Study of biofilms with kefir associated with soy germs (Glycine max (L.) Merril)




Biofilms; , Kefir;, Soy germs;, Isoflavones; , AFM


The aim of this study was to investigate biofilm formation with kefir grains in the presence of soy extract. Kefir grains and soy germs at different concentrations were grown in the culture medium comprising brown sugar solution (40 gl-1) for 20 days. Biofilms that formed in this period were then removed and the pH of the culture medium were measured. Isoflavones of the medium of culture were extracted and quantified by high-performance liquid chromatography (HPLC). The superficial properties of the selected biofilms were analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The culture medium after 20 days was found to have 19.59±3.57 µgl-1 of glycitein and 23.86±2.21 µgl-1 of genistein. The best concentration of kefir grains in order to extract isoflavone was 40 gl-1, with yield levels at 11.67 µgl-1 of glycitein and 17.78 µgl-1 of genistein. The analysis by AFM and SEM confirmed the increased roughness of the biofilm, dependent of the concentration of the amount of kefir grains. It is suggested that the biofilms incorporated the isoflavones and has potential for therapeutic applications in several pathologies wherein it is necessary the antioxidative processes.


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Author Biographies

Antonio Ferreira Oliveira, Instituto Federal do Amapá, Amapá, AP

He completed his Post-Doctorate, with an emphasis on the research of Bioremediation of Inorganic Contaminants, Doctor in Tropical Biodiversity from the universidade federal do Amapá, Master in Microbiology from the Universidade Estadual Paulista

Alexandro Cezar Florentino, Universidade Federal do Amapá, Amapá, AP

Adjunct Professor C Level II at the Universidade Federal Do Amapá. Master's and Doctoral advisor in the Graduate Program in Environmental Sciences and Tropical Biodiversity. Coordinator of the LIGEN Laboratory and Leader of the Research Group on Management of Natural Resources in the Eastern Amazon.

Adriana Santos Valente, Universidade Federal do Amapá, Amapá, AP

Graduation in progress in Pharmacy. Universidade Federal do Amapá, UNIFAP, Brazil

Roberto Messias Bezerra, Universidade Federal do Amapá, Amapá, AP

Doctor in Exact and Earth Sciences from the School of Engineering of São Carlos - Universidade de São Paulo, USP/São Carlos. Has experience in Chemistry, with emphasis on Electrochemistry.

Robert Maguiña Zamora, Universidade Federal do Amapá, Amapá, AP

Adjunct Professor at UNIFAP, Master in Physics and Doctor in Physics from the Pontifícia Universidade Católica do Rio de Janeiro (2005). Has experience in Condensed Matter Physics, with emphasis on Surface Physics.

Adriana Ferreira Maciel, Universidade Federal do Amapá, Amapá, AP

Graduated in Chemistry from the Universidade Estadual do Mato Grosso do Sul, Specialization in Chemistry from the Universidade Federal de Lavras/UFLA. She is currently a PhD student in Biotechnology at BIONORTE. It works in bioprospecting and generation of bioproducts.

Jose Tavares Carvalho, Universidade Federal do Amapá, Amapá, AP

Doctor in Drugs and Medicines from the Universidade de São Paulo. Postdoctoral fellow at IFP-Berlin-Germany. Professor at the Universodade Federal do Amapá. It works in the area of ​​production of new drugs.


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How to Cite

Oliveira, A. F., Florentino, A. C., Valente, A. S., Bezerra, R. M., Zamora, R. M., Maciel, A. F., & Carvalho, J. T. (2021). Study of biofilms with kefir associated with soy germs (Glycine max (L.) Merril). Ciência E Natura, 44, e47.

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