DOES THE SAMPLE RANGE INTERFERE IN THE GERMINATION MEASUREMENTS OF Bowdichia virgilioides Kunth?
DOI:
https://doi.org/10.5902/1980509812346Keywords:
seed testing, physiological quality, sucupira-preta, optimum sample size.Abstract
http://dx.doi.org/10.5902/1980509812346
It is usual to find papers about seed germination of native species using different sample sizes. However, theconsequences of these procedures on the results are unknown. This study measured the germination processof Bowdichia virgilioides based on different seed sample sizes. The assay was conducted in a germinationchamber under continuous fluorescent white lamps and 26.2 ± 2.5 ºC. The design was completelyrandomized, as a 3 x 3 factorial (three sample sizes x three seed lots), with four replications per treatment.The sowing was done in gerbox, each one containing 25 seeds, making up 100, 200 and 400 seeds persample analyzed. Germinability, time (first, average and final), speed (mean germination rate and Maguire’sindex – VE or IVG), coefficient of variation of the germination time, uncertainty and synchrony of thegermination process were evaluated. Those seeds with embryo protrusion were considered germinated.Germination relative frequency graphics were also made. Among these measurements, the most fragile to size sample were VE and the uncertainty. VE was also influenced by the relation between germinability andmean germination rate, demonstrating its instability to measure the speed of the germination process whenthe lots have different germination capacity. Results of time measurements, uniformity, mean germinationrate and germinability were not affected by seed sample size. Germinability, however, was the most stablegermination measurement, and was not affected by sample size, even when analyzed by seed lots with verydiscrepant quality.
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References
ALBUQUERQUE, K. S. et al. Alterações fisiológicas e bioquímicas durante a embebição de sementes de sucupira-preta (Bowdichia virgilioides Kunth.). Revista Brasileira de Sementes, Londrina, v. 31, n. 1, p.012-019, 2009.
ALBUQUERQUE, K. S.; GUIMARÃES, R. M. Comportamento fisiológico de sementes de sucupira-preta (Bowdichia virgilioides Kunth.) sob diferentes temperaturas e condições de luz. Cerne, Lavras, v. 13, n. 1, p. 64-70, 2007.
ANDRADE, A. C. S. et al. Quebra de dormência de sementes de sucupira-preta. Pesquisa Agropecuária Brasileira, Brasília, v. 32, n. 5, p. 465-469, 1997.
AOSA. Association of Official Seed Analysts Inc.: home. Disponível em . Acessado em: 07 de julho de 2011.
BASKIN, C. C.; BASKIN, J. M. Seeds: Ecology, biogeography and evolution of dormancy and germination. San Diego: Academic Press, 1998. 666 p.
BRASIL. Regras para Análise de Sementes. Brasília: MAPA/ACS, 2009. 399 p.
BROWN, R. F.; MAYER, D. G. Representing cumulative germination: A critical analysis of single-value germination indices. Annals of Botany, Oxford, v. 61, p. 117-125, 1988.
CARVALHO, L. R. et al. Classificação de sementes florestais quanto ao comportamento no armazenamento. Revista Brasileira de Sementes, Londrina, v. 28, n. 2, p. 15-25, 2006.
CARVALHO, P. E. R. Espécies arbóreas brasileiras. Brasília: Embrapa Informação Tecnológica, 2006. 627 p. (Coleção Espécies Arbóreas Brasileiras, v.2).
COSTA, I. R. et al. Flora e aspectos auto-ecológicos de um encrave de cerrado nachapada do Araripe, Nordeste do Brasil. Acta Botanica Brasilica, Feira de Santana, v. 18, n. 4, p. 759-770, 2004.
FAO. Food and Agriculture Organization. Global Forest Resources Assessment. Roma: FAO-Forest Department, 2010. 12 p.
FIGLIOLIA, M. B. et al. Análise de sementes. In: AGUIAR, I. B. de; PIÑA-RODRIGUES, F. C. M.; FIGLIOLIA, M. B. Sementes Florestais Tropicais. Brasília: ABRATES, 1993.
ISTA. International Seed Testing Association – ISTA: home. Disponível em . Acessado em: 07 de julho de 2011.
LABOURIAU, L. G. A germinação das sementes. Washington: Secretaria Geral da organização dos Estados Americanos, Programa Regional de Desenvolvimento Científico e Tecnológico, 1983. 174 p.
LABOURIAU, L. G. On the physiology of seed germination in Vicia graminea Sm. I. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 42, p. 235-262, 1970.
LABOURIAU, L. G.; VALADARES, M. E. B. On the germination of seeds of Calotropis procera (Ait.) Ait. f. Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 48, p. 263-284, 1976.
