Development, characterization and cytogene-ecotoxicological bioassay of different free and nanoestrutured formulations containing trans-anethole
DOI:
https://doi.org/10.5902/2179460X73826Keywords:
Nanotechnology, Biotechnology, Toxicity, AnetholeAbstract
Trans-anethole is an aromatic compound found in large amounts in the essential oil of various plants, such as star anise. It is recognized for its culinary and traditional medicinal uses. Despite its potential, the low solubility, and complex characteristics, hinder its absorption in the human body. To overcome this challenge, nanotechnology has emerged as a promising solution. In this study, nanocapsules and nanoemulsions containing trans-anethole were developed and characterized for physical-chemical parameters, including particle diameter, zeta potential, pH, polydispersity index, encapsulation rate, and content stability over a 90-day period. Biological assessments were conducted using cytogenotoxicity tests on PBMC cells, and a toxicological bioassay with Artemia salina. The results demonstrated that the nanostructured systems remained stable for 90 days under refrigeration, exhibiting compatible pH, polydispersity index, zeta potential, and particle diameter for biomedical applications, while also exhibiting no cytotoxic or genotoxic activity. The toxicological assays revealed significant protection against the toxicity of trans-anethole when delivered in the nanostructured systems, as compared to its free form.
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