PHOSPHORUS DOSES DETERMINE THE PREVALENCE OF NATIVE ARBUSCULAR MYCORRHIZAL FUNGI IN Araucaria angustifolia
DOI:
https://doi.org/10.5902/198050987562Keywords:
AMF, diversity, spore density, Brazil PineAbstract
http://dx.doi.org/10.5902/198050987562A greenhouse experiment was installed with bait cultures to extract the AMF species present in a rhizosphere soil sample of a native Araucaria angustifolia forest in Campos do Jordão, Brazil. The experimental design was completely randomized, with four increasing phosphorus doses (0, 20, 50, and 150 mg kg-1, as triple superphosphate), with five replicates, the bait plant was araucaria, and all pots were inoculated with 100g of rhizospheric soil collected in an araucaria forest. After twelve months the spores were extracted, counted and identified, and the percent root colonization was also determined. When taking all four P doses into account, eleven FMA species could be identified: Acaulospora bireticulata, A. morrowiae, Acaulospora sp., Entrophospora colombiana, Gigaspora margarita, Glomus diaphanum, G. etunicatum, G. macrocarpum, Scutellospora calospora, S. gilmorei, and S. pellucida. There was no effect of the P dose on the total amount of spores neither on the percent root colonization. However, the correspondence analysis showed that the different AMF species were selectively associated mostly to either one or another P dose.
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References
BEVER, J. D. et al. Arbuscular mycorrhizal fungi: more diverse than meets the eye, and the ecological tale of why. Bioscience, v. 51, p. 923-931, 2001.
DE MIRANDA, J. C. C.; HARRIS, P. J. The effect of soil phosphorus on the external mycelium growth of arbuscular mycorrhizal fungi during the early stages of mycorrhizal formation. Plant and Soil, v. 166, p. 271-280, 1994.
DOUDS, D. D.; MILLNER, P. Biodiversity of arbuscular mycorrhizal fungi in agroecosystems. Agriculture, Ecosystems & Environment, v.74, p.77-93, 1999.
EZETA, F. N.; SANTOS, O. M. Benefício da introdução de endomicorriza eficiente na utilização de nutrientes em latossolos do sul da Bahia, Revista Brasileira de Ciência do Solo, v. 4, p. 13-17, 1980.
GIOVANNETTI, M.; MOSSE, B. An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytologist, v. 84, p.489-500, 1980.
GRAHAM, J. H et al. Development of external hyphae by different isolates of mycorrhizal Glomus spp. in relation to root colonisation and growth of Troyer citrange. New Phytologist, v. 91, p.183-189, 1982.
HOAGLAND, D. R.; ARANON, D. I. The water-culture method for growing plants without soil. California Agricultural Experimental Station. 1938.
INVAM (2004) International Culture Collection of Vesicular and Arbuscular Mycorrhizal Fungi. Species Description. Morgantown, West Virginia Agriculture and Forestry Experimental Station. Home page. [http: invam.caf.wvu.edu]
KAHILUOTO, H. et al. Promotion of AMF utilization through reduced P fertilization 2. Field studies. Plant and Soil, v. 231, p. 65-79, 2001.
MOREIRA, M. et al. Arbuscular mycorrhizal fungal communities in native and in replanted Araucaria forest: a case study. Scientia Agricola, v. 66, p. 677-684, 2009.
MOREIRA, M. et al. Spore density and root colonization by arbuscular mycorrhizal fungi in preserved or disturbed Araucaria angustifolia (Bert.) O. Ktze. ecosystems. Scientia Agrícola, v. 63, p. 380-385, 2006.
MOREIRA, M. et al. Sporulation and diversity of arbuscular mycorrhizal fungi in Brazil Pine in the field and in the greenhouse. Mycorrhiza, v. 17, p. 519-526, 2007b.
MOREIRA. M. et al. Biodiversity and distribution of arbuscular mycorrhizal fungi in Araucaria angustifolia forest. Scientia Agrícola, v. 64, p. 393-399, 2007a.
MOREIRA-SOUZA, M. et al. Arbuscular mycorrhizal fungi associated with Araucaria angustifolia (Bert.) O. Ktze. Mycorrhiza, v.13, p. 211-215, 2003.
