Cytogenotoxicity of shakes added with synthetic microingredients
DOI:
https://doi.org/10.5902/2179460X27881Abstract
The goal of this study was to evaluate the cytotoxicity and genotoxicity of diet shakes of three brands widely marketed in Brazil, named in this study of A, B and C. Assessments were carried out on root meristem cells of Allium cepa at exposure times 24 and 48 hours. The products (treatments) for A and B were evaluated at concentrations of 0.05; 0.10 and 0.20 g/mL, and C at the concentration 0.08; 0.16 and 0.32 g/mL. The diet products at the three concentrations tested, including those suggested as ideal for consumption by the manufacturers, at the two exposure times considered, were found to have significant antiproliferative effect on the meristems evaluated. However, none of the treatments caused significant cellular changes to the tissues of roots. Thus, the products tested were cytotoxic, but not genotoxic, to root meristem cells of A. cepa. Furthermore, it was observed that in all the treatments performed, in the three concentrations, the majority of the dividing cells were at prophase, not being observed cells at anaphase and telophase. Thus, it is suggested to conduct more in-depth studies on the cytotoxicity of these shakes in physiologically more complex bioassays for the verification and deepening of the data obtained here.
Downloads
References
ARAÚJO, L. M. P.; FORTES, R. C.; FAZZIO, D. M. G. Análise do uso de dietas da moda por indivíduos com excesso de peso. Journal Health Science Institute, v. 31, n. 4, p. 388-391, 2013.
AYRES, M.; AYRES, J. R. M.; AYRES. D. L.; SANTOS, A. S. BioEstat 5.0 - Aplicações Estatísticas nas Áreas das Ciências Biológicas e Médicas. Brasília: CNPq, 2007.
BRASIL. Agência Nacional de Vigilância Sanitária (BR). Portaria nº 30, de 13 de janeiro de 1998, que aprova o Regulamento Técnico referente a Alimentos para Controle de Peso. Brasília, DF: ANVISA.
CARITÁ, R.; MARIN-MORALES, M. A. Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of seeds to industrial effluents contaminated with azo dyes. Chemosphere, v. 72, n. 5, p. 722-725, 2008.
CARVALHO, F. R.; MOURA, A. G.; RODRIGUES, G. F.; NUNES, N. M.; LIMA, D. J.; PESSOA, C.; PERON, A. P. Are salty liquid food flavorings in vitro antitumor substances? Anais da Academia Brasileira de Ciências, v. 88, n. 3, p. 1419-1430, 2016.
CARVALHO, B. D. L.; SALES, I. M. S.; PERON A. P. Cytotoxic, genotoxic and mutagenic potential of UHT whole milk. Ciência e Tecnologia de Alimentos (Campinas), v. 37, n. 2, p. 275-279, 2017.
CAVICHIOLI, B.; ABOURIHAN, C. L. S.; PASSONI, C. M. S. Monitoramento da administração de um suplemento como coadjuvante na perda de peso. Saúde, v. 1, n. 1, p. 90-110, 2012.
FERNANDES, T. C. C.; MAZZEO, D. E. C.; MARIN-MORALES, M. A. Mechanism of micronuclei formation in polyploidizated cells of A. cepa exposed to trifluralin herbicide. Pesticide Biochemistry and Physiology, v. 88, n. 3, p. 252-259, 2007.
GOMES, K. M. S.; OLIVEIRA, M. V. G. A. D.; CARVALHO, F. R. D. S.; MENEZES, C. C.; PERON, A. P. Citotoxicity of food dyes sunset yellow (E-110), bordeaux red (E-123), and tatrazine yellow (E-102) on Allium cepa L. root meristematic cells. Ciência e Tecnologia de Alimentos (Campinas), v. 3, n. 1, p. 218-223, 2013.
GUERRA, M.; SOUZA, M. J. Como observar os cromossomos: um guia de técnicas em citogenética vegetal, animal e humana. Ribeirão Preto: FUNPEC.
GULTEKIN, F.; DOGUC, D. K.; KULAC, E. Effects of maternally exposed coloring food additivies on receptor expressions related to learning and memory in rats. Food Chemical and Toxicology, v. 56, p. 145-148, 2012.
