PROTOCOL SUGGESTED FOR DNA EXTRACTION OF JABUTICABA TREES
DOI:
https://doi.org/10.5902/198050983241Keywords:
Plinia cauliflora, DNA isolation, CTAB methodAbstract
This study aimed to adapt the protocol described by Soto (2001), to test different sources (plant tissue) and to establish conditions for collection and pre-treatment of samples for extracting DNA from jabuticaba trees (Plinia cauliflora (DC.) Berg, Myrtaceae). For this purpose, developing and expanded leaves, cambium of the stem and leaf stalk were used. The protocol tested can be used for DNA extraction from jabuticaba trees, because it provided DNA isolation of good quality and allowed the amplification of DNA through of PCR (Polymerase Chain Reaction). The use of liquid nitrogen for the maceration of leaves is necessary to prevent oxidation.
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References
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