MOREIRA-SOUZA, M.; CARDOSO, E. J. B. N. Dependência micorrízica de Araucaria angustifolia (Bert.) O. Ktze. sob doses de fósforo. Revista Brasileira de Ciência do Solo, v. 26, p. 905-912, 2002.
MOREIRA-SOUZA, M.; CARDOSO, E. J. B. N. Practical method for germination of Araucaria angustifolia (Bert.) O. Ktze. seeds. Scientia Agrícola, v. 60, p. 389-391, 2003.
MORTON, J. B. et al. Germplasm in the international Collection of Arbuscular and Vesicular Arbuscular Mycorrhizal Fungi (INVAM) and procedures for culture development, documentation and storage. Mycotaxon, v. 48, p.491-528, 1993.
NEUMANN, E.; GEORGE, E. Colonisation with the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. & Gerd.) enhanced phosphorus uptake from dry soil in Sorghum bicolor (L.). Plant and Soil, v. 261, p. 245-255, 2004.
PICONE, C. Diversity and abundance of arbuscular-mycorrhizal fungus spores in tropical forest and pasture. Biotropica, v. 32, p. 734-750, 2000.
ROSS, J. P.; HARPER, J. A. Effect of endogone mycorrhiza on soybean yields. Phytopathology, v.60, n. 1, p. 552-556, 1970.
SAS Institute. SAS/STAT. Release 6.2. Cary: SAS Institute Inc., 1996.
SCHENCK, N. C.; PÉREZ, Y. Manual for the identification of VA mycorrhizal fungi, 3rd ed. Gainesville: Synergistic, 1990.
SCHENCK, N. C.; SMITH, G. S Responses of six species of vesicular-arbuscular mycorrhizal fungi and their effects on soybean at four soil temperatures, New Phytologist, v. 92, n. 2, p.193-201, 1992.
SCHÜβLER, A. et al. Analysis of partial Glomales SSU rRNA gene sequence: implications for primer design and phylogeny. Mycology Research, v.105, p. 05-15, 2001.
SIEVERDING, E. Vesicular arbuscular mycorrhiza management in tropical agrosystems. Eschborn, Germany: GTZ, 1991. 371 p.
SILVEIRA, A. P. D. Ecologia de fungos micorrízicos arbusculares. In: MELO, J. L. (ed). Ecologia microbiana. Jaguariúna: Embrapa, 1998. p. 61-83.
SIQUEIRA, J. O. et al. Mycorrhizal colonization and mycotrophic growth of native woody species as related to successional groups in southeastern Brazil. Forest Ecology and Management, v. 107, p. 241-252, 1998.
STUTZ, J. C.; MORTON, J. B. Successive pot cultures reveal high species richness of arbuscular endomycorrhizal fungi in arid ecosystems, Canadian Journal Botany, v. 74, p. 1883-1889, 1996.
SUBRAMANIAN, K. S. et al. Response of field grown tomato plants to arbuscular mycorrhizal fungal colonization under varying intensities of drought stress. Scientia Horticulturae, v. 107, p. 245-253, 2006.
TCHABI, A. et al. Arbuscular mycorrhizal fungal communities in sub-Saharan savannas of Benin, West Africa, as affected by agricultural land use intensity and ecological zone. Mycorrhiza, v. 18, p. 181-195, 2008.
ter BRAAK, C. J. F. Canonical correspondence analysis: a new eigenvector technique for multivariate direct gradient analysis. Ecology, v. 67, p. 1167-1179, 1986.
ter BRAAK, C. J. F.; SMILAUER, P. CANOCO Reference Manual and User’s Guide to Canoco for Windows: software for canonical community ordination (version 4). New York: Microcomputer Power. 1998.
THOMSON, B. D. et al. The effect of long-term applications of phosphorus fertilizer on populations of vesicular-arbuscular mycorrhizal fungi in pastures. Australian Journal of Agriculture Research, v. 43, p. 1131-1142, 1992.
TRUFEM, S. F. B. Aspectos ecológicos de fungos micorrízicos vesículo-arbusculares da mata tropical úmida da Ilha do Cardoso, SP, Brasil. Acta Botanica Brasilica, v. 4, n. 2, p. 31-45, 1990.
VIERHEILIG, H. et al. Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi. Applied and Environmental Microbiology, v. 64, p. 5004-5007, 1998.
