Control of Meloidogyne javanica with aqueous extract of Trichilia claussenii
DOI:
https://doi.org/10.5902/2179460X89957Keywords:
Nematodes, Plant extract, Egg hatching, Lactuca sativa L.Abstract
Root-knot nematodes (Meloidogyne javanica) cause severe damage to numerous crops, highlighting the need for sustainable management strategies. This study aimed to evaluate the in vitro effect of the aqueous extract of Trichilia claussenii on the hatching of M. javanica eggs and to assess its influence on the initial development of lettuce (Lactuca sativa L.). The extract was tested at different concentrations (0–10%) and egg hatching was quantified five days after incubation by counting second-stage juveniles (J2) in microtiter plates. The same extract concentrations were applied to lettuce seedlings to evaluate potential phytotoxic effects on plant length and green mass after 14 days of growth. The results revealed a linear increase in nematicidal efficiency with rising extract concentration, reaching more than 60% inhibition of egg hatching at 4% and around 80% at higher doses. However, increasing concentrations negatively affected lettuce growth parameters. The 4% concentration presented an optimal balance between nematode control and minimal phytotoxicity. These findings demonstrate the potential of T. claussenii extract as a natural alternative for integrated nematode management, reducing reliance on synthetic nematicides and contributing to more sustainable agricultural systems.
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