Antioxidant potential and synthesis of copper oxide nanoparticles from plant extracts of Piper amalago L. (Piperaceae)
Keywords:Phenolic compounds, Free radicals, Green synthesis
Piper amalago L. popularly known as jaborandi-manso, jaborandi-falso and jaborandinhandi is widely used in the Brazilian folk medicine for treating urinary calculus diseases. The objective of this study was to evaluate the antioxidant activity and determine the levels of phenols, flavonoids and total tannins of the ethanolic and aqueous extracts of P. amalago leaves, as well as to synthesize and characterize copper oxide nanoparticles using the aqueous extract, in addition to evaluate its antioxidant activity. Quantification of total phenols, flavonoids and tannins was performed by spectrophotometry in the visible region and the antioxidant activity was evaluated by the DPPH• free radical scavenging method. The synthesis of nanoparticles was prepared with a solution containing aqueous extract of the leaves and copper salt. Subsequently, the characterization was performed by microscopy and FTIR and UV-Vis spectroscopy. Both extracts showed antioxidant activity; the ethanol extract was the most active, reducing the DPPH• at a concentration of 100 μg.mL-1 by more than 90%. This extract also presented the highest levels of total phenols (199.17 ± 4.11mg GAE/g extract), total flavonoids (30.84 ± 0.59 mg QE/g extract) and total tannins (158.30 ± 4.83 mg TAE/g extract). The generated nanoparticles showed no great capacity to reduce DPPH•. The results suggest that the antioxidant potential evidenced by P. amalago extracts is mainly related to the presence of phenolic compounds, such as flavonoids and tannins, recognized as antioxidants and confirmed the green synthesis of copper oxide nanoparticles, but with the lowest antioxidant potential.
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