Analysis of genetic diversity in two plantations of <i>Pinus caribaea</i> var. <i>hondurensis</i>

Autori

DOI:

https://doi.org/10.5902/1980509840930

Parole chiave:

Caribbean pine, Agroforestry, Biotechnology, SSRs (Short Sequence Repeats)

Abstract

Forest tree breeding activities in plantations with exotic species implies several instances of material selection where genetic variation can be affected. The objective of the work was to verify the genetic variability present in two plantations of Pinus caribaea var. hondurensis by microsatellite markers, initially designed for Pinus taeda fluorescent-labeled. Thus, 299 individuals were analyzed using eight polymorphic microsatellite markers. The results indicate that both plantations have adequate levels of genetic diversity that are representative of the Pinus genus. Through the Bayesian method, it was possible to detect two different population genetic structures (K = 2) between both plantations analyzed. In conclusion, this study suggests that microsatellites markers are useful tools to monitor genetic variation in genetic breeding programs. The genetic diversity estimated in both plantations is similar and typical for Pinus plantations, and as expected, there was a slight decrease in genetic variability in the commercial plantation in comparation with the base plantation.

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Biografie autore

Patricia Griselda Schmid, Estación Experimental Agropecuaria Montecarlo, Misiones

Ma. em Genética Vegetal, Pesquisadora, Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Montecarlo, Av. El Libertador 2472, Misiones, Argentina.

Martin Nahuel Garcia, Instituto de Agrobiotecnología y Biología Molecular, Buenos Aires

Pesquisador, Dr. pela Universidade de Buenos Aires, Instituto de Agrobiotecnología y Biología Molecular (IABiMo), INTA-CONICET, De Los Reseros y Dr. Nicolás Repetto s/n, 1686, Hurlingham, Buenos Aires, Argentina.

 

Maria Elena Gauchat, Estación Experimental Agropecuaria Montecarlo, Misiones

Pesquisadora, Dra. em Ciências Florestais e da Madeira, Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Montecarlo, Av. El Libertador 2472, Montecarlo, Misiones, Argentina.

 

Rafael Scherer, PINDO S.A., Misiones

Dr. em Ciências Agrárias, Gerente, PINDO S.A., Cl. Juan Domingo Perón 303, Puerto Esperanza, Misiones, Argentina

 

Hugo Daniel Reis, PINDO S.A., Misiones

Engenheiro Florestal, Gerente, PINDO S.A., Cl. Juan Domingo Perón 303, Puerto Esperanza, Misiones, Argentina.

Leonardo Gallo, Estación Experimental Agropecuaria Bariloche, San Carlos de Bariloche

Dr. em Ciências Florestais, Pesquisador em Genética e Melhoramento Florestal, Instituto Nacional de Tecnología Agropecuaria (INTA), Estación Experimental Agropecuaria Bariloche, Modesta Victoria 4450, San Carlos de Bariloche, Argentina.

Susana Noemi Marcucci Poltri, Instituto de Agrobiotecnología y Biología Molecular, Buenos Aires

Dra. em Ciências Biológicas, Pesquisadora, Instituto de Agrobiotecnología y Biología Molecular (IABiMo), INTA-CONICET, N. Repetto y De las Cabañas s/n (1686), Hurlingham, Buenos Aires, Argentina.  

Riferimenti bibliografici

AGUIAR, V. A.; SOUSA, V. A.; FRITZSONS, E.; JUNIOR, J. E. Programa de melhoramento de Pinus da Embrapa Florestas. Embrapa Florestas, Colombo, Brasil, p. 1-81. 2011.

BAGNOLI, F.; VENDRAMIN, G. G.; BUONAMICI, A.; DOULIS, A.; GONZÁLEZ‐MARTÍNEZ, S.C.;

LA PORTA, N.; MAGRI, D.; RADDI, P.; SEBASTIANI, F.; FINESCHI, S. Is Cupressus sempervirens native in Italy? An answer from genetic and palaeobotanical data. Molecular Ecology, [s.l.], v. 18, p. 2276 –2286. 2009.

DAÏNOU, K.; BIZOUX, J. P.; DOUCET, J. L.; MAHY, G.; HARDY, O.J.; HEUERTZ, M. Forest refugia revisited: SSRs and cpDNA sequence support historical isolation in a wide-spread African tree with high colonization capacity, Milicia excelsa (Moraceae). Molecular Ecology, [s.l.], v. 19, p. 4462–4477. 2010.

ANDRADE FURLAN, R.; SEIZO MORI, E.; VAGNER TAMBARUSSI, E.; BUENO DE MORAES, C.; ALMEIDA DE JESUS, F.; ZIMBACK, L. Estrutura genética de populações de melhoramento de Pinus caribaea var. hondurensis por meio de marcadores microssatélites. Bragantia, [s.l.], v. 66, n. 4, p. 553–563. 2007.

DELGADO, P.; PIÑERO, D.; REBOLLEDO, D.; JARDÓN-BARBOLLA, L.; CHI MAY, F. Genetic variation and demographic contraction of the remnant populations of Mexican Caribbean pine (Pinus caribaea var. hondurensis: Pinaceae). Annals of Forest Science, Mexico, v. 68, p. 121- 128. 2011.

DVORAK, W.; POTTER, K. M.; HIPKINS, V. D.; HODGE, G. R. Genetic diversity and gene exchange in Pinus oocarpa, a mesoamerican pine with resistance to the pitch canker fungus (Fusarium circinatum). Int. J. Plant. Sci, USA, v. 170, n. 5, p. 609–626. 2009.

