue as a biosorbent, in the 17α-ethinylestradiol (EE2) removal in water matrices. An analytical method was developed and validated from the high-performance liquid chromatographic technique with fluorescence detection (HPLC-FLD) in a λEx= 230 nm and λEm= 310 nm in the determination of EE2. The evaluated parameters were: selectivity, linearity (R2 = 0.9984 and R = 0.9992), precision (Variation coefficient = 2.90% and 2.95% for the first and second analyst, respectively), accuracy (recovery rate = 100.2 – 110.4%), detection limit (3.4 – 5.0 μg L-1), quantification limit (10.0 – 11.3 μg L-1) and robustness (recovery rate = 98.7 – 115.5%). The chromatographic analysis conditions were: mobile phase (water 2:3 acetonitrile), mobile phase flow (0.5 mL min-1), injection volume (10 µL), column temperature (45ºC) and race time (10 min). An experiment planning (Box-Behnken Model) was carried out for the investigation and verification of the adsorptive capacity of the peanut shells, of which 3 parameters were evaluated (stirring rate, pH and adsorbent mass) on 3 levels. Optimum experimental condition (2 g of adsorbent, pH = 6 and stirring at 500 rpm) with a removal rate of 90% in 24 hours of the process.


17α-ethinylestradiol. endocrine disruptors. emerging pollutants. validation of analytical methodology. Box-Behnken. Removal. Biosorbents. peanut shells

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