HERRERO, O.; MARTÍN, J. P; FREIRE, P. F; LÓPEZ, L. C.; PEROPADRE, A.; HAZEN, M. J. Toxicological evaluation of three contaminant of emerging concern by use of Allium cepa test. Mutation Research, v. 743, n. 1-2, p. 24-34, 2012.
KRISHNA, K. A.; GOEL, S.; KRISHNA G. S. A. R. Genotoxicity and tumorigenicity predictions for 2-MI and 4-MI using multiple SAR software. Toxicology Mechanisms and Methods, v. 24, n. 4, p. 284-293, 2014.
LACERDA, L. P.; MALAQUIAS, G.; PERON, A.P. Antiproliferative action of aqueous extracts of Hymenaea stigonocarpa Mart. (Fabaceae) on the cell cycle of Allium cepa L. Anais da Academia Brasileira de Ciências, v. 89, n. 3, p. 1147-1150, 2014.
MARQUES, G.; DANTAS, E. B. S.; LIMA, L. H. G. M.; PERON, A. P. Ação do suco do fruto de Morinda citrifolia L. em células de sistema-teste vegetal. Biotemas, v. 27, n. 1, p. 43-146, 2015.
MORE, S. S.; RAZA, A.; VINCE, R. The butter flavorant, diacetyl, forms a covalent adduct with 2-deoxyguanosine, uncoils DNA, and leads to cell death. Journal of Agricultural of Food Chemistry, v. 60, n. 12, p. 3311-3317, 2012.
MOURA, A. G.; SANTANA, G. M.; FERREIRA, P. M. P.; SOUSA, J. M. C.; PERON, A. P. (2016). Cytotoxicity of Cheese and Cheddar Cheese food flavorings on Allim cepa L. root meristems. Brazilian Journal of Biology, v. 76, n. 2, p. 439-443, 2016.
NEVES, E. S.; FERREIRA, P. M. P.; LIMA, L. H. G. M.; PERON, A. P. Action of aqueous extracts of Phyllanthus niruri L.(Euphorbiaceae) leaves on meristematic root cells of Allium cepa L. Anais da Academia Brasileira de Ciências, v. 86, n. 3, p. 1131-1137, 2014.
OLIVEIRA, M. V. A.; ALVES, D. D. L.; LIMA, L. H. G. M.; CASTRO, J. M.; PERON, A. P. Cytotoxicity of erythrosine food colors (E-127), bright blue (E-133) and red 40 (E-129) In plant test system. Acta Scientiarum. Biological Science, v. 35, n. 1, p. 557-562, 2013.
RAJIV, S.; JEROBIN, J.; SARANYA, U.; NAINANAT, M.; SHARMA, A.; MARWANA, P.; MUKHERJEE A. Comparative cytotoxicity and genotoxicity of cobalt (II, III) oxide, iron (III) oxide, silicon dioxide, and aluminum oxide nanoparticles on human lymphocytes in vitro. Human & Experimental Toxicology, v. 35, n. 2, p. 170-18, 2016.
SALES, I. M. S.; SANTOS, F. K. S.; SILVA, F. C. C.; SOUSA, J. M. C.; PERON, A. P. Acute Toxicity of Grape, Plum and Orange Synthetic Food Flavourings Evaluated in in vivo Test Systems. Food Technology and Biotechnology, v. 55, n. 1, p. 131-137, 2017.
SANTANA, G. M.; DEUS, M. S. M.; SOUSA, J. M. C.; FERREIRA, P. M. P.; FERNANDES, H. B.; PERON, A. P. Antimitotic and antimutagenic action of the Hymenaea stigonocarpa bark on dividing cells. Brazilian Journal of Biology, v. 76, n. 1, p. 520-525, 2016.
SARDI, M.; HALDEMANN, H.; NORDMANN, B.; BOTTEX, B.; SAFFORD, B.; SMITH, B.; TENNANT, D.; HOWLETT, J.; JASTI, P. R. (2010). Use of retailer fidelity card schemes in the assessment of food additive intake: sunset yellow a case study. Food Additives and Contaminants Part A, v. 27, n. 11, p. 1507-1515, 2010.
