Mutagenicity of Piraí river water – RJ, using Drosophila melanogaster warts (wts) as a bioindicator
DOI:
https://doi.org/10.5902/2179460X86796Keywords:
Genotoxic, Mutagenesis, Water qualityAbstract
The Piraí River is part of the Paraíba do Sul–Guandu watershed, which supplies water to over 80% of the population of the Rio de Janeiro metropolitan region. Along its course, the aforementioned river passes through a highly industrialized region, where it receives untreated urban sewage. Previous studies conducted with water samples deriving from Piraí River, based on using Allium cepa assay, have found cytogenotoxic compounds in them. This study aims to assess the mutagenic potential of genotoxic agents in water, using larvae of the Drosophila melanogaster Warts (wts) mutant strain as bioindicator. Analyses have investigated the incidence of epithelial tumors in the eyes, head, body, legs and halter of flies subjected to different treatments (positive control - doxorubicin, negative control – distilled water, and two water samples collected from Piraí River). Statistical analysis results have shown that the frequency of epithelial tumors in flies subjected to treatment with water samples was significantly higher than that of the negative control. This finding has evidenced the incidence of mutagenic agents in water and emphasized the importance of adopting sanitation strategies and of supervising industrial waste disposal. D. melanogaster Warts model proved to be a useful bioindicator to analyze mutagenic agents found in water samples.
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