GRATTAPAGLIA, D.; RESENDE, M. D. Genomic selection in forest tree breeding. Tree Genetics & Genomes, Springer-Verlag, v. 7, p. 241. 2011.

GONZÁLEZ-MARTÍNEZ, S. C.; DUBREUIL, M.; RIBA, M.; VENDRAMIN, G.G.; SEBASTIANI, F.; MAYOL, M. Spatial genetic structure of Taxus baccata L. in the western Mediterranean Basin: past and present limits to gene movement over a broad geographic scale. Mol Phyl Evol, [s.l.], v. 55, p. 805–815. 2010.

ISIK, F. Genomic selection in forest tree breeding: the concept and an outlook to the future. New Forests, USA, v. 45, p. 379. 2014.

KALIA, R.K.; RAI, M. K.; KALIA, S.; SINGH, R.; DHAWANET, A. K. Microsatellite markers: an overview of the recent progress in plants. Euphytica, [s.l.], v. 177, p. 309. 2011.

ELSIK, C. G.; MINIHAN, V.; HALL, S.; SCARPA, A.; WILLIAMS, C. Low-copy microsatellite markers for Pinus taeda L. Genome, Canada, v. 43, n. 3, p. 550-555. 2000.

ELSIK, C.; WILLIAMS, C.; AUCKLAND, L. Low-copy microsatellite recovery in a conifer genome. Theor. Appl. Genet, Canada, v. 103, p. 1189-1195. 2001.

HUBISZ, M.; FALUSH, D.; STEPHENS, M.; PRITCHARD, J. K. Inferring weak population structure with the assistance of sample group information. Molecular Ecology Resources, [s.l.], v. 9, p. 1322–1332. 2009.

KUTIL, B. L.; WILLIAMS, C. G.; AUCKLAND, L. Triplet-repeat microsatellites shared among hard and soft pines. J. Hered, [s.l.], v. 92, n. 4, p. 327-332. 2001.

MARCUCCI POLTRI, S.; GALLO, L.; ZELENER, N.; TORALES, S.; SHARRY, S. Biotecnología y Mejoramiento vegetal II. Parte V: Ejemplos de aplicaciones de la biotecnología vegetal. INTA Editions, Argentina, p. 435. 2010.

NEALE, D. B; WHEELER, N. C. The Conifers: Genomes, Variation and Evolution. Springer Nature, Switzerland. 2019.

SANCHEZ, M. INGROUILLE, M.J.; COWAN, R.S.; HAMILTON, M.A.; FAY, M.F. Spatial structure and genetic diversity of natural populations of the Caribbean pine, Pinus caribaea var. bahamensis (Pinaceae), in the Bahaman archipelago. Botanical Journal of the Linnean Society, United Kingdom, v. 174, p. 359–383. 2014.

SHEPHERD, M.; CROSS, M.; MAGUIRE, T.; DIETERS, M.; WILLIAMS, C.; HENRY, R. Transpecific microsatellites for hard pines. Theoretical and Applied Genetics, [s.l.], v. 104, p. 819- 827. 2002.

SEBASTIANI, F.; PINZAUTI, F.; KUJALA, S.; GONZÁLEZ-MARTÍNEZ, S. C; VENDRAMIN, G. G. Novel polymorphic nuclear microsatellite markers for Pinus sylvestris L. Conservation Genet. Resour, [s.l.], n. 4, p. 231. 2012.

PEAKALL, R., SMOUSE, P. GENALEX 6.4: Genetic Analysis in Excel. Population genetic software for teaching and research. Molecular Ecology, n. 6, p. 288– 295. 2006.

PRITCHARD, J. K.; STEPHENS, M.; DONNELLY, P. Inference of population structure using multilocus genotype data. Genetics, n. 155, p. 945–959. 2000.

SOTO, A.; ROBLEDO-ARNUNCIO, J. J.; GONZÁLEZ-MARTÍNEZ, S.C.; SMOUSE, P.E.; ALÍA, R. Climatic niche and neutral genetic diversity of the six Iberian pine species: a retrospective and prospective view. Molecular Ecology, [s.l.], n. 19, p. 1396–1409. 2010.

TAMBARUSSI, E. V.; SEBBENN, A. M.; TEIXEIRA DE MORAES, M. L.; ZIMBACK, L.; CAMACHO, E.; SEIZO MORI, E. Estimative of genetic parameters in progeny test of Pinus caribaea Morelet var. hondurensis Barret & Golfari by quantitative traits and microsatellite markers. Bragantia, Campinas, v. 69, n. 1, p. 39-47. 2010.

VILLALBA, P. Determinación de la Diversidad Genética en Huertos Semilleros Clonales de Pinus taeda L. mediante microsatélites. 2010. Thesis (Bachelor Degree in Genetics). University of Morón, Buenos Aires, Argentina. 2010.

ZHOU, Y.; BUI, T.; AUCKLAND, L.; WILLIAMS, C. Undermethylated DNA as a source of microsatellites from a conifer genome. Genome, Canada, v. 45, p. 91-99. 2002.

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Pubblicato

2022-11-23

Come citare

Schmid, P. G., Garcia, M. N., Gauchat, M. E., Scherer, R., Reis, H. D., Gallo, L., & Poltri, S. N. M. (2022). Analysis of genetic diversity in two plantations of <i>Pinus caribaea</i> var. <i>hondurensis</i>. Ciência Florestal, 32(4), 1759–1771. https://doi.org/10.5902/1980509840930

Fascicolo

V. 32 N. 4 (2022)

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