Evaluation of detection of bacterial contamination in platelet concentrates using the quantitative bacteriological and reduction the concentration of glucose and ph
DOI:
https://doi.org/10.5902/223658342517Keywords:
PH; Glucose; Bacterial contamination; Platelet concentrates; Microbiological culture.Abstract
Background: Bacterial contamination in platelet concentrates (PCs) is a critical problem due to the risk of septic events associated with transfusion. The microbiological culture of the PCs present some challenges as the small volume of inoculum and time to release of results. In this study we evaluated the performance in the detection of contamination in 79 samples PCs from the Blood Center of the State of Rio Grande do South of Santa Maria in 2010 by two different methodologies. Methods: Quantitative bacteriological: seeding of 100 µL of PCs on blood agar; and metabolic markers: measurements of glucose and pH by reagent strips. Results: In all analysis of this study the glucose showed positive values and 91.13% remained above pH 7.5 and 8.87% below 7.0. At 5° day 83.55% of the samples were negative glucose. There was no bacterial growth in any of the samples with initial pH acid. The rate of bacterial contamination was 1.27% Staphylococcus epidermidis). Conclusion: We conclude that metabolic markers used did not allow the detection of bacterial contamination, since the positive sample was not related to the acidification of pH and consumption of glucose.Downloads
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