SASAKI, Y. F.; KAWAGUCHI, S.; KAMAYA, A.; OHSHITA, M.; KABASAWA, K.; IWAMA, K.; TSUDA, S. The comet assay with 8 mouse organs: results with 39 currently used food additives. Mutation Research, v. 519, n. 1, p. 103-119, 2002.
SENGAR, G.; SHARMA, H. K. Food caramels: a review. Journal of Food Science and Technology, v. 51, n. 9, 1686-1696, 2014.
SILVA, S. I. O.; SANTANA, G. M.; SALES, I. M. S.; SOUSA, J. M. C.; PERON, A. P. Toxicity in food flavoring at the cellular level associated with each other at diferente doses. Acta Scientiarum. Biological Science, v. 38, n. 1, p. 77-84, 2016.
SOUSA, L. C.; SILVA, N. A.; BOULHOSA, R. B. Avaliação da composição nutricional através da rotulagem de shakes utilizados como substitutos de refeições. Revista Eletrônica Estácio Saúde, v. 5, n. 2, p. 78-90, 2016.
TABREZ, S.; SAHKIL, S.; UROOJ, M.; DAMANHORI, G. A.; ABUZENADAH, A. M.; AHMAD, D. Genotoxicity testing and biomarker studies on surface water: an over view of the techniques and their efficacies. Journal of Environmental Science and Health Part A, v. 29, n. 3, p. 250-257, 2011.
TONETTO, A.; HUANG, A.; YOKO. J.; GONÇALVES, R. O uso de aditivos de cor e sabor em produtos alimentícios. São Paulo: Faculdade de Ciências Farmacêuticas, 2008.
VAN EYK A. D. The effect of five artificial sweeteners on Caco-2, HT-29 and HEK-293 cells. Drug Chemical and Toxicology, v. 38, n. 3, p. 318-327, 2015.
WHITTAKER, P.; CLARKE, J. J.; SAN, R. H.; BEGLEY, T. H.; DUNKEL, V. C. Evaluation of the butter flavoring chemical diacetyl and a fluorochemical paper additive for mutagenicity and toxicity using the mammalian cell gene mutation assay in L5178Y mouse lymphoma cells. Food Chemical and Toxicology, v. 46, n. 8, p. 928-2933, 2008.
Downloads
Published
How to Cite
Issue
Section
License
To access the DECLARATION AND TRANSFER OF COPYRIGHT AUTHOR’S DECLARATION AND COPYRIGHT LICENSE click here.
Ethical Guidelines for Journal Publication
The Ciência e Natura journal is committed to ensuring ethics in publication and quality of articles.
Conformance to standards of ethical behavior is therefore expected of all parties involved: Authors, Editors, Reviewers, and the Publisher.
In particular,
Authors: Authors should present an objective discussion of the significance of research work as well as sufficient detail and references to permit others to replicate the experiments. Fraudulent or knowingly inaccurate statements constitute unethical behavior and are unacceptable. Review Articles should also be objective, comprehensive, and accurate accounts of the state of the art. The Authors should ensure that their work is entirely original works, and if the work and/or words of others have been used, this has been appropriately acknowledged. Plagiarism in all its forms constitutes unethical publishing behavior and is unacceptable. Submitting the same manuscript to more than one journal concurrently constitutes unethical publishing behavior and is unacceptable. Authors should not submit articles describing essentially the same research to more than one journal. The corresponding Author should ensure that there is a full consensus of all Co-authors in approving the final version of the paper and its submission for publication.
Editors: Editors should evaluate manuscripts exclusively on the basis of their academic merit. An Editor must not use unpublished information in the editor's own research without the express written consent of the Author. Editors should take reasonable responsive measures when ethical complaints have been presented concerning a submitted manuscript or published paper.
Reviewers: Any manuscripts received for review must be treated as confidential documents. Privileged information or ideas obtained through peer review must be kept confidential and not used for personal advantage. Reviewers should be conducted objectively, and observations should be formulated clearly with supporting arguments, so that Authors can use them for improving the paper. Any selected Reviewer who feels unqualified to review the research reported in a manuscript or knows that its prompt review will be impossible should notify the Editor and excuse himself from the review process. Reviewers should not consider manuscripts in which they have conflicts of interest resulting from competitive, collaborative, or other relationships or connections with any of the authors, companies, or institutions connected to the papers.