MAGUIRE, J. D. Speed germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, Madson, v. 2, p. 176-177, 1962.
MELO, A. S. O que ganhamos ‘confundindo’ riqueza de espécies e equabilidade em um índice de diversidade? Biota Neotropical, Campinas, v. 8, n. 3, p. 21-27, 2008.
NOVEMBRE, A. D. L. C. Avaliação da qualidade de sementes. Seed News, Pelotas, v. 5, n. 3. p. 24-28, 2001.
RAMÍREZ, N; TRAVESET, A. Predispersal seed-predation by insects in the Venezuelan Central Plain: Overall patterns and traits that influence its biology and taxonomic groups. Perspectives in Plant Ecology, Evolution and Systematics, v. 12, p. 193-209, 2010.
RANAL, M. A. et al. Are there germination patterns for cerrado species?. In: DEL-CLARO, K.;. OLIVEIRA, P. S.; RICO-GRAY, V.; BARBOSA, A. A. A. (Org.). Encyclopedia of life support systems, tropical biology and natural resources. Oxford: Encyclopedia of Life Support Systems (Eolss) Publishers, 2009. v. 4, p.1575-1671.
RANAL, M. A.; SANTANA, D. G. How and why to measure the germination process? Revista Brasileira de Botânica, São Paulo, v. 29, n. 1, p. 1-11, 2006.
RODRIGUES, M. O. et al. Volatile constituents and antibacterial activity from seeds of Bowdichia virgilioides Kunt. Journal of Essential Oil Research, Carol Stream, v. 21, n. 3, p. 286-288, 2009.
SAMPAIO, L. S. V. et al. Ácido sulfúrico na superação da dormência de sementes de sucupira–preta (Bowdichia virgilioides H.B.K. - Fabaceae). Revista Brasileira de Sementes, Londrina, v. 23, n. 1, p. 184-190, 2001.
SANTANA, D. G.; RANAL, M. A. Análise da germinação: um enfoque estatístico. Brasília: Ed. da UnB, 2004. 248 p.
SAUTU, A. et al. Studies on the biology of 100 native species of trees in a seasonal moist tropical forest, Panama, Central America. Maryland, Forest Ecology Management, Amsterdan, v. 234, p. 245-263, 2006.
SCHEFFÉ, H. The analysis of variance. New York: Willey, 1959. 477 p.
SILVA JÚNIOR, I. F. et al. Antimicrobial screening of some medicinal plants from Mato Grosso Cerrado. Revista Brasileira de Farmacognosia, Curitiba, v. 19, n. 1, p. 242-248, 2009.
SILVA, D. D. et al. Diversidade populacional de Colletotrichum sublineolum em seis localidades no Brasil. Summa Phytopathologica, Botucatu, v. 34, n. 2, p. 149-155, 2008.
SMIDERLE, O. J.; SOUSA, R. C. P. Dormência em sementes de paricarana (Bowdichia virgilioides Kunth - Fabaceae - Papilionidae). Revista Brasileira de Sementes, Londrina, v. 25, n. 2, p. 48-52, 2003.
STEFANELLO, D. et al. Síndromes de dispersão de sementes em três trechos devegetação ciliar (nascente, meio e foz) ao longo do Rio Pindaíba, MT. Revista Árvore, Viçosa, v. 33, n. 6, p. 1051-1061, 2009.
STEINER, A. M. et al. Method validation in the early days – testing forest tree seeds in 1928-1934. Seed Testing International: Rules Development, Zurich, n. 138, p. 33-36, 2009.
STEINER, A. M.; KRUSE, M. History of seed testing. In: Seed Testing International (ISTA), Zurich, n.132, p.19-21, 2006.
THOMAZZI, S. M. et al. Antinociceptive and anti-inflammatory activities of Bowdichia virgilioides(sucupira). Journal of Ethnopharmacology, Konstanz, v. 127, p. 451-456, 2010.
URAMOTO, K. et al. Análise quantitativa e distribuição de populações de espécies de Anastrepha (Diptera: Tephritidae) no Campus Luiz de Queiroz, Piracicaba, SP. Neotropical Entomology, Londrina, v. 34, n. 1, p. 33-39, 2005.
WIELEWICKI, A. P. et al. Proposta de padrões de germinação e teor de água para sementes de algumas espécies florestais presentes na região sul do Brasil. Revista Brasileira de Sementes, Londrina, v. 28, n. 3, p. 191-197, 2006.
ZAIDAN, L. B. P.; CARREIRA, R. C. Seed germination in Cerrado species. Brazilian Journal of Plant Physiology, Campos dos Goytacazes, v. 20, n. 3, p. 167-181, 2008.
