Antioxidant potential and synthesis of copper oxide nanoparticles from plant extracts of Piper amalago L. (Piperaceae)

Authors

DOI:

https://doi.org/10.5902/2179460X69341

Keywords:

Phenolic compounds, Free radicals, Green synthesis

Abstract

Piper amalago L. popularly known as jaborandi-manso, jaborandi-falso and jaborandinhandi is widely used in the Brazilian folk medicine for treating urinary calculus diseases. The objective of this study was to evaluate the antioxidant activity and determine the levels of phenols, flavonoids and total tannins of the ethanolic and aqueous extracts of P. amalago leaves, as well as to synthesize and characterize copper oxide nanoparticles using the aqueous extract, in addition to evaluate its antioxidant activity. Quantification of total phenols, flavonoids and tannins was performed by spectrophotometry in the visible region and the antioxidant activity was evaluated by the DPPH• free radical scavenging method. The synthesis of nanoparticles was prepared with a solution containing aqueous extract of the leaves and copper salt. Subsequently, the characterization was performed by microscopy and FTIR and UV-Vis spectroscopy. Both extracts showed antioxidant activity; the ethanol extract was the most active, reducing the DPPH• at a concentration of 100 μg.mL-1 by more than 90%. This extract also presented the highest levels of total phenols (199.17 ± 4.11mg GAE/g extract), total flavonoids (30.84 ± 0.59 mg QE/g extract) and total tannins (158.30 ± 4.83 mg TAE/g extract). The generated nanoparticles showed no great capacity to reduce DPPH•. The results suggest that the antioxidant potential evidenced by P. amalago extracts is mainly related to the presence of phenolic compounds, such as flavonoids and tannins, recognized as antioxidants and confirmed the green synthesis of copper oxide nanoparticles, but with the lowest antioxidant potential.

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Author Biographies

Mayara Cristina Neves Abel, Universidade Estadual de Mato Grosso do Sul, Dourados, MS

Graduated in the Biological Sciences course (2015-2018), at the State University of Mato Grosso do Sul (UEMS), Mundo Novo Unit/MS. Master in Natural Resources (2019-2021), from the same university, Unit of Dourados/MS. Doctoral student in Natural Resources at the same Higher Education Institution.

Etenaldo Felipe Santiago, Universidade Estadual de Mato Grosso do Sul, Dourados, MS

872 / 5.000   Resultados de tradução Graduated in Biological Sciences from the Universidade Estadual Paulista Júlio de Mesquita Filho - UNESP Campus of Rio Claro-SP (1993), Master in Biological Sciences (Plant Biology) - Department of Botany (1997) and PhD in Biological Sciences (Plant Biology) from the State of São Paulo Júlio de Mesquita Filho - UNESP Campus of Rio Claro-SP (2002). He is currently a professor at the State University of Mato Grosso do Sul-UEMS-Dourados-MS, advisor in the Master's and Doctorate in the Graduate Program in Natural Resources-PGRN. Has experience in Botany, with emphasis on Plant Ecophysiology, working mainly on the following topics: plant responses to stress, native essences, germination, morphology, initial development of seeds and seedlings, replacement of plant cover in degraded areas, environmental assessment.

Ana Francisca Gomes Silva, Universidade Estadual de Mato Grosso do Sul, Mundo Novo, MS

Possui graduação em Química pela Universidade Federal de Mato Grosso do Sul (1992),
Mestrado em Química pela Universidade Federal de Mato Grosso do Sul (2002) e
Doutorado em Química pela Universidade Federal do Mato Grosso do Sul (2010).
Atualmente é professora da Universidade Estadual de Mato Grosso do Sul. Tem
experiência na área de Química Orgânica, com ênfase em Química dos Produtos Naturais.

Jonas da Silva Mota, Universidade Estadual de Mato Grosso do Sul, Dourados, MS

Graduated in Chemistry from the Federal University of Mato Grosso do Sul (1995), Master in Sustainable Development from the University of Brasília (2001) and Doctor in Chemistry from the Universidade Estadual Paulista Júlio de Mesquita Filho de Araraquara (2007). He is currently an assistant professor and coordinator of the Chemistry Course-Uems-Unit de Ensino de Dourados. He has experience in the field of Chemistry, working mainly in the area of Natural Products.

Montcharles da Silva Pontes, Universidade Estadual de Mato Grosso do Sul, Dourados, MS

Graduated in Biological Sciences from the State University of Mato Grosso do Sul, UEMS (2016), Master in Natural Resources (Environmental Sciences) from the Center for Studies in Natural Resources (CERNA) of the State University of Mato Grosso do Sul (2019). He is currently a regular student in the interdisciplinary Doctoral Program in Natural Resources (Environmental Sciences) at the Center for Studies in Natural Resources (CERNA) of the State University of Mato Grosso do Sul. He has experience in the area of ​​Plant Physiology and Biochemistry with an emphasis on bioenergetics and photosynthesis biophotonics and nanobiotechnology, working mainly in nanotoxicology, through the development of nanoparticles and studies of their interaction with photosynthetic apparatus. He is skilled in optical spectroscopy (spectroanalytical) techniques for characterization of biological materials and nanomaterials, chlorophyll fluorescence and in vivo and in vitro nanotoxicological assays. He is currently working on the development of nanocarriers for the controlled release of photosynthesis-inhibiting herbicides. He is a member of the Plant Resources Study Group (GeRV).

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Published

2022-12-12

How to Cite

Abel, M. C. N., Santiago, E. F., Silva, A. F. G., Mota, J. da S., & Pontes, M. da S. (2022). Antioxidant potential and synthesis of copper oxide nanoparticles from plant extracts of Piper amalago L. (Piperaceae). Ciência E Natura, 44, e59. https://doi.org/10.5902/2179460X69341

Issue

Vol. 44 (2022): Continuous Publication

Section

Chemistry

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